Translational Oncology
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2025
·
IF 4.1
Zhe Qin,Fangzhou Ye,Jiayi Wang,Jun Jiang,Xiaohong Zhang,Huanqing Li,Li Feng
Background Cisplatin resistance significantly impedes the treatment of gastric cancer (GC). This work examined the possible therapeutic target status and function of BUB1B in controlling cisplatin resistance. Methods Following the identification of differentially expressed genes (DEGs), protein-protein interaction (PPI) network analysis was conducted using datasets from the Cancer Genome Atlas-stomach adenocarcinoma (TCGA-STAD), GSE51575, and GSE79973. Functional tests assessed the effect of BUB1B overexpression and knockdown on the GC cells. Enrichment analysis and RNA-seq identified pathways linked to BUB1B . Additionally, the function of BUB1B in GC cells resistant to cisplatin in regulating DNA repair was examined, as its relationship with Rad51 inhibitor (B02) in regulating cell cycle, proliferation, and apoptosis. The combined effects of Rad51 suppression and BUB1B overexpression on tumor development in cisplatin-resistant GC cells were further validated in vivo xenograft models. Results Significant overexpression of six critical overlapping genes was seen in GC tissues. The GC cell invasion, migration, and proliferation processes were improved by BUB1B overexpression, whereas BUB1B knockdown prevented these outcomes. Genes involved in DNA repair were downregulated by BUB1B knockdown, according to an RNA-seq study. BUB1B overexpression boosted cell survival via modulating cell cycle proteins, but BUB1B knockdown hampered DNA repair and increased death in cisplatin-resistant GC cells. Overexpression of BUB1B enhanced tumor development in vivo and counteracted the inhibitory effects of B02 on cell growth. Conclusion BUB1B enhances cisplatin resistance in gastric cancer by regulating DNA repair and cell cycle progression, suggesting that targeting BUB1B may be a feasible therapeutic strategy.
研究领域:
肿瘤学
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分子生物学
·
癌症治疗
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Siqi Zhang,Kun Wang,Kai-Bin Li,Wei Shi,Wen-Ping Jia,Xiaoying Chen,Ting Sun,De-Man Han
A silver nanoclusters (AgNCs)/graphene oxide (GO)-based fluorescence sensor was developed for label-free DNA detection through hybridization chain reaction (HCR). A DNA sequence associated with the human immunodeficiency virus (HIV) was selected as a model target. Two DNA probes , hairpin probe 1 (H 1 ) and hairpin probe 2 (H 2 ), were partially complementary. GO was used as an adsorption material to capture the hairpin probes and a selective fluorescence quencher was used to reduce the background signal. Upon addition of AgNO 3 and NaBH 4 , the AgNCs were synthesized at the terminals of the H 1 and H 2 probes. In the absence of target DNA (T HIV ), hybridization chain reaction (HCR) could not be triggered due to the stability of H 1 and H 2 probes. The hairpin probe-protected AgNCs attached to the GO surface, efficiently quenching fluorescence of the AgNCs. Therefore, the system showed very low background. In presence of T HIV , the target triggered the chain-like assembly of H 1 and H 2 through HCR, generating a long chain of H 1 and H 2 complexes. The HCR product (AgNCs nanowires) could not be adsorbed on the surface of GO; hence, it generated a strong fluorescent signal based on the concentration of the target. Under the optimized conditions, the detection limit of the fluorescence sensor was 1.18 nM, and hence it can be applied to clinical samples.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Qingfei Sun,Yueyue Gui,Chong Fan,Jiaxiong Li,Xiaomeng Tan,Chao Li,Jin Qiu,Jiehua Ma
Macrophages use pattern recognition receptors (PRRs) to recognize, capture, and phagocytize pathogens. Recreating artificial systems to mimic such receptors for manipulating macrophage predation is both scientifically exciting and technologically relevant to anti-infection. Nevertheless, fabricating synthetic PRR-mimicking receptors with a predictable and stable structure remains a challenge. Herein, we use circular aptamers as building blocks to create artificial DNA receptors (ADRs) that mimic the function of PRRs. After modification of ADRs on macrophages, they can stably recognize specific pathogens and promote the phagocytosis of macrophages, akin to natural PRRs. As dynamic structures, these ADRs can be flexibly activated or inactivated by external DNA molecules, akin to protein receptors responding to small-molecule ligands. Owing to the programmability of the DNA reaction, Boolean logic operations can be introduced to logically manipulate the predation behavior of macrophages, exhibiting the characteristics of artificial receptors. Furthermore, ADRs can be integrated with other functional DNA motifs, e.g., CpG DNA, to enhance the activation and antibacterial capacity of macrophages with higher efficiency. Overall, we believe that this artificial receptor not only broadens the application of DNA nanotechnology in cell biology but also contributes to ongoing efforts to remodel the innate immune system for fighting infection in consideration of the growing emergence of multidrug-resistant bacteria.
