JOURNAL OF MOLECULAR STRUCTURE
·
2019
·
IF 2.12
Xuanping Tan,Qin Li,Jidong Yang
A simple and sensitive method for detecting LFO was developed based on electron transfer mechanism quenched the fluorescence of GSH-CdTe QDs. LFO, as an effective electron acceptor , is added into the QDs solution for electron transfer, the transfer of electrons from the conduction band of QDs to LFO prevents the recombination of free electrons and valence band holes of QDs, thus causing the fluorescence quenching of QDs. Under the optimal experimental conditions, the fluorescence quenching degree of QDs has a good linear relationship with the concentration of LFO within the range of 0.73–7.30 μg mL −1 , the correlation coefficient is 0.9978, and the detection limit is 1.53 ng mL −1 . In addition, the reaction mechanism and experimental conditions were investigated, and the method was successfully applied to the detection of LFO in milk and serum samples.
研究领域:
分析化学
·
食品安全
·
生物医学
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产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2014
·Vol. 25
·期 23
·235101
·
IF 3.672
Xiaoqin Chen,Yajun Tang,Bing Cai,Hongsong Fan
A novel quantum dots-based multifunctional nanovehicle (DOX-QD-PEG-FA) was designed for targeted drug delivery, fluorescent imaging, tracking, and cancer therapy, in which the GSH–CdTe quantum dots play a key role in imaging and drug delivery. To exert curative effects, the antineoplastic drug doxorubicin hydrochloride (DOX) was loaded on the GSH–CdTe quantum dots through a condensation reaction. Meanwhile, a polyethylene glycol (PEG) shell was introduced to wrap the DOX-QD, thus stabilizing the structure and preventing clearance and drug release during systemic circulation. To actively target cancer cells and prevent the nanovehicles from being absorbed by normal cells, the nanoparticles were further decorated with folic acid (FA), allowing them to target HeLa cells that express the FA receptor. The multifunctional DOX-QD-PEG-FA conjugates were simply prepared using the 'one pot' method. In vitro study demonstrated that this simple, multifunctional nanovehicle can deliver DOX to the targeted cancer cells and localize the nanoparticles. After reaching the tumor cells, the FA on the DOX-QD-PEG surface allowed folate receptor recognition and increased the drug concentration to realize a higher curative effect. This novel, multifunctional DOX-QD-PEG-FA system shows great potential for tumor imaging, targeting, and therapy.
研究领域:
生物医学工程
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肿瘤学
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 7
·981
·
IF 4.7
Chang Liu,Jianing Wang,Dan Hong,Zhou Chen,Siting Li,Aijin Ma,Yingmin Jia
Rumexpatientia L.×Rumextianshanicus A. Los(RRL), known as “protein grass” in China, was recognized as a new food ingredient in 2021. However, the cultivation and product development of RRL are still at an early stage, and no peptide research has been reported. In this study, two novel antioxidant peptides, LKPPF and LPFRP, were purified and identified from RRL and applied to H2O2-induced HepG2 cells to investigate their antioxidant properties. It was shown that 121 peptides were identified by ultrafiltration, gel filtration chromatography, and LC-MS/MS, while computer simulation and molecular docking indicated that LKPPF and LPFRP may have strong antioxidant properties. Both peptides were not cytotoxic to HepG2 cells at low concentrations and promoted cell growth, which effectively reduced the production of intracellular ROS and MDA, and increased cell viability and the enzymatic activities of SOD, GSH-Px, and CAT. Therefore, LKPPF and LPFRP, two peptides, possess strong antioxidant activity, which provides a theoretical basis for their potential as food additives or functional food supplements, but still need to be further investigated through animal models as well as cellular pathways.
