ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
·
IF 6.1
Changsheng Ma,Sihao Chen,Xiaoyu Shi,Lianyang Bai,Hongmei Li-Byarlay,Zuren Li
Glyphosate (GLY) stands as one of the most extensively used pesticides globally. Although several studies have investigated the effects of sublethal doses of GLY on the gut microbiota of bees, limited information is available on how residual concentrations of GLY in bee food affect Apis cerana (Asian honey bees) and Apis mellifera (European honey bees) at different time points. To explore these effects, we employed 16S rRNA Illumina sequencing technology to evaluate the consequences of chronic GLY exposure (0.7 mg/kg, GLY residual in beebread) on the gut microbiome of A. cerana and A. mellifera on days 3 and 12 of the exposure period. Even though our findings revealed no significant difference in the microbiota abundance between control bees and GLY-treated bees on the 3rd day, GLY-treated bees exhibited 55.56 % abundance of the genus Snodgrassella (a core gut bacterium) and 4.54 % of the genus Commensalibacter (a non-core gut bacterium) compared to control bees in A. cerana and A. mellifera , respectively. In addition, our results showed that a noticeable trend indicated that the gut of A. mellifera was more susceptible to GLY than that of A. cerana . Considering the vital role of gut microbiota in the health of honey bee colonies, our results suggest that 0.7 mg/kg of GLY are likely to have adverse effects on the health of honey bees.
研究领域:
环境毒理学
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微生物生态学
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养蜂学
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农药科学
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昆虫微生物学
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Food Chemistry-X
·
2025
·
IF 8.2
Hui Ma,Anqi Wen,Tianmin Wang,Qianhong Li,Zihan Li,Weijie Lan,Kang Tu,Xiaoman Ji,Leiqing Pan
A highly sensitive and rapid surface-enhanced Raman spectroscopy (SERS) method was developed for glyphosate (GPH) detection in rice by combining carbon disulfide (CS 2 )-mediated derivatization with an optimized silver nanoparticle (AgNP) aggregation strategy. GPH was converted into a SERS-active thiazole derivative through pH-controlled reactions. An adsorption-first strategy (analyte pre-adsorption before aggregation) enhanced signals by 64.78 % at 1 min and maintained 11.83 % higher intensity after 6 min versus conventional aggregation-first methods, due to optimized hotspot positioning. The method achieved a detection limit of 0.13 μmol/L (signal-to-noise ratio = 3), below European Union regulatory limits, with excellent reproducibility (RSD = 8.39 %), high selectivity against amino acid interferents, and high time efficient (22 min total analysis). Moreover, spike-recovery tests in rice yielded 80.82 % − 129.62 % recovery, providing a robust platform for detection of trace GPH in complex food matrices. Consequently, this work establishes a time-efficient and cost-effective alternative to conventional chromatography, which facilitates rapid on-site monitoring.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Zi-han Chang,Xing-yu Long,Jing-wen Gu,Mei Zhang,Jiang-qun Zhang,Ting-mei Wang,Yan Zhang,Hui Xie,Yang Li,Pin-hua Xia
Based on the unique affinity interaction between amino (NH 2 ) and metal oxides (MOs) with phosphate groups (PO 4 3− ), herein, Fe 3+ , Fe 2+ and Sm 3+ were respectively usedas metal ion source, polyethyleneimine (PEI) as functional monomer, together with ammonia as precipitant to prepare a novel PEI-functional magnetic rare earth samarium metal oxide composite nano material (PEI/Fe 3 O 4 -Sm 2 O 3 , denoted as PFS) using a “one pot method” for the adsorption study of simulating high concentration glyphosate (GLY) in waterbodies emergency pollution. Consequently, the PFS adsorbent was characterized and analysed successfully using techniques such as SEM, TEM, EDS, BET, XRD, XPS, VSM and so on. A single-factor adsorption experiment was performed to explore how factors like the initial concentration of GLY ( C 0 ), solution pH, adsorbent dosage ( m ), and oscillation time affect PFS's adsorption of GLY. Under the optimized conditions, the adsorption of GLY by PFS could reach up to 92.3 % with a maximum saturated adsorption capacity Q max = 178.5 mg/g. Furthermore, PFS had a certain recyclability after 4 cycles adsorption-desorption of GLY. In addition, the Langmuir model ( R 2 = 0.962) could be better described the adsorption process as examined by isothermal adsorption modelling. The kinetic model fit the pseudo-secondary dynamics well ( R 2 = 0.999), and the adsorption