SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Mei Zhang,Qianqian Kong,Jingcheng Huang,Yaodong Xiang,Guanjie Wang,Jie Han,Yemin Guo,Shancang Zhao,Xia Sun
Within this work, an electrochemiluminescence (ECL) aptasensor was constructed for the sensitive detection of diazinon . Primarily, AuNPs not only provided a platform for luminol fixation due to their favorable bioactivity and conductivity , but they facilitated the improvement of ECL signals. Furthermore, three-dimensional (3D) flower-like ZnONPs were obtained by hydrothermal synthesis , which could be an efficacious catalyst for the luminol-hydrogen peroxide (luminol-H 2 O 2 ) system, whereby the ECL signal of the sensor could be amplified. Given the large specific surface area of 3D flower-like ZnONPs, modification of positive charges on their surface could well immobilized the electronegative aptamer chains on the electrode surface , and when diazinon (DZN) was added, the aptamer combined with DZN to form a complex to hinder the ECL intensity, and the detection of DZN was achieved by the change of ECL signal intensity. After evaluated the experimental parameters, the detection limit of DZN was 0.0109 nM (S/N = 3), and the linear range was 0.0328–3.28 × 10 4 nM. The proposed ECL aptasensor had comparatively satisfactory detection limits, which provided a reasonable reference for other contaminant residues detection.
研究领域:
分析化学
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环境科学
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食品安全
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纳米技术
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生物化学
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DOI
高影响因子
LUMINESCENCE
·
2022
·Vol. 37
·期 6
·980-986
·
IF 2.613
Weiwei Luo,Zhuoxin Ye,Daqian Song,Pinyi Ma
In this work, an electrochemiluminescence (ECL) sensor chip for sensitive detection of thrombin (TB) was prepared using a screen-printed electrode (SPE) as a working electrode and an aptamer as a specific recognition moiety. To produce an ECL sensor chip, a layer of p L -Cys was immobilized on the surface of the SPE using the cyclic voltammetry scanning method. A layer of gold nanoparticles (AuNPs) was assembled through an Au–S bond and hairpin DNA was further immobilized on the electrode surface. Ru(bpy) 2 (mcpbpy) 2+ , as a luminescent reagent, was covalently bound to single-stranded DNA (ssDNA) to prepare a luminescence probe ssDNA-Ru. The probe was hybridized with TB aptamer to form a capture probe. In the presence of TB, the TB aptamer in the capture probe bound to TB, causing the release of ssDNA-Ru that could bind to hairpin DNA on the electrode surface. The Ru(II) complex as a luminescent reagent was assembled onto the electrode, and p L -Cys was used as a co-reactant to enhance the ECL efficiency. The ECL signal of the sensor chip generated based on the above principles had a linear relationship with log TB concentration at the range 10 fM to1 nM, and the detection limit was 0.2 fM. Finally, TB detection using this method was verified using real blood samples. This work provides a new method using an aptamer as a foundation and SPE as a material for the detection of biological substances.
研究领域:
分析化学
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生物传感器
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纳米技术
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生物化学
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PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Yu Liu,Qiu-Yue Cheng,Hang Gao,Hong-Yuan Chen,Jing-Juan Xu
A microfluidic gradient array is a widely used screening and analysis device, which has characteristics of high efficiency, high automation, and low consumption. Bipolar electrode electrochemiluminescence (BPE-ECL) has special value in microfluidic array chips. The combination of the microfluidic gradient and BPE arrays has potential for high-throughput screening. In this article, a microfluidic BPE array chip for gradient culture and conditional screening of cancer cells was designed. The generation of concentration gradients, continuous culture of cancer cells with high throughput, and drug screening through BPE-ECL of the Ru(bpy)32+/TPrA system can be performed in one chip. We tested gradient pro-oxidation of MCF-7 by ascorbic acid and the synergistic effect of pro-oxidation on doxorubicin. The method achieves high analysis efficiency through a BPE array while simplifying the tedious procedures required by cell culture methods.
