CHEMBIOCHEM
·
2018
·Vol. 19
·期 10
·1060-1065
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IF 2.774
Xueyu Zhao,Gaochao Lv,Ying Peng,Qingzhu Liu,Xi Li,Shanshan Wang,Ke Li,Ling Qiu,Jianguo Lin
Graphical Active duty : An activatable fluorescent probe consisting of a peptide, Arg-Val-Arg-Arg (RVRR), and an aminoluciferin fluorophore is designed and prepared for the responsive and activatable detection of furin activity in living cells. This probe is shown to have “off–on” florescence properties, and in the presence of furin in vitro, its fluorescence intensity is increased by a factor of ≈3.58. Furin, a protein convertase, plays a crucial role in the progression of tumors. In this work, a new fluorescent probe consisting of a peptide, Arg-Val-Arg-Arg (RVRR), and an aminoluciferin fluorophore was designed and prepared for the responsive and activatable detection of furin activity in vitro and in living cells. We demonstrated that this probe could be responsive toward furin with an “off–on” florescence signal and generated an approximately 3.58-fold enhancement in the fluorescence intensity in vitro. Fluorescence imaging in MDA-MB-468 and LoVo cells showed that the probe could be cleaved by overexpressed furin with fluorescence turn-on in MDA-MB-468 cells, and this probe was also found to be capable of discriminating between furin-overexpressing and furin-deficient cell lines. Our research indicates that this probe has great potential for the detection of furin activity in living cells.
研究领域:
生物化学
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分子生物学
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癌症研究
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细胞生物学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Feika Bian,Lingyu Sun,Lijun Cai,Yu Wang,Yuanjin Zhao,Shuqi Wang,Mengtao Zhou
As a new class of two-dimensional (2D) materials, molybdenum disulfide (MoS 2 ) has huge potential in biomedical area; while its applications in multiplex bioassays are still a challenge. Here, we present novel MoS 2 -integrated silica colloidal crystal barcode (SCCB) for multiplex microRNA (miRNA) screening. MoS 2 was adsorbed on SCCBs by electrostatic interaction , and quantum dots (QDs) decorated hairpin probes were coupled on MoS 2 by covalent linkage. As the MoS 2 could quench the QDs of the hairpin probes, they together formed a molecular beacon (MB) structure before the detection. When used in assays, target miRNA could form a double strand with the probe and made QDs keep away from MoS 2 sheets to recovery their fluorescence. Because the released QDs were positively correlated with the concentration of the hybridized nucleic acid , the target miRNAs could be quantified by measuring the fluorescence signal of the QDs on the SCCBs. In addition, by utilizing different MoS 2 -integrated structural color encoded SCCBs, multiplexed miRNA quantification could also be realized. Based on this strategy, we have demonstrated that several pancreatic cancer-related miRNAs could be selectivity and sensitivity detected with a detection limit of 4.2 ± 0.3 nM. These features make the MoS 2 -integrated SCCB ideal for many potential applications.
研究领域:
生物医学工程
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纳米技术
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癌症研究
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分子诊断
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2023
·Vol. 11
·期 25
·5856-5869
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IF 7
Fei Zhou,Yang Yang,Yuying Liu,Haotian Deng,Jianhua Rong,Jianhao Zhao
Intracellular calcium ions (Ca2+) influence the proliferation–apoptosis balance, and lactic acidosis is an innate feature of a malignant tumor. In this study, a calcium hydroxide/oleic acid/phospholipid nanoparticle [CUR–Ca(OH)2-OA/PL NP] with lipase/pH dual responsive delivery of Ca2+ and curcumin (CUR) was developed for inducing cancer cell apoptosis by a combination of intracellular calcium overload and lactic acidosis elimination. The nanoparticle showed a core–shell structure with some good performance, including an adequate nano-size, negative charge, good blood circulation stability, and non-hemolysis. MDA-MB-231 breast cancer cells exhibited a higher lipase activity than A549 human lung adenocarcinoma cells and L929 mouse fibroblasts by fluorescence analysis. CUR–Ca(OH)2-OA/PL NPs were highly internalized by MDA-MB-231 cells, intracellularly released CUR and Ca2+, triggered the activation of caspase 3 and caspase 9, and caused apoptosis by intracellular calcium overload via a mitochondrial-mediated pathway. Lactic acid of 20 mM inhibited the apoptosis of MDA-MB-231 cells depending on the glucose insufficiency level, but this inhibition could be eliminated by CUR–Ca(OH)2-OA/PL NPs, leading to nearly complete apoptosis. Herein, CUR–Ca(OH)2-OA/PL NPs are a potential killer of cancer cells with high lipase activity by a combination of intracellular calcium overload and lactic acidosis elimination.