process was spontaneous and endothermic. Besides, we the possible mechanisms of GLY adsorption by PFS were preliminarily explored, which was synergistically promoted by three main adsorption mechanisms, including electrostatic interaction and hydrogen bonding as well as covalent bonding. Morever, PEI-functional magnetic nano-Sm 2 O 3 with high affinity was first used as an adsorbent for GLY due to the synergistic interaction between Sm 2 O 3 and -HPO 3 − , NH 4 + and -HPO 3 − /-COO − . Finally, PFS was used to remove residual GLY in real world water environment (Baiyun lake, Siya river and farmland water) using a convenient and rapid magnetic separation process. The adsorption and removal of GLY in adding standard samples experiments on different substrate waters were above 95 % and could avoid the adverse effects of GLY residues on the aquatic environment. The above results indicated that PEI/Fe 3 O 4 -Sm 2 O 3 , which is a new and excellent nano adsorbent, exhibits a excellent adsorption capacity for GLY, demonstrating a large potential for practical water applications, especially for urgently water bodies polluted with high concentrations of GLY.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2025
·Vol. 26
·期 20
·9887
·
IF 4.9
Qiuying Lu,Dongmiao Zhai,Yaxian Wu,Yihu Mao,Golam Jalal Ahammed,Xinzhong Zhang,Jingbo Yu,Xin Li
Glyphosate is one of the most widely used herbicides in agricultural, horticultural, and urban environments. However, its residue accumulation and oxidative damage pose serious threats to crop health and food safety. In this study, we evaluated the potential of epigallocatechin gallate, a natural polyphenol derived from tea, to alleviate glyphosate-induced stress in melon (Cucumis meloL.). LC-MS/MS analysis revealed that EGCG significantly reduced glyphosate residues in plant tissues. Transcriptome analysis indicated that glyphosate induced extensive transcriptional reprogramming, activating genes involved in detoxification and antioxidant defense. Co-treatment with glyphosate and EGCG partially mitigated this stress response and redirected gene expression toward secondary metabolic pathways, particularly flavonoid and phenylalanine biosynthesis. Under herbicide stress, EGCG restored the transcription of key flavonoid biosynthetic genes, includingPAL,C4H,CHI, andOMT. Meanwhile, EGCG also modulated the expression ofAPX,SOD, andGST, suggesting a selective effect on antioxidant systems. Co-expression network analysis identified key hub genes associated with oxidative stress and flavonoid metabolism. These findings demonstrate the dual regulatory role of EGCG in suppressing acute oxidative stress while enhancing metabolic adaptability, highlighting its potential as a natural additive for reducing herbicide residues in fruit crops.
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JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Fen Liu,Wei Xiang,Chen Wang,Yongchun Zhao,Tao Zhou,Mingjie Huang,Xiaohui Wu
High-concentration glyphosate (Gly) wastewater presents a formidable remediation challenge due to its recalcitrance and the inherent risk of generating toxic intermediates like aminomethylphosphonic acid (AMPA). Herein, we report a facile “waste-treats-waste” strategy utilizing a self-accelerated Fe(II)/O 2 system that operates efficiently under neutral pH, effectively circumventing the acidic constraints of traditional Fenton processes. This system achieved a 57% Gly removal efficiency (initial concentration 0.5 g/L) within 60 min, with a kinetic rate constant ( k obs = 0.029 min −1 ) significantly outperforming conventional oxidation technologies. Mechanistic investigations reveal that Gly acts as a suicide ligand, forming an inner-sphere Fe(II)-Gly complex that lowers the Gibbs free energy barrier, thereby rendering O 2 activation thermodynamically favorable. The degradation proceeds via a dual-pathway mechanism, synergizing hydroxyl radical (•OH) attack (∼40%) with a dominant intramolecular ligand-to-metal charge transfer (LMCT) process (∼60%). Crucially, density functional theory (DFT) calculations elucidate that Fe(III) coordination induces a regioselective weakening of the C–N bond adjacent to the phosphonate group. This steric and electronic modulation steers the reaction pathway toward the preferential formation of benign glycine, successfully suppressing the generation of highly toxic AMPA. Bioassays using Tetrahymena thermophila confirmed that this glycine-selective pathway optimization significantly mitigates the acute toxicity of the effluent, validating the system as a sustainable and eco-safe solution for herbicide wastewater treatment.
