JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2022
·Vol. 169
·期 5
·056520
·
IF 4.386
Yarui An,Lin Song,Xiaoyan Chen,Chenming Ni,Kuanzheng Mao,Leijing Zhu,Yingying Gu,Yuqing Miao,Bin Song,Hongyun Ma
This work took advantage of 3D biomimetic hydrangea-like bismuth oxychloride (BiOCl) and PtNi nanocubes to fabricate a facile electrochemical immunosensor for the detection of CA19–9. Herein, APTES functionalized BiOCl (APTES-BiOCl) with large specific surface areas can provide eco-friendly microenvironment for biomolecules. The PtNi nanocubes prepared by the classic hydrothermal method have good conductivity, which can facilitate electron transfer highly efficient. Meanwhile, PtNi nanocubes can immobilize the anti-CA19–9 via covalent Pt-N bonds to anchor a great number of biomolecules more firmly. In addition, reduced graphene oxide (rGO) with excellent conductivity was implemented as a matrix, which greatly promoted electron transfer at the sensing interface. Under the optimal conditions, the proposed immunosensor can detect CA19–9 sensitively, from which a linear range of 0.01–150 U·ml–1 was derived accompanied with a low detection limit of 0.0034 U·ml–1. The results illustrated that the designed immunosensor exhibited excellent specificity, good reproducibility, and satisfactory stability. Moreover, it can be applied in the detection of real samples, which indicated that the immunosensor has great potential for early screening and diagnosis of diseases.
研究领域:
材料科学
·
电化学
·
生物传感器
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2023
·
IF 4.3
Qiwen Feng,Yumeng Liu,Liping Zou,Mengying Lei,Changhong Zhu,Wei Xia
Fluorene-9-bisphenol (BHPF) is an emerging global endocrine-disrupting chemical found in numerous household products as a substitute of bisphenol A. Many studies have reported various toxicities associated with BHPF. However, the effect of BHPF on male reproduction, particularly on the structural integrity of the blood testis barrier (BTB) in mice, has not yet been extensively studied. Ferroptosis , a newly identified form of cell death, occurs in the testicular tissue following exposure to BPA, affecting male fertility. We investigated whether ferroptosis plays a role in BHPF-induced testicular damage. The findings indicated that BHPF exposure led decreases in serum testosterone (T) concentration and sperm concentration and motility in mice. Furthermore, BHPF disrupted the BTB by interfering with key BTB-related proteins, including Cx43, β-catenin, and ZO-1. Moreover, BHPF induced ferroptosis through the induction of lipid peroxidation, iron overload, oxidative stress , and mitochondrial dysfunction in the testicular tissue. Inhibition of ferroptosis using Fer-1 mitigated the BHPF-induced damage to the BTB and ferroptosis in TM4 cells. Overall, our findings indicated the detrimental effects of BHPF on male reproductive function in mice, suggesting ferroptosis as a mechanism underlying testicular damage.
研究领域:
毒理学
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生殖生物学
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内分泌学
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DOI
PubMed
高影响因子
Journal of Environmental Sciences
·
2023
·
IF 6.9
Shuhui Zhang,Xiang Zuo,Jialu Luan,Huijuan Bai,Zhenhua Fu,Mingzhu Sun,Xin Zhao,Xizeng Feng
Increasing evidence indicates that disturbance of the clock genes, which leads to systemic endocrine perturbation, plays a crucial role in the pathogenesis of metabolic and liver diseases. Fluorene-9-bisphenol (BHPF) is utilized in the manufacturing of plastic materials but its biological effects on liver homeostasis remain unknown. The impacts and involved mechanisms of BHPF on the liver diseases, metabolism, and circadian clock were comprehensively studied by zebrafish and mouse models. The therapeutic effect of melatonin (MT) was also verified. Zebrafish and mouse models with either acute exposure (0.5 and 1 µmol/L, 1-4 days post-fertilization) or chronic oral exposure (0.5 and 50 mg/(kg·2 days), 30 days) were established with various BHPF concentrations. Herein, we identified a crucial role for estrogenic regulation in liver development and circadian locomotor rhythms damaged by BHPF in a zebrafish model. BHPF mice showed chaos in circadian activity through the imbalance of circadian clock component Brain and Muscle Aryl hydrocarbon receptor nuclear translocator-like 1 in the liver and brain. The liver sexual dimorphic alteration along with reduced growth hormone and estrogens played a critical role in damaged glucose metabolism, hepatic inflammation, and fibrosis induced by BHPF. Besides, sleep improvement by exogenous MT alleviated BHPF‐related glucose metabolism and liver injury in mice. We proposed the pathogenesis of metabolic and liver disease resulting from BHPF and promising targeted therapy for liver metabolism disorders associated with endocrine perturbation chemicals. These results might play a warning role in the administration of endocrine-disrupting chemicals in everyday life and various industry applications.