研究领域:
纳米技术
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癌症生物学
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药学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Jing Bao,Changjun Hou,Yanan Zhao,Xintong Geng,Mickey Samalo,Huisi Yang,Minghong Bian,Danqun Huo
In present study, a sensitive and effective electrochemical microRNA (miRNA) sensing platform is successfully developed by integrating gold nanoparticles/polypyrrole-reduced graphene oxide (Au/PPy-rGO), catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) multiple signal amplification strategy. Firstly, Au/PPy–rGO was employed onto a bare GCE by electrodeposition that can greatly enhanced conductivity and effectively immobilize probes. Then, the thiolated capture probes (SH-CP) were self-assembled on the Au/PPy–rGO modified GCE via Au-S bond. The target miRNA triggered the dynamic assembly of the two hairpin substrates (H1 and H2), leading to the cyclicality of the target miRNA and the formation of H1–H2 complexes without the assistance of enzyme. Subsequently, the newly emerging DNA fragment of H2 triggered the HCR when a mixture solution (hairpins H3 and H4) and produced dsDNA polymers. Finally, a substantial amount of methylene blue (MB) as signal indicator was intercalated into the minor groove of the long dsDNA polymers to achieve detected electrochemical signal. The fabricated sensor is able to detect miRNA-16 (model target) with concentration range from 10 fM to 5 nM with a low detection limit (LOD) of 1.57 fM (S/N = 3). Current research suggests that the developed multiple signal amplification platform has a great potential for the applications in the field of biomedical research and clinical analysis.
研究领域:
生物医学工程
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分析化学
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纳米技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
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2022
·Vol. 13
·期 1
·239-250
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IF 4.036
Hao Zhang,Lixian Xu,Xiaoxiao Gu,Dinghua Yu,Shuang Li
The aggregation state of photosensitizers on the surface of bacterial cells is an important scientific problem for antibacterial photodynamic therapy (APDT). High accumulation and high photoactive state maintenance of photosensitizers are the prerequisite of high APDT efficiency. In this study, an amphiphilic di-cationic methylene blue photosensitizer (C12-MB) was synthesized through quaternization, and its structure, interface properties, photophysical properties and antibacterial photodynamic properties were studied. The results showed that C12-MB could reduce 4.27 log10 CFU and 4.8 log10 CFU for P. aeruginosa and S. aureus under irradiation of light at 660 nm, higher than the parent methylene blue. Through a spectroscopic study on photosensitizer adsorption over the bacterial surface, C12-MB can be accumulated with higher concentration, and the photo-active monomer content is 73% and 70% over P. aeruginosa and S. aureus, higher than those of methylene blue: 25% and 49%, respectively. The higher content of non-aggregated photo-active monomer could contribute to higher antibacterial photodynamic efficiency. For C12-MB adsorbed over bacterial surfaces, planar packing inhibition and electrostatic repulsion could contribute to lower C12-MB aggregation, which provides an useful reference for the structural design of high-efficiency photosensitizers.
