SEPARATION AND PURIFICATION TECHNOLOGY
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2016
·
IF 3.299
Nawshad Muhammad,Yanan Gao,Farasat Iqbal,Pervaiz Ahmad,Rile Ge,Umar Nishan,Abdur Rahim,Girma Gonfa,Zahoor Ullah
In this study the waste fish scales (FS) were dissolved in 1-butyl-3-methylimidazolium acetate ionic liquid to obtain a valuable product of hydroxyapatite (HAp). The HAp was obtained in the yield of 32 ± 2%. The obtained HAp was characterized using Fourier Transform Infrared Spectroscopy (FTIR), Powder X-rays Diffraction (PXRD), Thermal Gravimetric Analysis (TGA), Field Emission Scanning Microscopy (FE-SEM), Energy Dispersive X-rays spectroscopy (EDX), and Brunauer–Emmett–Teller (BET). The results of FTIR and XRD showed the characteristic peaks of the HAp. The thermal degradation temperature of the extracted HAp was relatively high. Furthermore, low weight loss was measured which confirmed the removal of organic part of FS during ionic liquid treatment. The FE-SEM result showed the particles with different morphologies and EDX analysis showed a Ca/P ratio of 1.60 for the extracted HAp. The biocompatibility of the extracted HAp was assessed through MTT cell viability assay using known Human Embryonic Kidney 293 cells (HEK cells) and epidermoid carcinoma cells (A431 cells).
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高影响因子
Food Packaging and Shelf Life
·
2024
·
IF 8.5
Yahong Han,Mingrui Zhou,Jiajie Deng,Cheng Cheng,Zihan Xu,Wenfu Hou,Yang Yi,David Julian McClements,Shuai Chen
Recently, intelligent packaging integrated with natural colorants has garnered interest for its capability to assess the freshness of stored food and delay its spoilage. However, the poor dispersibility and stability of natural pigments limit their application. This study addresses these issues by encapsulating natural pigments in core-shell protein-polysaccharide nanoparticles to enhance their stability and dispersibility. Using curcumin and anthocyanin as examples, pigment-loaded nanoparticles were synthesized using antisolvent precipitation to create zein particles, which were then coated with chondroitin sulfate through electrostatic layer-by-layer deposition. This method produced spherical anionic nanoparticles with high encapsulation efficiency (Curcumin: 91.93 %, Anthocyanin: 85.85 %). These nanoparticles were incorporated into carrageenan-based composite films, which acted as freshness indicators and preservatives. The films' color shifted from green-brown ( L =33.47, a =9.46, b =11.09) to red-brown ( L =22.80, a =13.83, b =2.11) with increasing nanoparticle concentration (from 20 % to 40 % w/v), and their uniform microstructure demonstrated good compatibility with the biopolymer matrix. The films were tested as freshness indicators and preservatives for packaged fish, showing improved freshness detection and preservation effectiveness with higher nanoparticle content. These biodegradable materials are expected to be intelligent packaging solutions for the food sector.
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高影响因子
Water
·
2024
·Vol. 16
·期 17
·2531
·
IF 3
Zhenjiang Yang,Daoquan Zhao,Jinxing Gu,Ran Wu,Bianzhi Liu,Guangqing Yu,Pengsheng Dong,Xiaocheng Huang,Ming Li,Guoxi Li
To investigate the toxic and environmental effects of prometryn, a laboratory experiment was performed on coexistent fish and algae. The body weight and length ofCarassius carassius,Microcystis aeruginosa(M. aeruginosa) densities and water quality were measured continuously for 92 days. It was observed that fish growth was significantly inhibited by prometryn. This can be partly attributed to the adverse effects of prometryn on the antioxidant system of fish; the activities of superoxide dismutase (SOD) and catalase (CAT) in crucian carp were significantly inhibited by prometryn. The growth ofM. aeruginosawas greatly inhibited by prometryn (p< 0.05), and the adverse effects of prometryn onM. aeruginosaindirectly impacted water qualities. The body weight and length ofCarassius carassiusfirst increased and then tended to be stable with increasing electrical conductivity (EC) values; their quantified relationship was established based on the Gompertz and Logistic equations (R2= 0.920–0.989). Based on the above results, we concluded that the toxic effects of prometryn can impact the antioxidant system of fish and algae which in turn affects their growth performance, and have an indirect impact on water quality. The application of equations made it realizable to obtain a more detailed interpretation of the processes involved in these biological–abiotic interactions.