研究领域:
食品科学
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生物化学
·
药理学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2015
·Vol. 182
·期 11
·2009-2017
·
IF 3.741
Hao Chenxia,Liu Shaopu,Liang Wanjun,Li Dan,Wang Linlin,He Youqiu
We report on a simple, sensitive and regenerable fluorescent nanoprobe for Zn(II) ion. It is based on the use of glutathione capped CdTe quantum dots (GSH-CdTe Q-dots). The bright fluorescence of these Q-dots is quenched on addition of diethylenetriaminepentaacetic acid (DTPA) due to the binding of DTPA to GSH. If, however, Zn(II) is added, it will bind DTPA and detach it from the surface of the Q-dots, this resulting in the fluorescence recovery. Under optimum conditions, the intensity of the restored fluorescence is proportional to the concentration of Zn(II) in the 0.48 to 90 μmol · L −1 range, with a limit of detection of 0.14 μmol · L −1 . The nanoprobe was applied to the determination of Zn(II) in spiked tap water and river water and gave satisfactory results. The findings were also applied to design a molecular logic gate where DTPA acts as the first input to the system by quenching the fluorescence of the GSH-CdTe Q-dots. Zn(II) acts as the second input and causes the detachment of DTPA from the Q-dots and a restoration of fluorescence. This system therefore represents a new IMP (IMPLICATION) logic gate.
We describe a fluorescent nanoprobe for Zn(II) based on quantum dots, and its use in an IMP molecular logic gate. The nanoprobe was successfully applied to the determination of Zn(II) in spiked tap water and river water.
研究领域:
分析化学
·
纳米科技
·
生物传感
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产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2013
·
IF 2.144
Suyan Ying,Shumin Cui,Weiping Wang,Jiuju Feng,Jianrong Chen
A simple and sensitive method for detecting diprophylline (DPP) was developed based on the fluorescence quenching of glutathione-capped CdTe quantum dots (GSH–CdTe QDs) by using diprophylline in a KH 2 PO 4 –Na 2 HPO 4 medium. Parameters affecting the quenching efficiency, including types and pH of buffer solutions as well as temperature, reaction time, adding sequence, and interfering substances, were investigated and optimized. In optimum conditions, the calibration plot of the quenched fluorescence intensity F 0 / F with a DPP concentration range of 1.67×10 –6 mol L −1 to 1.33×10 –5 mol L −1 was linear. The detection limit (with signal to noise ratio of 3) for DPP was 2.24×10 –7 mol L −1 . The proposed method was successfully applied for detecting DPP in human serum. The recovery of the method was in the range of 87.41% to 117.94%. Finally, the possible quenching mechanism of GSH–CdTe QDs and DPP was also discussed.
研究领域:
分析化学
·
药学
·
生物医学工程
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产品关联
DOI
高影响因子
IUBMB LIFE
·
2021
·Vol. 73
·期 9
·1180-1190
·
IF 3.885
Jing Wang,Tao Wang,Yang Zhang,Jiaqi Liu,Jie Song,Yanlong Han,Lihong Wang,Shuang Yang,Lili Zhu,Rui Geng,Weimin Li,Xiaoguang Yu
The induction of ferroptosis is considered a new strategy for cancer treatment. Cytoplasmic polyadenylation element binding protein 1 (CPEB1) is a post-transcriptional regulatory factor, whose low expression has been reported to link to the enhanced metastasis and angiogenesis of gastric cancer (GC). In this study, to explore the role of CPEB1 in ferroptosis, GC cells with overexpressed or silenced CPEB1 expression were treated with erastin, a classic ferroptosis inducer. The results showed that erastin dose-dependently decreased the viability of four GC cell lines (AGS, SNU-1, Hs-746 T, and HGC-27), suggesting that ferroptosis could be triggered in these GC cells. Interestingly, HGC-27 cells overexpressing CPEB1 were more sensitive to erastin, generated more lipid reactive oxygen species (ROS) and malondialdehyde (MDA), and their glutathione peroxidase 4 (Gpx4) expression and GSH content were reduced. Contrarily, CPEB1-silenced AGS cells were more resistant to erastin. Mechanically, we demonstrated that CPEB1 overexpression reduced the expression of twist1, an inhibitor of activating transcription factor 4 (ATF4), thereby activating the ATF4/ChaC Glutathione Specific Gamma-Glutamylcyclotransferase 1 (CHAC1) pathway (CHAC1, a molecule known to induce GSH degradation). Furthermore, re-expression of twist1 in GC cells impaired the effects of CPEB1 overexpression in presence of erastin. Additionally, similar to the in vitro results, the growth-inhibiting effects of erastin on GC xenografted tumors were also augmented by CPEB1 overexpression in vivo. Collectively, we demonstrate that CPEB1 facilitates erastin-induced ferroptosis by inhibiting twist1.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Advances