研究领域:
光动力疗法
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微生物学
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材料科学
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产品关联
DOI
PubMed
高影响因子
OPTIK
·
2019
·
IF 1.914
Ya Nan Wang,Jin Li,Qian Wang
Semiconductor photocatalysts have played an important role in degrading water pollution in recent years. In this paper, the effects of graphene on the structure, morphology, optical properties and photocatalytic activity of ZnO and Ce-doped ZnO were studied. The photocatalysts of ZnO, Ce doped ZnO (ZnCeO), ZnO-Graphene (ZG), Ce doped ZnO-Graphene (ZGCeO) were synthesized via one-step hydrothermal method and were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), ultraviolet visible absorption (UV–vis). The results of XRD show the synthesized samples were all ZnO phases without impurity peaks. The prepared ZnO was hexagonal wurtzite crystal structure. A flower-like ZG and an approximate flower-like ZGCeO were characterized via SEM. The UV–vis spectra shows that the absorption edges have red-shifts and the absorption band broaden in all doped or composite samples, meanwhile, the band gap energy decreased from 3.26 eV to 3.06 eV. In addition, compared with pure ZnO, the photocatalytic activity of ZnCeO, ZG and ZGCeO are improved. Among them, ZGCeO shows superior photocatalytic activity: the degradation rate of organic dye MB was 99.17 % in 90 min under simulated solar irradiation .
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2022
·
IF 3.191
Ying Liang,Qionglin Wang,Xianwei Zhang,Mengxin Zhang,Bang Du,Weyland Cheng,Huanmin Wang,Lifeng Li,Guangjun Hou,Wancun Zhang
A dual isothermal amplification assay with dual fluorescence signal detection strategy, named dual isothermal amplification all-in-one approach, was developed for rapid, one-step, highly sensitive quantification of plasma circulating MYCN copy number of neuroblastoma (NB). The developed strategy consisted of rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP) on a real-time PCR system using highly specific probe, molecular beacon (MB), as detection probe. The developed strategy possessing a broad linear dynamic range of 10 aM to 1 pM for both target gene ( MYCN ) and reference gene ( NAGK ) . The ratio of the MYCN copy number to NAGK copy number (M/N ratio) was detected by the developed approach in cell lines, NB tumor tissues, hepatoblastoma tumor tissues and Wilms’ tumor tissues, to which the M/N ratios were consistent with previous reports. In particular, the M/N ratio in NB clinical tissue specimens and NB plasma specimens detected with the developed approach were in keeping with the standard RT-PCR approach. More importantly, the M/N ratio in NB tissue samples and corresponding plasma samples of NB patients were consistent with each other with a correlation coefficient of 0.9690, indicating that plasma circulating MYCN is a promising indicator for the risk classification of NB.
研究领域:
肿瘤学
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分子生物学
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临床诊断
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DOI
PubMed
高影响因子
ANALYST
·
2019
·Vol. 144
·期 15
·4575-4581
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IF 4.019
Bo Yin,Sha Sha Li,Shao Hua Lu,Jia Ying Mi,Hong Lin Zhai
Although a large number of fluorescent probes have been developed, the simultaneous quantitative analysis of intracellular thiols is still difficult due to the spectral overlap and the complexity of the intracellular environment. In this study, a multi-signal fluorescent probe was employed for the simultaneous quantification of intracellular glutathione (GSH), cysteine (Cys) and homocysteine (Hcy). As the feature variables of the target components, the Tchebichef image moments (TMs) calculated from the grayscale images of the 3D fluorescence spectra were used to establish the quantitative linear models by stepwise regression. The intra-day and inter-day precisions of the proposed method were less than 5.6% and 8.7%, respectively. The recoveries ranged from 97.0% to 105.9%. In addition, the proposed approach was applied to the simultaneous quantitative determination of Cys, GSH and Hcy in the MCF-10A cell (a type of normal cell) and MDA-MB-231 cancer cell. The obtained results indicated that the concentrations of the three thiols in the cancer cell were higher than those in the normal cell. This study not only provides an effective approach for the quantification of multi-target bio-molecules in complicated intracellular environments, but also further extends the applications of multi-signal fluorescent probes, which will promote the design of new multi-signal fluorescent probes.
