Artificial Cells Nanomedicine and Biotechnology
·
2017
·
IF 5.605
Wenping Wang,Yaya Lei,Hong Sui,Wenping Zhang,Rongyue Zhu,Jun Feng,Hong Wang
The aim of the present study was to prepare and evaluate microparticle formulation encapsulated with glycyrrhetinic acid (GA) based on bovine serum albumin (BSA). The drug-loaded nanoparticles were firstly formed by a simple desolvation method, and were further assembled into microparticles using zinc chloride and glutaraldehyde as crosslinkers. The obtained microparticles contained approximately 30% (w/w) drug and showed as spherical particles with a size of about 2 μm. Differential scanning calorimetry (DSC) and X-ray powder diffraction (XRPD) analysis indicated that GA lost its crystallinity during the nano/microencapsulation process. In vitro dissolution study demonstrated a typical sustained-release pattern for 24 h with a burst of 28.1% at the first 30 min, which fitted well by Higuchi model. After intravenous administration into mice, the microparticle formulation remained a higher drug level than the solution formulation in blood and liver for more than 18 h. These results suggested the potential benefit of using the prepared albumin microparticles as a promising vector for enhanced liver delivery of poorly water-soluble drug.
研究领域:
药剂学
·
生物医学工程
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Changyan Dong,Yi Cheng,Meihui Zhang,Mianhua Chen,Zhongli Yan,Sumei Zhou,BenxuYang,Qingbin Guo,Changlu Wang,Shufen Wu
In this study, four Monascus pigments (ankaflavin, AK; monascin MS; rubropunctatin, O 1 ; monascorubrin, O 2 ) were proved to exhibit considerable anti-glycation properties in bovine serum albumin (BSA)-fructose model. AK (40.62 %) and MS (48.38 %) were found to exert lower inhibitory effects on the formation of fluorescent advanced glycation end products (AGEs) than aminoguanidine (59.4 %), while O 1 (90.64 %) and O 2 (93.82 %) displayed much stronger abilities. AK and MS could trap methylglyoxal (MGO) with maximum capture rates of 85.67 % and 84.90 %, respectively, and only mono-MGO adducts of them were detected. LC-Orbitrap MS/MS analysis revealed that four pigments significantly altered the type and reduced the number of the glycated sites and they all covalently bound to BSA, with O 1 and O 2 possessing high reactivity. Altogether, AK and MS suppressed fluorescent AGEs formation mainly via trapping MGO and covalently interacting with BSA, and blocking free amino groups was the dominant mechanism for O 1 and O 2 . These findings presented new insights into Monascus pigments as dietary supplement for inhibiting protein glycation.
研究领域:
生物化学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Siying Che,Gaoqiong Deng,Tingyong Ling,Haiyan Fu,Yuanbin She
Monosultap (Mon) poses significant environmental and human health risks due to its widespread and irregular use. However, due to the lack of intrinsic fluorophores and identifiable sites in monosultap, there were few fluorescence analytical methods achieved sensitive detection of monosultap. Hence, we present a BSA-Cu nanocluster-based turn-off-on fluorescent sensor for sensitive detection of monosultap. The sensor exhibits fluorescence quenching ("turn-off") in the presence of DTAB-CuTPyP. The competitive binding of monosultap with DTAB-CuTPyP switches the sensor to the "turn-on" state. Under optimized conditions, the sensor demonstrates a linear response to monosultap concentrations ranging from 0.33 × 10⁻ 2 to 3.33 × 10⁻ 2 mg/kg, with a detection limit of 0.098 mg/kg, which is below the permissible exposure limit (approximately 1 mg/kg). In addition, the co-existence of nine interference pesticides does not affect the sensor's ability to detect monosultap. This fluorescence restoration mechanism enables sensitive monosultap detection and provides a guide for detecting trace pesticides in food.
