ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Haoran Tang,Hao Wang,Dandan Zhao,Mengya Cao,Yanyan Zhu,Yongxin Li
Nanopipettes provide a promising confined space that enables advances in single-molecule analysis, and their unique conical tubular structure is also suitable for single-cell analysis. In this work, functionalized-nanopore-based single-entity electrochemistry (SEE) analysis tools were developed for the label-free monitoring of single-molecule glycoprotein–boronate affinity interaction for the first time, and immunoglobulin G (IgG, one of the important biomarkers for many diseases such as COVID-19 and cancers) was employed as the model glycoprotein. The principle of this method is based on a single glycoprotein molecule passing through 4-mercaptophenylboronic acid (4-MPBA)-modified nanopipettes under a bias voltage and in the meantime interacting with the boronate group from modified 4-MPBA. This translocation and affinity interaction process can generate distinguishable current blockade signals. Based on the statistical analysis of these signals, the equilibrium association constant (κa) of single-molecule glycoprotein–boronate affinity interaction was obtained. The results show that the κa of IgG in the confined nanopore at the single-molecule level is much larger than that measured in the open system at the ensemble level, which is possibly due to the enhanced multivalent synergistic binding in the restricted space. Moreover, the functionalized-nanopore-based SEE analysis tools were further applied for the label-free detection of IgG, and the results indicate that our method has potential application value for the detection of glycoproteins in real samples, which also paves way for the single-cell analysis of glycoproteins.
研究领域:
分析化学
·
生物物理学
·
生物化学
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Electronic Materials
·
2023
·
IF 4.7
Xiaoguang Gao,Lingyu Li,Rong Deng,Wenjuan Zhao,Xiaoliang Zhang,Zhibo Liu
Quantitative detection of disease markers at ultralow concentrations has rigorous requirements on the performance of graphene-based optical sensors (GOSs). In this work, a localized surface plasmon resonance-modulated GOS was developed by uniformly dispersing gold nanorods (AuNRs) on the graphene surface. As an optical sensor based on refractive index sensing, the refractive index resolution and sensitivity of the optical sensor reached 2.34 × 10–9 and 2.14 × 107 mV/RIU, respectively. The sensitivity of the optical sensor toward IgG immunoassay reached 6.5 mV (μg mL–1)−1, a value 7-fold that without the AuNRs. A limit of detection of 1.22 ng/mL toward IgG immunoassay was obtained, a value one-tenth that without the AuNRs. Most critically, it has been demonstrated that the improved performance of the optical sensor was attributed to the enhanced evanescent field on graphene caused by the coupling of the evanescent field and LSRR of AuNRs. The proposed optical sensor in this study has the characteristics of high sensitivity, being label-free, and a simple preparation process. The successful application of the proposed sensor will also greatly promote the development of quantitative detection of disease markers at ultralow concentrations.
研究领域:
光学传感
·
纳米技术
·
生物传感器
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2019
·Vol. 9
·期 1
·1901203
·
IF 6.27
Haiying Ji,Guangxi Wu,Yongyong Li,Kun Wang,Xiaomei Xue,Shasha You,Shengming Wu,Tianbin Ren,Bin He,Xueyin Shi
Elevated low-density lipoprotein cholesterol (LDL-C) increases the risk of atherosclerotic cardiovascular disease. Peptide-based PCSK9 vaccines have shown a promising prospect of reducing LDL-C. In peptide vaccine (pVax) design, the peptide antigens need to conjugate with carrier protein (CP). However, CP incorporation can induce undesirable anti-CP antibodies, which sterically mask peptide epitopes from being recognized by specific B cells and impair subsequent therapeutically antibody production. This epitopic suppression has posed a barrier in clinical translation of conjugate vaccines all along. A model CP (keyhole limpet hemocyanin, KLH) is herein camouflaged with serum albumin (SA) into hybrid nanocarriers (SA@N), with PCSK9 peptide being anchored onto the surface to form nanovaccine (SA@NVax). Such camouflage of KLH via high “self” SA coverage is able to inhibit KLH from extracellular immune recognition and prevent detectable anti-KLH antibody production. Furthermore, the nanovaccine around 70 nm stabilized by intermolecular disulfide network is ideal for internalization and biodegradation by antigen presenting cells as well as better retention in draining lymph nodes and spleen. As expected, the SA@NVax efficiently primes higher anti-PCSK9 IgG antibody titer than PCSK9 pVax.