研究领域:
毒理学
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内分泌学
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药理学
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分子生物学
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DOI
高影响因子
JOURNAL OF THE AMERICAN CERAMIC SOCIETY
·
2018
·Vol. 102
·期 7
·4092-4102
·
IF 2.956
Yunhui Zhang,Haitao Wu
La 2 (Zr 1− x Ti x ) 3 (MoO 4 ) 9 (0 ≤ x ≤ 0.1) ceramics were prepared by the traditional solid-state reaction method. XRD analysis showed that La 2 (Zr 1− x Ti x ) 3 (MoO 4 ) 9 (0 ≤ x ≤ 0.1) ceramics belonged to a trigonal system. Based on the chemical bond theory, the consequences of bond energy, bond ionicity, lattice energy, and thermal expansion coefficient of ceramics on microwave dielectric properties were discussed. As Ti 4+ addition was increased, the reduction in dielectric constant was ascribed to the fact that the polarizability of Ti 4+ is smaller than Zr 4+ , and the downward trend was related to the bond ionicity. Besides, the tendency of Q·f value depended on the packing fraction and the lattice energy. The improvement in τ f value, the increase in bond energy, and the decrease in the coefficient of thermal expansion were all correlated. The far-infrared spectra implied that the absorptions of structural phonon oscillation were the main reason for the maximum polarization contribution. La 2 (Zr 0.92 Ti 0.08 ) 3 (MoO 4 ) 9 ceramics sintered at 750°C for 4 hours exhibited the best dielectric properties ( ε r = 10.33, Q·f = 80 658 GHz, and τ f = 3.48 ppm/°C).
研究领域:
材料科学
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陶瓷工程
·
电气工程
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DOI
高影响因子
FUEL
·
2022
·
IF 8.035
Xiaodong Wang,Xiaoran Li,Sen Liu,Haofei Zhou,Qiuye Li,Jianjun Yang
Cis-9-Octadecenylamine modified α-Fe 2 O 3 and Fe(OH) 3 nanoparticles were successfully synthesized by using a one-step synthetic method. XRD results revealed that lower ratio of oleylamine to Fe 3+ introduce forming hematite phase but higher ratio introduce forming Fe(OH) 3 phase. FTIR, TGA, contact angle, and XPS results indicated that olelyamine were modified on the surface of α-Fe 2 O 3 or Fe(OH) 3 . A larger amount of oleylamine modifier is conducive to the dispersion of the catalysts in heavy oil. Oleylamine modified Fe(OH) 3 can be transformed into α-Fe 2 O 3 in situ during the catalytic reaction and shows higher catalytic activity in the viscosity reduction of Shengli extra heavy crude oil.
