ACS Chemical Neuroscience
·
2021
·
IF 4.418
Qi Li,Jun Mo,Baichen Xiong,Qinghong Liao,Ying Chen,Yuanyuan Wang,Shuaishuai Xing,Siyu He,Weiping Lyu,Ning Zhang,Haopeng Sun
Tyrosinase is involved in the synthesis of neuromelanin in the substantia nigra, which is closely correlated with the pathogenesis of Parkinson’s disease. Herein, we identified S05014 (l-Tyr, IC50 = 6.25 ± 1.43 nM; l-Dopa, IC50 = 0.64 ± 0.40 μM) as a highly effective tyrosinase inhibitor. It could inhibit the tyrosinase function from different origins and decrease the expression of tyrosinase. S05014 presented good medication safety and inhibited melanogenesis in a dose-dependent manner. Moreover, as a resorcinol derivative, S05014 could scavenge the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and significantly reduce the overproduction of LPS-induced reactive oxidative species (ROS), indicating its antioxidative profile. S05014 exhibited an excellent neuroprotective effect against methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) impairment in vitro and could remarkably alleviate movement abnormalities and exploratory activities in vivo. Altogether, S05014 is considered as a promising inhibitor for tyrosinase, melanogenesis, and oxidative stress and has great potential to be utilized in anti-Parkinsonian syndrome. From this point of view, tyrosinase inhibition has been further confirmed to be a novel strategy to improve locomotor capacity and treat Parkinson’s disease.
研究领域:
神经科学
·
药理学
·
药物发现
·
生物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Shang Gao,Tiantian Li,Zhao-Rong Li,Bingwu Liao,Mouming Zhao,Chunxia Zhou,Donghui Luo,Rui-Bo Jia
Coreopsis tinctoria buds grow at high altitudes and have unique medicinal and health effects. This study focused on the chemical components of gradient ethanol extracts of Coreopsis tinctoria buds and their impact on the gut microbiota and its metabolites. UPLC-Q-Exactive-MS results disclosed the presence of 24 distinct chemicals in the extracts, marein, and quercetin-3- β -D-glucoside as the predominant antioxidants. Furthermore, the anhydrous ethanol extract (EtOH-EX) and 80 % ( w /w) ethanol extract (80 % EtOH-EX) showed the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) free radical scavenging capacity, and the water extract (Water-Ex) showed excellent bile acid binding capacity. Besides, simulated fermentation of the extracts increased short-chain fatty acids and the abundance of gut-friendly bacteria like Bifidobacteria , especially in the Water-EX group. Correlation analysis revealed that the key microbiota on metabolites production. In conclusion, the extracts derived from Coreopsis tinctoria buds exhibit a spectrum of bioactivities, viewing them as flexible options for applications across food and pharmaceutical products.
研究领域:
药理学
·
食品科学
·
微生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Xiaoming Song,Dan Feng,Jiamin Li,Liyan Zang,Hongmei Li,Jing Sun
This study integrates HPLC fingerprinting and NIR spectroscopy to evaluate the antioxidant capacity of Rheum tanguticum . HPLC fingerprinting comprehensively characterized the complex chemical composition, identifying 13 compounds. Meanwhile, NIR spectroscopy provided a rapid, non-destructive approach to predict antioxidant capacity. The combination of these techniques offers a holistic and efficient strategy for evaluating antioxidant activity. The spectrum-effect relationships were analyzed by correlating the results of three antioxidant assays (ABTS, DPPH, and FRAP) with the peak areas of the common peaks identified by HPLC. Glycosylated anthraquinones, particularly conjugated forms like rhein-8-O-glucoside, were found to synergistically enhance antioxidant activity with free aglycones. NIR models optimized using the PLS method with Python-based algorithms demonstrated strong predictive abilities, achieving RPD values of 2.43 for ABTS, 2.63 for DPPH, and 2.43 for FRAP. This research provides a rapid method to evaluate the antioxidant capacity of Rh. tanguticum , offering a reference for future applications in other fields.