研究领域:
生物技术
·
免疫学
·
药学
·
心血管疾病
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Lingli Lei,Wenyue Xie,Zhaoyang Chen,Ying Jiang,Yingshuai Liu
A novel colorimetric immunoassay strategy is developed based on metal ion chelation-induced color generation and alkaline phosphatase(ALP)-catalyzed signal amplification. The intense purple-colored Cu(I)-bicinchoninic acid (BCA) complex is utilized as a promising chromogenic reporter for the visual ALP-linked immunoassay. In the presence of target, ALP is introduced to catalyze the cascade conversion of L-ascorbic acid 2-phosphate (AAO) to L-ascorbic acid (AA), which results in Cu 2+ reduction to Cu + and subsequently in situ formation of purple-colored Cu(I)-BCA complex. The complex is water-soluble and exhibits a strong absorbance at 562 nm due to the ligand-to-metal charge-transfer (LMCT). The absorbance value is in turn proportional to the level of target analyte. Based on this concept, an ALP-linked colorimetric immunoassay is established with Cu(II)-BCA mixture as a color developing reagent for rabbit IgG detection, achieving a linear range from 0.1 ng mL −1 to 25 ng mL −1 and a LOD of 0.05 ng mL −1 . Its potentiality for practical application is also investigated by detection of a cancer biomarker, prostate specific antigen (PSA), in spiked human serum. A linear range from 0.5 ng mL −1 to 25 ng mL −1 and a LOD at 0.38 ng mL −1 are achieved, which is much lower than the cut-off value of PSA in human blood. The proposed method holds many advantages including low cost, good color stability, reliability and excellent compatibility with the existing ELISA platform, providing a promising colorimetric immunoassay platform for bio-chemical analysis in a variety of fields.
研究领域:
分析化学
·
生物化学
·
免疫学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Mingjun Shi,Yang Li,Wenqing Wang,Rui Han,Xiliang Luo
Overcoming the influence of interfering substances in the environment and achieving superior sensing performance are significant challenges in biomarker detection within complex matrices. Herein, an integrated electrochemical sensing platform for sensitive detection of biomarkers in complex biofluids was developed based on a newly designed PEGylated multifunctional peptide (PEG-MPEP). The designed PEG-MPEP contains a poly(serine) sequence (−ssssss−) as the antifouling part and recognition peptide sequence (−avwgrwh) specific for the target human immunoglobulin G (IgG). To improve the peptide stability to protease hydrolysis, d-amino acids were adopted to synthesize the whole peptide. Additionally, the PEGylation can further enhance the stability of the peptide, and the PEG itself was also antifouling, ensuring superstrong antifouling capability of the PEG-MPEP. The designed PEG-MPEP-based biosensor possessed a high sensitivity for the detection of IgG in the range of 1.0 pg mL−1 to 1.0 μg mL−1, with a low limit of detection (0.41 pg mL−1), and it was capable of assaying targets accurately in real serum samples. Compared with conventional peptide-modified biosensors, the PEG-MPEP-modified biosensor exhibited superior antifouling and antihydrolysis properties in complex biofluid, showcasing promising potential for practical assay applications.
研究领域:
生物传感
·
分析化学
·
生物化学
·
分子生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DRUG DELIVERY
·
2021
·
IF 6.419
Congcong Li,Chaoxi Chen,Yucai Wei,Min Tan,Shuo Zhai,Juebo Zhao,Lu Wang,Tao Dai
It is urgently needed to develop novel adjuvants for improving the safety and efficacy of vaccines. Metal-organic frameworks (MOFs), with high surface area, play an important role in drug delivery. With perfect biocompatibility and green preparation process, the γ-cyclodextrin metal-organic framework (γ-CD-MOF) fabricated with cyclodextrin and potassium suitable for antigen delivery. In this study, we modified γ-CD-MOF with span-85 to fabricate the SP-γ-CD-MOF as animal vaccine adjuvants. The ovalbumin (OVA) as the model antigen was encapsulated into particles to investigate the immune response. SP-γ-CD-MOF displayed excellent biocompatibility in vitro and in vivo. After immunization, SP-γ-CD-MOF loaded with OVA could induce high antigen-specific IgG titers and cytokine secretion. Meanwhile, SP-γ-CD-MOF also significantly improved the proliferation of spleen cells and activated and matured the bone marrow dendritic cells (BMDCs). The study showed the potential of SP-γ-CD-MOF in vaccine adjuvants and provided a novel idea for the development of vaccine adjuvants.