研究领域:
细胞生物学
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纳米技术
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免疫学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2541-2549
·
IF 4.58
Han Zhizhong,Luo Min,Chen Li,Pan Haibo,Chen Jinghua,Li Chunyan
The work describes a photoelectrochemical (PEC) biosensor for the detection of DNA. It employs oriented hierarchical ZnO flower-rod architectures (ZnO FRs) and DNA dendrimers. ZnO FRs act as photoactive material that yields a photocurrent of up to 23 μA. The photogenerated electron transfer is inhibited once the probe DNA and the target DNA oligonucleotides hybridize, and this results in a reduced photocurrent. The use of DNA dendrimers with scores of DNA branches further amplifies the signal of the PEC biosensor. The PEC sensor displays a response that is linear in the DNA concentration range from 10 fM to 0.1 μM with a detection limit of 3.7 fM (at S/ N = 3). The sensor was applied to the determination of DNA in human serum samples and was found to work with acceptable accuracy. Due to the use of ZnO FRs and DNA dendrimers, the assay is highly sensitive, rapid, and repeatability.
Schematic of an innovative photoelectrochemical (PEC) biosensors based on oriented ZnO flower-rod architectures (ZnO FRs) for DNA detection. Dendrimers were employed for signal amplification to give a low detection limit of 3.7 fM.
研究领域:
分析化学
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生物传感器
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材料科学
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纳米技术
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DOI
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 8
·2069-2083
·
IF 3.498
Nanfack Carole D. V.,Yang Jia,Yuan Xiao,Sun Jiadi,Sun Xiulan,Ji Jian
Deoxyribonucleic acid (DNA) extraction, which is the mainstay of molecular amplification for food pathogen detection procedure, is dependent on laboratory infrastructure, cumbersome methods, and time consumption. To tackle these dependencies, we developed an enhanced mussel-inspired cellulose card for the convenient, eco-friendly, and rapid DNA extraction from bacteria present in food. This enhanced cellulose mussel-inspired DNA adhesive card (BDA card) was successfully prepared using Na + through the self-polymerization of biopolymer polyvalent ions and 3,4-dihydroxy-l-phenylalanine (L-Dopa). We observed that, when the biopolymer surface modification of the cellulose was NaCl-doped, the DNA extraction and purification performances of the obtained BDA card were enhanced when compared to the performance of the cellulose modified with the biopolymer doped with HCl. After the surface modification, the previously modified cellulose card was characterized with the Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). The DNA extraction performances as well as the quality of the bacteria genomic DNA extracted were equally measured. The Na + -doped DNA adhesive cellulose card (BDA card) depicted the best bacteria genomic DNA extraction performances. When compared to commercially available bacteria genomic DNA extraction kits, the BDA card portrayed better DNA extraction performances, in a one-step extraction protocol. It allowed easy and convenient DNA extraction. The whole genomic DNA extraction process could be completed in 10 min. The concentration of the genomic DNA extracted from overnight bacteria culture was highest when extracted with the BDA card 459.39 ± 86 ng/µl as compared to Ezup and PureLink genomic DNA kits. When tested with spiked chicken, it was statistically equal to the Mag-MK food genomic DNA extraction kit. The proposed method provides a simple, efficient, eco-friendly, and convenient approach for bacteria genomic DNA extraction and purification from samples.