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Haotian Zhang,Xiaoying Chen,Hua Rong,Baojia Chen,Junfeng Guang,Qing Pan,Xiaobo Wen,Lijun Ning
Unlike predictability in mammals, the responses of PPARα activation treatment in fish varies between different fish species. Existing results indicated that the varied effects may result from fish feeding habits difference within their varied dietary protein content, while the exact mechanism remains obscure. In this study, we conducted an in vivo 8-week trial of low protein 30 % (LP) or high protein 38 % (HP) diet fed tilapia (8.83 ± 0.02 g) supplemented with or without fenofibrate and in vitro 24-h of BCAA (leucine, valine and isoleucine) treated primary cell trial, aiming to reveal the unknown mechanism. The in vivo results showed that, in LP group, fenofibrate significantly increased serum levels of TG, glucose, AST, ALT, MDA and liver TG ( P < 0.05), while decreased liver, muscle and serum BCAA, liver mitochondria quantity, glucose tolerance ( P < 0.05). Moreover, fenofibrate notably lifted the hepatic bckdha , bckdhb , mff , fis , fas and pparγ expression ( P < 0.05), while decreased genes expression of pgc1α , tfam , parkin , pink , foxo3a, sod2, cat , ir and irs ( P < 0.05). No great impact was found in aco , cyp4a3 and cytb ( P > 0.05). In HP group, fenofibrate improved lipid and glucose metabolism along with unaltered BCAA, increased expression of aco , cytb , cpt1a , pparβ , foxo3a , sod2 , nrf1 , mfn1 and fis ( P < 0.05). In vitro findings revealed that the PGC1α and PPARα could be activated by BCAA, particular for leucine ( P < 0.05). Taken together, we conclude that: 1) increased lipid deposition, lipid peroxidation, liver damage and glucose intolerance in fenofibrate-LP group could be ascribed to decreased BCAA and done through PGC1α/FOXO3, PGC1α/MQC regulation; 2) improved lipid-lowering and glucose tolerance in fenofibrate-HP group would be attributed to the enhanced PGC1α/MQC, organelles proliferation and fatty acid β-oxidation.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shuo Shi,Xiaodan Wu,Ying Wang,Wenxin Li,Hao Zhang,Xinjiang Lou,Xiufang Xia,Weiwei Liang
In this study, hydrophobic sodium alginate/anthocyanin/cellulose nanocrystal indicator films were fabricated by incorporating nanosilica (NS) as a waterproofing layer. The concentrations and formation methods (spraying (S), coating (C), and impregnation (I)) of the NS layer (denoted as NSS, NSC, NSI, respectively) were optimized. The results indicated that the optimum concentration of the NS layer was 5 % at a water contact angle (WCA) 110.5°. Further, Fourier transform infrared spectra showed the presence of Si O Si and Si CH 3 groups in the NSS, NSC, and NSI films, and X-ray diffraction spectra indicated that original structures of these films were disordered. Moreover, the surface morphology, mechanical properties, and light transmission were affected by the NS layer, and the optimal layer was found to be NSI. After 10 days of storage at 100 % humidity, the NSI film exhibited low water vapor adsorption (37.22 g) and permeability (0.1484 g/m·s·Pa·10 −11 ) and a high WCA (110.2°). In addition, the NSI film exhibited a visible color shift with an increasing pH of the buffer solution. A monitoring test of fish freshness showed that the NSI film displayed a distinctive color change corresponding to fish spoilage during 14 days of storage. This indicates that NSI has high potential in indicator film applications.
