MOLECULAR ECOLOGY
·
2024
·
IF 4.5
Juliane P. C. Monteiro,Heike Pröhl,Mariana L. Lyra,Andrés E. Brunetti,Eli C. de Nardin,Thais H. Condez,Célio F. B. Haddad,Ariel Rodríguez
Colour signals play pivotal roles in different communication systems, and the evolution of these characters has been associated with behavioural ecology, integumentary production processes and perceptual mechanisms of the species involved. Here, we present the first insight into the molecular and histological basis of skin colour polymorphism within a miniaturized species of pumpkin toadlet, potentially representing the lowest size threshold for colour polytypism in tetrapods. Brachycephalus actaeus exhibits a coloration ranging from cryptic green to conspicuous orange skin, and our findings suggest that colour morphs differ in their capability to be detected by potential predators. We also found that the distribution and abundance of chromatophores are variable in the different colour morphs. The expression pattern of coloration related genes was predominantly associated with melanin synthesis (including dct , edn1 , mlana , oca2 , pmel , slc24a5 , tyrp1 and wnt9a ). Up-regulation of melanin genes in grey, green and brown skin was associated with higher melanophore abundance than in orange skin, where xanthophores predominate. Our findings provide a significant foundation for comparing and understanding the diverse pathways that contribute to the evolution of pigment production in the skin of amphibians.
研究领域:
进化生物学
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分子生物学
·
动物行为学
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两栖动物学
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DOI
PubMed
高影响因子
FOOD SCIENCE AND BIOTECHNOLOGY
·
2016
·Vol. 25
·期 2
·631-636
·
IF 0.699
Huang Xiaojun,Nie Shaoping,Yang Meiyan,Xie Jianhua,Li Chang,Xie Mingyong
Nine different edible oil types from 7 cities in China were analyzed. Trans fatty acid (TFA) contents in edible oils were determined, TFA distribution characteristics were analyzed, and a risk assessment was performed. Total TFA contents varied from 0.075±0.011 to 2.243±0.770 g/100 g of oil, with a mean content of 0.66 g/100 g of oil. TFA contents differed among different oil types and among different cities. Generally speaking, oil samples from northern areas of China contained more TFA, and lard usually had higher levels of TFA than other oils. An exposure assessment showed that the estimated daily intake of TFA from Chinese edible oils was low, and a risk assessment indicated that there was a low risk for TFA consumption in Chinese edible oils.
研究领域:
营养学
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食品安全
·
公共卫生
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Tianze Zhang,Rui Wang,Junpeng Xiao,Lu Li,Xinzhi Ma,Weiguang Zhang
Benefitting from the large interlayer spacing, ultrahigh conductivity and abundant surface chemistry, Ti 3 C 2 T x has been a promising electrode material for supercapacitors (SCs). CoS has attracted much attention due to its low cost, weak Co-S bond and relatively high theoretical capacity. Herein, CoS nanowires were grown on few-layered Ti 3 C 2 T x by one-step solvothermal method as a SC electrode. Within the composite, Ti 3 C 2 T x could function as conductive network and buffer matrix to provide ultra-fast electronic transport and relieve volume expansion of CoS nanowires. Simultaneously, the active CoS nanowires with high capacitance act as interlayer spacer to restrain the restacking of Ti 3 C 2 T x nanosheets . As a result, CoS/Ti 3 C 2 T x -5 electrode exhibits a remarkable improvement specific capacitance of 528 F g −1 at a current density of 1 A g −1 and ultrahigh capacitance retention of 99.3% after 20 000 cycles at a current density of 10 A g −1 . The attempts and efforts made in this work provide a prototype for achieving excellent electrochemical properties .