研究领域:
生物技术
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食品科学
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分子生物学
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DOI
高影响因子
TALANTA
·
2016
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IF 4.035
Yuhong Liu,Linlin Zhao,Jin Zhang,Jinzha Zhang,Wenbo Zhao,Chun Mao
The work investigates a new fluorescence resonance energy transfer (FRET) system using NaEuF 4 nanoparticles (NPs) and [email protected] 2 S NPs as the energy donor–acceptor pair for the first time. The NaEuF 4 / [email protected] 2 S NPs-based FRET DNA sensor was constructed with NaEuF 4 NPs as the fluorescence (FL) donor and [email protected] 2 S core−shell NPs as FL acceptor. In order to find the matching energy acceptor, the amount of AgNO 3 and Na 2 S were controlled in the synthesis process to overlap the absorption spectrum of energy acceptor with the emission spectrum of energy donors. The sensitivity of FRET-based DNA sensor can be enhanced and the self-absorption of ligand as well as the background of signals can be decreased because of Eu 3+ which owns large Stokes shifts and narrow emission bands due to f–f electronic transitions of 4f shell. We obtained the efficient FRET system by studying suitable distance between the donor and acceptor. Then the FRET-based DNA sensor was used for the design of specific and sensitive detection of target DNA and the quenching efficiency (ΔFL/F 0 , ΔFL=F−F 0 ) of FL was logarithmically related to the concentration of the target DNA, ranging from 100 aM to 100 pM. We can realize an ultrasensitive detection of target DNA with a detection limit of 32 aM. This proposed method was feasible to analyse target DNA in real samples with satisfactory results.
研究领域:
生物化学
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分子生物学
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纳米技术
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DOI
PubMed
高影响因子
JACS Au
·
2025
·
IF 8.7
Weiming Lin,Tao Ding,Die He,Nan Zhang,Haodong Li,Wenjian Luo,Zhongxia Wei,Min Ke,Sisi Jia,Chunhai Fan,Le Liang
Nanodiamonds (NDs) with nitrogen-vacancy (NV) centers are emerging as powerful quantum nanosensors (QNs) in biomedical applications due to their exceptional sensitivity. However, achieving optimal diagnostics performance necessitates both high sensitivity and selectivity; especially in practical biomedical settings, it remains challenging for QNs to provide quantitative analyses when multiple analytes are present. Here, we present a biosensing platform that integrates DNA logic gates (DLGs) with spin-based quantum sensing, termed DLG-QN for ultrasensitive and ultraselective diagnostics. Utilizing an AND DLG, both NDs and magnetic beads (MBs) are functionalized with hairpin DNA strands. In the presence of both miRNA-21 and miRNA-155─key biomarkers overexpressed in cancer─the hairpin DNAs undergo conformational changes that facilitate DNA-guided self-assembly of NDs and MBs, enriching the target signal. Resonant microwave modulation of ND fluorescence emission allows for high signal-to-noise ratio (SNR) detection by separating the signal from background fluorescence via spin-enhanced analysis. This platform demonstrated ultrasensitive and ultraselective detection of miRNA-21 and miRNA-155 with a limit of detection of 19.8 fM, highlighting its potential as a general biosensing strategy for precision diagnostics involving multiple biomarkers.