·
2023
·
IF 0
Yiqing Liu,Peiyong Guo,Xinhao Dong,Yina Xu,Dan Li,Hua Zheng,Jianhong Liao
Here, we combined reversible addition-fragmentation chain transfer (RAFT) polymerization and amide coupling reaction to develop a novel drug-polymer conjugate using poly(AMA- co -IMMA)-b-poly(OEGMA) (termed as PAIPO) as nanocarriers. In order to enhance cellular uptake and obtain subsequent endo/lysosomal escape capacity, the dual-drugs-conjugated prodrug was then coupled with 2,3-dimethylmaleimide (DA) moieties and implanted with imidazolyl groups, respectively. Paclitaxel (PTX) was conjugated to PAIPO via 3,3′-dithiodipropionic acid (DPA) to construct a GSH-responsive moiety, while doxorubicin (DOX) was conjugated to PAIPO via 4-formyl benzoic acid to construct a pH-responsive moiety, which synergistically enabled a synchronized and precise drug delivery. The micelles self-assembled from DOX/ [email protected] DA showed an ideal average diameter (163.2–178.3 nm), contributing to passive targeting by the EPR effect. Moreover, a switch of the surface Zeta potential of micelles from steady negatively charged (− 9.74 ± 0.54 mV) at pH 7.4 to positively charged (+ 6.33 ± 1.25 mV) at pH 6.5, facilitated the long blood circulation and cellular endocytosis of micelles, respectively. More importantly, in vitro studies confirmed that DA M(DOX n /PTX) exhibited a strong synergism against tumor cells, and under slightly acidic conditions (pH 6.5), the combination index (CI) values for DA M(DOX 1 /PTX) on HeLa and Skov-3 cells were estimated to be 0.47 and 0.49 (previous to be 0.50 and 0.56 at pH 7.4), respectively. And in vivo results showed effective tumor accumulation potential, remarkable biosafety, and biocompatibility. Combined, such synchronized delivery approach based on multi-responsive micelles might potentiate the efficacy of combination chemotherapy in clinical cancer treatment.
研究领域:
药物递送系统
·
肿瘤学
·
生物材料
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产品关联
DOI
PubMed
JOURNAL OF CONTROLLED RELEASE
·
2025
·
IF 11.5
Jian Xiao,Nana Feng,Qiushi Li,Xianghui Cao,Qingqing Huang,Biyu Zhou,Zhenrui Fan,Liping Wei,Yang Liu
Ferroptosis is gaining attention as a potential cancer immunotherapy strategy, as it can stimulate antitumor responses by enhancing dendritic cell (DC) activation and the infiltration of cytotoxic T cells (CTLs). However, cancer cells often develop resistance to ferroptosis, reducing the effectiveness of existing treatments. This study demonstrates a novel mitochondria-targeted ferroptosis inducer, designated mitoFePDA@R, which is engineered to achieve a “closed-loop” cancer immunotherapy strategy of ferroptosis induction, antitumor immune activation, and ferroptosis enhancement. In this strategy, mitoFePDA@R is designed to release Fe 2+ and the mitoGPX4 inhibitor RSL3 within tumor mitochondria, thereby effectively inducing ferroptosis and activating strong antitumor immune responses. Additionally, interferon γ ( IFN-γ) released from CTLs inhibits GSH synthesis, which further enhances the ferroptosis sensitivity of tumor cells to form a “closed-loop” strategy. In vitro studies indicated that mitoFePDA@R induced strong ferroptosis in tumor cells by accumulating lipid peroxides (LPO) in mitochondria (which lacks mitochondria targeting). Animal studies confirmed that mitoFePDA@R effectively triggered ferroptosis and activated subsequent antitumor immune responses, leading to significant tumor growth inhibition. This provides a viable and effective strategy for ferroptosis-associated cancer immunotherapy.