研究领域:
材料科学
·
能源存储
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL ECOLOGY
·
2026
·Vol. 52
·期 2
·27
·
IF 2.1
Peng Lishu,Huang Qianqian,Xiao Wei
Phytophagous mites are known to possess olfactory sensitivity, enabling them to detect plant volatiles. Unlike insects, which detect plant volatiles primarily via odorant receptors (ORs), Tetranychus cinnabarinus , a highly destructive phytophagous species, lacks ORs and ionotropic receptors (IRs); it may rely on its retained ionotropic co-receptor (IR25a-like) genes for olfactory detection. Whether these IR25a-like receptor mediate plant volatile perception in this species remains unknown. Here, we cloned three IR25a-like genes— TcinIR25a-1 , TcinIR25a-2 , and TcinIR25a-3 —and found that their expression was modulated by exposure to plant volatiles. RNA interference (RNAi) of TcinIR25a-1 or TcinIR25a-2 significantly altered female behavioral responses in Y-tube olfactometer assays, indicating their involvement in detecting plant volatiles. This study demonstrates for the first time that IR25a-like genes mediate plant volatile detection in T. cinnabarinus , offering new insights into mite olfactory mechanisms and potential molecular targets for behavior-based pest management.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Changhong Li,Taotao Dai,Jun Chen,Xin Li,Ti Li,Chengmei Liu,David Julian McClements
The solution turbidity and intrinsic fluorescence quenching increased after procyanidin was mixed with lactoferrin. The addition of procyanidin also caused a reduction in the surface hydrophobicity of the lactoferrin, suggesting procyanidin bound to non-polar patches on lactoferrin’s surfaces. Moreover, the binding interaction caused an appreciable alteration in the structure of both the polyphenol and protein. Thermodynamic analysis indicated the interaction was spontaneous and mainly driven by entropy changes, suggesting that hydrophobic interactions dominated. A computational docking simulation provided insights into the location of the most-likely binding sites on the protein, as well as the nature of the interaction forces involved. In particular, both hydrophobic and hydrogen bonding were found to be important. The binding of the procyanidin to the lactoferrin enhanced its foaming properties. These results may lead to the development of a new class of natural functional ingredients that can be used in food products to improve their quality attributes.
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
·
2021
·
IF 3.636
Yijing Han,Qianqian Zhang,Lizhu Chen,Dinglong Yang,Jianmin Zhao
The formation of extracellular traps (ETs) is an important innate immune mechanism that serves to combat different invading pathogens. In this study, zymosan significantly induced the formation of ETs in the hemocytes of Ruditapes philippinarum , and this effect was accompanied by translocation of the mitochondria to the cell surface. Zymosan stimulation clearly induced an increase in intracellular ROS and MPO production and an overexpression of ROS-related genes ( PI3K , AKT and HIF ). In response to the ROS burst, the mitochondrial membrane potential decreased, and the mitochondrial permeability transition pore opened. Conversely, mitochondrial superoxide inhibitor (Mito-TEMPO) significantly inhibited the formation of ETs, suggesting that mitochondrial ROS were necessary for the formation of ETs. In addition, we found that zymosan-induced ETs showed antibacterial activities against gram-negative and gram-positive bacteria, such as Vibrio anguillarum , Vibrio harveyi , Escherichia coli and Micrococcus luteus . Taken together, these findings elucidated a new antibacterial approach for R. philippinarum and highlighted the role of mitochondria in the formation of zymosan-induced ETs.
研究领域:
免疫学
·
海洋生物学
·
细胞生物学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Kai Tian,Jing Feng,Jiang Zhu,Jiagao Cheng,Mei Li,Xinghui Qiu
The cotton bollworm , Helicoverpa armigera , is a polyphagous pest threatening many economically important crops worldwide. Until recently, synthetic pyrethroids remain in wide use for controlling pest insects including the cotton bollworm. Understanding the metabolic mechanism of pyrethroids in a given pest can provide significant implication for a smart choice of insecticides, and such information is useful for the development of novel selective and safe insecticides. In this study, we used complexes of recombinant H. armigera cytochrome P450 CYP9A and NADPH-dependent cytochrome P450 reductase to investigate the capacity of three CYP9A paralogs in the transformation of seven structurally different pyrethroids by metabolism assays. The results showed that the three paralogous CYP9As were able to metabolize multiple pyrethroids. Interestingly, all the three CYP9As transformed pyrethrin-resembling pyrethroids (e.g. bioallethrin) more efficiently than the heavily modified ones (e.g. bifenthrin). These findings suggest that herbivorous insects can cope with synthetic insecticides using their physiological systems that initially evolved to survive exposure to the defensive chemicals in their host plants, adding support to the pre-adaptation hypothesis.