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PubMed
高影响因子
Nanomaterials
·
2016
·Vol. 6
·期 9
·175
·
IF 2.69
Jia Song,Yiqun Huang,Yuxia Fan,Zhihui Zhao,Wansong Yu,Barbara A. Rasco,Keqiang Lai
Surface-enhanced Raman scattering or surface-enhanced Raman spectroscopy (SERS) is a promising detection technology, and has captured increasing attention. Silver nanowires were synthesized using a rapid polyol method and optimized through adjustment of the molar ratio of poly(vinyl pyrrolidone) and silver nitrate in a glycerol system. Ultraviolet-visible spectrometry, X-ray diffraction, and transmission electron microscopy were used to characterize the silver nanowires. The optimal silver nanowires were used as a SERS substrate to detect prohibited fish drugs, including malachite green, crystal violet, furazolidone, and chloramphenicol. The SERS spectra of crystal violet could be clearly identified at concentrations as low as 0.01 ng/mL. The minimum detectable concentration for malachite green was 0.05 ng/mL, and for both furazolidone and chloramphenicol were 0.1 μg/mL. The results showed that the as-prepared Ag nanowires SERS substrate exhibits high sensitivity and activity.Keywords:SERS;silver nanowires;glycerol;malachite green;crystal violet;furazolidone;chloramphenicol
研究领域:
分析化学
·
食品安全
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高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2020
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IF 4.872
Zhenjiang Yang,Suiliang Huang,Wenwen Kong,Liang Chen,Priakhina Ekaterina,Zobia Khatoon,Muhammad Nabil Ashraf,Waseem Akram
The number of undesirable environmental impacts of fish feed has been reported widely. Although repeated fish feed exposures are more prospective to occur in water, previous studies were mostly conducted as a single exposure of fish feed. In order to fill these gaps, a 40 days incubator experiment was conducted to explore the effects of fish feed addition scenarios during the lag phase with prometryn on both Microcystis aeruginosa growth and concentrations of nutrients. The maximum algae densities in groups of single exposure were 6.0–26.2% and 8.8–74.4% higher than those in groups of double and triple exposures, respectively ( P < 0.05). At the beginning of the experiment, concentrations of nutrients in groups with different feed exposure scenarios were significantly different. The pattern of nutrient limitation showed a transformation from phosphorus limitation to nitrogen limitation generally. Furthermore, the average inhibition rates of algae by prometryn in the case of a single fish feed exposure were 4.6–9.4% lower than those under double exposures, and 22.0–26.8% lower than those under triple exposures ( P < 0.05). In addition, algae growth rates have been developed as a function of concentrations of consumed nutrients ( R 2 = 0.410–0.932). Based on the above results, we concluded that in terms of limiting algae growth multiple low-dosage additions of fish feed were considered as a better addition pattern. By optimizing feed addition scenarios, there is considerable potential to increase the environmental sustainability of aquaculture.
研究领域:
环境科学
·
水生生物学
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高影响因子
AQUATIC TOXICOLOGY
·
2026
·
IF 4.3
Aoxue Wang,Yujia Yan,Yuyao Jiang,Yanru Guan,Zexin Wen,Shengli Zhou,Yanmei Xu,Haisong Cui,Miqi Jin,Wenjun Gui,Shuying Li
Chlorpyrifos (CPF), a commonly applied organophosphate insecticide, is known to disrupt the endocrine systems of aquatic species. However, further research is required to elucidate the molecular mechanisms involved in the reproductive toxicity of fish. In the present study, zebrafish were exposed to environmentally relevant concentrations of CPF (1, 10, and 50 μg/L) for a 21-day period. Within the concentration range at which chlorpyrifos significantly inhibits the activity of acetylcholinesterase in zebrafish, there is a significant decrease in reproductive capacity of 31.65%. The RNA-seq analysis has revealed that processes such as transporter activity, reproduction, and reproductive function are affected. Elevated FSH and LH levels in CPF-exposed females were observed at concentrations of 10 μg/L and 50 μg/L. Furthermore, the CPF treatment resulted in the upregulation of esr2b, hsd3b1, fshr, gnrh2, vtg1 , and vtg2 , while cyp19a1a was found to be downregulated. Co-exposure to cetrorelix acetate, a potent GnRH receptor antagonist, has been demonstrated to normalize gene expression and restore E 2 levels. These results suggest that CPF-induced reproductive toxicity involves crosstalk between cholinergic signaling and the HPG axis. These findings underscore the complex toxicity and ecological risks associated with CPF.
