Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 20
·10766-10775
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IF 11.9
Nan Zou,Jie Chen,Ting Qiu,Ying Zheng
Direct hydrogenation of CO2 to ethanol is a highly economical carbon reduction strategy. However, the CO2 hydrogenation process traditionally requires high temperatures, typically higher than 300 °C, to activate CO2 molecules, which is unfavorable to the formation of ethanol. In contrast, we applied the novel non-thermal plasma to activate CO2 at ambient conditions and a Cu(I)-MOF catalyst to selectively synthesize ethanol for the selective transformation of CO2 into ethanol. The best catalyst, Cu(I)-HKUST-17.5, achieved 41.2% CO2 conversion and up to 62.9% ethanol selectivity under ambient conditions. The control experiments indicated that the Cu(I) sites are key to realizing C–C coupling and ethanol synthesis. An unreported synergistic catalytic mechanism between non thermal plasma and the Cu(I) and Cu(II) sites on the catalyst is proposed based on the DRIFTS analysis. Overall, our work confirms the feasibility of utilizing non thermal plasma assisted catalysis for the selective conversion of CO2 to ethanol under mild conditions.
研究领域:
化学工程
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环境科学
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产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2016
·
IF 4.219
Lei Tao,Fei Song,Naiyu Xu,Duxin Li,Robert J. Linhardt,Zhenqing Zhang
Hyaluronic acid (HA), a glycosaminoglycan, is a linear polysaccharide with negative charge, composed of a repeating disaccharide unit [→4)-β- d -glucopyranosyluronic acid (1 → 3)-β- d - N -acetyl- d -glucoaminopyranose (1 → ] n ([→4) GlcA (1 → 3) GlcNAc 1 → ] n ). It is widely used in different applications based on its physicochemical properties associated with its molecular weight. Enzymatic digestion by polysaccharides lyases is one of the most important ways to decrease the molecular weight of HA. Thus, it is important to understand the action patterns of lyases acting on HA. In this study, the action patterns of two common lyases, Flavobacterial chondroitinase AC I and Streptomyces hyaluronidase, were investigated by analyzing HA oligosaccharide digestion products. HA oligosaccharides having an odd-number of saccharide residues were observed in the products of both lyases, but their distributions were quite different. Chondroitinase AC acted more efficiently at the β 1–4 glycosidic bond linking GlcNAc and GlcA. Oligosaccharides, having an even number of saccharide residues, and with an unsaturated uronic acid (4-deoxy-α- l - threo -hex-4-enepyranosyluronic acid, △UA) residue at their non-reducing end represent the major product. A minor amount of oligosaccharides having an odd number of saccharide residues resulted from the irregular terminal residues of HA substrate chains. Hyaluronidase showed a more complicated product mixture. Its minimum recognition and digestion domain is HA heptasaccharide and it could cleave both β 1–4 and β 1–3 glycosidic linkages. The HA oligosaccharides, generated with a 2-acetamido-2,3-di-deoxy-β- d - erythro -hex-2-enopyranose (△HexNAc) at the non-reducing end, are believed to be unstable and undergo breakdown immediately after their generation, and the oligosaccharides with △UA residue at the non-reducing end are formed. Thus, oligosaccharides having both an even and odd-number saccharide residues with a △UA residue at their non-reducing ends, represent the major products of hyaluronidase acting on HA.
研究领域:
生物化学
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分子生物学
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DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2022
·Vol. 6
·期 1
·12-19
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IF 0
Wang Xin-Yan,Li Yuanfang,Lv Shuzhen,Bi Sai
Herein, a dual-signal electrochemical biosensor has been developed by self-assembly of pH-activatable i-motif probes on magnetic microparticles (MMPs) coupled with DNA walker for signal amplification. In this study, the cytosine (C)-rich single-stranded DNAs are hybridized with DNA initiators to obtain the long-nicked duplexes with repeated units, which are further captured on MMPs to form the magnetic i-motif containers. The resulting duplexes contain abundant G–C base pairs, thus providing extensive binding sites for doxorubicin (DOX). At acidic pH, the C-rich sequences are folded into i-motif structure, resulting in the release of DOX and walker initiators. In this case, the liberated DOX is adsorbed on graphene quantum dots-modified glassy carbon electrode via π–π interaction, while the walker initiators as a moving part can catalyze the hybridization between MB-modified fueler DNA and tracker DNA on electrode, contributing to the generation of dual electrochemical signals induced by MB and DOX. Importantly, the magnetic separation can effectively reduce the background, achieving sensitive biosensing of pH ranging from 4.0 to 7.4 with excellent stability. Moreover, the proposed dual-signal electrochemical biosensor has been successfully applied for accurate monitoring of pH in human serum, which holds great potential in pH-dependent bioassays, especially in ultra-micro analysis for clinical applications.