研究领域:
昆虫学
·
农药科学
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DOI
PubMed
高影响因子
Small
·
2022
·Vol. 18
·期 35
·2201949
·
IF 15.153
Xiaoning Li,Meng Li,Quanyu Shi,Hongmin Guo,Lidong Wang,Xiaolin Guo,Zhijun Chen,Jonathan L. Sessler,Huining Xiao,Tony D. James
Heavy metal pollution has resulted in numerous environmental challenges. However, classic approaches, involving the use of solid adsorbents are subject to limitations, including the high energy consumption required for processing before and after use. Accordingly, strategies that facilitate the use of metal capture media that extends beyond waste remediation are attractive. Herein, a porous fluorescent aerogel (CPC aerogel) is constructed by immersing amino-based carbon dots (CDs-NH 2 ) into a polyethyleneimine (PEI)/carboxymethylated cellulose (CMC) aerogel network for the simultaneous detection and adsorption of Cr(VI). Adsorption experiments confirm that the CMC/PEI containing CDs-NH 2 aerogel (CPC aerogel) exhibits good Cr(VI) extraction capacity, and can reach a level that conforms with industrial water safety standards. In addition, the CPC aerogel can continuously detect and remove Cr(VI) at high flux. Following Cr(VI) absorption, the CPC aerogel may be vulcanized (MS x -CPC gel) and used for solar thermoelectric generation resulting in power generation. Additionally, the MS x -CPC gel can be used for solar steam generation and exhibits excellent evaporation rates of ≈1.31 kg m –2 h –1 under one sun irradiation. The results serve to underscore how materials designed for metal ion recognition and adsorption once exhausted can be exploited to provide materials for solar thermoelectric power generation and seawater desalination.
研究领域:
环境科学
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材料科学
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能源科学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2022
·
IF 7.129
Tianxiu Qiu,Huan Wang,Lei Liu,Jiong Chen
Azoxystrobin (AZ) is one of the most widely used strobilurin fungicides in the world, and its residue has seriously endangered aquatic ecological security. Our previous data showed that AZ exposure may reduce the resistance of fish to rhabdovirus infection in aquatic environment. Here, we further reported a potential long-term adverse effect of AZ exposure on the antiviral and immunosuppressive recovery in fish, and observed that mitochondrial dynamic balance was disturbed by AZ in which excessive mitochondrial fission occurred in response to decreased ATP levels. When a recovery operation was performed in AZ-exposed cells and fish, infectivity of our model virus, spring viraemia of carp virus (SVCV), was significantly decreased in vitro (using the epithelioma papulosum cyprini [EPC] fish cell line) and in vivo (using zebrafish) in a time-dependent manner. Also, the expression of eight innate antiviral immune genes ( IFNs , ISG15 , MX1 , RIG-I , IRF3 , Nrf2 and HO-1 ) showed a similar change to SVCV replication between the longer exposure period and the expression recovery. Additionally, AZ facilitated horizontal transmission of SVCV in a static cohabitation challenge model, predicting the increase of the potential for the viral outbreak. Therefore, our data suggest that long-term effect of AZ on irreparable impairment in fish made AZ residue potentially greater for ecological risks.
研究领域:
水生毒理学
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免疫学
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病毒学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Brain Sciences
·
2023
·Vol. 13
·期 2
·200
·
IF 3.3
Li Wang,Zheng Gao,Gang Chen,Deqin Geng,Dianshuai Gao
Sleep disturbances are the most prevalent non-motor symptoms in the preclinical stage of Parkinson’s disease (PD). Adenosine, glial-derived neurotrophic factor (GDNF), and associated neurotransmitters are crucial in the control of sleep arousal. This study aimed to detect the serum levels of adenosine, GDNF, and associated neurotransmitters and explored their correlations with PD with sleep disorders. Demographic characteristics and clinical information of PD patients and healthy participants were assessed. Serum concentrations of adenosine, GDNF, and related neurotransmitters were detected by ELISA and LC-MS. The correlation between serum levels of adenosine, GDNF, and associated neurotransmitters and sleep disorders was explored using logistic regression. PD patients with sleep disorders had higher scores of HAMA, HAMD, ESS, UPDRS-III, and H-Y stage. Lower levels of adenosine, GDNF, and γ-GABA were observed in PD patients who had sleep problems. Logistic regression analysis showed adenosine and GDNF were protective factors for preventing sleep disorders. Adenosine combined with GDNF had a higher diagnostic efficiency in predicting PD with sleep disorders by ROC analysis. This study revealed low adenosine and GDNF levels may be risk factors for sleep disorders in PD. The decrease of serum adenosine and GDNF levels may contribute to the diagnosis of PD with sleep disturbances.Keywords:Parkinson’s disease;sleep disorders;adenosine;GDNF;neurotransmitters
