WATER AIR AND SOIL POLLUTION
·
2026
·Vol. 237
·期 9
·553
·
IF 3.5
Wang Bingyuan,Wang Fengwei,Lu Hongsheng
Microbial degradation has attracted more and more attention to be an effective way to effectively treat pesticide in the environment and save treatment costs. However, the specific degradation mechanism and the influence of microorganisms on the environment of some pesticides are still unclear. In this study, the Serratia marcescens BCD1 (PV133529) which isolated from the blueberry soil showed a 20 days degradation rate of 93.14% and 91.09% for 50 mg/L and 250 mg/L λ-cyhalothrin, respectively. Product analysis revealed the pathway of λ-cyhalothrin degradation by Serratia marcescens BCD1, including the removal of cyano groups and the cleavage of ester bonds. The morphological changes of BCD1 in the presence of different concentrations of λ-cyhalothrin were observed by SEM electron microscopy, and the cell wall of BCD1 was wrinkled and deformed, and the cell length was shortened under the stress of λ-cyhalothrin. This study indicates that BCD1 as a promising microorganism for λ-cyhalothrin residue and improve blueberry quality in a treatment setting. Graphical
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Theranostics
·
2026
·Vol. 16
·期 8
·4283
·
IF 13.3
Yangyang Qu,Yifeng Shang,Shixing Luo,Xiaomin Pei,Yuanming Xiao,Jinmin Zhao,Li Zheng,Chuanan Liao,Ruiming Liang
Rationale: Bone defects pose a persistent challenge in orthopedic medicine due to their limited self-repair capacity. Although guided bone regeneration scaffolds have shown therapeutic potential, their clinical efficacy remains constrained by their suboptimal osteoinductive capability. Methods: Herein, we developed biodegradable piezoelectric polyhydroxybutyrate-barium titanate (PHB-BT) nanofiber scaffolds capable of generating synergistic piezoelectric stimulation for bone repair when integrated with low-intensity pulsed ultrasound (LIPUS). Results: Compared with conventional PHB scaffolds, PHB-BT nanofiber scaffolds showed enhanced piezoelectric properties and excellent biocompatibility, thereby facilitating sustained osteogenic activity. In vitro studies revealed that these scaffolds significantly promoted the osteogenic differentiation of bone marrow mesenchymal stem cells under LIPUS stimulation. Notably, in vivo evaluations demonstrated that these scaffolds substantially accelerated bone defect repair, with complete scaffold degradation observed after eight weeks. Mechanistically, PHB-BT nanofibers improved osteogenesis via activating the Ca 2+ /calcineurin/nuclear factor of activated T-cells signaling pathway in response to ultrasound stimulation. Conclusions: These findings have significant implications for the design of next-generation, implantable electrical stimulators capable of providing sustained electromechanical cues for personalized bone tissue engineering applications.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Sibo Zhao,Huilin Wen,Yangteng Ou,Minhui Li,Lancheng Wang,Huimin Zhou,Bin Di,Ziyi Yu,Chi Hu
Biosensors integrating genetically programmed living cells into hostile and synthetic environments ( e.g. , devices or matrices) provide powerful tools for scientific research and enable new technological applications. However, it remains a challenge to maintain the viability and functionality of the living components exposed to different chemical, physiological or mechanical conditions in specific applications. To this end, we report herein a microfluidic approach to encapsulate microorganisms in gel-shell beads (GSBs) as a novel kind of biosensor. Inspired by biosilicification, hydrogel beads are coated with a mesoporous shell that can prevent cell leakage and afford mechanical stability, with the porous hydrogel structure naturally providing sustained supplies of water and nutrients to the embedded microorganisms. Communications between compartmentalized bacterial strains and the environment is achieved through the diffusion of signalling molecules across the mesoporous shell. Application of GSBs as living cell-based sensors is exemplified in a quorum sensing system, where genetically engineered Escherichia coli cells are encapsulated in GSBs and can respond to autoinducer concentrations ranging from 10 −6 to 10 −7 mol/L as secreted by human pathogen Pseudomonas aeruginosa in the infection site.