研究领域:
药学
·
免疫学
·
疫苗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2022
·
IF 6.556
Wen-Shu Zhang,Xiao-Peng Liu,Shuai Yue,Ya-Ning Wang,Yue Wang,Zhang-Run Xu
Research on ion channels and their monoclonal antibodies plays a critical role in drug development and disease diagnosis. The current ion channel researches are often not conducted in the microenvironment for cells survival , which restricts the mechanism study of the links between the cell structure and the ion channel function. In this work, we synthesized gold core-4-mercaptobenzonitrile-sliver shell-goat anti-rabbit immunoglobulin G ( [email protected] @ [email protected] ) nanoparticles as surface-enhanced Raman scattering (SERS) nanoprobes for investigating the human ether-a-go-go related gene (hERG) potassium ion channel in cell membranes. This is the first attempt to study ion channels using SERS. Due to the unique core-molecule-shell structure and the silver shell of nanoprobes, strong and stable SERS signal was obtained. With the help of antibodies, the [email protected] @ [email protected] nanoprobes were captured by hERG antibodies and then bonded with hERG ion channels based on the sandwich immune response. The reporter molecule, 4-MBN, displayed a strong and sharp characteristic peak at 2233 cm - 1 in the Raman silent region. The intensity of this peak denoted the concentration of antibodies and the expression of ion channel proteins. We successfully applied this amplification-free method for in-situ imaging the distribution of the hERG ion channel on the transfected HEK293 cell surface at the single-cell level. This hERG ion channel profiling strategy promises a maneuverable tool for ion channel research.
研究领域:
生物物理学
·
纳米技术
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2022
·
IF 4.319
Xiaomeng Tang,Meng Yang,Yongwei Gu,Liangdi Jiang,Yue Du,Jiyong Liu
Purpose The effective treatment of ulcerative colitis (UC) poses substantial challenges, and the aetiopathogenesis of UC is closely related to infectious, immunological and environmental factors. Currently, there is a considerable need for the development of orally bioavailable dosage forms that enable the effective delivery of therapeutic drugs to local diseased lesions in the gastrointestinal tract.Methods Berberine (BBR) and Atractylodes macrocephala Koidz (AM) volatile oil, derived from the Chinese herbs Coptis chinensis Franch and Atractylodes macrocephala Koidz, have anti-inflammatory and immunomodulatory activities. In this study, we prepared colon-targeted pellets loaded with BBR and stomach-targeted pellets loaded with AM volatile oil for the synergistic treatment of UC. The Box–Behnken design and β-cyclodextrin inclusion technique were used to optimize the enteric coating formula and prepare volatile oil inclusion compounds.Results The two types of pellets were spherical and had satisfactory physical properties. The pharmacokinetic results showed that the AUC and MRT values of the dual-targeted (DPs) pellets were higher than those of the control pellets. In addition, in vivo animal imaging confirmed that the DPs could effectively deliver BBR to the colon. Moreover, compared with sulfasalazine and monotherapy, DPs exerted a more significant anti-inflammatory effect by inhibiting the expression of inflammatory factors including IL-1β, IL-4, IL-6, TNF-α and MPO both in serum and tissues and enhancing immunity by decreasing the production of IgA and IgG.Conclusion The DPs play a synergistic anti-UC effect by exerting systemic and local anti-inflammatory and provide an effective oral targeted preparation for the treatment of UC.
研究领域:
药剂学
·
药理学
·
药物递送系统
·
炎症
·
免疫学
·
中药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nutrients
·
2024
·Vol. 16
·期 4
·519
·
IF 4.8
Xiao Li,Xingru Lu,Ming Liu,Yu Zhang,Yujun Jiang,Xinyan Yang,Chaoxin Man
The aim of this study was to investigate the immunomodulatory effects of A2 β-casein (β-CN) in cyclophosphamide-induced immunosuppressed BALB/c mice. Experiments conducted in vitro revealed that A2 β-CN digestive products have potent immunostimulatory activities. Animal studies demonstrated that A2 β-CN improved the immunological organ index reduction trend caused by cyclophosphamide, reduced the pathological damage to the spleen tissue in immunosuppressed mice, increased the release of IL-17A, IgG, and IgA, and reduced the production of IL-4. By regulating the relative abundance of advantageous bacteria likeOscillospira,Lactobacillus, andBifidobacteriaand harmful bacteria likeCoprococcusandDesulfovibrionaceae, A2 β-CN improved gut microbiota disorders in immunosuppressed mice. Moreover, A2 β-CN promoted the production of short-chain fatty acids and increased the diversity of the gut microbiota. Therefore, ingestion of A2 β-CN is beneficial to the host’s immune system and gut health. These findings provide insights for the future application of A2 β-CN-related dairy products.