研究领域:
生物化学
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分析化学
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医学诊断
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DOI
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-13
·
IF 3.9
Xia Meng,Cao Shisheng,Liu Zeqi,Zhao Jiakang,Yuan Yibo,Ni Jianhui,Guo Zhengjian,Wang Weihan,Yin Huijuan,Zou Zhaohui
Persistent or refractory apical periodontitis is primarily caused by microbial retention, as conventional root canal treatment often fails to eliminate infections completely, and systemic antibiotic therapy is insufficient to achieve effective concentrations for eradicating bacterial biofilms within root canals. This highlights the urgent need for novel therapeutics offering safe and effective antimicrobial strategies. Antimicrobial photodynamic therapy (aPDT) is a promising approach for root canal disinfection. However, commonly used photosensitizers such as Ce6 suffer from poor water solubility and strong aggregation tendencies, resulting in limited penetration into infected sites. In this study, we developed a DNase I-Lip@Ce6 nanodelivery system by combining deoxyribonuclease I (DNase I) with liposome-encapsulated Ce6. The liposomal carrier facilitated efficient delivery of Ce6 into target bacterial cells, while DNase I degraded extracellular DNA in the biofilm matrix, weakening its protective barrier. This synergistically enhanced Ce6 penetration and therapeutic efficacy, leading to the successful eradication of planktonic Enterococcus faecalis and in vitro biofilms. This strategy offers a novel approach for the precision treatment of persistent oral infections and holds strong potential for clinical translation.
研究领域:
口腔医学
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微生物学
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药剂学
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生物材料
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产品关联
DOI
PubMed
高影响因子
MOLECULAR CRYSTALS AND LIQUID CRYSTALS
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2020
·
IF 0.512
Chanchan Xu,Lu Lu,Le Lv,Jiandong Guo,Chaoyun Luo,Dawei Luo,Wei Liu
A new zero-dimensional (0D) silver (I) chloride based coordination complex 0 D-Ag2Cl2(L) (1, L = (oxybis(2,1-phenylene))bis(diphenylphosphine)) has been prepared by mixing the silver(I) salt and a bidentate P-ligand at room temperature in solution. 1exhibits a band gap of 3.5 eV and a decomposition temperature (Td) of 280 °C. Such a high Td for molecular complex is a result of the strong Ag-P bonding and the “chelating effect” of the metal of the bidentate P-ligand. It has been used as an efficient photocatalyst for the degradation of organic dyes in water.
研究领域:
材料化学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF ORGANOMETALLIC CHEMISTRY
·
2023
·
IF 2.3
Yi-Fan Tan,Guang-Min Liang,Kun Zhou,Jiu-Yu Ji,Li-Ping Zhao
A new three-dimensional (3D) supramolecular compound, [Ag(dppe)(CF 3 COO)] n ( 1 ), was prepared by using Ag(I) as the silver source and dppe as the organic ligand through the self-assembly approach. The bridging dppe ligand makes compound 1 appear as a one-dimensional (1D) wavy chain consolidated by the intramolecular hydrogen bonds. Two adjacent 1D chains are expanded to a two-dimensional (2D) layer passing through the intermolecular C H···π interaction between the dppe ligands, and the 2D layers further weave into a 3D supramolecular structure via another intermolecular C H···π interactions between the dppe ligands. The precise structure of 1 was determined by single crystal X-ray diffractometer (SCXRD), and it was well-characterized by Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) spectroscopy, powder X-ray diffraction (PXRD), thermogravimetric analysis (TG) and differential thermogravimetry analysis (DTG), and UV–vis diffuse reflectance spectrum. In addition, the photocurrent response, electrochemistry characteristic and photodegradation of 1 were investigated. The degradation efficiency (82 %) of 1 as catalyst to degrade methylene blue (MB) was significantly higher than that without catalyst.
