Food Bioscience
·
2024
·
IF 4.8
Jing Guo,Xiaojie Yu,Cunshan Zhou,Bo Wang,Lei Zhang,Phyllis Otu,Li Chen,Yunwei Niu,Deyang Yao,Chenhui Hua,Haile Ma
Gradient dilution feeding substrate (GDFS) is regarded as an effective method for the production of active peptides, as it compensates for substrate limitations and minimizes substrate contamination of the ultrafiltration membrane. This study further investigated the impact of ultrasound-assisted gradient dilution feeding substrate (UA-GDFS) on the peptide yield and the intensity of umami. The chicken breast was used as ingredients. The umami compounds in the hydrolysate were determined using an automatic amino acid analyzer and HPLC. The hydrolysate's umami intensity was determined by electronic tongue in all modes. These findings indicated that UA-GDFS enhanced the umami intensity and peptide yield. Hydrolysate from UA-GDFS with molecular weight below 1 kDa was analyzed by nano-scale liquid chromatography-tandem mass spectrometry (Nano-HPLC-MS/MS). Electronic tongue analysis showed that GPSGPVG and VDGPSGK had umami taste and could enhance the umami taste of monosodium glutamate (MSG) solution. Asn68 and His145 were important sites on T1R3 for umami peptide binding.
研究领域:
食品科学
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化学工程
·
分析化学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Zheng Zhou,Yu-Ying Zhang,Jing-Xuan Gao,Li-Xin Ma,Xu-Hui Huang,Jie Zheng,Xiu-Ping Dong,Lei Qin
This study investigated the changes in free amino acids (FAAs), 5′-nucleotides, and lipid compositions of Manila clams ( Ruditapes philippinarum ) collected from December 2017 to November 2018. High-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry was used to quantify twenty-one FAAs and six 5ʹ-nucleotides. Lipid profiles were explored by ultra-performance liquid chromatography coupled with a Q-Exactive Orbitrap mass spectrometer with an untargeted lipidomic approach. Results showed significant monthly changes of FAAs, 5′-nucleotides, and lipids profiles. Alanine , glutamic acid , glycine, and aspartic acid , which contributed to the sweet and umami flavor, were the most abundant FAAs in Manila clams. Totally 90 glycerides, 76 glycerophospholipids , 21 lyso-glycerophospholipids, and 52 fatty acids were identified in Manila clams. Among these lipids, most of them possessed a high-level of unsaturation degree. The unsaturation degree of lipids in February, March, and April was the highest, showing the best nutritional value of Manila clams.
研究领域:
海洋生物学
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代谢组学
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食品科学
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营养学
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高影响因子
RSC Advances
·
2015
·Vol. 5
·期 103
·84856-84864
·
IF 3.84
Qianchi Ma,Lei Liu,Wei Cui,Ruifeng Li,Tingting Song,Zhaojie Cui
Model aqueous solutions of perfluorooctanoic acid (PFOA, 100 mg L−1) were electro-oxidized in a homemade container. The electrocatalytic behavior and anodic performance of Ti/SnO2–Sb/Yb–PbO2, Ti/SnO2–Sb–PbO2 and Ti/SnO2–Sb–Yb anodes in sodium electrolytes were compared. The SnO2–Sb/Yb–PbO2 anode demonstrated better electrocatalytic performance compared with the SnO2–Sb–PbO2 and SnO2–Sb–Yb electrodes in terms of both degradation and defluorination. Then a systematic experimental study was designed as follows to analyze the influencing factors: initial concentration of PFOA (10 mg L−1 to 200 mg L−1), current density (1 mA cm−2 to 40 mA cm−2), initial pH value (3 to 11) and electrode distance (5 mm to 20 mm). After a 150 min electrolysis, the optimum reaction conditions were obtained and the degradation and defluorination ratios reached 95.11 ± 3.9% and 75.7 ± 2.8%, respectively. Under the optimum conditions, the degradation of PFOA followed pseudo-first-order kinetics (0.0193 min−1) and the degradation half-life was 35.9 min. The produced F− was measured using a fluoride ion selective electrode, whereas the intermediate PFCAs with short-chain lengths were measured using HPLC-MS. A detailed degradation pathway was proposed in this study by analyzing the intermediates and the recovery of fluoride.
