FITOTERAPIA
·
2023
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IF 3.4
Yang Liu,Limei Ran,Yahong Wang,Peng Wan,Hongli Zhou
The polysaccharides from Sea buckthorn leaves (SBLPs) were extracted by hot water and purified by DEAE cellulose , then separated into six polysaccharides (SBLP-S) by DEAE-52 column. Six separated polysaccharides were characterized by Ultraviolet Spectroscopy, Infrared Spectrum, High Performance Liquid Chromatographic and Congo red analysis. The antioxidant activity and immunological activity were investigated in vitro . The results revealed that the monosaccharide composition of SBLP-S-1, SBLP-S-2, SBLP-S-3, SBLP-S-5 and SBLP-S-6 contained Man , GlcN , Rib , Rha , GluA , GalA , Glu, Gal, Xyl , Ara and Fuc , among them, rare glucosamine was found. And SBLP-S-4 contained all above components except GlcN and GluA. FT-IR showed that SBLP-S were sulfated polysaccharide containing uronic acid . Molecular weights of SBLP-S were 338.659, 401.305, 599.849, 393.904, 626.895 and 176.862 kDa. The Congo-red test indicated that SBLP-S-2, SBLP-S-4, SBLP-S-5, and SBLP-S-6 had triple helix conformation. Crude polysaccharides had the strong scavenging activities on DPPH radicals, ABTS radicals and hydroxyl radicals . The six polysaccharides had the activity of immune stimulation on RAW264.7 cell. SBLP-S-2 promoted the phagocytosis best and SBLP-S-6 promoted the NO production best. The results suggested that SBLPs could be used as potential antioxidants and immunomodulatory agents in pharmaceutical and functional food fields.
研究领域:
药理学
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食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2023
·
IF 8.4
Jiahui Chen,Jiale Chai,Xiaomei Sun,Ye Tao,Xing Chen,Guanghong Zhou,Xinglian Xu
The efficient synthesis of myofibrillar protein(MRN)-gallic acid (GAD) complex in ultrasound (UID)-assisted processing is a challenging problem in food manufacturing. In this investigation, the effect of viscosity characteristics on the efficiency of UID processing in MRN-based beverages was analyzed. Both viscosity and surface tension can increase sono-physico-chemical effects on the degradation of terephthalic acid and crystal violet, with surface tension having a more significant effect (negative correlation, R 2 = 0.99) than viscosity (positive correlation, R 2 = 0.79). The structural indicators and microstructure demonstrated that the reaggregation and refolding of the MRN structure during the modification procedure occurred with relatively small three-dimensional dimensions. Compared to the MRN/GAD4 group, the water contact angle of the MRN/GAD7 system enhanced by 129.44%, leading to greater system stability. The ABTS-scavenging capacity of the system increased by approximately 19.45% due to the increase in viscosity of these two categories.
研究领域:
食品科学
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化学工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
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IF 4.8
Xiaoli Zhou,Yumeng Qin,Yanli Wang,Yaping Wang,Zhicheng Qin
Rosa roxburghii Tratt (RRT) is renowned for its abundance of phenolics, various methods have been used to extract free phenolics from RRT, there remains a paucity of research on the comparative distinctions between bound phenolic compounds and their free counterparts in RRT, especially in vitro and in vivo antioxidant potential of RRT-BP necessitates further exploration. This study sought to optimize the process parameters for extracting bound polyphenols through alkaline hydrolysis . Among the bound phenolic compounds, 20 phenolic acids and 34 flavonoids were identified, while the free phenolic compounds comprised 19 phenolic acids and 42 flavonoids. The IC 50 values for DPPH and ABTS radical scavenging were 44.92 μg/mL and 60.92 μg/mL for free phenols , 87.02 μg/mL and 95.02 μg/mL for bound phenols, respectively. Compared with the control group, the lifespan of Caenorhabditis elegans fed with 1 mg/mL of free and bound phenol extended from 11.63 d to 16.77 d and 13.88 d, respectively, with no adverse impact on the reproductive capability of the nematodes. The longevity of C. elegans was observed to persist even under thermal and oxidative stress , primarily attributed to elevated superoxide dismutase and catalase activity, coupled with reduced malondialdehyde content in C. elegans . These findings underscore that free polyphenols exhibit superior polyphenolic components and antioxidant properties compared to their bound counterparts. Nevertheless, the potential health effects of bound polyphenols as a supplement to free polyphenols in RRT should not be ignored.