研究领域:
化学工程
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材料科学
·
石油工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY OF MATERIALS
·
2019
·
IF 10.159
Siming Yu,Rong Wen,Haiyang Wang,Yongchao Zha,Lin Qiu,Bo Li,Wei Xue,Dong Ma
Au nanoclusters (Au NCs) are materials that hold great promise in biomedical applications due to their improved photophysical properties. However, the current nonthiolate polymer-based self-assembly methods for preparing Au NCs face drawbacks of usage of environmentally unfriendly reducing reagents or nonbiocompatible polymers, which largely hamper their more extensive biomedical use. In this work, biocompatible chitosan-graft-poly(l-lysine) (CPL) dendrons are designed and synthesized based on a simple azide–alkyne cyclization reaction for facile preparation of Au@CPL NCs in one step. The obtained Au@CPL NCs exhibited enhanced computer tomography imaging property as compared with clinically used iohexol (Omnipaque) and displayed high delivery efficiency for matrix metalloproteinase-9 plasmid shRNA (pMMP-9). After surface functionalization by cyclic arginine-glycine-aspartic (Arg-Gly-Asp, RGD), a peptide that specifically targets αvβ3 integrin overexpressed by cancer cells, the resultant Au@CPL-RGD/pMMP-9 complex can precisely and efficiently inhibit tumor growth both in vitro and in vivo. Our work provides a novel strategy to fabricate Au NCs with enhanced biorelevant properties for more efficient cancer diagnosis and therapy.
研究领域:
生物医学工程
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分子生物学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2022
·
IF 9.965
Yun Cai,Haibo Shu,Fengjiao Yu,Yang Yang
Radial heterostructures with titanium dioxide (TiO 2 ) nanowire as core and NTU-9 as shell are synthesized via a surfactant-free approach based on the favorable bonding of linkers with TiO 2 nanowire. Relative to the traditional growth strategy of surface modification, the thin NTU-9 shell with ordered arrangement of two-dimensional networks is uniformly formed on the sidewalls of TiO 2 nanowire through the orientational growth process. Using the core–shell nanowire arrays as photoanodes , wide-range light absorption and high charge carrier separation efficiency are achieved due to the conjugation of NTU-9, leading to enhanced water oxidation performance in photoelectrochemical (PEC) water splitting. Under appropriately low applied potentials, the photogenerated holes are preferable to accumulate at TiO 2 /NTU-9/electrolyte three-phase interface and thus the long-range ordered NTU-9 shell can also serve as a size-exclusion filter to improve selectivity toward molecules of different sizes. Consequently, the TiO 2 /NTU-9 core–shell nanowire array exhibits an augmented and selective PEC response for small size molecules ( e.g., H 2 O 2 ) at a very low potential (–0.25 V vs Ag/AgCl), outperforming the pure TiO 2 nanowire array and the counterpart with a grain-boundary-rich NTU-9 shell that is prepared by pretreatment of the TiO 2 nanowires with PVP functionalization.
研究领域:
材料科学
·
光电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Qi Xiao,Dandan Guo,Jing He,Min Zhang,Shan He,Shaohua Huang
Background In reversed-phase liquid chromatography , the C 18 alkyl bonded phase, as the primary stationary phase, is widely used in pharmaceutical and food analysis . The phenyl bonded phase often serves as a complementary choice to the C 18 phase to enhance the separation performance of specific categories of compounds. However, both C 18 and the currently available phenyl bonded phase chromatography columns show room for further optimization in improving the separation efficiency of specific compound classes, such as dihydroflavonoids. Additionally, the potential role and impact of introducing phosphorus groups into chromatographic stationary phases have not been fully explored, indicating a promising direction for research. Results In the present work, we prepared a novel phenyl stationary phase by bonding 9-oxa-10-phosphaphenanthrene 10-oxide onto silica gel . The obtained material was characterized by scanning electron microscopy, fourier transforms infrared spectroscopy , and elemental analysis . The results show that 9-oxa-10-phosphaphenanthrene 10-oxide was successfully bonded on the silica surface with a load of 3.90 %. Further chromatographic characterization in high-performance liquid chromatography exhibited high column efficiency (40,792 plates m-1 for the determination of biphenyl) and good stability (RSD of 0.28 %∼5.38 %). Moreover, we made a detailed study of the column separation mechanism by nuclear magnetic resonance spectroscopy titration experiment. Comparing to commercial phenyl column, the proposed stationary phase showed shorter retention time and higher throughput. In addition, the stationary phase has a strong ability to separate multiple types of compounds, which provides a new strategy for the separation of complex samples, such as active ingredients in traditional Chinese medicine . Significance We have developed a novel phenyl column and conducted a comprehensive examination of its chromatographic performance, demonstrating excellent separation capabilities and high efficiency for both nonpolar and moderately polar aromatic compounds . Additionally, we explored the impact of phosphorus-containing groups on the separation performance of chromatographic stationary phases.