研究领域:
分析化学
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环境科学
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食品安全
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产品关联
DOI
高影响因子
Theranostics
·
2026
·Vol. 16
·期 9
·4521
·
IF 14.9
Zhe Liu,Qinzhou Zheng,Dong Zhou,Anqi Lin,Lan Xiao,Haifeng Liu,Keqin Ji,Huifen Qiang,Xinxin Sui,Yulin Li,Yan Wu,Jie Gao,Lan Liao,Xiaohuan Yuan
Background The process of healing wounds in diabetic patients is intricate and is notably obstructed by a disordered wound microenvironment, characterized by chronic inflammation and elevated blood glucose. The combination of stem cell therapy and drug treatment is seen as a promising application in future. However, the limited proliferative capacity of stem cells and inadequate drug availability present significant challenges for achieving optimal therapeutic outcomes. Methods In this study, open porous poly (lactic‒coglycolic acid) (PLGA) microspheres were designed and synthesized via gas-assisted volatilization microemulsion technology. These microspheres encapsulate curcumin and allow its slow release, thereby enhancing wound repair. The large pores in the microspheres provide ample support for bone marrow stem cells (BMSCs), enabling continuous drug release over a period of 35 days. Results The sustained release of curcumin promoted stem cell proliferation and maintained stem cell activity. Additionally, it facilitates remodeling of the wound immune microenvironment. Additionally, the microspheres can activate mitochondrial autophagy in cells, effectively alleviating wound inflammation. Conclusions The combined actions of curcumin and stem cells aid in regenerating blood vessels and revitalizing the collagen network where the injury occurred, thus improving wound healing capabilities. Consequently, integrating drugs with stem cells and microspheres holds significant potential for diabetic wound treatment.
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Yong Zhu,Cuifeng Jiang,Jinjiang Yao,Xinmeng Wu
Resolving the aggregation of osmium (Os) nanozyme is important for improving its catalytic activity. Herein, Os nanoparticles-Bovine serum albumin-MnO 2 composite nanosheets (OsNPs-BSA-MnO 2 ) were synthesized by loading Os NPs on BSA-MnO 2 nanosheets. The Os NPs well dispersed on the surface of the nanosheets. The composite exhibited specific peroxidase-like activity with negligible oxidase-like activity. The catalytic mechanism was proved due to the generation of ·OH. Steady-state kinetics revealed stronger affinity for both H 2 O 2 and 3,3′,5,5′-tetramethylbenzidine (TMB) substrate. Interestingly, OsNPs-BSA-MnO 2 composite nanosheets exhibited a much lower activation energy than other osmium-based nanozymes, indicating superior catalytic performance. Moreover, ascorbic acid could effectively inhibit the catalytic activity of composite nanosheet. Thus, a colorimetric method for ascorbic acid detection was established with the limit of detection (LOD) of 1.69 μM and excellent selectivity. For practical application, ascorbic acid in Water-soluble C100 and fetal bovine serum was detected with satisfactory recoveries
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产品关联
DOI
高影响因子
Theranostics
·
2026
·Vol. 16
·期 14
·8263
·
IF 14.9
Jingyi Yang,Qi Li,Pi Zhao,Zixin Luo,Zhaokai Wang,Rui Yang,Min Zhou,Jie Zhou,Daozhen Chen,Yu Chen
Rationale Since the therapeutic resistance of triple-negative breast cancer (TNBC) is mainly attributable to excessive glutathione (GSH) accumulation and its ‘cold’ immune landscape, we designed biomimetic CuZnS@BSA nanoregulators that exploit a pH-triggered ‘disarm-and-attack’ cascade, thereby initiating a well-defined, sequential therapeutic process in the acidic tumor microenvironment. Methods Biomimetic CuZnS@BSA nanoclusters were synthesized via a self-assembly method. Their pH-responsive release kinetics and synergistic therapeutic mechanisms (GSH depletion, ROS generation, and cuproptosis) were systematically evaluated in vitro using 4T1 cells. In vivo anti-tumor efficacy, immune microenvironment remodeling, and anti-metastatic effects were investigated in subcutaneous and lung metastasis TNBC mouse models, both alone and in combination with PD-L1 blockade. Results The platform first releases H 2 S to deplete intracellular GSH, thus removing the major antioxidant defenses of the tumor, then follows with the release of Cu 2+ to induce cuproptosis, which effectively bypasses the apoptosis resistance commonly seen in TNBC. In addition, the released Zn 2+ acts as an immune modulator by promoting the recognition of leaked mitochondrial DNA. This activates the cGAS-STING signaling pathway, and in vivo experiments clearly showed that it remodels the tumor microenvironment in a highly favorable manner, characterized by increased CD8 + T cell infiltration and enhanced dendritic cell maturation. Conclusion Combining this nanoregulator with PD-L1 blockade led to potent suppression of both subcutaneous tumor growth and lung metastasis, thus providing a direct, elegant link between metabolic reprogramming and systemic immune activation for TNBC therapy.
