JOURNAL OF HAZARDOUS MATERIALS
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2021
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IF 10.588
Xueping Huang,Aiping Wang,Jian Luo,Yue Gao,Lei Guan,Peng Zhang,Feng Liu,Wei Mu,Beixing Li
Recently, size-controlled pesticide microcapsule (MC) delivery systems have played an important role in precision farming development; however, the potential environmental hazards of MCs with different particle sizes have not been fully characterized. In this study, we prepared a series of lambda-cyhalothrin (LC)-MCs with nano and micron-scale capsule sizes (average diameters of 209.4 nm, MC-N; 2.41 µm, MC-S; 4.87 µm, MC-M; and 12.41 µm, MC-L). The assessment results showed that the release and sedimentation behavior of LC-MCs in water and toxicity to zebrafish at three life stages were all particle size-dependent. As the diameter distribution of approximately 100 nm extended to the micron scale (~27 µm), the capsules released more slowly and sunk more quickly in water. In addition, micron-sized LC-MC exposure resulted in significantly less fish mortality and malformations of larvae and embryos compared with nanosized LC-MC exposure. The highest accumulation of MC-N in the gill and the severest toxicity to larvae suggested that the smaller size and stronger permeability of nanocapsules would pose unpredictable consequences for nontargeted organisms. The obvious toxicological differences of LC-MCs toward aquatic organisms implies that regulating MC production in an appropriate size range is an important prerequisite for achieving efficient but safe pesticide applications .
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PubMed
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
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IF 6.1
Wei Liu,Yanlei Yang,Jingyu Han,Yiyi Feng,Junguo Ma,Xiaoyu Li
The coexistence of glyphosate (GLY) and microcystin-LR (MC-LR) in the environment has become a problem that cannot be ignored. However, the combined toxic effects of these compounds on aquatic organisms remain underexplored. The purpose of this study was to evaluate the individual and combined effects of GLY and MC-LR on the neurobehavior of exposed adult zebrafish and their untreated offspring (F1 larvae), as well as to explore the underlying mechanisms. In this study, six month old male zebrafish were exposed to 3.5 mg/L GLY and 35 μg/L MC-LR, alone and in combination, for 21 d. The results revealed that exposure to GLY and/or MC-LR decreased locomotor activity, heightened anxiety-like behaviors, and disrupted social interactions in adult zebrafish, thereby confirming their neurotoxic effects. Pathological damage, along with elevated levels of ROS, IL-1β, and caspase-3, were observed in the brains of the treated fish. RNA-seq analysis further revealed that GLY and/or MC-LR treatment induced widespread transcriptional changes in the zebrafish brain, potentially impacting a range of biological functions, including neurobehavior. Meanwhile, paternal exposure to GLY and MC-LR, either individually or in combination, inhibited the spontaneous movement of untreated embryos at 24 hpf and reduced swimming activity at 120 hpf. Transcriptome sequencing of larvae further elucidates the intricate mechanisms underlying the intergenerational neurotoxicity induced by GLY and/or MC-LR, and the metabolic alterations potentially play an important role in mediating this toxicity. Overall, these findings collectively provide robust evidence of intergenerational effects resulting from exposure to individual and combined GLY and MC-LR, especially regarding persistent impacts on behavioral parameters, which should be emphasized in future ecological and health risk assessments.
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ENVIRONMENTAL POLLUTION
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2025
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IF 7.3
Yufei Xie,Qiuyu Liu,Wenting Lin,Yuan Ren,Lingtian Xie
Various anabolic androgenic steroids (AAS) are frequently detected in aquatic environments, yet the potential ecotoxicological impacts of their increased load have not received adequate attention. This study investigates the effects of exposure to the androgen nandrolone on sex differentiation, gonadal development, and the expression of hormones, enzymes, and proteins related to the hypothalamic-pituitary-gonadal (HPG) axis in zebrafish. The results indicate that exposure to nandrolone at 60 days post-fertilization promotes the maturation of spermatogonia, inhibits the development of oocytes, and induces their apoptosis. Additionally, there were dose-dependent changes in male differentiation and hormone levels within the HPG axis: in the high-dose group (5 μg/L), males constituted 73 % of the population, and levels of E2 hormones in gonadal tissues decreased by 33 %, while T hormone levels increased by 55 %. Furthermore, the expression of genes essential for ovarian differentiation were downregulated, whereas the expression of testicular differentiation-related genes were upregulated. The molecular docking results show that the drug can form hydrogen bonds with key proteins, which have high affinity, and are more likely to cause conformational changes in the proteins, thereby altering their activity. KEGG analysis suggests that the MAPK signaling pathway is likely the target pathway through which nandrolone exposure induces reproductive toxicity.
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PubMed
高影响因子
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