研究领域:
药理学
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生物化学
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分子生物学
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营养学
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生物技术
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
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IF 5.7
Guangkai Wu,Zihan Zhao,Jun Hu,Yue Li,Jianxia Sun,Weibin Bai
The chemical synthesis of anthocyanins, especially delphinidin-3-O-glucoside and petunidin-3-O-glucoside, is preferable due to the challenges associated with their extraction and purification. However, the reported methods for the synthesis are scarce and intricate. Our research focused on exploring a one-step ester-to-ketone process and optimizing the ring formation reaction, simplifying and improving the overall synthesis strategy. Through these attempts, we were able to achieve higher production yields of delphinidin-3-O-glucoside and petunidin-3-O-glucoside. According to the results of DPPH, ABTS, and FRAP, the antioxidant activity of anthocyanins was increased with the number of B ring hydroxyl substituent. Additionally, both delphinidin-3-O-glucoside and petunidin-3-O-glucoside exhibited no cytotoxicity effects, highlighting their potential for safe application in various fields.
研究领域:
化学
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药学
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生物化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
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2019
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IF 2.923
Yi Xu,Jing Yang,Yao Lu,Ling-Ling Qian,Zhi-Yin Yang,Rui-Min Han,Jian-Ping Zhang,Leif H. Skibsted
Luteolin differs as a radical scavenger dramatically from apigenin in response to Cu(II) coordination despite a minor structural difference. Coordination of Cu(II) increases the radical scavenging efficiency of luteolin, especially at low pH, while decreases the efficiency of apigenin at both low and higher pH as studied by ABTS•+ radical scavenging. Luteolin forms a 1:1 complex with Cu(II) binding to 4-carbonyl and 5-phenol for pH <6 and to 3′,4′-catechol for pH >6. Apigenin forms a 1:2 complex independent of pH coordinated to 4-carbonyl and 5-hydroxylyl. Cu(II) coordinated to luteolin, as studied by pH jump stopped-flow, translocates with rate constants of 11.1 ± 0.3 s–1 from 4,5 to 3′,4′ sites and 1.0 ± 0.1 s–1 from 3′,4′ to 4,5 sites independent of Cu(II) concentration, pointing toward the dissociation of Cu(II) from an intermediate with two Cu(II) coordination as rate determining. 3′,4′-Catechol is suggested to be a switch for Cu(II) translocation with deprotonation initiating 4,5 to 3′,4′ translocation and protonation initiating 3′,4′ to 4,5 translocation. For dicoordinated apigenin, the coordination symmetry balances an electron withdrawal effect of Cu(II) resulting in a decrease of phenol acidity and less radical scavenging efficiency compared to parent apigenin. Compared to that of parent luteolin, the radical scavenging rate of both 4,5 and 3′,4′ Cu(II)-coordinated luteolin is enhanced through increased phenol acidity by electron withdrawal by Cu(II), as confirmed by density functional theory (DFT) calculations. Coordination and translocation of Cu(II) accordingly increases the antioxidant activity of luteolin at pH approaching the physiological level and is discovered as a novel class of natural molecular machinery derived from plant polyphenols, which seems to be of importance for protection against oxidative stress.
研究领域:
化学
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生物化学
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药理学
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植物科学
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产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
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2019
·Vol. 13
·期 4
·2790-2797
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IF 1.415
Han Yi-Tong,Zhao Chen-Chen,Han Jia-Run,Yan Jia-Nan,Du Yi-Nan,Shang Wen-Hui,Wu Hai-Tao
The purpose of the present study was to explore the antioxidant activity of organic extracts from Maillard reaction products (MRPs) of sea cucumber ( Stichopus japonicus ) gut hydrolysates (SCGHs). The SCGHs were obtained after treatment with neutrase, and they were then reacted with ribose at a mass ratio of 1:2, pH 8.0, and 95 °C for up to 12 h. The MRPs were extracted by methanol and ethanol. The methanol extracts of the MRPs had a higher UV absorbance, browning intensity, and fluorescence intensity than the crude MRPs. The scavenging capacities of methanol and ethanol extracts from MRPs in both 2′,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) assays increased significantly ( p < 0.05). The methanol extracts were better than the ethanol extracts showing the reduced IC 50 levels for ABTS and DPPH by 50% and 50%, respectively. The inhibitory activity for peroxidation of linoleic acid of MRPs was also enhanced in the methanol extracts of MRPs, but this was reduced in ethanol extracts. Moreover, HepG2 cell viability after exposure to the methanol extracts of MRPs was increased to 59.30 ± 0.43% compared with the SCGHs and MRPs, which was 3.97 times that of the H 2 O 2 -treated group. These results suggest that methanol extraction can improve the antioxidant activity of SCGHs-ribose MRPs and that the corresponding extracts can be potentially used as food antioxidants.