研究领域:
分析化学
·
药物分析
✓ 已验证
DOI
PubMed
高影响因子
MOLECULAR NEUROBIOLOGY
·
2023
·Vol. 60
·期 7
·3824-3838
·
IF 5.1
Deng Ling,Zhang Jiyu,Chen Sha,Wu Yu,Fan Xiaomei,Zuo Tianrui,Hu Qingwen,Jiang Lu,Yang Shaonan,Dong Zhi
Blood-brain barrier (BBB) disruption can induce further hemorrhagic transformation in ischemic stroke (IS). miR-671-5p, a micro-RNA, is abundant in the cortex of mammalian brains. Herein, we investigated the roles and potential mechanisms for the effects of miR-671-5p on BBB permeability in IS. Results showed that miR-671-5p levels were significantly downregulated in the cerebral cortex of middle cerebral artery occlusion/reperfusion (MCAO/R) C57/BL6 mice in vivo . miR-671-5p agomir administration via right intracerebroventricular injection significantly reduced infarct volume, improved neurological deficits, the axon of neurons and nerve fiber, attenuated cell injury and apoptosis, as well as reduced BBB permeability in MCAO/R mice. Treatment with miR-671-5p agomir alleviated tight junction proteins degradation, including claudin, occludin, and ZO-1 in MCAO/R mice, and these effects were reversed following NF-κB overexpression. Bend.3 brain endothelial cells were subjected to oxygen and glucose deprivation/reoxygenation (OGD/R) treatment in vivo , and then miR-671-5p agomir was transfected into the cells. This resulted in reduction of cytotoxicity, improved cell viability, trans-endothelial electrical resistance, reduced fluorescein sodium permeability, and inhibited tight junction degradation in Bend.3 OGD/R cells. However, these effects were reversed following NF-κB overexpression. These results demonstrated that upregulation of miR-671-5p in IS models in vivo and i n vitro alleviated BBB permeability by targeting NF-κB/MMP-9. In summary, miR-671-5p is a potential therapeutic target for protecting BBB permeability in IS to minimize cerebral hemorrhage transformation.
研究领域:
神经科学
·
分子生物学
·
药理学
·
病理生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2024
·Vol. 31
·期 13
·19396-19408
·
IF 0
Pan Enzhuang,Xin Yue,Li Xueqing,Ping Kaixin,Li Xing,Sun Ying,Xu Xuhui,Dong Jingquan
As a broad-spectrum and efficient triazole fungicide, difenoconazole is widely used, which not only pollutes the environment but also exerts toxic effects on non-target organisms. The spleen plays an important role in immune protection as an important secondary lymphoid organ in carp. In this study, we assessed the protective impact of silybin as a dietary additive on spleen tissues of carp during exposure to difenoconazole. Sixty carp were separated into four groups for this investigation including control group, difenoconazole group, silybin group, and silybin and difenoconazole group. By hematoxylin-eosin staining, dihydroethidium staining, immunohistochemical staining, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay, quantitative real-time PCR assay, Western blot analysis, biochemical assays, and immune function indicator assays, we found that silybin could prevent difenoconazole-induced spleen tissue damage, oxidative stress, and immune dysfunction, and inhibited apoptosis of carp spleen tissue cells by suppressing the formation of p53-driven caspase-9-apoptotic protease activating factor-1-cytochrome C complex. The results suggested that silybin as a dietary additive could improve spleen tissue damage and immune dysfunction induced by difenoconazole in aquaculture carp.