研究领域:
环境科学
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化学工程
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分析化学
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Min Zhang,Muhammad Arif,Yunyuan Dong,Xiaobin Chen,Xiaoheng Liu
In photocatalysis as a “green chemistry” technology, the development of efficient strategies to fabricate high-performance micro-nano photocatalysts with robust solar light utilization to deal with persistent pharmaceutical products in the wastewater system is crucially important. In order to improve the photoelectric activity, interfacial charge transfer, and reduce the recombination rate of photo-generated electron-hole pair, a Z-scheme TiO 2−x @ZnIn 2 S 4 heterojunction was prepared by hydrothermal method . The morphological structure and optical properties of the prepared catalysts were extensively characterized. Experimental results show that the rich of oxygen vacancies in TiO 2−x benefits the formation of local defect energy levels at the bottom of the CB , which can be advantageous to broaden the light absorption range. Likewise, the TiO 2−x @ZnIn 2 S 4 showed 12.4-fold and 7.70-fold higher photocatalytic performance pristine TiO 2−x and ZnIn 2 S 4 towards the degradation of tetracycline hydrochloride (TCH) and nitrofurantoin (NFT) in real water matrix and solution water. Furthermore, the photo-decomposition pathway of NFT was proposed by HPLC-MS, and the Z-scheme electron transfer mechanism in TiO 2−x @ZnIn 2 S 4 heterojunction was proposed and discussed in detail. This work emphasizes a facile method for designing high-efficiency oxygen vacancy mediated titanium-based photocatalysts with excellent photoactivity in environmental remediation.
研究领域:
环境科学
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材料科学
·
化学
·
光催化
·
水处理
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Geng Li,Zhuoyu Cai,Kun Su,Yuxin Zhao,Ying Zhu,Jiangang Han,Yuwei Pan,Weinan Xing,Guangyu Wu
In recent years, advanced oxidation technologies employ peroxynitrite activation have exhibited exceptional effectiveness in eliminating various emerging contaminants. In this study, different calcination temperatures were used to derive Co 3 O 4 nanospheres with varying surface morphologies . This method effectively addresses the issue of high cost associated with high-performance catalyst synthesis or poor catalytic performance of low-cost catalysts. The active species like O 2 •− , •OH, SO 4 •− , 1 O 2 played significant role in TC degradation and 1 O 2 was the dominant reactive oxygen species (ROS). The study also proposed possible degradation pathways based on the 15 intermediates identified through HPLC-MS. Furthermore, the developmental toxicity of the identified intermediates was evaluated using a quantitative conformational relationship prediction method. This paper delves into the reaction mechanism of Co 3 O 4 nanospheres in the activation of PMS and examines the impact of surface morphology on their catalytic performance, providing a new approach to developing a persulfate catalyst that is cost-effective and has high catalytic performance, with a simple synthesis step.
研究领域:
环境科学
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化学工程
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材料科学
·
高级氧化技术
·
催化
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高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2022
·
IF 3.318
Guiya Yang,Pai Liu,Huan Shi,Weixu Fan,Xie Feng,Jiabao Chen,Songsong Jing,Lei Wang,Yuguang Zheng,Dan Zhang,Long Guo
The rhizome of Dioscorea nipponica Makino (RDN) is a widely used herbal medicine, which has significant anti-inflammatory activities on various inflammatory diseases. However, the bioactive compositions responsible for the anti-inflammatory activity of RDN are still unknown. This study aimed to identify the anti-inflammatory bioactive compounds in RDN using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q/TOF-MS), quantitative analysis of multiple components by single marker (QAMS) and chemometric methods. Firstly, an HPLC-Q/TOF-MS method was employed for identification of bioactive steroidal saponins in RND, and a total of twelve steroid saponins were identified. Then, QAMS method was employed to determine the contents of seven bioactive steroidal saponins, including protodioscin, protogracillin, methyl protodioscin, pseudoprotodioscin, pseudoprogracillin, dioscin and gracillin in RND samples using dioscin as the reference analyte. The anti-inflammatory effects of RDN samples were then evaluated by inhibition of NO production in LPS-induced RAW264.7 cells. Furthermore, chemometric methods, including Pearson correlation analysis and partial least squares regression (PLSR) were employed to investigate the correlations between chemical components and anti-inflammatory activities, and explore the potential anti-inflammatory bioactive compounds of RDN. The results indicated that protodioscin, dioscin and gracillin were selected as the major anti-inflammatory compounds in RND. The further verification experiments showed that protodioscin, dioscin and gracillin exhibited great inhibition on NO production with IC 50 values (the half maximal inhibitory concentration) of 0.712 μM, 0.469 μM and 0.815 μM, respectively. They also significantly reduced the levels of TNF-α, IL-1β, and IL-6 in LPS-induced RAW264.7 cells. The present study provided evidences for the anti-inflammatory activity of RND and identification of the anti-inflammatory components in RDN.