研究领域:
食品科学
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营养学
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生物化学
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药理学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Sustainable Food Systems
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2022
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IF 5.005
Dong Yuhe,Zhong Wenting,Yang Chunmiao,Zhang Yongze,Yang Dongsheng
The objectives of this study are to optimize the ultrasonic microwave synergistic extraction (UMSE) of anthocyanins from Lycium ruthenicum Murr (ALRM) by response surface methodology and also to investigate its antioxidant activity in vitro. Based on the single-factor experiments, we optimized the ALRM extraction process by response surface methodology assuming anthocyanin extraction rate as the response point and microwave power, ultrasonic power, extraction time, and liquid-to-material ratio as the investigating parameters as well as performed correlation analysis between DPPH·, ·OH, ABTS·, O2-· radical scavenging potential, and ferric-reducing antioxidant power. The results showed that the influence on anthocyanin yield was in the order of liquid to material ratio > microwave power > ultrasonic power > extraction time; the elicited extraction conditions were: ultrasonic power 216.253 W, microwave power 89.311 W, time 26.141 min, liquid to material ratio 17.294 mL/g, the forecast content was 10.157 mg/g, while the actual value was consistent with the preview value. Meanwhile, ALRM obtainable by UMSE was identified as yielding favorable antioxidant properties firstly, when the concentration of purified ALRM (ALRM-1) was increased, the antioxidant capacity was strengthened. Theoretically, we provide a basis for the extraction procedure of ALRM and its antioxidant activity, which serves as a promising antioxidant and free radical scavenger.
研究领域:
食品科学
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植物化学
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分析化学
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营养学
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产品关联
DOI
高影响因子
Food Packaging and Shelf Life
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2022
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IF 8.749
Pengquan Zhao,Jiandong Wang,Xinyu Yan,Zihui Cai,Lina Fu,Qi Gu,Lin Liu,Haojie Jin,Yujie Fu
In this study, the bioactive extract of Rosa roxburghii Tratt (RRT) leaves was incorporated into a chitosan/zein matrix to produce a food packaging film with higher antioxidant and antimicrobial activities. The natural deep eutectic solvent (NADES) was taken as the extraction solvent and green plasticizer to prepare the film matrix. The physical and structural properties of chitosan/zein films with different concentrations of RRT leaves extracts were characterized by infrared spectroscopy analysis , thermogravimetric analysis , X-ray diffraction and scanning electron microscope. The incorporation of RRT leaves extract significantly improved the mechanical properties , light barrier properties and water vapor permeability of the chitosan/zein films. Moreover, the films containing RRT leaves extract had better antioxidant activities and antibacterial activities, with the highest DPPH and ABTS radical scavenging activities of 88.17 % and 86.84 %, respectively, and also effectively inhibited the growth of microorganisms and extended the shelf life of blueberries and cherry tomatoes .
研究领域:
食品科学
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材料科学
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生物技术
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化学
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产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
·
IF 6
Rong-Shuai Hu,Li Yu,Sai-Ya Zhou,Hui-Fen Zhou,Hai-Tong Wan,Jie-Hong Yang
The natural deep eutectic solvent (NADES) was used as the extraction solvent to extract the four ingredients from Chuanxiong-Honghua. NADES-7 composed of L-proline and lactic acid (1:2) has a better extraction effect compared with traditional solvents. The extraction process was optimized using response surface methodology (RSM) and ant colony algorithm-enabled backpropagation neural network (ACO-BPNN), which were compared with each other. ACO-BPNN has a higher R 2 and predictive value than RSM. The optimal process parameters predicted by the ACO-BPNN were determined to be as follows: solvent-to-material ratio, 8 mL/g; extraction time, 67 min; water concentration, 48%; and extraction temperature, 50 °C. The best predicted comprehensive evaluation value was 4.2634, and the mean value obtained from the experiment was 3.8678. Compared with traditional solvents, the antioxidant activity of NADES-7 extract was better, and the mean value of DPPH, FRAP (ferric reducing antioxidant power), and ABTS assays were 0.1555, 0.1132, and 0.0894 mmol Trolox/g dry weight, respectively. Experimental results show that ACO-BPNN can better optimize the extraction process of many different polar compounds in plant combinations.