研究领域:
化学
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材料科学
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产品关联
DOI
高影响因子
Journal of the American Chemical Society
·
2009
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IF 8.091
Yinghua Peng,Xiaohui Wang,Yi Xiao,Lingyan Feng,Chao Zhao,Jinsong Ren,Xiaogang Qu
The increasing worldwide demand for carbon nanotubes (CNTs) and increasing concern regarding how to safely develop and use CNTs are requiring a low-cost, simple, and highly sensitive CNT detection assay for toxicological evaluation and environmental monitoring. However, this goal is still far from being achieved. All the current CNT detection techniques are not applicable for automation and field analysis because they are dependent on highly expensive special instruments and complicated sample preparation. On the basis of the capability of single-walled carbon nanotubes (SWNTs) to specifically induce human telomeric i-motif formation, we design an electrochemical DNA (E-DNA) sensor that can distinguish single- and multiwalled carbon nanotubes both in buffer and in cell extracts. The E-DNA sensor can selectively detect SWNTs with a direct detection limit of 0.2 ppm and has been demonstrated in cancer cell extracts. To the best of our knowledge, this is the first demonstration of a biosensing technique that can distinguish different types of nanotubes. Our work will provide new insights into how to design a biosensor for detection of carbon nanotubes.
研究领域:
生物传感
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环境监测
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毒理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
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IF 4.784
Xingyu Lu,Qian Zhong,Jian Liu,Fulin Yang,Chenghui Lu,Huan Xiong,Sha Li,Yibo Zhu,Lingtian Wu
Chondroitinase ABC I (ChSase ABC I) is a key enzyme of chondroitin sulfate (CS) degradation and widely used for CS detection in the medicine filed. However, the recombinant ChSase ABC I was weakly expressed in Escherichia coli because the forms of it were mostly inclusion bodies. In this study, a signal peptide ( pel B) was used for the soluble form expression of ChSase ABC I in E. coli . Then the culture condition for ChSase ABC I expression was optimized through response surface methodology. Results revealed that the expression level of ChSase ABC I in a 7.5 L fermentor (29.03 mL −1 ) was approximately 1.65-fold higher than that of the shake flask level (17.55 mL −1 ). The enzymatic properties and kinetic constants of recombinant ChSase ABC I were also studied. Recombinant ChSase ABC I was also used to detect the specific disaccharides content of CS from different sources. This study not only eliminates the problem of the enzyme expressed as an inclusion body, but also solves the current problem of expensive ChSase ABC. In a word, it would be an ideal strategy for ChSase ABC high-efficiency expression and a great method to detect specific disaccharides of CS in biomedical field.
研究领域:
生物技术
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医学
·
酶学
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DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2019
·Vol. 30
·期 16
·14977-14986
·
IF 2.195
Tang Hongbo,Li Qiuyue,Zhou Jian
The effect of low I/Ag ratios on the performance of silver thick films containing micro-sized silver particles treated with surface iodination has been investigated. The film with I/Ag ratio of 1:100 exhibited high electrical conductivity and densification, while that of 2:100 presented better adhesion. The conductivity and densification of the film reached the highest at 550 °C. Optimal conductivity of the film with I/Ag ratio of 1:100 and 2:100 was 11.45 and 15.85 mΩ/sq., respectively. In addition, the film adhesion was enhanced by heating. Different trends observed in electrical conductivity and adhesion as functions of temperature is primarily attributed to shrinkage of modified particles. This work suggested that the I/Ag ratio plays a vital effect on the performance of the silver thick film.