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Huirong Tan,Mingrun Tang,Yufeng Che,Xuanao Li,Zhiwen Tu,Xinghong Zhao,Hongping Wan
In mucosal immunity, targeting microfolds (M)cells of the mucosal epithelium, in combination with suitable adjuvants, is critical for eliciting robust immune response. In the present study, we devised a virus like nanoparticles (VLP)-based nano-delivery platform functionalized with the M cell-targeting peptide iRGD (RGDKGPDC) to enhance the immune responses following intranasal administration. Bovine serum albumin (BSA) was selected as a model antigen, and both BSA and iRGD were conjugated to the VLP surface, forming VLP-BSA-iRGD nanoparticles. Leveraging its M cell-targeting capability, VLP-BSA-iRGD demonstrated enhanced uptake by dendritic cells (DCs) and improved trafficking to lymph nodes compared to BSA alone in a mouse model. VLP-BSA-iRGD enhanced the CCL20 secretion and stimulated the DC activation, as evidenced by an in vitro cell co-culture model. Additionally, it enhanced antigen permeability by disrupting the distribution of the ZO-1 protein in epithelial cells. Importantly, VLP-BSA-iRGD induced stronger humoral and cellular immune responses, accompanied by the upregulation of TLR2/4 expression and activation of the MYD88/NF-κB signaling pathway in DCs. While BSA served as a proof-of-concept antigen, this platform may be broadly applicable to other protein-based subunit vaccines requiring intranasal administration.
研究领域:
免疫学
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纳米医学
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疫苗学
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药物递送
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黏膜免疫学
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产品关联
DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2018
·Vol. 2
·期 1
·69-76
·
IF 0
Zhen Wen-Yao,Bai Jing,Jia Xiao-Dan,Wu Lie,Jiang Xiu-E
The local tumor hypoxia has become one of the main obstacles for photodynamic therapy (PDT).To break through this barrier, the fabrication of O 2 -evolving nanoplatform has been more significant. Herein, the uniform methylene blue (MB)–bovine serum albumin (BSA)–Fe(III) nanoparticles (MB–BSA–Fe(III) NPs) were successfully synthesized by biomineralization approach. MB–BSA–Fe(III) NPs show high loading capacity of MB, and Fe(ΙΙΙ) has excellent catalytic performance towards abundant H 2 O 2 in cancer cells to generate O 2 , overcoming tumor hypoxia. All these combination effects remarkably increase the therapeutic efficiency of PDT, resulting in almost complete destruction of cancer cells. Most importantly, MB–BSA–Fe(III) NPs exhibit T 2- magnetic resonance imaging, which have the potential for precise visualization of the tumor location. Our results demonstrate that MB–BSA–Fe(III) is a novel H 2 O 2 -responsive and O 2 -evolving nanoplatform and it has great potential for clinical applications such as cancer diagnosis, treatment or drug delivery.
研究领域:
纳米医学
·
肿瘤学
·
生物材料科学
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产品关联
DOI
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yan Jun Ju,Na Li,Shi Gang Liu,Lei Han,Na Xiao,Hong Qun Luo,Nian Bing Li
The ratiometric fluorescence measurement has drawn great attention due to its selective and sensitive sensing property for numerous analytes. In this sensing system, a ratiometric fluorescence method is described for the determination of malachite green (MG) based on bovine serum albumin-stabilized Au nanoclusters (BSA-AuNCs) with dual emission. The as-prepared BSA-AuNCs exhibit two emission peaks around 430 and 610 nm under a single excitation, which may be from the intrinsic structure of BSA and AuNCs, respectively. As MG was added, the fluorescence at 610 nm was obviously quenched, and the fluorescence intensity at 430 nm decreased slowly, which might be mainly ascribed to fluorescence resonance energy transfer and dynamic quenching. The fluorescence intensity ratio of F 430 / F 610 shows good linearity for detecting MG in the 0.3–20 μM range with a low detection limit of 0.19 μM. In addition, the ratiometric sensing strategy is successfully applied to real water samples, which reveals its potential practicability for detecting MG in environmental water samples.