研究领域:
生物医学工程
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分析化学
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癌症研究
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Zaiyong Zhang,Jinglong Li,Chunlin Chen,Yuwei Tong,Dehui Liu,Cuizhi Li,Huan Lu,Li Huang,Wanling Feng,Xiaoting Sun
Brain natriuretic peptide (BNP) is considered to be an important biomarker of heart failure (HF) attracting attention. However, its low concentration and short half-life in blood lead to a low-sensitivity detection of BNP, which is a challenge that has to be overcome. In this work, we propose a highly specific, highly sensitive T7 RNA polymerase-assisted clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a system to detect BNP via an electrochemiluminescence (ECL) sensing platform and incorporate Exo III-hairpin and dumbbell-shaped hybridization chain reaction (HCR) technologies. In this detection scheme, the ECL sensing platform possesses low background signal and high sensitivity. Firstly, the T7 promoter-initiated T7 RNA polymerase acts as a signal amplification technique to generate large amounts of RNAs that can activate CRISPR/Cas13a activity. Secondly, CRISPR/Cas13a is able to trans -cleave the surrounding trigger strand to produce DNA1. Thirdly, DNA1 is involved in the co-amplification reaction of Exo III and hairpin DNA, which subsequently triggers a dumbbell-shaped HCR technology. Eventually, a large number of Ru (II) molecules are inserted into the interstitial space of the dumbbell-shaped HCR to generate a strong ECL signal. The CRISPR/Cas13a possesses outstanding specificity for a single base and increased sensitivity. The tightly conformed dumbbell-shaped HCR provides higher sensitivity than the traditional linear HCR amplification technique. Ultimately, the clever combination of several amplification reactions enables the limit of detection (LOD) as low as 3.2 fg/mL. It showed promise for clinical sample testing, with recovery rates ranging from 98.4% to 103% in 5% human serum samples. This detection method offered a valuable tool for early HF detection, emphasizing the synergy of amplification strategies and specificity conferred by CRISPR/Cas13a technology.
研究领域:
生物技术
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分子生物学
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分析化学
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临床诊断
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Hong Ke,Haifeng Sha,Yinfang Wang,Weiwei Guo,Xin Zhang,Zhiming Wang,Chusen Huang,Nengqin Jia
Electrochemiluminescent (ECL) assay has gradually drawn increasing interest in the biomedical analysis. This paper proposed a new methodology for ultrasensitive and facile detection of Alzheimer's disease marker β-amyloid (Aβ) by fabricating a sandwich-type ECL sensing platform. Herein, electrochemiluminescence resonance energy transfer (ECL-RET) was employed to determine Aβ concentration, which can be attributed to the quenching effect from RET between Ru(bpy) 3 2+ and gold nanorods (GNRs) acting as ECL-RET electron donor and acceptor, respectively. In this protocol, mesoporous carbon nanospheres were adopted to immobilize ECL reactant Ru(bpy) 3 2+ and antibody via nafion to acquire the RET donor nanocomposites (MOCs/nafion/Ru(bpy) 3 2+ /antibody), which were tightly interconnected with epoxy group functionalized Fe 3 O 4 nanoparticles . It is of vital importance that GNRs with exquisite rod shape were synthesized and exhibited a typical absorption peak at 650 nm to quench ECL signal of Ru(bpy) 3 2+ effectively. In addition, the ECL emission decreased linearly with the logarithm of Aβ concentration in a wide linear range from 1.0 × 10 −5 to 100 ng/mL with a detection limit of 4.2 × 10 −6 ng/mL. Furthermore, distinctive and desirable properties were verified to declare the promise for being applicable to analyze the Aβ content in real Alzheimer's cerebrospinal fluid samples with satisfactory results.
研究领域:
生物医学工程
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分析化学
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纳米技术
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诊断学
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神经退行性疾病
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DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Huaping Peng,Zhongnan Huang,Weihua Wu,Mingkai Liu,Kaiyuan Huang,Yu Yang,Haohua Deng,Xinghua Xia,Wei Chen
This report outlines a versatile high-performance electrochemiluminescence (ECL) enzyme-linked immunosorbent assay (ELISA) platform, which combines the merits of high-quantum-yield Au nanocluster (AuNC) probe-based ECL technology, the efficient ECL-resonance energy-transfer (ECL-RET) strategy, and highly sensitive and specific ELISA technology. The ECL detection procedure was performed on a recyclable MnO2/AuNC-modified glassy carbon electrode interface by taking advantage of the ECL-RET between the AuNC probe and MnO2 nanomaterials (NMs) to quench the ECL intensity. The etching of MnO2 NMs by the product of ALP-based ELISA recovers the ECL signal. Notably, the ELISA process and the ECL detection procedure in this system are independent. Thus, the ECL-ELISA system can effectively avoid the influence of complex biological samples, and the ECL efficiency of the AuNC probe can be used readily. As demonstrated on TNF-α, because of the abovementioned characteristics, the ECL-ELISA platform presented an extremely wide dynamic range, with a detection limit of 2 orders lower than ELISA. Moreover, the system was also applicable for ultrahigh sensitive detection of various disease-related proteins and able to detect trace biomarkers in real serum samples. Therefore, this multifunctional ECL assay platform is versatile, facile, ultrasensitive, recyclable, and sufficiently straightforward for trace biomarker detection in complex biological samples. This approach not only enriches the foundational study of ECL devices but also greatly expands the potential application of ECL sensors in biological testing and clinical high-throughput diagnosis.