研究领域:
生药学
·
药物分析
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药理学
·
分析化学
·
生物化学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ruidong Zhou,Cong Kong,Yupeng Wen,Guangxin Yang,Wendi Huo,Chaoying Zhang,Huiwu Sun,Huan Liu,Dongmei Huang,Jincheng Li
A 15-channel pressure filtration purification method was presented for high throughput sample preparation of aquatic products. A cost-effective device was constructed and melamine sponge was selected as the cleanup sorbent. Upon interfacing with HPLC-MS/MS, the analytical procedure demonstrated its suitability for quantifying 160 pesticides and veterinary drug residues in aquatic products such as fish, shrimp, and crab. The method achieved sample recoveries ranging from 61.3 to 124.9 %. The detection limits were established between 0.5 and 1.0 μg/kg, while the quantitation limits were confirmed to be within the range of 1.0–2.0 μg/kg. The method was applied to quantify the pesticide and veterinary drug residues in mostly consumed aquatic products from five coastal provinces in China. The results showed significant differences between different aquatic products in the concentrations of pesticide and veterinary drug residues, implying the necessity of supervision for the accurate determination of pesticides and veterinary drugs.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
TUBERCULOSIS
·
2017
·
IF 2.873
Shouhong Gao,Zhipeng Wang,Xinfang Xie,Chunhua You,Yang Yang,Yanhai Xi,Wansheng Chen
First-line anti-tuberculosis drugs are playing vital roles for curbing rapid spread of tuberculosis. Multidrug therapies are commonly applied in clinical to achieve better treatment outcomes. However, drug resistance and adverse reactions come along with this therapies and therapeutic drug monitoring is a feasible way to precaution them. For this reasons, a simple and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) and single protein precipitation was developed and validated for simultaneously quantifying of pyrazinamide, isoniazid, ethambutol , streptomycin and rifampicin in human plasma. Optimized chromatographic separation was achieved on a ZORBAX SB-C 18 column with heptafluorobutyric acid, an ion-pair reagent, in the mobile phase at a flow rate of 0.3 mL/min. The mass detection was achieved using electrospray ionization in the positive ion mode with a multiple reaction monitoring mode. The lower limit of quantification (LLOQ) and dynamic range of pyrazinamide, isoniazid, ethambutol, streptomycin and rifampicin were 200–4000 ng/mL, 80–2000 ng/mL, 0.2–1000 ng/mL, 2000–200000 ng/mL and 200–4000 ng/mL, respectively. The Inter-day and intra-day accuracy and precision were within ±15.0% and less than 15%. The method had been successfully applied to simultaneous determination of four first-line Anti-tuberculosis drugs in plasma from tuberculosis patients.
研究领域:
临床医学
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药理学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2022
·
IF 4.132
Lei Zhu,Li Liu,Le Du,Jingmei Hao,Jianhua Ma,Jun Zhu,Wenjing Gong
A rapid, accurate, and selective analytical method to simultaneously quantify 13 anticoagulant rodenticides in animal biological samples was developed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) coupled with electrospray ionization (ESI) in negative mode. Samples were extracted and purified based on a modified QuEChERS (quick, easy, cheap, effective, rugged, safe) sample preparation technique. The sample pH and the type of extraction solvent and cleanup sorbent used to estimate the procedure’s effectiveness were optimized. To improve the matrix effects and obtain acceptable recoveries for 13 rodenticides, 0.1 mL/g biological sample and 1 mL acetonitrile (or acetonitrile: EtOAc = 1:1/(v:v)) extraction followed by Florisil/HC-C18/anhydrous Na2SO4 (NaCl) cleanup under alkaline conditions was fully validated and shown to be selective, precise, accurate, and linear in the range from 1 to 100 ng/mL (g). The mean recoveries were between 52.78 and 110.69%, while the limits of detection and quantification ranged from 0.05 to 0.5 and 0.1–1 ng/mL (μg/kg), respectively. Ideal soft matrix effects (≤20%) were observed for the vast majority of rodenticides (>95%) showing either suppression or enhancement. This method meets international criteria and is capable of simultaneously identifying and quantifying anticoagulant rodenticides in animal blood and tissues and can be suitable for the detection of poisoning cases in the field of forensic or public health.