研究领域:
分析化学
·
环境科学
·
纳米技术
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DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Junyang Zhang,Yacong Yang,Zechun Sun,Danyang Zhao,Yingxiu Gao,Tianhan Shen,Yan Li,Zongli Xie,Yuning Huo,Hexing Li
Membrane technologies offer great potential in desalination and wastewater treatment . However, the serious issue of membrane fouling results in the unsatisfactory sustainability and reusability for treating wastewater containing high level of organic foulants. In this work, a photocatalytic membrane reactor system combining Ag@BiOBr photocatalyst and polyvinylidene fluoride (PVDF) ultrafiltration with remarkable anti-fouling property was constructed. The integrated Ag@BiOBr/PVDF photocatalytic ultrafiltration membranes were prepared by blending Ag@BiOBr nanoparticles (NPs) into PVDF membrane via phase inversion method. The membrane separation performance and fouling resistance were studied in a cross-flow ultrafiltration system using bovine serum albumin (BSA) as the model foulants. Compared with the pristine PVDF membrane, the hydrophilicity and surface smoothness of composite membranes were promoted by photocatalyst-incorporation, the Ag@BiOBr/PVDF membrane with the optimal Ag@BiOBr blending content of 2.0 wt% achieved both enhanced water flux and higher BSA rejection efficiency. More importantly, the mechanism of anti-fouling and self-cleaning performances were further studied, the photocatalysis process based on Ag@BiOBr/PVDF membrane effectively decomposed the BSA foulant into broken protein with induced change of carbonyl, ionic bond and hydrogen bond contents in BSA foulants via the active radicals, weakened the intermolecular force of BSA foulants to prevent membrane pore blockage. Meanwhile, the broken protein peptide chains with more negative charges could result in the strength of electrostatic repulsive forces between BSA molecules and membrane. As a result, the BSA foulant could be stably removed in the Ag@BiOBr/PVDF photocatalytic ultrafiltration system under visible light irradiation, achieving the excellent anti-fouling performance by preventing the membrane pore blockage and foulant adhesion. We believe the Ag@BiOBr/PVDF photocatalytic membrane presented in this study will be one example among many photocatalyst-membrane combinations that can be further pursued for organic pollutants removal. In particular, we expect the theoretical support can present a great opportunity to design novel photocatalytic membranes with sustainable anti-fouling performance.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Yue-Na Sun,Lei Shi,Mei-Li Shi,Li-Qing Yu,Hong-Yuan Yan,Yun-Kai Lv
BSA functionalized hybrid monolithic column prepared by in-situ dynamic grafting on traditional stainless steel column for enantiomeric separation. A hybrid silica monolithic column (HMC) was prepared using a single precursor (methytrimethoxysilane) in stainless steel HPLC column (50 mm × 4.6 mm) by sol–gel method. The chiral hybrid monolithic column (CHMC) was prepared by grafting 3-aminopropyl trimethoxysilane onto HMC, and then reacting bovine serum albumin (BSA) with Glutaraldehyde. The preparation conditions were optimized, and the maximum grafting amount of BSA was 48.6 mg g −1 . The CHMC was characterized and its chromatographic performance was tested. The results showed that the CHMC has a homogeneous network skeleton structure, good stability and reproducibility. The CHMC was combined with HPLC, under optimized chromatographic conditions, the baseline separation of tryptophan and pidotimod enantiomers were successfully achieved.
研究领域:
分析化学
·
生物化学
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Jun Wang,Ling Cai,Hao Wang,Liuzhu Zhou,Xinyi Zhu,Minghui Ji,Huijun Jiang,Daorong Wang,Yuan Wu,Jin Chen
To fulfill diverse application-specific purposes, functional nanoparticles encapsulated into protein-based self-assemblies show promising advantages includes easy preparation, high biocompatibility and multiple properties. In this study, the model protein of BSA used for multi-component self-assembly, including hydrophobic Fe 3 O 4 nanoparticles, nano-ZnO and curcumin, was constructed through hydrophobic interactions. The obtained composites of BSA-Fe 3 O 4 &ZnO and BSA-Fe 3 O 4 &ZnO&curcumin self-assemblies were tested for antibacterial and drug delivery, respectively. It was found that the multicomponents loaded into protein assembly confer a simple yet robust synthetic method resulting in the produced nanocomposites with high stability and desirable biocompatibility. Notably, the protein self-assemblies successfully increased the antibacterial efficacy of hydrophobic ZnO nanoparticles, and promote the intracellular delivery of poorly soluble curcumin to exert significant killing effects against cancer cells. Therefore, the constructed multi-component contained protein self-assembly that enables hydrophobic interactions of compositional nanoparticles holds promising potentials for the versatile uses in biomedical aspects.
研究领域:
生物医学工程
·
材料科学
·
药学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）