研究领域:
分析化学
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生物化学
·
生物技术
·
临床诊断
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DOI
PubMed
高影响因子
LUMINESCENCE
·
2021
·Vol. 37
·期 1
·58-71
·
IF 2.464
Zheng Chang,Chao Zhang,Binghua Yao
A novel dual-sensitization electrochemiluminescence (ECL) immunosensor for the detection of tumour protein prostate specific antigen (PSA) at trace level using Ru(bpy) 3 2+ -doped chitosan/SiO 2 nanoparticles (Ru(bpy) 3 2+ /chitosan/SiO 2 NPs) as the first signal enhancers was fabricated. Due to chitosan with excellent pore forming capacity, these nanoparticles possess porous structures and better photopermeability, and therefore have higher luminescence efficiencies compared with Ru(bpy) 3 2+ /SiO 2 NPs reported in previous publications. Conversely, chitosan with good biocompatibility and high hydrophilicity was electrochemically decorated onto a Nafion/multiwall carbon nanotubes (Nafion/MWNTs) modified electrode surface and used as the second sensitizing matrix to seize large amounts of prostate specific capture antibody (Ab 1 ). The chitosan-decorated Nafion/MWNTs composites exhibited a 5.5-times higher ECL intensity than the unadorned Nafion/MWNTs films. Also, without additional reagents, such as (3-aminopropyl)triethoxysilane (APTS), the one-step functionalized Ru(bpy) 3 2+ /chitosan/SiO 2 NPs provided a large number of active arms to connect with PSA-detected antibodies (Ab 2 ) through the amino groups in chitosan. After a sandwich immunoreaction, the PSA antigen and Ru(bpy) 3 2+ /chitosan/SiO 2 NPs-labelled Ab 2 were sequentially captured onto the Ab 1 /chitosan/Nafion/MWNTs-modified electrode surface. The ECL signal increases were linearly related to the PSA antigen concentrations and ranged from 0.01 pg·mLl −1 to 10.0 pg·mLl −1 . Under the optimized experimental conditions, the immunosensor displayed excellent sensitivity and selectivity. The detection limit was as low as 3.4 fg·mLl −1 , equivalent to, or better than, those of the reported ECL immunosensors for PSA.
研究领域:
分析化学
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生物传感器
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纳米技术
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生物医学工程
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DOI
PubMed
高影响因子
ACS Sensors
·
2022
·
IF 9.618
Li-Ping Zong,Xiaozhong Chen,Dunru Zhu,Xi-Jie Li,Feng Li,Serge Cosnier,Xue-Ji Zhang,Robert S. Marks,Dan Shan
A novel electrochemiluminescence (ECL) amplification strategy was established aiming to overcome the inherent shortcomings of the current oxygen (O2) coreactant ECL systems. Macrocyclic Schiff base Fe complexes were rationally designed as a novel integrated ECL emitter by iminium linkage between N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and 1,10-phenanthroline-2,9-dicarbaldehyde (PDL) and postmetalation of the macrocyclic Schiff base. Covalently combining luminophore ABEI with a catalytic center endowed the novel ECL emitter with both remarkable redox electrocatalytic properties and significantly enhanced ECL efficiency. The high content of ferrous iron and the dominantly active low-spin Fe state greatly contributed to the inherent catalytic activity for O2 activation. The rational modification of luminophore optimized the spatial distribution and simultaneously shortened the species transport distance of coreactant radicals generated in situ from dissolved O2, resulting in significantly self-enhanced ECL efficiency. Neomycin, which posed a growing threat to aquatic biodiversity and environmental safety, as the model antibiotic was successfully detected with a detection limit of 0.21 pM (S/N = 3), clarifying a promising application prospect of this new luminophore-embedded ECL amplification strategy in biological analysis and environmental monitoring.