研究领域:
肿瘤学
·
免疫学
·
细胞生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2021
·
IF 5.875
Yue Yang,Kaiqing Yun,Yunjian Li,Ling Zhang,Wenxuan Zhao,Zhihong Zhu,Baocheng Tian,Fen Chen,Weisan Pan
Integration of multiple therapies into one nanoplatform holds great promise to overcome the shortcomings of traditional single-modal therapy and achieve favorable antitumor efficacy. Herein, we constructed a dual receptor-targeting nanomicelle system with GSH-responsive drug release for precise fluorescence imaging and superior chemo-phototherapy of cancer. The synthetic amphiphilic hyaluronic acid derivative (FHSV) could self-assemble into nanomicelles in aqueous media. Then, paclitaxel (PTX) and photosensitizer IR780 iodide (IR780) were co-loaded into the micelles by a simple dialysis method. The resulting IR780/PTX/FHSV micelles with a particle size of 150.2 ± 6.9 nm exhibited excellent stability, GSH-responsive drug release and good photothermal/photodynamic efficacy. Once accumulated at the tumor sites, IR780/PTX/FHSV micelles efficiently entered tumor cells through receptor-mediated endocytosis and then rapidly release PTX and IR780 under GSH-rich tumor microenvironment . Upon NIR laser irradiation, IR780 produced local hyperthermia and sufficient reactive oxygen species to promote tumor cells apoptosis and necrosis. The results of in vitro and in vivo experiments consistently demonstrated that compared with single chemotherapy and phototherapy, the chemo-phototherapy could more efficiently kill tumor cells by synergistic antitumor effect. Therefore, our study provides a novel and efficient approach for multimodal treatment of malignant tumor.
研究领域:
药剂学
·
肿瘤学
·
生物医学工程
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DOI
PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Shuyun Zhu,Lei He,Fang Zhang,Mengyuan Li,Shulin Jiao,Yan Li,Mengyuan Chen,Xian-En Zhao,Hua Wang
A highly sensitive fluorimetric assay has been developed for the evaluation of glutathione reductase (GR) activity and the screening of its inhibitors by using carbon quantum dots (CQDs) as the signal reporter. The detection mechanism is based on the following facts: (1) the fluorescence of CQDs can be quenched by Hg(II) through the strong CQDs-Hg(II) coordination; (2) GR can catalyze the reduction of oxidized glutathione (GSSG) into reduced glutathione (GSH), so that the fluorescence recovery of CQDs would take place resulting from the strong GSH-Hg(II) interaction; (3) GR can lose its catalytic reduction of GSSG in the presence of its inhibitors, which will inhibit the recovery of the quenched fluorescence of CQDs. The developed CQDs-based fluorimetric method can facilitate the sensitive evaluation of GR activity in the range of 0.10–2.0 mU mL −1 with a detection limit of 0.050 mU mL −1 . In addition, other kinds of enzymes like myoglobin , thrombin, alcohol dehyrogenase, amylase, pepsin, and trypsin could show no significant effects on the evaluation of GR activity. This work may expand the biological applications of CQDs as the fluorescent probes with low cost, easy preparation, and high photostability.