研究领域:
药物分析
·
光谱学
·
色谱学
·
药物化学
·
计算生物学
·
植物科学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2012
·Vol. 17
·期 12
·14748-14764
·
IF 2.386
Rui Liu,Bin Zhao,Dong-En Wang,Tianyu Yao,Long Pang,Qin Tu,Jian-Jun Liu,Jinyi Wang
A series of nitrogen-containing apigenin analogs4a–jwas synthesized via Mannich reactions to develop anticancer, antibacterial, and antioxidant agents from plant-derived flavonoids. The chemical structures of these compounds were confirmed using1H-NMR,13C-NMR, and ESI-MS. Thein vitrobiological activities of the analogs were evaluated via assays of their antiproliferative, antibacterial, and antioxidant activities. The prepared apigenin analogs exhibited different antiproliferative activities against four human cancer cell lines, namely human cervical (HeLa), human hepatocellular liver (HepG2), human lung (A549), and human breast (MCF-7) cancer cells. Compound4ishowed the most favorablein vitroantiproliferative activity with IC50values of 40, 40, 223, and 166 μg/mL against HeLa, HepG2, A549, and MCF-7, respectively. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay also showed that4ihad the most potent antioxidant activity, with the smallest IC50value (334.8 μg/mL). The antibacterial activities of the analogs were determined using a two-fold serial dilution technique against four pathogenic bacteria, namelyStaphylococcus aureus,Bacillus subtilis,Escherichia coli, andPseudomonas aeruginosa. All the prepared apigenin analogs exhibited more potent activities than the parent apigenin. Compounds4hand4j, in particular, exhibited the best inhibitory activities against the Gram-positive bacteriaStaphylococcus aureusandBacillus subtiliswith MIC values of 3.91 and 1.95 μg/mL, respectively.Keywords:apigenin analogs;antiproliferative activity;antibacterial activity;antioxidant activity
研究领域:
药理学
·
化学
·
肿瘤学
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微生物学
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产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2018
·
IF 2.616
You Luo,Bin Peng,Yan Liu,Yanan Wu,Zhenqiang Wu
Plant polysaccharides possess multiple physiological activities. Box-Behnken design (BBD) was employed to optimize the ultrasound-assisted extraction of polysaccharides from guava leaves (GLP). It was found that ultrasonic time, temperature and power has a significant effect on the extraction efficiency. The yield of GLP was 1.00 ± 0.04% under the optimal condition of extraction time of 20 min, temperature of 62 ℃ and ultrasound power of 404 W. The DPPH and ABTS + radical scavenging rate of GLP was 56.38% and 51.73% at 100 μg/mL, respectively. The rate of inhibition of α-amylase by GLP was only 14.06% at 1 mg/mL; however, its α-glucosidase inhibition rate reached 99.54% at 100 μg/mL. These results suggested that the ultrasound-assisted extraction for GLP is effective and environmentally friendly and GLP has wide application potentials as natural antioxidants and α-glucosidase inhibitors.
研究领域:
药理学
·
食品科学
·
植物科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2019
·Vol. 43
·期 12
·e14277
·
IF 1.288
Xiao Gong,Xiaobing Huang,Taohua Yang,Jing Wen,Wei Zhou,Jihua Li
The effects of hot air drying (HD), freeze-drying (FD), microwave drying (MWD), natural convective drying (NCD), and vacuum drying (VD) on the physicochemical, nutritional, and antioxidant properties of okra pods were investigated. Changes in the surface morphology, color, rehydration ratio, protein, pectin, ascorbic acid, and phenolics, as well as antioxidant activities of okra pods measured by 2,2′-azinobis (3-ethylbenzo thiazoline-6-sulphonic acid) diammonium salt (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and Ferric Ion Reducing Antioxidant Power (FRAP) assays were evaluated. In general, FD products exhibited a better retention of the texture characteristics, nutritional, and antioxidant activities, and was suitable for okra pods drying, although FD was the longer time-consuming method among the five drying methods. Furthermore, NCD products were poorer in color, texture, and nutrition. Future studies should continuously focus on various combinations of different types of drying methods to supply okra pods products with the most affordable cost and the highest quality. Practical applications Physicochemical properties and antioxidant capacities of okra pods are affected by the drying process. The drying process with nonthermal, low temperature, and low content of oxygen is beneficial to the retention of nutrients and functional components. In general, freeze-drying exhibited a better texture characteristics and good antioxidant activities, although higher energy and longer time consumption was the current challenge. How to combine the different types of drying methods has become an inevitable trend and research focus.