研究领域:
免疫学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 19
·9362-9368
·
IF 6.97
Peng-Fei Guo,Xi-Ming Wang,Meng-Meng Wang,Ting Yang,Ming-Li Chen,Jian-Hua Wang
Boron-titanate monolayer nanosheets were prepared through a scalable step by step intercalation approach for anchoring 3-mercaptopropyltriethoxysilane (MPTS) on the surface. MPTS provides clickable sites with 4-vinylphenylboronic acid (VPBA) via a thiol–ene (TE) click reaction to obtain monolayer titanate nanosheets with boronic acid ligands immobilized on the surface. The nanosheets obtained are denoted as VPBA-MPTS-TiNSs, with a lateral dimension of a few dozen nanometers and with a thickness of ca. 3.5 nm. The nanosheets exhibit a superior adsorption capacity of 1669.7 mg g−1 and favorable selectivity for the adsorption of glycoproteins by employing immunoglobulin G (IgG) as the protein model. The adsorbed IgG is thereafter readily collected by using 0.1% (m/v) cetane trimethyl ammonium bromide (CTAB) as the eluent. The practical applications of VPBA-MPTS-TiNSs are further demonstrated by the selective adsorption/purification of IgG from human serum.
研究领域:
材料科学
·
纳米技术
·
生物化学
·
蛋白质纯化
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Zhou Sha,Xiaochun Gao,Yijie Wang,Xiaotian Guan,Sihao Zhang,Jingru Zhao,Chunnan Wang,Shuqing Sun
Magnetic-fluorescent microbeads had been extensively used in various applications, particularly in the multiple detection of biological molecules. Currently, most commercially available magnetic-fluorescent microbeads had simple structures and the fluorescence intensity was inversely proportional to the concentration of magnetic particles, which affected performances of microbeads. In this work, a three-dimensional capillary microfluidic system was constructed to produce magnetic-fluorescent core-shell microbeads. Magnetic nanoparticles were dispersed in the core of the microbeads, while the shell consisted of quantum dots (QDs). Core-shell structures restricted fluorescence quenching caused by magnetic nanoparticles in the core area. Moreover, the number of cores could be adjusted precisely, which provided an additional encoding method besides the traditional fluorescence encoding. Three types of dual-encoded core-shell microbeads which exhibited excellent uniformity and fluorescence characteristics with additional magnetic response properties were produced. The self-assembly of silica nanoparticles on the surface of microbeads made the introduction of carboxyl groups easy. Furthermore, the capability of these microbeads for multiplex immunoassays was demonstrated using three types of IgG, and a calibration curve for the detection of human IgG was established using a sandwich immunoassay. These results indicated the potential application of this scheme in the field of biomedical detection.
研究领域:
生物技术
·
纳米技术
·
分析化学
·
生物材料科学
·
免疫测定技术
✓ 已验证
产品关联
DOI
高影响因子
FREE RADICAL BIOLOGY AND MEDICINE
·
2020
·
IF 6.17
Changfeng Hu,Jida Zhang,Suzhen Hong,Haichang Li,Lu Lu,Guanqun Xie,Wenqing Luo,Yu Du,Zhijun Xie,Xianlin Han,Chengping Wen
There exist close relationships among oxidative stress , dyslipidaemia , inflammation, and autoimmune response in patients with systemic lupus erythematosus (SLE). Dysfunction and/or dysregulation of immunocytes is one of the major characteristics of SLE pathogenesis. Lipids play many important roles in biological processes and cellular functions. We hypothesized that oxidative stress-induced aberrant lipid metabolism and integrity presented in immunocytes is one of the early events in patients , thereby leading to enhanced production of IgG autoantibodies and cytokines. Herein, shotgun lipidomics was employed for quantitative analysis of cellular lipidomes in peripheral blood mononuclear cells (PBMC) both freshly isolated from SLE patients and cultured with treatment . The levels of IgG autoantibodies and cytokines in cell culture media and serum samples from lupus-prone mice treated with a natural, powerful antioxidant isotonix OPC-3 were measured by ELISA kits. IgG antibody deposition in glomeruli of the mice was determined by immunofluorescence analysis. Lipidomics analysis of PBMC from 33 SLE patients and 28 healthy controls revealed aberrant lipid metabolism in PBMC from the patients. The changes included significantly reduced plasmalogens , markedly increased lysophospholipids , altered phosphatidylserines , and accumulated 4-hydroxyalkenals. These alterations of lipids in SLE PBMC could be significantly corrected after cultured with the antioxidant in vitro . Parallel to the IgG antibody deposition in glomeruli, the concentrations of cytokines (i.e., IL-10, IL-6, and TNF-α) and autoantibodies (e.g., IgG antiphospholipid and anti-dsDNA antibodies) in culture medium and serum samples from the mice after treatment with the antioxidant were also significantly reduced compared with those of the SLE group. The results clearly demonstrated that correction of the aberrant lipid metabolism led to inhibition of the autoimmune reactions of PBMC after reduction of the increased oxidative stress. The current study also revealed potential drug treatment of SLE with lesser adverse effects through reducing the aberrant lipid metabolism with an effective antioxidant.
研究领域:
免疫学
·
风湿病学
·
脂质生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）