研究领域:
生物医学工程
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分子诊断学
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量子生物学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 6
·1-8
·
IF 5.833
Wang Hai-Bo,Mao An-Li,Tao Bei-Bei,Zhang Hong-Ding,Xiao Zhong-Liang,Liu Yan-Ming
An interesting phenomenon is described that the fluorescence signal of poly(adenine) (A) DNA-templated gold nanoclusters (AuNCs) is greatly improved in the presence of l -histidine by means of l -histidine-DNA interaction. The modified nanoclusters display strong fluorescence emission with excitation/emission maxima at 290/475 nm. The fluorescence quantum yield (QY) is improved from 1.9 to 6.5%. Fluorescence enhancement is mainly ascribed to the l -histidine-DNA interaction leading to conformational changes of the poly(A) DNA template, which offer a better microenvironment to protect AuNCs. The assay enables l -histidine to be determined with good sensitivity and a linear response that covers the 1 to 50 nM l -histidine concentration range with a 0.3 nM limit of detection. The proposed method has been applied to the determination of imidazole-containing drugs in pharmaceutical samples. Graphical abstract
A turn-on fluorescent method has been designed for the sensitive detection of l -histidine as well as imidazole-containing drugs on the basis of the l -histidine-DNA interaction.
研究领域:
纳米技术
·
分析化学
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生物化学
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Tongtong Guo,Yuanhang Xiang,Hao Lu,Minmin Huang,Fengfei Liu,Min Fang,Jia Liu,Yujin Tang,Xinchun Li,Fan Yang
Interfacial DNA self-assembly is fundamental to solid nucleic acid biosensors, whereas how to improve the signal-to-noise ratio has always been a challenge, especially in the charge-based electrochemical DNA sensors because of the large noise from the negatively charged DNA capture probes. Here, we report a DNA framework-reversed signal-gain strategy through background-to-signal transition for ultrasensitive and highly specific electrical detection of microRNAs (miRNAs) in blood. By using a model of enzyme-catalyzed deposition of conductive molecules (polyaniline) targeting to DNA, we observed the highest signal contribution per unit area by the highly charged three-dimensional (3D) tetrahedral DNA framework probe, relative to the modest of two-dimensional (2D) polyA probe and the lowest of one-dimensional (1D) single-stranded (ss)DNA probe, suggesting the positive correlation of background DNA charge with signal enhancement. Using such an effective signal-transition design, the DNA framework-based electrochemical sensor achieves ultrasensitive miRNAs detection with sensitivity up to 0.29 fM (at least 10-fold higher than that with 1D ssDNA or 2D polyA probes) and high specificity with single-base resolution. More importantly, this high-performance sensor allows for a generalized sandwich detection of tumor-associated miRNAs in the complex matrices (multiple cell lysates and blood serum) and further distinguishes the tumor patients (e.g., breast, lung, and liver cancer) from the normal individuals. These advantages signify the promise of this miRNA sensor as a versatile tool in precision diagnosis.
研究领域:
生物化学
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分子生物学
·
分析化学
·
生物医学工程
·
癌症诊断
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产品关联
DOI
PubMed
高影响因子
BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-19
·
IF 4.8
Zhang Xiaoxiao,Zhu Lili,Xu Zihan,Wang Zhimin,Dai Liping
Background DNA methylation plays a key role in regulating plant growth and development, secondary metabolism, and environmental adaptability and is affected by DNA methyltransferase (C5-MTase) and demethylase (dMTase). However, the response of C5-MTase and dMTase genes to abiotic stress in Isodon rubescens remains largely unknown. Results In this study, eight IrC5-MTase genes and five IrdMTase genes were identified within the genome of I. rubescens . The IrC5-MTases were phylogenetically divided into four subfamilies (CMT, DRM, MET and DNMT2), and the IrdMTase were divided into two subgroups (ROS and DML) on the basis sequence similarity, conserved motif and domain architecture. The IrC5-MTase and IrdMTase genes were unevenly distributed on 5 and 4 chromosomes, respectively. Cis‑acting element analysis revealed that the IrC5-MTase and IrdMTase genes were enriched with multiple light-, phytohormone- and stress-responsive elements. Importantly, the level of DNA methylation in I. rubescens was influenced by different 5-azacytidine (5-azaC) concentrations or light intensities but unaffected by different NaCl concentrations. Accordingly, the expression of IrC5-MTase and IrdMTase genes were significantly upregulated or downregulated in response to different concentrations of 5-azaC or amounts of light stress, however, no conspicuous differences were observed under different degrees of NaCl stress. Expression correlation analysis revealed that the expression of IrCMT2a , IrMET1 , and IrDML4 was most closely related to the DNA methylation level in I. rubescens . Conclusions Our results reveal the characteristics and differential expression of the IrC5-MTase and IrdMTase genes in response to 5-azaC, light or NaCl stresses and will facilitate future epigenetic studies in I. rubescens .