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Environmental Chemistry Letters
·
2019
·Vol. 17
·期 3
·1421-1428
·
IF 4.617
Zhang Zhengfang,Ouyang Zhuozhi,Yang Jingjing,Liu Yanjun,Yang Chen,Dang Zhi
The increasing presence of pesticides, pharmaceuticals and plasticizers in environmental media is threatening public health and ecosystems. Remediation of contaminated waters by Fenton-like photoreactions to degrade pollutants is considered as sustainable because this method mimics natural attenuation and involves safe iron oxides and oxalate. However, knowledge about the degradation efficiency of organic pollutants is scarce, notably in the presence of minerals. Here, we tested the degradation of the tetracycline antibiotic, the diethyl phthalate plasticizer, and the glyphosate herbicide using combinations of visible light, goethite (FeO(OH)), and oxalate. Pollutant residues were also extracted from the precipitate at the end of a reaction. Results show that pollutant removal, including adsorption and degradation, reached 99.7% for tetracycline, 87.1% for diethyl phthalate, and 83.3% for glyphosate using a goethite/oxalate/visible light system. The corresponding degradability was 99.5% for tetracycline, 87.1% for diethyl phthalate, and only 39.7% for glyphosate. These findings show that, whereas tetracycline and diethyl phthalate are well degraded, a major proportion of glyphosate remains adsorbed on goethite. As a consequence, glyphosate levels and toxicity may have been underestimated in previous investigations since glyphosate and glyphosate residues may be further desorbed from minerals and reenter aquatic and biological media.
研究领域:
环境科学
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污染修复
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生态毒理学
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RSC Advances
·
2019
·Vol. 9
·期 8
·4635-4643
·
IF 3.049
Xiufang He,Zhansheng Wu,Yongtao Xue,Zhenzhen Gao,Xia Yang
Graphitic carbon nitride (g-C3N4) has been considered to be a promising metal-free photocatalyst, although the high recombination rate of charge carriers and poor absorption of visible light have limited its applications. In order to overcome these problems, an interlayer composite photocatalyst that comprised β-cyclodextrin (β-CD), oxygen-doped C3N4 (O-C3N4) and molybdenum disulfide (MoS2) was successfully constructed for the highly enhanced photodegradation of glyphosate in this study. The structure and morphology, optical properties, and photoelectrochemical properties of the prepared photocatalyst were characterized via a series of characterization techniques. The average fluorescence lifetime of the composite photocatalyst was extended from 6.67 ns to 7.30 ns in comparison with that of g-C3N4, which indicated that the composite photocatalyst enhanced the absorption of visible light and also inhibited the recombination of electron–hole pairs. The mass ratio of MoS2 that corresponded to O-C3N4/MoS2-5 enabled the highest removal rate under simulated sunlight irradiation, which was almost twice that achieved using pure g-C3N4. Relative species scavenging experiments revealed that ·O2− was the main species during the process of photodegradation. Besides, a toxicity test indicated that glyphosate became less toxic or non-toxic after photodegradation. This study provided an effective, feasible and stable photocatalyst driven by simulated sunlight irradiation for the highly enhanced photodegradation of glyphosate.