研究领域:
生物技术
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材料科学
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微生物学
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化学工程
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STEROIDS
·
2011
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IF 3.106
Long Ji,Yonglei Yuan,Liping Luo,Zhe Chen,Xiaoqiong Ma,Zhongjun Ma,Lin Cheng
Michael reaction acceptors (MRAs) are a class of active molecules that are directly or indirectly involved in various cellular processes, including the regulation of many signaling pathways . In this study, the inducible nitric oxide synthase (iNOS) assay was used to demonstrate that the dichloromethane extract of Physalis alkekengi var . franchetii (DCEP) possesses anti-inflammatory activity that might be attributed to the modification of key cysteine residues in IKKβ by the MRAs in DCEP. To isolate these MRAs, glutathione (GSH) was employed, and a simple ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) screening method was developed to investigate the GSH conjugates with potential MRAs. Five physalins, including one new compound isophysalin A ( 2 ), together with four known steroidal compounds, physalin A ( 1 ), physalin O ( 3 ), physalin L ( 4 ) and physalin G ( 5 ), were isolated to evaluate the GSH conjugating abilities, and it was indicated that compounds 1 , 2 and 3 , which had a common α,β-unsaturated ketone moiety, exhibited conjugating abilities with GSH and also showed significant nitric oxide (NO) production inhibiting activities. The anti-inflammatory activities of compounds 1 , 2 and 3 might be attributed to their targeting multiple cysteine residues on IKKβ; therefore, the alkylation of IKKβ by compound 1 was further studied by micrOTOF-MS. The result showed that six cysteine residues (C 59 , C 179 , C 299 , C 370 , C 412 , and C 618 ) were alkylated, which indicated that IKKβ is a potential target for the anti-inflammatory activity of physalin A.
研究领域:
药理学
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化学
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生物化学
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FISH & SHELLFISH IMMUNOLOGY
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2017
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IF 3.148
Lei Liu,Xiao Tu,Yu-Feng Shen,Wei-Chao Chen,Bin Zhu,Gao-Xue Wang
Different viruses could induced ROS generation to alter intracellular redox state in the host cells, and unbalanced redox state was suggested to have various effects on viral replication. In this study, we investigated the influence of reactive oxygen species (ROS) on replication of spring viraemia of carp virus (SVCV) in fish cells. After SVCV infection, there existed a time-dependent increase in ROS generation. The present results revealed that antioxidant N-acetyl- l -cysteine (NAC) resulted in a lower ROS levels and increased SVCV replication in EPC cell. In contrast, a GSH synthesis inhibitor buthionine sulfoximine (BSO) induced ROS generation and decreased SVCV replication. In addition, activation of NF-κB suppressed SVCV replication by using two inhibitors of cytokine-induced IκBα phosphorylation. More importantly, enhancement of the activity of NF-κB was found in BSO treatment, which indicated that dropped SVCV replication likely occurred via ROS activation of NF-κB. Overall, our results revealed that the SVCV infection and replication could generate ROS and be affected by the redox state, where this progression was associated with the alteration in NF-κB pathway induced by oxidative stress .
研究领域:
病毒学
·
细胞生物学
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PLoS One
·
2013
·Vol. 8
·期 9
·e74100
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IF 3.73
Heyu Chen,Yao Wang,Jixi Zhang,Yingxin Ma,Caixia Wang,Ying Zhou,Hongchen Gu,Weihai Ying
Aim
To test the hypothesis that NAD + -carrying mesoporous silica nanoparticles (M-MSNs@NAD+) can effectively deliver NAD + into cells to produce cytoprotective effects.
Methods & Materials
NAD + was incorporated into M-MSNs. Primary rat astrocyte cultures and PC12 cells were treated with H 2 O 2 , followed by post-treatment with M-MSNs@NAD+. After various durations of the post-treatment, intracellular NAD + levels, intracellular ATP levels and lactate dehydrogenase (LDH) release were determined.
Results & Discussion
M-MSNs can be effectively loaded with NAD + . The M-MSNs@NAD+ can significantly attenuate H 2 O 2 -induced NAD + and ATP decreases in both astrocyte cultures and PC12 cells. M-MSNs@NAD+ can also partially prevent the H 2 O 2 -induced LDH release from both astrocyte cultures and PC12 cells. In contrast, the NAD + that is spontaneously released from the M-MSNs@NAD+ is insufficient to prevent the H 2 O 2 -induced damage.
Conclusions
Our study has suggested the first approach that can effectively deliver NAD + into cells, which provides an important basis both for elucidating the roles of intracellular NAD + in biological functions and for therapeutic applications of NAD + . Our study has also provided the first direct evidence demonstrating a key role of NAD + depletion in oxidative stress-induced ATP decreases.