研究领域:
药学
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化工
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生物技术
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 3.9
Chang Li,Zixuan Li,Yan Sun
Laccase , a green biocatalyst , has been widely used in the field of biodegradation with the assistance of electron mediators. Herein, a novel strategy, polyethyleneimine (PEI)-induced biomimetic mineralization , was explored and applied to co-immobilize laccase and 2,2′-azino bis (3-ethylbenzothiazoline)-6-sulfonic acid (ABTS). With the PEI doping, calcium phosphate (CaP) could facilely mineralize within 20 min to form PEI@CaP; ABTS could be co-mineralized with PEI to form (PEI + ABTS)@CaP. Laccase, a negatively charged protein, was then immobilized via electrostatic adsorption onto the embedded PEI in the carriers. Both of the immobilized laccase preparations, denoted as Lac-PEI@CaP and Lac-(PEI + ABTS)@CaP, showed high activity recoveries (96.4 % and 88.0 %, respectively), and increased pH tolerance and storage stability as compared to free laccase. ABTS and laccase loading densities of Lac-(PEI + ABTS)@CaP reached 0.83 mmol g −1 and 100 mg g −1 , respectively. Lac-(PEI + ABTS)@CaP presented higher malachite green (MG) degradation capability than free laccase and Lac-PEI@CaP with free ABTS, possibly due to the proximity effect of the co-immobilized laccase and ABTS. Moreover, Lac-(PEI + ABTS)@CaP exhibited reusability and kept 83.2 % of MG degradation activity after three recycles and 37.9 % in the sixth. This work provided a new strategy for the co-immobilization of laccase and electron mediator, and demonstrated the potential of the biocatalyst for organic pollutant removal.
研究领域:
生物技术
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环境科学
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生物化学
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材料科学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
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IF 4.3
Yifan Cui,Qiulan Li,Dezhi Yang,Yaling Yang
Peroxidase (POD)-mimicking nanozymes have got great progress in the sensing field, but most nanozyme assaying systems are built with a single-signal output mode, which is vulnerable to the effect of different factors. Thus, establishment of a dual-signal output mode is necessary for acquiring dependable and durable performance. This work described an Fe doped noradrenaline-based carbon dots and Prussian blue (Fe,NA-CDs/PB) nanocomposite as a POD-like nanozyme and modified gold nanoparticles (AuNPs) for the colorimetric and surface-enhanced Raman scattering (SERS) dual-mode sensor of Pb(II) in traditional Chinese medicine samples. With 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 3,3′,5,5′-tetramethylbenzidine (TMB) as the substrates, it was found that the addition of Pb(II) inhibited the POD-like activity of Fe,NA-CDs/PB and AuNPs, so it was used for colorimetric and SERS dual-mode assays. The POD-like activity was shown to be a “ping-pong” catalytic mechanism, whereas the addition of Pb(II) produced noncompetitive inhibition with modulatory effects on Fe,NA-CDs/PB. The linear response range for colorimetric and SERS sensor detection of Pb(II) was 0.01–1.00 mg/L with the detection limit of 5 μg/L and 8 μg/L, respectively. This dual-mode detection system shows excellent selectivity. More importantly, the Pb(II) in traditional Chinese medicine samples have successfully assayed with good recovery from 90.4 to 108.9 %.
研究领域:
分析化学
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纳米技术
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环境科学
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传统中药
✓ 已验证
DOI
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Ke Guo,Peng Zhao,Xinxin Yang,Jin Wang,Wei Chang,Simei Liu,Siqi Zhang,Tingting Zhang,Hao Yan,Yafeng Yan,Jie Wang,Xiping Chen,Churong Yu,Guoquan Wang
The present study reports the synthesis of a selenium nanocomplex (Se-TFPs) using purified polysaccharides from Tussilago farfara L (coltsfoot). It evaluates its structural characteristics, physicochemical properties, and inhibitory effects of Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. The influence of processing conditions on nanoparticle size and stability at 25 °C was assessed using particle size and zeta potential measurements. The Se-TFPs were synthesized by optimizing the processing conditions via response surface methodology, yielding nanoparticles with a selenium (Se)-to-polysaccharide mass ratio of 1:13.5, a Se-to-ascorbic acid molar ratio of 1:4.5, a selenite concentration of 10.7 mM, and a reaction time of 4.4 h. The resulting Se-TFPs had an average particle size of 107.2 nm and a zeta potential of -35.1 mV. Structural and physicochemical analyses confirmed successful nanoparticle formation. Compared to TFPs, Se-TFPs exhibited significantly enhanced scavenging activity against 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl radicals, and superoxide anion radicals. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, flow cytometry, and cell cycle apoptosis analysis revealed that Se-TFPs effectively inhibited MCF-7 cell proliferation at the S phase, with an IC50 value of 119.62 μg/mL.
研究领域:
纳米技术
·
药理学
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肿瘤学
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生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）