研究领域:
分析化学
·
毒理学
·
法医学
·
公共卫生
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DOI
PubMed
高影响因子
MOLECULES
·
2026
·Vol. 31
·期 11
·1780
·
IF 5.1
Xiaohui Cai,Jun Men,Qingwu Yang,Yili Hu,Zhixian Qiao
A derivatisation-free HPLC–MS/MS method was developed and validated for the simultaneous quantification of selenium- and sulphur-containing amino acids in soybean leaves, and applied to a 3 × 3 factorial hydroponic experiment probing selenium–sulphur metabolic interactions. The method resolves five biologically informative analytes (Cys2, SeCys2, MeSeCys, Met, SeMet) within 1.5 min through multiple reaction monitoring (MRM). Ultrasound-assisted extraction (UAE) of the free fraction was jointly optimised for both analyte classes by the response-surface methodology; enzymatic hydrolysis of the extraction residue recovered the protein-bound fraction on the same platform. Limits of detection ranged from 0.036 to 0.556 µg L−1, intra-day relative standard deviations were below 5%, and spike recoveries fell between 92.3 and 117.4%. Free SeAA and SAA pools were negatively correlated across the nine treatments (R2= 0.83), consistent with competitive Se–S assimilation, whereas bound pools were positively correlated (R2= 0.89), reflecting proportional protein-level incorporation. A regime of 1–5 mM of sulphate with 20 µM of selenite yielded the highest bound organo-Se with near-normal growth, providing leaf-level evidence that may inform future seed-focused studies aimed at Se-enriched soy-protein ingredient development.
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DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2026
·
IF 6.1
Shengnan Wang,Shijia Lu,Guofei Li,Yingjie Qi
Purpose Hepatocellular carcinoma (HCC) is a highly lethal malignancy with poor prognosis and limited clinical treatments. CD8⁺ T cell-based immunotherapy offers promising therapeutic potential against HCC. We fabricated MMP-9/pH dual-sensitive liposomes co-encapsulating enzalutamide, GB-1107 and BMS-1166 for HCC treatment. Reliable detection approaches are lacking to assess in vivo liposomal behavior. This study established an HPLC-MS/MS method for simultaneous determination of the three drugs in rat plasma, and applied it to characterize their pharmacokinetic profiles.Methods The pH/MMP-9 dual-sensitive liposomes co-loaded with BMS-1166, GB-1107 and enzalutamide were prepared via the thin-film hydration method, and the resultant formulations exhibited satisfactory pharmaceutical properties meeting the predefined quality criteria. For chromatographic separation, a poroshell 120 EC-C18 column (50 × 4.6 mm, 2.7 μm) was adopted, combined with linear gradient elution of 0.1% formic acid in water and acetonitrile. Full validation of the established method was performed strictly following the guidelines for bioanalytical method validation as stated in the Chinese Pharmacopoeia.Results BMS-1166, GB-1107, and enzalutamide exhibited good linearity over the concentration range of 1.0–1000.0 ng/mL. The accuracy and precision of BMS-1166, GB-1107, and enzalutamide all fall below 11.1%. In addition, the recoveries and matrix effect for these analytes ranged from 88.6%-99.7%. Pharmacokinetic results demonstrated that the prepared liposomes possessed favorable in vivo long-circulating properties, which prolonged the half-lives of BMS-1166 and GB-1107 by 25-fold and 13-fold, respectively, and enhanced their bioavailability by 35-fold and 30-fold, respectively. In contrast, no statistically significant effect was observed on enzalutamide.Conclusion The established method was validated and met the predefined quality criteria, thereby being suitable for investigating the in vivo pharmacokinetic profiles of BMS-1166, GB-1107, and enzalutamide.
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-8
·
IF 5.479
Jiang Yanxiao,Ma Pinyi,Piao Huilan,Qin Zucheng,Tao Shuo,Sun Ying,Wang Xinghua,Song Daqian
Cellulose paper was coated with the metal-organic framework MIL-101(Cr) in a chitosan matrix and utilized for thin-film microextraction (TFME). The coated paper possesses excellent extraction efficiency for the triazine herbicides atraton, desmetryn, secbumeton, prometon, ametryn, dipropetryn, and dimethametryn. High-performance liquid chromatography-tandem mass spectrometry was applied to quantify target analytes. The effects of mass ratio of MIL-101(Cr) to chitosan, sample pH value, time of adsorption and desorption, and type and volume of desorption solvent on extraction efficiency were optimized. Under the optimal conditions, the method has limits of detection between 1.5 and 22 ng·L −1 . The recoveries of triazines from spiked tap water, drinking water, lake water and river water range from 77.0 to 125.3%, with relative standard deviations of <17.4%.
Cellulose paper was modified with metal-organic framework MIL-101(Cr)/chitosan by using a simple, efficient and environmentally friendly approach. The modified cellulose paper was then used as a novel extraction phase in thin-film microextraction (TFME).
研究领域:
分析化学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）