研究领域:
分析化学
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环境科学
·
生物分析
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-10
·
IF 5.7
Wang Na,Yang Juan,Luo Zhi,Qin Dongmiao,Wu Yusheng,Deng Biyang
Copper phosphate hybrid nanoflowers (Cu 3 (PO 4 ) 2 HNFs) were demonstrated to produce cathodic ECL emission in the presence of potassium persulfate (K 2 S 2 O 8 ) and then used as a carrier due to their large specific surface area. AgNPs modified on Cu 3 (PO 4 ) 2 HNFs provided more binding sites for immobilizing secondary antibodies and accelerating the electron transfer rate to enhance the ECL signal. In addition, FONDs-Au was used to capture primary antibodies due to its good biocompatibility and large specific surface area. A sandwich electrochemiluminescence (ECL) immunosensor based on copper phosphate hybrid nanoflower/Ag nanoparticle (Cu 3 (PO 4 ) 2 HNFs@Ag) composite and Au NPs-functionalized Fe 2 O 3 nanodendrites (FONDs-Au) was constructed to detect prostate-specific antigen (PSA) in real samples. Under optimal conditions, the constructed sandwich ECL immunosensor was sensitive to PSA with a detection limit of 0.037 pg/mL ( S / N = 3), a linear detection concentration range of 0.0001–50 ng/mL, and a recovery range of 97.33–102.5%. This immunosensor is expected to provide a method to detect PSA or other biomarkers. Graphical Abstract
研究领域:
分析化学
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生物传感器
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纳米技术
·
医学诊断
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Xue Dong,Guanhui Zhao,Li Liu,Xuan Li,Qin Wei,Wei Cao
In this work, Ru(bpy) 3 2+ encapsulated in metal–organic frameworks (MOFs) UiO-67 (Ru(bpy) 3 2+ /UiO-67) as luminophor was easily prepared and firstly applied in constructing an electrochemiluminescence (ECL) immunosensor to efficiently estimate diethylstilbestrol (DES). The competitive method-based ECL immunosensor platform was fabricated by amino-silicon dioxide which possesses large surface area . The poriness of UiO-67 was splendid so that Ru(bpy) 3 2+ could be easily encapsulated. Ru(bpy) 3 2+ /UiO-67 with excellent ECL luminescence signal existed large specific surface area for easily labeled with antibodies. DES competed with bovine serum albumin-diethylstilbestrol (BSA-DES) for binding to antibody-specific sites in the constructed immunosensor. However DES was micromolecule, which was easier to bond to antibodies than BSA-DES. The ECL signal was gradually decreases with the increase of the concentration of DES. Under optimal conditions, the proposed immunosensor exhibited a wide linear range from 0.01 pg mL −1 to 50 ng mL −1 with a low detetion limit of 3.27 fg mL −1 (S/N = 3). The novel fabricated immunosensor with interference immunity and high stability may cause an attractive approach for the other targets determination.
研究领域:
分析化学
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环境科学
·
生物技术
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DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Jingwen Gao,Ziyi Chen,Lebao Mao,Wei Zhang,Wei Wen,Xiuhua Zhang,Shengfu Wang
In this work, an innovative aptasensor based on electrochemiluminescence resonance energy transfer (ECL-RET) from CdTe quantum dots (QDs) to a cyanine dye (Cy5) fluorophore for the determination of Ochratoxin A (OTA) was fabricated. A strong cathodic ECL emission was obtained by the CdTe QDs modified glassy carbon electrode (GCE). After the immobilization with the capture DNA (cDNA) and the sequential hybridization with the probe DNA-modified Cy5 (pDNA, the aptamer of OTA), the ECL signal enhanced obviously through the ECL-RET. Meanwhile, the spectrum- and distance-related ECL enhancement effect was investigated. When the target OTA was in the presence, the pDNA-Cy5 molecules were released from the electrode surface owing to the specific interaction between OTA and aptamer, resulting in an evident decrease of ECL signal. Under optimal conditions, the developed aptasensor displayed the linear response toward OTA in the wide range of 0.0005–50 ng/mL with a low detection limit of 0.17 pg/mL. With the excellent selectivity , stability and repeatability, the strategy provided an efficient and universal method for the sensitive detection of target in practical application.
研究领域:
分析化学
·
生物传感器
·
毒理学
·
食品安全
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Yan Xu,Zhenbo Wang,Caifeng Ding,Xiliang Luo
It is a great challenge to detect the target selectively and accurately in complex biological samples. A ratiometric signal amplification strategy based on antifouling peptide is proposed to detect thrombin (TB) in this work. The electrode is modified with conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and [email protected] Then the bi-functional peptide anchors to the modified electrode and captures the low toxic electrochemiluminescence (ECL) probe. The bi-functional peptide includes a new antifouling sequence (DRDRDRDR) and a TB recognizable sequence (SGRPVLG), while the former can greatly reduce the non-specific adsorption in complex samples and the latter can be cleaved by TB. The new self-enhanced luminescence probe labeled as PAMAM-QDs is composed of low toxic and water-soluble CuInZnS/ZnS quantum dots (CIZS/ZnS QDs) and polyamidoamine (PAMAM) via amide bonds. Concentration of TB can be obtained via the ratio of the signals of ECL QDs /ECL [email protected] accurately and sensitively. The ECL biosensor with the detection line 1.82 fM can work from 10 fM to 1 nM. Thanks to the antifouling property of the designed peptide and the amplification of the new ECL probe, the biosensor can be used for quantifying TB in practical complex samples with high selectivity and sensitivity.
研究领域:
分析化学
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生物传感器
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纳米技术
·
生物化学
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DOI
高影响因子
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