Acta Biomaterialia
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2017
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IF 6.319
Yayun Peng,Dongzhi Yang,Weifei Lu,Xiongwei Hu,Hao Hong,Ting Cai
Various carbonaceous nanomaterials , including fullerene , carbon nanotube , graphene, and carbon dots , have attracted increasing attention during past decades for their potential applications in biological imaging and therapy. In this study, we have developed a fullerene-based tumor-targeted positron emission tomography (PET) imaging probe. Water-soluble functionalized C 60 conjugates were radio-labeled with 64 Cu and modified with cyclo (Arg-Gly-Asp) peptides (cRGD) for targeting of integrin α v β 3 in glioblastoma. The specificity of fluorescein-labeled C 60 conjugates against cellular integrin α v β 3 was evaluated in U87 MG (integrin α v β 3 positive) and MCF-7 cells (integrin α v β 3 negative) by confocal fluorescence microscopy and flow cytometry. Our results indicated that cRGD-conjugated C 60 derivatives showed better cellular internalization compared with C 60 derivatives without the cRGD attachment. Moreover, an interesting finding on intra-nuclei transportation of cRGD-conjugated C 60 derivatives was observed in U87 MG cells. In vivo serial PET studies showed preferential accumulation of cRGD-conjugated C 60 derivatives at in U87 MG tumors. In addition, the pharmacokinetic profiles of these fullerene-based nanoparticles conjugated with cRGD and 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) fit well with the three compartment model. The renal clearance of C 60 -based nanoparticles is remarkably fast, which makes this material very promising for safer cancer theranostic applications. Statement of significance Safety is one of the major concerns for nanomedicine and nanomaterials with fast clearance profile are highly desirable. Fullerene is a distinct type of zero-dimensional carbon nanomaterial with ultrasmall size, uniform dispersity , and versatile reactivity. Here we have developed a fullerene-based tumor-targeted positron emission tomography imaging probe using water-soluble functionalized C 60 conjugates radio-labeled with 64 Cu and modified with cyclo (Arg-Gly-Asp) peptides (cRGD) for glioblastoma targeting. The improved tumor targeting property along with fast renal clearance behavior of C 60 -based nanoparticles makes this material very promising for future safer cancer theranostic applications.
研究领域:
纳米医学
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生物医学工程
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分子影像
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癌症治疗
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放射学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Xiaoying Kong,Haoyu Chen,Fuqiang Li,Fenglan Zhang,Yuping Jiang,Junyao Song,Yuanliang Sun,Bin Zhao,Jinsheng Shi
Application of Combined photodynamic therapy (PDT) and photothermal therapy (PTT) has become one of the most promising strategy to replace antibiotics and avoid the epidemic of drug-resistant strains during wound healing. However, high amount of reactive oxygen species (ROS) and high temperature cause severe stress response to normal tissues, leading to potential risks of wound healing. Herein, a three-dimension chitosan hydrogel melanin-glycine-C60 nanoparticles (MGC NPs) were prepared to realized effective anti-bacterial activity, immune activation and macrophage autophagy promotion in three-dimensional wound space without triggering stress response. MGC NP is a composite polymer material composed of natural melanin polymer, oligopeptide and carbon-based material, which showed excellent biological safety. By regulating the peptide length between melanin and C60 and nanoparticle content, a high ROS/heat environment at the upper wound site and a low ROS/heat environment at the lower region adjacent to the wound tissue were established to obtain a three-dimension hydrogel with precise PDT and PTT efficiency in different regions. Highly effective PDT/PTT was used to kill microorganisms in upper region, thus providing a barrier to reduce microbial infection. Mild PDT/PTT in lower region promoted the polarization of M1 macrophage to M2 macrophage and activated autophagy of M2 macrophages, regulating the immune microenvironment and promoting wound repair. In conclusion, the novel three-dimensional PDT/PTT therapy based on natural macromolecules proposed in this study accelerates wound healing through dual pathways on the premise of avoiding wound stress response, which is of great significance for the development of clinical strategies for phototherapy.
研究领域:
材料科学
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生物医学工程
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纳米技术
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伤口愈合
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免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2016
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IF 4.758
Han Zhang,Qiyi Lu,Fumei Zuo,Ruo Yuan,Shihong Chen
A novel “signal-on” biosensor for the detection of concanavalin A (Con A) was designed utilizing an enzymatic reaction to in situ generate coreactant of poly(9,9-di- n -octylfluorenyl-2,7-diyl) (PFO). Firstly, PFO polymers were wrapped with graphene oxide nanosheets to obtain GO@PFO nanocomposite, which was modified onto the surface of the electrode as a matrix for immobilizing phenoxy-derivatized dextran (Dexp) through π-π stacking interaction. Then Con A interacted with Dexp through the specific carbohydrate-protein interaction. Finally, glucose oxidase (GOx) and lactate oxidase (LOx) decorated gold nanoparticles@C 60 -Polyamidoamine (Au@C 60 -PAMAM) was bound onto Con A via a biospecific interaction between Con A and the internal residues of GOx, thus achieving a sandwich structure. In this construction strategy, LOx catalyzed the oxidation of lactate to in situ generate H 2 O 2 , which could act as the coreactant of PFO to enhance the ECL intensity. Due to the excellent ECL property of PFO–H 2 O 2 system, our prepared biosensor showed a wide linear range from 0.001 ng/mL to 10 ng/mL, and a low detection limit down to 0.0003 ng/mL. The integration of excellent ECL property of PFO–H 2 O 2 system and excellent catalytic activity of Au@C 60 -PAMAM would open a new and promising platform for fabricating ECL biosensors and biocatalysts.
研究领域:
分析化学
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生物传感器
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电化学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
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2019
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IF 10.273
Yuhan Chen,Xinzhu Liu,Shuliang Guo,Jun Cao,Jing Zhou,Jianli Zuo,Lijuan Bai
The present work described a novel sandwich-type electrochemical aptasensor for rapid and sensitive determination of Mycobacterium tuberculosis MPT64 antigen. Herein, a novel carbon nanocomposite composed of fullerene nanoparticles , nitrogen-doped carbon nanotubes and graphene oxide (C 60 NPs-N-CNTs/GO) was facilely synthesized for the first time, which not only possessed a large specific surface area and excellent conductivity , but also exhibited outstanding inherent electroactive property, and therefore served as nanocarrier and redox nanoprobe simultaneously. Gold nanoparticles (AuNPs) was then uniformly anchored onto the surface of such nanocomposite via Au–N bonds to bind with MPT64 antigen aptamer Ⅱ (MAA Ⅱ), forming the tracer label to realize generation and amplification of electrochemical signal. Additionally, conductive polyethyleneimine (PEI)-functionalized Fe-based metal-organic framework (P-MOF) was used as a sensing platform to absorb bimetallic core-shell Au–Pt nanoparticles ( [email protected] ), which could accelerate electron transfer and increase the immobilization of MPT64 antigen aptamer Ⅰ (MAA Ⅰ). After the typical sandwich-type protein-aptamer recognition, the inherent electroactivity of the tracer label was provoked by tetraoctylammonium bromide (TOAB), leading to a well-defined current response. Under the optimum condition, the proposed aptasensor showed a wide linear range for MPT64 detection from 1 fg/mL to 1 ng/mL with a limit of detection (LOD) as low as 0.33 fg/mL. More importantly, it was successfully used for MPT64 antigen detection in human serum, exhibiting a promising prospect for TB diagnosis in clinical practice.
研究领域:
分析化学
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纳米技术
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生物医学工程
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电化学
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生物传感器
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医疗诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）