研究领域:
食品科学
·
农业工程
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
Food Science and Human Wellness
·
2023
·
IF 7
Jie Ding,Chunyan Zhu,Pengfei Jiang,Libo Qi,Na Sun,Songyi Lin
Enzymatic hydrolysis, isolation, and purification might make a great deal of difference in antioxidant activity and antigenicity of peptide components. This study aimed to isolate and purify antioxidant peptide components from Antarctic krill and evaluate their allergenicity of them. Electron paramagnetic resonance (EPR) spectroscopy results indicated 3 10 kDa Antarctic krill hydrolysates (AKHs) had higher DPPH and · OH radical scavenging rates. And the second component (N2-2) purified 3 10 kDa hydrolysate showed better ability to scavenge DPPH and · OH radicals ( P < 0.05), which were (47.43 ± 2.18)% and (34.33 ± 1.25)%, respectively. Additionally, indirect-ELISA results revealed that N2-1 had a weaker ability to bind specific IgE and that N2-2 had a lower binding capability to specific IgG1 ( P < 0.05). And N2-2 had a higher EC 50 value of (5.29 ± 0.95) ng/mL ( P < 0.05) in cell degranulation assay, which was about 13.80 times that of Antarctic krill. Therefore, N2-2 might be the potential source of the antioxidant peptides with lower allergenicity.
研究领域:
食品科学
·
生物化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zhongwen Su,Yonggang Peng,Ying Yu,Yujing Zhong,Junyue Zeng,Yikai Chen,Sekar Vijayakumar,Yangfan Mao,Lin Wang,Meihua Xin,Mingchun Li
In this study, three chitosan quaternary phosphonium salts and three chitosan quaternary ammonium salts with varying spacer chain lengths were synthesized. Structure characterization of the obtained products was conducted using Fourier-transform infrared spectroscopy, nuclear magnetic resonance, thermogravimetric analysis, and elemental analysis. Additionally, the in vitro scavenging efficiency of these derivatives against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radicals, as well as their antibacterial activities against Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ), were evaluated. The results indicated that the scavenging efficiency of the chitosan quaternary phosphonium salt at a concentration of 1.6 mg/mL ranged from 36.9 % to 48.7 % for DPPH free radicals and from 48.3 % to 66.3 % for superoxide free radicals, outperforming that of the chitosan quaternary ammonium salt with long alkyl chains. Furthermore, an increase in the length of the spacer alkyl chain was associated with enhanced antibacterial activities of the corresponding chitosan derivatives. At a concentration of 1.0 mg/mL, the synthesized chitosan derivatives demonstrated antibacterial rates exceeding 90 % against both E. coli and S. aureus . Notably, the CCK-8 assay confirmed that these derivatives are non-toxic to 293 T cells. Among the six derivatives, TPPBOC and DMDOC, characterized by longer spacer alkyl chains, demonstrated superior antioxidant and antibacterial activities. This research establishes a robust theoretical foundation for the development of more effective and safer antioxidants and antibacterial agents, thereby enriching the knowledge base in chitosan chemistry and functional materials, and paving new pathways for the innovation of novel antioxidant and antibacterial materials.
研究领域:
生物化学
·
材料科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 8
·1158
·
IF 4.7
Yuzhu Zhao,Doudou Liu,Jiaxuan Zhang,Jiaxin Shen,Jiamin Cao,Huawei Gu,Mengqing Cui,Ling He,Gong Chen,Shuwen Liu,Kan Shi
Grape pomace seeds contain abundant phenolic compounds, which are also present in both soluble and insoluble forms, similar to many other plant matrices. To further increase the extractable soluble phenolics and their antioxidant activities, grape pomace seeds were fermented with different fungi. Results showed that solid-state fermentation (SSF) withAspergillus niger,Monascus anka, andEurotium cristatumat 28 °C and 65% humidity had a significantly positive impact on the release of soluble phenolics in grape pomace seeds. Specifically, SSF withM. ankaincreased the soluble phenolic contents by 6.42 times (calculated as total phenolic content) and 6.68 times (calculated as total flavonoid content), leading to an overall improvement of antioxidant activities, including DPPH (increased by 2.14 times) and ABTS (increased by 3.64 times) radical scavenging activity. Furthermore, substantial changes were observed in the composition and content of individual phenolic compounds in the soluble fraction, with significantly heightened levels of specific phenolics such as chlorogenic acid, syringic acid, ferulic acid, epicatechin gallate, and resveratrol. Notably, duringM. ankaSSF, positive correlations were identified between the soluble phenolic content and hydrolase activities. In particular, there is a strong positive correlation between glycosidase and soluble phenols (r = 0.900). The findings present an effective strategy for improving the soluble phenolic profiles and bioactivities of grape pomace seeds through fungal SSF, thereby facilitating the valorization of winemaking by-products.