研究领域:
植物学
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遗传学
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表观遗传学
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DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2025
·Vol. 172
·期 4
·045502
·
IF 3.3
Lingling Zhang,Shuangru Yang,Yuge Liu,Yongmei Jia,Zhiguo Li
Understanding the electron transfer performance of DNA self-assembled monolayers (SAMs) on a transducer substrate was important for constructing DNA electrochemical sensors with high sensitivity. Here we immersed the gold electrodes in mixed solutions comprising thiol-modified stem-loop DNA (SL-DNA) or linear DNA (L-DNA) terminally bound with methylene blue (MB), and aliphatic thiols (HOC6SH, C6SH, HOOCC5SH, H2NC6SH, HOC11SH, C12SH, or HOOCC10SH) to obtain DNA/thiols mixed self-assembled monolayers (SAMs). MB electron transfer performances through SL-DNA/thiols and L-DNA/thiols mixed SAMs were investigated by cyclic voltammetry (CV). Our results showed that the mixed assembly of thiols with DNA was conducive to MB electron transfer. The electron transfer rate (ks) values of MB through L-DNA SAMs were bigger than those through SL-DNA SAMs, indicating that the flexibility of DNA chains was important to MB electron transfer. Thiols chain length or terminal groups influenced ks of MB significantly, that increasing NaCl or MgCl2 concentrations in solution around DNA availed MB electron transfer, and Mg2+ advanced MB electron transfer more than Na+, and that the stability of L-DNA/thiols SAMs was much better than SL-DNA/thiols SAMs. These conclusions might provide a reference for studying DNA electron transfer mechanisms or designing DNA or RNA electrochemical sensors with optimal performance.
研究领域:
生物化学
·
电化学传感器
·
分子生物学
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DOI
高影响因子
Scientific Reports
·
2018
·Vol. 8
·期 1
·1-8
·
IF 4.122
Zhang Hong,Li Xintong,He Fan,Zhao Mingqin,Ling Liansheng
A novel turn-off colorimetric sensor for sequence-specific recognition of single-stranded DNA (ssDNA) was established by combining Y-shaped DNA duplex and G-quadruplex-hemin DNAzyme. A G-rich single-stranded DNA (Oligo-1) displays peroxidase mimicking catalytic activity due to the specific binding with hemin in the presence of K + , which was able to catalyze the oxidation of colorless 2,2′-azinobis(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS 2− ) by H 2 O 2 to generate green ABTS− radical for colorimetric assay. Oligonucleotide 2 (Oligo-2) was partly complementary with Oligo-1 and the target DNA. Upon addition of target DNA, Oligo-1, Oligo-2 and target DNA can hybridize with each other to form Y-shaped DNA duplex. The DNAzyme sequence of Oligo-1 was partly caged into Y-shaped DNA duplex, resulting in the inactivation of the DNAzyme and a sharp decrease of the absorbance of the oxidation product of ABTS 2− . Under the optimum condition, the absorbance decreased linearly with the concentration of target DNA over the range of 1.0–250 nM and the detection limit was 0.95 nM (3σ/slope) Moreover, satisfied result was obtained for the discrimination of single-base or two-base mismatched DNA.
研究领域:
生物化学
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分子生物学
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纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
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