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高影响因子
FOOD CONTROL
·
2025
·
IF 6.3
He Wang,Yang Huang,Xiaofang Liu,Zhuolin Liu,Kunpeng Qian,Li Li
Currently, biodegradable membranes are popular but lack biological activity. Inspired by the natural mussel, based on the preparation of amyloid membranes by phase transition lysozyme (PTL), the active amyloid membrane was successfully prepared by grafting tannic acid (TA) with lysozyme and mixed ε-poly-L-lysine (ε-PL). FTIR demonstrated that lysozyme successfully grafted TA, and the conformation after grafting was simulated by molecular docking. The DPPH free radical scavenging rate of the final obtained ε-PL-TA-PTL coating (ε-T-PTL) was 41.32 %, the inhibition rates of S. aureus and E. coli were significantly improved to 99.5 % and 99.9 %. Dipping method was applied to the preservation of snakehead fish, ε-T-PTL shown excellent preservation properties in delaying snakehead fish oxidation, inhibiting the protein degradation and microbial growth, finally extend the shelf life by 40 %. Thus, ε-T-PTL coating is expected to become a convenient and efficient new food packaging material.
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高影响因子
JOURNAL OF TEXTURE STUDIES
·
2025
·Vol. 56
·期 2
·e70016
·
IF 2.5
Kaixuan Wang,Shengnan Zhan,Jianyuan Yang,Qiaoming Lou,Tao Huang
In this study, environmental stability, rheological properties, and structural characterization of co-stabilized emulsions using fish gelatin (FG) and tea saponin (TS) were investigated. The results demonstrated that the addition of TS significantly enhanced the emulsifying properties of FG, and the FG-TS 0.1% emulsion had the smallest particle size. TS and FG co-stabilized emulsions provided resistance to salt and high temperatures. Optical microscopy and CLSM showed that the addition of TS made FG more effectively adsorb at the oil–water interface, leading to the formation of more uniform oil droplet sizes. Additionally, the addition of TS increased the viscosity of FG emulsions, which reduced emulsion flocculation. Results of intrinsic fluorescence, FTIR, and surface hydrophobicity revealed that the addition of TS altered the secondary structure of FG, enhancing surface hydrophobicity and improving emulsification. In conclusion, the moderate addition of TS significantly enhanced the emulsification and rheological properties of FG, suggesting new potential applications for FG in various industries.
研究领域:
食品科学
·
生物化学
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PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Shiyao Wang,Shanshan Liu,Xinxin Yang,Shihao Zhang,Xing Li,Lixin Zhang,Yisheng Chen
A novel colorimetric paper-sensor (CPS) in this research was fabricated by functionalizing filter paper with mulberry anthocyanin to monitor the freshness of packaged carp and milk. A 2D printer was applied to distribute uniformly mulberry anthocyanin onto the filter paper to form 10 × 10 mm 2 squares, whose colors had a response to ambient pH values. The obtained CPS demonstrated satisfactory stability and reliable color-rendering capacity at pH 2–12. The correlation between color variation and sample freshness was quantitatively evaluated. The application of CPS in detecting the freshness of carp and milk at 25 °C was further studied, whose results evidenced that CPS had a significant color variation when ΔE was greater than 5. Additionally, its color turned from red-violet to gray when TVB-N of carp was greater than 20 mg/mL, implying that there was a strong positive correlation between TVB-N and color variation (R = 0.515). Finally, quantitative analysis about color was conducted by the color recognition software of smartphone. The results indicated when S value (the newly defined color calculation method) was less than 50 or R value was greater than 120, milk and fish were rotten. Consequently, 2D printer could be a promising tool to fabricate intelligent packaging materials enabling monitor visually freshness.
研究领域:
食品科学
·
包装技术
·
生物化学
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高影响因子
International Journal of Polymer Science
·
2017
·
IF 1.077
Yunzhen Yao,Ding Ding,Hongyuan Shao,Qifan Peng,Yaqin Huang
Fish gelatin-chitosan edible films with D-limonene were successfully prepared, which exhibited exceptional mechanical properties and antimicrobial activity. It has been demonstrated that water-soluble chitosan, fish gelatin, and D-limonene could be a candidate precursor to prepare low cost and high-performance edible food packaging material. The results showed that D-limonene in the films could effectively resist the penetration of light and water because of its hydrophobicity. Moreover, the elongation at break (EAB) increased with the addition of D-limonene, which indicated that D-limonene served as a strong plasticizer for the film. Microscopic characterization showed that D-limonene was uniformly distributed in the as-prepared film. And we found that the film exhibited strong antibacterial activity against Escherichia coli (E. coli). All the results indicate that the as-prepared film could be a promising food packaging.
研究领域:
材料科学
·
食品科学
·
生物技术
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高影响因子
危险品化学品经营许可证（带存储）