研究领域:
食品科学
·
生物技术
·
化学
·
农业
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Express
·
2024
·Vol. 14
·期 4
·583-589
·
IF 0.7
Luo Yao,Wu Wei-Gang,Tian Feng,Huang Jun
Excessive oxidative stress leading to a consequently chronic inflammation remains a challenging problem for wound healing, and developing biomaterials with outstanding antioxidant property has great practical significance for wound treatment. Herein, a thermo-sensitive hydrogel based
on Schiff base linkages (Cur@HBC/OHA) was prepared, and the physicochemical properties and antioxidant activities in vitro were investigated. The excellent biocompatibility, enhanced antibacterial activity and increased DPPH scavenging rate indicated that the prepared Cur@HBC/OHA hydrogel
is a promising biomaterial for tissue engineering applications.
研究领域:
生物材料科学
·
组织工程
·
药学
✓ 已验证
DOI
高影响因子
JOURNAL OF BIOLOGICAL CHEMISTRY
·
2024
·
IF 4
Qian Hu,Yanan Zhao,Wan-yang Sun,Zexian Ou,Wentao Duan,Zeyu Qiu,Yuanlong Ge,Daolin Tang,Tianfeng Chen,Xiang Cheng,Rong-rong He,Shu Wu,Zhenyu Ju
Ferroptosis is a type of regulated cell death driven by iron-dependent accumulation of lipid peroxidation, exhibiting unique morphological changes. While actin microfilaments are crucial for various cellular processes, including morphogenesis, motility, endocytosis, and cell death, their role in ferroptosis remains unclear. Here, our study reveals that actin microfilaments undergo remodeling and disassembly during ferroptosis. Interestingly, inhibitors that target actin microfilament remodeling do not affect cell sensitivity to ferroptosis, with the exception of CK-666 and its structural analogue CK-636. Mechanistically, CK-666 attenuates ferroptosis independently of its canonical function in inhibiting the Arp2/3 complex. Further investigation revealed that CK-666 modulates the ferroptotic transcriptome, prevents lipid degradation, and diminishes lipid peroxidation. In addition, CK-666 does not impact the labile iron pool within cells, nor does the inhibition of FSP1 impact its anti-ferroptosis activity. Notably, the results of DPPH assay and liposome leakage assay suggest that CK-666 mitigates ferroptosis by directly eliminating lipid peroxidation. Importantly, CK-666 significantly ameliorated renal ischemia-reperfusion injury and ferroptosis in renal tissue, underscoring its potential therapeutic impact.
研究领域:
细胞生物学
·
药理学
·
肾脏生理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Aquatic Food Product Technology
·
2017
·
IF 0.478
Jia-Run Han,Zhi-Mo Zhu,Hai-Tao Wu,Na Sun,Yue Tang,Cui-Ping Yu,Chen-Chen Zhao,Zheng-Yu Zhang,Ao-Ting Li,Jia-Nan Yan
Maillard reaction products (MRPs) were produced in sea cucumber gut hydrolysates (SCGHs)-ribose system at 75°C, 85°C, and 95°C with a pH value of 7.0 or 8.0 for 12 hour. The parameters of amount of intermediate products, fluorescence intensity, browning development, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability showed a positive correlation with each other in SCGHs-ribose system. The amount of intermediate products, fluorescence intensity, and browning development followed the zero-order kinetic with activation energy from 62 to 91 kJ/mol in the Maillard reaction of SCGHs-ribose system. Moreover, 2-methyl-butanal has positive and significant correlation with DPPH radical scavenging ability of SCGHs-ribose MRPs. These results suggest that Maillard reaction improves the antioxidant activity of SCGHs, which contributes to the generation of intermediate, fluorescent, browning, and volatile products of SCGHs-ribose system.
研究领域:
食品化学
·
营养科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）