研究领域:
生物化学
·
分析化学
·
药物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2026
·
IF 6
Yawen Yao,Min Zhang,Sai Xu,Guoping Wu,Xinwang Meng,Juan Li,Hua Yu
A chondroitin sulfate-functionalized GSH-responsive lipid-nanoparticle (CSD@DNLP) was developed for co-delivering doxorubicin (DOX) and nifuroxazide (NIF) in metastatic melanoma treatment. • Chondroitin sulfate-functionalization improved cellular uptake through targeting CD44-receptors and enhanced drugs release in tumor-mimic environment. • Synergistic effects were observed in tumor growth inhibition and metastasis repression through NIF-mediated STAT3 phosphorylation blockade and DOX-induced DNA damage. • CSD@DNLP demonstrated a promising strategy in metastatic melanoma. Tumor metastasis results in high mortality rate in patients with advanced melanoma. The hyperphosphorylation of signal transducer and activator of transcription 3 (STAT3) is closely associated with the proliferation and metastasis of melanoma. Here, we proposed a lipid-nanoparticle (CSD@DNLP) derived from the chondroitin sulfate (CS)-conjugated deoxycholic acid (DOCA) polymer (CS-ss-DOCA, CSD) for the co-delivery of doxorubicin (DOX) and STAT3 inhibitor nifuroxazide (NIF) in metastatic melanoma treatment. The superior affinity between CS and overexpressed CD44 receptors in B16F10 cells resulted in 3.3-fold enhancement of cellular uptake compared to free DOX. CSD@DNLP maintained structural stability in physiological conditions, while achieving 75.15% cumulative release within 48 h under a tumor-mimic concentration of glutathione (GSH, 20 mM). Synergistic effects were observed through NIF-mediated STAT3 phosphorylation blockade and DOX-induced DNA damage, resulting in 3.2-fold enhancement in apoptotic cells versus free drugs. In lung metastasis mice, CSD@DNLP suppressed metastatic nodule formation (36% of free DOX + NIF) via dual downregulation of matrix metalloproteinases-2 (MMP-2) and metalloproteinases-2 (MMP-9). This CS-functionalized GSH-responsive lipid-nanoparticle demonstrates a promising strategy in metastatic melanoma. Download: Download high-res image (162KB) Download: Download full-size image
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DOI
PubMed
高影响因子
MATERIALS & DESIGN
·
2026
·
IF 8.2
Yunxin Ye,Weian Wu,Yaping Chen,Jiyuan Zou,Ting Lin,Bo Wu,Tao Luo,Li Yang,Lvhua Guo
BCA with modified molecular skeleton exhibits potent GSH scavenging capacity. • M@PB nanozyme was designed with Pd NPs and BCA co-loaded on MPDA NPs. • M@PB achieves adaptive ROS regulation via microenvironmental pH changes. • M@PB can effectively promote the healing of MRSA-infected wounds. Nanozymes show great potential in reactive oxygen species (ROS)-based antibacterial therapy. However, bacterial glutathione (GSH)-mediated ROS scavenging and tissue damage caused by residual ROS after sterilization remain challenges. Herein, we report a ROS-adaptively regulated M@PB, which is constructed by co-loading multienzyme Pd nanoparticles and a GSH scavenger α-bromocinnamaldehyde (BCA) onto mesoporous polydopamine (MPDA). The M@PB exhibits pH-switchable enzyme-like activities: under acidic conditions, it exerts peroxidase (POD)-like activity to generate ROS for efficient bacterial elimination; as bacteria are eradicated and the local pH rebounds, it switches to catalase (CAT)-like activity to scavenge residual ROS and produce oxygen, thereby accelerating cell migration. Furthermore, backbone-modified BCA exhibits strong GSH-scavenging activity; its acid-triggered release from M@PB further disrupts bacterial antioxidant systems, driving greater oxidative stress to accelerate the antibacterial-to-healing progression. Experiments demonstrate that M@PB exhibits potent antibacterial and antioxidant properties with favorable biosafety, and can effectively promote the healing of methicillin-resistant Staphylococcus aureus ( MRSA )-infected wounds. This work provides a pH-Switchable nanoplatform that enables flexible regulation of ROS, offering a robust strategy for the care of wounds infected with drug-resistant bacteria. Download: Download high-res image (177KB) Download: Download full-size image
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DOI
高影响因子
危险品化学品经营许可证（带存储）