研究领域:
神经科学
·
医学
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DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2020
·
IF 6.205
Xiaojia Shi,Tao Zhang,Hongxiang Lou,Huina Song,Changhao Li,Peihong Fan
Mitochondrion is a favorable therapeutic target in cancer, given its regulation of bioenergetics and cell death. Honokiol exhibits antiproliferative effects through mitochondria-mediated death signaling. To enhance its anticancer potential and selectivity, we conjugated honokiol to berberine, a mitochondria-targeting carrier. All designed derivatives displayed 1 order of magnitude increased cytotoxicity compared with the parent compounds, especially with massive cytoplasmic vacuoles. Biological evaluation demonstrated the representative compound 6b localized within the mitochondria, and mitochondrial dilation resulted in vacuolization. 6b induced vacuolation-associated cell death and apoptosis with obvious mitochondrial dysfunction, as demonstrated by booming reactive oxygen species generation, opening mitochondrial permeability transition pore, and reducing mitochondrial membrane potential. The targeting property also conferred 6b with selectivity for tumor cells compared to normal cells. 6b inhibited cancer cell proliferation in the zebrafish xenograft model. These results demonstrate that berberine-linked honokiol derivatives open up a direction for novel mitochondrial-targeting antitumor agents.
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PubMed
高影响因子
TOXICOLOGY
·
2016
·
IF 3.817
Xu Wang,Yijie Bai,Guyue Cheng,Awais Ihsan,Feng Zhu,Yulian Wang,Yanfei Tao,Dongmei Chen,Menghong Dai,Zhengli Liu,Zonghui Yuan
Quinoxaline 1,4-dioxides (QdNOs) are widely used as a kind of antibacterial growth promoter in animal husbandry. The adrenal cortex was found to be one of the main toxic targets of QdNOs, accompanied by a decreased aldosterone level. However, the way in which QdNOs decrease production of the hormone aldosterone is far from clear. To illustrate the mechanism by which QdNOs damage the adrenal cortex and decrease aldosterone hormone levels, the QdNOs were screened to choose the drug with most toxic effects on aldosterone production, and then to reveal the mechanism between the gene and protein profiles in human adrenocortical cells (NCI-H295R cells). The results found that quinocetone (QCT) showed the highest adrenal toxic effect among QdNOs. After exposing H295R cells to 10 and 20 μM QCT for 24 h, compared with blank cells, the gene and protein expression profiles obtained were analyzed by microarray and MALDI TOF/TOF mass spectrometry, respectively. The results of microarray analysis suggested that ABCG1 and SREBF1, which were involved in the cholesterol biosynthetic and metabolic processes, and CYP17A1, NR4A2 and G6PD, which were related to aldosterone biosynthesis, were important molecular targets. It has been speculated that PKC and ERK pathways might be involved in the reduction of aldosterone production caused by QCT, through enhanced mRNA expression of CYP17A1. Additionally, JNK and p38MAPK signal transduction pathways might participate in apoptosis induced by QCT. Twenty-nine and 32 protein spots were successfully identified when cells were treated with 10 and 20 μM QCT, respectively. These identified proteins mainly included material synthesis and energy metabolism-related proteins, transcription/translation processing-related proteins, signal transduction proteins, cytoskeletal proteins, molecular chaperones, proteins related to response to stress, and transport proteins. Further investigations suggested that oxidative stress caused by QCT was exacerbated through disruption of the Keap1/Nrf2/ARE anti-oxidative stress pathway. Taken together, the data demonstrated for the first time that the Keap1/Nrf2/ARE pathway plays a crucial role in adrenal toxicity, and that CYP17A1 was the key switch to reduce the aldosterone production induced by QCT. Furthermore, large numbers of genes and proteins and entry points for research in the inhibition of aldosterone synthesis induced by QCT were offered, which will provide new insight into the adrenal toxicity of QdNOs and help to provide a theoretical foundation for the formulation of safety controls for products obtained from animals and to design new QdNOs with less harmful effects.
研究领域:
毒理学
·
药理学
·
内分泌学
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分子生物学
·
细胞生物学
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DOI
PubMed
高影响因子
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