研究领域:
神经科学
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分子生物学
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纳米技术
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CHEMOSPHERE
·
2021
·
IF 7.086
Ting He,Jianguo Bao,Yifei Leng,Shuqiong Kong,Jiangkun Du,Xu Li
Doxycycline (DC) is a second generation tetracycline antibiotic and its occurrence in the aquatic environment due to the discharge of municipal and agricultural wastes has called for technologies to effectively remove DC from water. The objective of the study was to characterize the synergistic benefits of adsorption and biotransformation in removing DC from water using rice straw particles (RSPs) covered with DC degrading bacteria, Brevundimonas naejangsanensis strain DD1. First, optimal experimental conditions were identified for individual processes, i.e., hydrolysis, adsorption, and biotransformation, in terms of their performance of removing DC from water. Then, synergistic effects between adsorption and biotransformation were demonstrated by adding DD1-covered RSPs (DD1-RSPs) to DC-containing solution. Results suggest that DC was quickly adsorbed onto RSPs and the adsorbed DC was subsequently biotransformed by the DD1 cells on RSPs. The adsorption of DC to DD1-RSPs can be well described using the pseudo-second-order kinetics and the Langmuir isotherm. The DD1 cells on RSPs converted DC to several biotransformation products through a series of demethylation, dehydration, decarbonylation, and deamination. This study demonstrated that adsorption and biotransformation could work synergistically to remove DC from water.
研究领域:
环境科学
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微生物学
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CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Ying Han,Ning Pan,Deqiang Li,Shuhui Liu,Bin Sun,Jinling Chai,Dejie Li
What is the formation and stability mechanism of surfactant-free microemulsion (SFME)? What is the inherent relationship between three components to form SFME? These problems restrict the understanding, popularization and application of SFME. In this work, a new methyl salicylate/ethanol/water SFME is constructed as a representative for experimental and theoretical research. Results show that: the dynamic equilibrium of conservative, dissipative and random forces leads to the decrease of free energy of ternary system and promotes the formation of SFME. In oil in water (O/W) and water in oil (W/O) droplets, directional arrangement and charge redistribution of ethanol molecules in the interface layer can be induced by water. The induced charges on the O/W and W/O droplets are –48.999 and 2.697 a.u., which is conducive to the dispersion of droplets and improves the stability of SFME. When O/W droplets collide, an “electron trap” can be formed by water molecules, so that charge transfer can be carried out spontaneously. In addition, a novel parameter “ ƍ ” is defined representing the interaction between amphi -solvent, oil and water. The relationship of S (the proportion of single-phase area in ternary phase diagram) and ƍ is: S = 67.8 – 97.2 ƍ . ƍ less than 0.7 is the requirement for SFME to be formed and the maximum of S is 70%. The proposed regulation is verified by other 44 ternary systems. Hope this work would lay a theoretical foundation for explaining the stability mechanism, and provide guidance for developing more SFMEs.
研究领域:
化学
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材料科学
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ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2022
·
IF 11.357
Huiru Zhao,Meiling Wang,Yueting Cui,Chengdong Zhang
Silver nanoparticles (AgNPs) are effective antimicrobial substances that show promise in combatting multidrug resistance. The potential application and release of AgNPs into the environment may neutralize the selective advantage of antibiotic resistance. Systemic knowledge regarding the effect of NPs on the evolution of antibiotic resistance is lacking. Our results showed that bacteria slowly developed adaptive tolerance to ciprofloxacin (CIP) under cyclic CIP and silver ion (Ag+) cotreatment, and no resistance/tolerance was discernible when CIP and AgNP exposure was alternated. In contrast, rapid CIP resistance was induced under continuous selection by treatment with only CIP. To combat the effects of CIP and Ag+, bacteria developed convergent evolutionary strategies with similar adaptive mechanisms, including anaerobic respiration transitioning (to reduce oxidative stress) and stringent response (to survive harsh environments). Alternating AgNP exposure impeded evolutionary resistance by accelerating B12-dependent folate and methionine cycles, which reestablished DNA synthesis and partially offset high oxidative stress levels, in contrast with the effect of CIP-directed evolutionary pressure. Nevertheless, CIP/AgNP treatment was ineffective in attenuating virulence, and CIP/Ag+ exposure even induced the virulence-critical type III secretion system. Our results increase the basic understanding of the impacts of NPs on evolutionary biology and suggest prospective nanotechnology applications for arresting evolutionary antibiotic resistance.