研究领域:
材料科学
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电化学
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表面化学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Xiaomeng Yang,Fei Geng,Zhanghan Shi,Dan Wu,Armin Rezayan,Jingpeng Zhao,Renfeng Nie,Jianshe Wang,Chunbao Xu,Yongsheng Zhang
Reductive etherification of carbonyl compounds using metal–acid bifunctional catalysts is an important strategy for the synthesis of ethers. Conventional metal–acid bifunctional catalysts suffer from poor synergistic effects due to the spatially separated distribution of hydrogenation sites on metal nanoparticles and acid sites on supports. In this study, bifunctional Ni-I catalysts with intimate metal–acid bifunctional sites were prepared through the surface modification of Ni nanoparticles with organic iodide under H 2 atmosphere. Hydrogenolysis of C-I bonds over Ni nanoparticles promotes the selective deposition of I atoms on the Ni surface. Electronegative I atoms withdraw electrons from Ni nanoparticles, which enables the heterolytic dissociation of H 2 over Ni-I polar sites, leading to the in-situ generation of Ni(H - )-I(H + ) bifunctional sites on the metal surface. The reductive etherification of furfural and ethanol over Ni-I bifunctional catalysts demonstrated high efficiency with an 89.4 % yield to furfuryl ethyl ether (FEE) at mild reaction conditions. Mechanistic studies revealed that both the selective deactivation effect and the in-situ generation of Brønsted acidity contribute to this reaction pathway. While the I atoms selectively deactivate the consecutive Ni sites, suppressing the over-hydrogenation of the furan ring, the in-situ generated Brønsted acid sites catalyze the acetalization. Furthermore, temperature-programmed pyridine FTIR analysis revealed that the Brønsted acidity on the Ni-I catalyst is only evident at high temperatures (e.g., over 100°C) due to the high dissociation energy required for H 2 on non-noble metal surfaces. The developed Ni-I bifunctional catalysts, featuring intimate metal–acid sites and low-cost, holds great promise for the catalytic upgrading of biomass-derived oxygenates into fine chemicals.
研究领域:
催化化学
·
生物质转化
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绿色化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2016
·
IF 7.145
Hanbang Chen,Yunzhu Qian,Yang Xia,Gang Chen,Yun Dai,Na Li,Feimin Zhang,Ning Gu
The topographical features and material composition of scaffolds have a powerful influence on cell behaviors such as proliferation and differentiation. Here, scaffolds consisting of aligned fibers with incorporated bioactive collagen I were tested for their ability to enhance osteogenesis in vitro. Rat adipose-derived mesenchymal stem cells (ADSCs) were seeded on the scaffolds and their morphology, proliferation, and osteogenic differentiation were examined. Aligned scaffolds with collagen I showed the best osteogenic properties. Also, adhesion-related genes showed the higher expression on aligned scaffolds with collagen I. Our findings indicate that fiber alignment combined with incorporation of collagen I increases the capacity of electrospun scaffolds to induce enhanced and directed osteogenesis. Such scaffolds may, therefore, have potential for improving guided oral bone regeneration.
研究领域:
组织工程
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再生医学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
Nature Plants
·
2024
·
IF 15.8
Zhang Aihong,Tian Lin,Zhu Tong,Li Mengyu,Sun Mengwei,Fang Ying,Zhang Yi,Lu Congming
Photosystem I (PSI) is one of two large pigment–protein complexes responsible for converting solar energy into chemical energy in all oxygenic photosynthetic organisms. The PSI supercomplex consists of the PSI core complex and peripheral light-harvesting complex I (LHCI) in eukaryotic photosynthetic organisms. However, how the PSI complex assembles in land plants is unknown. Here we describe PHOTOSYSTEM I BIOGENESIS FACTOR 8 (PBF8), a thylakoid-anchored protein in Arabidopsis thaliana that is required for PSI assembly. PBF8 regulates two key consecutive steps in this process, the building of two assembly intermediates comprising eight or nine subunits, by interacting with PSI core subunits. We identified putative PBF8 orthologues in charophytic algae and land plants but not in Cyanobacteria or Chlorophyta. Our data reveal the major PSI assembly pathway in land plants. Our findings suggest that novel assembly mechanisms evolved during plant terrestrialization to regulate PSI assembly, perhaps as a means to cope with terrestrial environments.
研究领域:
植物生物学
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分子生物学
·
光合作用
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）