研究领域:
环境科学
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材料科学
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ANALYST
·
2020
·Vol. 146
·期 3
·956-963
·
IF 3.978
Jun Ma,Gege Feng,Ying Ying,Yong Shao,Yongxin She,Lufei Zheng,A. M. Abd EI-Aty,Jing Wang
Herein, an indirect SERS sensing assay was developed for the determination of glyphosate (Gly) in tap water. The mechanism of detection was based on relieving the inhibitory effect of L-cysteine (L-cys) on a Au–Pt nanozyme by combining Gly with L-cys through divalent copper ions (Cu2+). In this method, a novel nano-chain-like Au–Ag composite with good repeatability was successfully fabricated to detect SERS signals of oxTMB without disturbing TMB Raman signals. Under optimal conditions, the SERS signal intensity of oxTMB (at 1605 cm−1) was proportional to the concentration of Gly. The results showed a good linear response over the concentration ranges of 10 μg L−1 to 1000 mg L−1. The limit of detection and limit of quantitation of Gly were found to be 5 μg L−1 and 10 μg L−1, respectively. In addition, good anti-interference ability against interfering cations and structural analogues deserves to be mentioned. This SERS assay can be used for detection of Gly in tap water that can meet the needs of practical detection.
研究领域:
分析化学
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环境科学
·
纳米技术
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Qianji Li,Zejia Hao,Chenchen Zhang,Shanhong Ni,Ping Jiang,Pengfei Fan,Lei Li
In this article, a dual-signal sensor for the fluorescence and colorimetric detection of glyphosate (Gly) is developed based on DNAzyme-mediated click chemistry and DNAzyme-regulated CeO2 peroxidase-like activity. DNAzyme can bind to Cu+, triggering a click chemistry reaction between 3-Azido-7-hydroxycoumarin (AHC) and 3-ethyn-1-ol (BOL), thus generating a strong fluorescence signal at 475 nm. Due to the strong coordination between Gly and Cu2+, the amount of reduced Cu+ decreases, resulting in a weakening of the fluorescence. In addition, Gly can inhibit the catalytic site of CeO2 enzyme activity, while DNAzyme, which does not participate in the click chemistry reaction, can be adsorbed by CeO2, further inhibiting the enzyme activity and reducing the oxidation color change of 3,3′,5,5′-tetramethylbenzidine (TMB). The fluorescence detection limit of this dual-mode sensing platform is 0.15 μg/mL, and the colorimetric detection limit is 0.19 μg/mL. This method has been successfully applied to the detection of Gly in tap water and soybeans, which has a promising application in pesticide residue detection.
研究领域:
分析化学
·
生物传感器
·
环境科学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Pingli Dai,Zhenxiong Yan,Shilong Ma,Yang Yang,Qiang Wang,Chunsheng Hou,Yanyan Wu,Yongjun Liu,Qingyun Diao
Effects of glyphosate on survival, developmental rate, larval weight, and midgut bacterial diversity of Apis mellifera were tested in the laboratory. Larvae were reared in vitro and fed diet containing glyphosate 0.8, 4, and 20 mg/L. The dependent variables were compared with negative control and positive control (dimethoate 45 mg/L). Brood survival decreased in 4 or 20 mg/L glyphosate treatments but not in 0.8 mg/L, and larval weight decreased in 0.8 or 4 mg/L glyphosate treatments. Exposure to three concentrations did not affect the developmental rate. Furthermore, the intestinal bacterial communities were determined using high-throughput sequencing targeting the V3–V4 regions of the 16S rDNA. All core honey bee intestinal bacterial phyla such as Proteobacteria (30.86%), Firmicutes (13.82%), and Actinobacteria (11.88%) were detected, and significant changes were found in the species diversity and richness in 20 mg/L glyphosate group. Our results suggest that high concentrations of glyphosate are deleterious to immature bees.