研究领域:
微生物学
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纳米技术
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进化生物学
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抗菌研究
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MOLECULAR NUTRITION & FOOD RESEARCH
·
2021
·Vol. 66
·期 5
·2100615
·
IF 5.82
Xisha Lin,Hongliang Yao,Jingyu Guo,Yingying Huang,Wenjiao Wang,Binru Yin,Xiang'an Li,Ting Wang,Chunbao Li,Xinglian Xu,Guanghong Zhou,Josef Voglmeir,Li Liu
Scope Red meat intake is reported to be correlated with chronic diseases. A potential causal factor is N -glycolylneuraminic acid (Neu5Gc) which metabolically incorporates into diverse glycoconjugates in humans. This study aims to investigate the impact of exposure to Neu5Gc-rich red meat on healthy cytidine-5′-monophospho- N -acetylneuraminic acid hydroxylase ( Cmah ) knock-out mice and the underlying mechanisms. Methods and Results CMAH −/− mice are fed Neu5Gc-rich diet for short-term (4 months) and long-term (10 months). Health status and levels of inflammatory cytokines are assessed. Caco-2 cells are used to investigate the intestinal absorption of Neu5Gc-containing glycoprotein, and in vitro fermentation is used to investigate the Neu5Gc utilization by gut microbiota. Neu5Gc-rich diets show neither measurable abnormality in physio-biochemical and inflammatory indexes nor observable alterations of liver tissue in mice. Glycosylation of lactoferrin limits its intestinal epithelial absorption, and the absorption of Neu5Gc attach onto glycoprotein is thus limited. Neu5Gc is also simultaneously utilized by microorganisms under simulated gut conditions. Conclusion The results indicate that the long-term intake of Neu5Gc-rich red meat has no adverse effect on the health of CMAH −/− mice, which may be related to the limited absorption of Neu5Gc that is regulated by protein glycosylation, and the metabolism of Neu5Gc by gut microorganisms.
研究领域:
营养学
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免疫学
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微生物学
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分子生物学
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MOLECULAR PHARMACEUTICS
·
2023
·
IF 4.9
Shuting Li,Binbin Liu,Ziqiao Chen,Xiao Ou,Haowei Rong,Ming Lu
Identification of a thermodynamically stable polymorph is an important step in the early stage of drug development. Ritonavir (RIT) is a well-known case where the most stable polymorph II emerged after being marketed, leading to a loss of $250 million. Herein, we report the findings that routine melt crystallization can reveal the late-appearing polymorph II of RIT at small supercooling, but the probability of nucleation is very low. The addition of 30–50% polyethylene glycol (PEG) promotes the crystallization of Form II as the only phase at low supercooling, making it easier to detect in polymorphism screening. During the course of our research, a new polymorph, denoted Form III, was unexpectedly discovered, crystallizing as the major phase from neat RIT melts. Single crystals of Form III were grown from melt microdroplets. Benefiting from the ability of synchrotron radiation to detect weak diffraction signals that cannot be accessible by a laboratory diffractometer, a reasonable structure of Form III was solved with slight disorder relative to thiazole groups (P1 space group and Z′ = 4). The thermodynamic stability ranking of the three true polymorphs is Form II > Form I > Form III, as opposed to the order of solubility. The capacity to efficiently reveal rich polymorphs, especially the kinetically hindered polymorph, and rapidly grow single crystals of a new phase for structure determination together highlights the necessity of incorporating melt crystallization into routine methods for pharmaceutical polymorphism screening.
研究领域:
药学
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材料科学
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晶体学
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Huimei Pan,Boqiang Li,Jie Yang,Wenzhe Liu,Wang Luo,Baiyang Chen
This study revisited a list of inorganic iodine species on their detections and conversions under different water conditions. Several surprising results were found, e.g., UV–vis spectrophotometry is the only reliable method for I 3 – and I 2 determinations with coexisting I – /IO 3 – /IO 4 – , while alkaline eluent of IC and LC columns can convert them into I – completely; IO 4 – can be converted into IO 3 – completely in IC columns and partly in LC columns; a small portion of IO 3 – was reduced to I – in LC columns. To avoid errors, a method for detecting multiple coexisting iodine species is suggested as follows: firstly, detecting I 3 – and I 2 via UV–vis spectrophotometry; then, analyzing IO 4 – (> 0.2 mg/L) through LC; and lastly, obtaining I – and IO 3 – concentrations by deducting I − and IO 3 – measured by IC from the signals derived from I 3 – /I 2 /IO 4 – . As for stability, I − or IO 3 – alone is stable, but mixing them up generates I 2 or H 2 OI + under acidic conditions. Although IO 4 – is stable within pH 4.0–8.0, it becomes H 5 IO 6 /H 3 IO 6 2 – in strongly acidic/alkaline solutions. Increasing pH accelerates the conversions of I 3 – and I 2 into I – under basic conditions, whereas dissolved oxygen and dosage exert little effect. Additionally, spiking ICl into water produces I 2 and IO 3 – rather than HIO.
研究领域:
分析化学
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环境科学
·
水处理工程
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