研究领域:
环境毒理学
·
免疫学
·
水产养殖学
✓ 已验证
DOI
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2022
·Vol. 194
·期 7
·3044-3065
·
IF 3.094
Qiu Ren,Dai Jianmin,Meng Lingqiang,Gao Hongmin,Wu Mengdie,Qi Feifan,Feng Jing,Pan Hongzhi
Porous carbon sphere materials have a large variety of applications in several fields due to the large surface area, adaptable porosity, and good conductivity they possess. Obtaining a steady carbon sphere using the green synthesis method remains a significant challenge. In this experiment, covalent organic frameworks (COFs) were used as a precursor and Fe 3 O 4 NPs were integrated into the precursor in order to synthesize a porous carbon sphere material using the one-step pyrolysis method. COFs have an ordered porous structure, perpetual porosity, large surface area, and low density and display good environmental tolerance. These properties make them an excellent precursor for synthesizing porous carbon sphere, which maintains good morphology at high temperatures, and it is not involved in the removal of dangerous reagent and small size restrictions during the synthesis process. In addition to the formation of a porous carbon sphere, transition metal carbon material that contains N element can be an active catalyst. The composites exhibit better activity when Fe is doped into carbon materials containing N element than that of other doped transition metals including Mn and Co. In this situation, the integration of Fe 3 O 4 NPs and N element in the COF precursor exposed the active sites of the composites and the two substances synergistically improved the electrocatalytic properties, and the composites were named Fe 3 O 4 @NPCS. The constructed Fe 3 O 4 @NPCS/GCE immunosensor was applied as a means of detecting CA19-9 antigen and presented a wide linear range from 0.00001 to 10 U/mL with a low detection limit of 2.429 μU/mL (S/N = 3). In addition, the prepared immunosensor was utilized for detecting CA19-9 antigen in the real human serum, and the recovery rates were in the range from 95.24% to 106.38%. Therefore, a porous carbon sphere prepared by COFs as a precursor can be applied for the detection of CA19-9 antigen in real samples, which could be an excellent strategy for CA19-9 antigen detection and could potentially promote the development of COF materials in various electrochemical fields.
研究领域:
分析化学
·
材料科学
·
生物传感器
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Rui Gao,Shen Zhang,Hailong Liu,Zhizhou Yang,Yue Wu,Sheng Gao,Mei Li,Hanlin Shao,Mingjie Wu
Disposal of dye-contaminated wastewater has become an imperative issue. Synthesis of novel materials for larger-capacity removal of those contaminants assisting by special interaction is a significant challenge. In this paper, a series of hyper-crosslinked poly 9-bromophenanthrene hybrids (HCPs) were synthesized by combination of 9-bromophenanthrene (9BP) and octavinylsilsesquioxane (OVS) via Friedel-Crafts alkylation reaction. The synthesized HCPs were well characterized. The results illustrated that HCPs were porous polymer hybrids consisting of micropores and mesopores with the apparent surface areas ( S BET ) in range of 980.0–1010.6 m 2 g −1 , and the micropore and mesopore widths located at 1.42 and 4 nm. Dye adsorption disclosed that the adsorbed amounts of rhodamine B (RB) and Congo red (CR) on HCP-2 respectively arrived at 2646.0 ± 37.4 and 1550.0 ± 38.5 mg g −1 , ascribing to the interactions of pore filling, hydrogen bonds, π π stacking, electrostatic attraction, especially the C Br⋯π interaction between the resulting polymers and the dye molecules of RB and CR. Moreover, HCP-2 exhibited larger adsorbed amount of iodine vapor arriving at 179.9 ± 2.6 wt%. This work provided an innovative strategy to design new adsorbents for larger-amount removal of contaminants from the environment.
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）