研究领域:
生态毒理学
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微生物学
·
昆虫学
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2021
·
IF 6.291
Zhi Zhou,Xingzhen Ni,Zhongjie Wu,Jia Tang
The amino acid metabolism-related herbicides glufosinate and glyphosate are used worldwide and have flowed into the oceans, threatening the marine organisms. In the present study, physiological activities and transcriptomic profiles of the scleractinian coral Pocillopora damicornis and symbiotic Symbiodiniaceae were determined during a 48 h-exposure to the two herbicides with the final concentration of 10 μmol L −1 . Coral samples were collected at 0, 12, 24, and 48 h after exposure to determine symbiont density, chlorophyll content, as well as activities of superoxide dismutase (SOD), catalase (CAT), nitric oxide synthetase (NOS) and phenoloxidase (PO), and the caspase-3 levels, and the samples collected at 24 h were employed in the transcriptomic analysis. Specifically, the symbiont densities did not change significantly in response to the two herbicides, while the chlorophyll content increased significantly at 24 h post glufosinate exposure. SOD and CAT activities in the coral host increased significantly at 12 h after glufosinate and glyphosate exposure, while the activity of NOS in symbionts decreased significantly at 48 h after glufosinate exposure. Caspase-3 levels in the coral host declined significantly at 24 h after exposure to the two herbicides. In the transcriptomic analysis, glufosinate triggered the expression of genes related to the response to stimuli and immunoregulation in the coral host, and suppressed the expression of genes related to coral nitrogen-related metabolism, symbiont cell cycle, and response to nutrient levels. Furthermore, glyphosate activated the expression of genes involved in coral calcification and symbiont nutrient export and suppressed the expression of genes involved in coral meiosis and symbiont cell communication. These results suggest that although the coral-Symbiodiniaceae symbiosis is not disrupted, short-term glufosinate and glyphosate exposures alter several essential physiological processes including metabolism, calcification, and meiosis in the coral host, as well as the cell cycle and nutrient export in the symbiont. Summary Glufosinate and glyphosate herbicide exposures can disturb several essential physiological processes, including metabolism, calcification, and meiosis in the coral host as well as the cell cycle and nutrient export in the symbiont, threating the survival of scleractinian corals.
研究领域:
海洋生物学
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生态毒理学
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分子生物学
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珊瑚礁生态学
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MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 3
·1-11
·
IF 6.408
Qiang Yu,Yang Weixia,Zhang Xiaoshuo,Luo Xueli,Tang Wenzhi,Yue Tianli,Li Zhonghong
A nanocomposite was prepared by loading UiO-67 nanoparticles onto porous carbon materials derived from Ce-MOF (Ce-PC) for fluorescence detection of glyphosate. The probe (UiO-67/Ce-PC) exhibits fluorescence emission at 414 nm as the response signal under excitation at 310 nm. The fluorescence enhancement mode of UiO-67 reduces the background interference, and the introduction of Ce-PC provide hierarchical nanostructure and large specific surface area that can increase the contact availability and improve the pre-enrichment effect, ensuring UiO-67/Ce-PC with superior sensitivity. The abundant metal hydroxyl group (M–O-H) of UiO-67/Ce-PC could recognize phosphoryl groups (-PO 3 H 2 ) of glyphosate through ligand exchange, which synergizes with H-bonding interaction and electrostatic attraction to exhibit specificity toward glyphosate. The competitive coordination effects weaken the ligand-to-metal charge transfer (LMCT) and consequently induce the fluorescence recovery. The calibration plot of the fluorescence enhancement response of UiO-67/Ce-PC towards glyphosate was recorded in the range 0.02–30 μg mL −1 with a low limit of detection (LOD) of 0.0062 μg mL −1 , which is superior to the pure UiO-67. In addition, the sensor exhibited high selectivity and satisfactory accuracy and precision with recoveries of 92.1–105.6% and RSDs below 3.4%. This work not only presents a feasible sensor for sensitive and selective determination of glyphosate from cereal samples, but also provides a promising strategy for the design of MOF-based nanocomposites to achieve trace detection of various pollutants. Graphical abstract
研究领域:
分析化学
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环境科学
·
材料科学
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高影响因子
危险品化学品经营许可证（带存储）