研究领域:
分析化学
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生物化学
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癌症研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 21
·8952-8968
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IF 4.5
Qi Wenxia,Wang Shenglan,Yang Heng,Luo Tingting,Zhao Feng,Han Jingtian,Zhang Jing
Combination chemotherapy is a crucial therapeutic strategy to improve the therapeutic efficacy of breast cancer in clinic. When combined with photodynamic therapy (PDT), the therapeutic efficacy can be further enhanced not only by PDT-induced phototoxicity, but also by PDT-induced photochemical internalization which facilitates the intracellular delivery and endo/lysosomes escape of drugs, therefore exhibit considerable potential in clinical treatment of breast cancer. Herein, a novel cocktail therapeutic strategy based on albumin-based nanosystem was established by covalently modifying human serum albumin (HSA) with all-trans retinoic acid (RA) and chlorin e6 (Ce6) to form HSA-RA-Ce6 (HRC) conjugate, and using paclitaxel (PTX) to induce self-assembly of HRC to form HRC@PTX nanoparticles (HRC@PTX NPs). The HRC@PTX NPs could amplify the therapeutic efficacy of combination chemotherapy of RA and PTX with Ce6-mediated PDT. The HRC@PTX NPs were characterized with a mean particle size of 174.1 ± 2.1 nm and a slightly negative zeta potential and exhibited good stability and hemocompatibility. The HRC@PTX NPs could promote the cellular uptake of drugs by MCF-7 cells and MDA-MB-231 cells and produce ROS to amplify the synergistic anti-tumor efficacy. The in vitro inhibitory effects on tumor growth investigated on monolayer and three-dimensional tumor spheroids revealed that HRC@PTX NPs exhibited significantly higher anti-tumor activity when exposed to 660-nm laser. These results demonstrated that HRC@PTX NPs could be developed as a promising cocktail therapeutic strategy for synergistic breast cancer therapy. Graphical abstract
研究领域:
肿瘤学
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药学
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纳米技术
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癌症治疗
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
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2021
·
IF 6.4
Manman Sun,Ke Huang,Xueshi Luo,Hong Li
Purpose Bone metastasis is one of the common causes of death relative to breast cancer. However, the evolvement of bone niche in cancer progression remains poorly understood. A three-dimensional (3D) engineered bone matrix was developed as an effective biomimetic model to explore the mechanism relative to bone cancer metastasis. Methods In the study, a 3D engineered bone matrix was developed via cell biomineralization templated by a biomimetic collagen template. The process of bone metastasis relative to breast cancer was investigated by co-culturing breast cancer MDA-MB-231-GFP cells with pre-osteogenic MC3T3-E1 cells on the 3D bone matrix. Results A typical bone matrix was obtained, where mineralized collagen fibers were packed into the bundle to form a 3D engineered bone matrix. As the cancer cells were invading along the way vertical to the alignment of mineralized collagen fiber, the bone matrix gradually became thinner, accompanied with the erosion of Col I and the loss of calcium and phosphorus. As a result, the disassembled structure of mineralized collagen fiber was observed, which may be attributed to osteolytic bone metastasis. Conclusion An engineered 3D bone-like matrix was successfully prepared via cell mineralization, which can act as a model for bone metastasis process. The study revealed mineralized collagen fiber disassembled at nanoscale relative to breast cancer cells.
研究领域:
癌症生物学
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生物医学工程
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Tunmise Ayode Otitoju,Yun Bai,Yaxiong Tian,Zhongmin Feng,Yun Wang,Xia Zhang,Ting Sun
Herein, we present a novel polyvinylidene fluoride/vanadium-titanium dioxide (PVDF-TiO 2 /V) membranes prepared using LBL self-assembly. The membranes were tested for ultrafiltration of bovine serum albumin solution and photodegradation of SMX solution. The neat PVDF membrane was endowed with photocatalytic function due to the synergistic interaction of TiO 2 and V. The structure and morphology of PVDF-TiO 2 /V membranes were characterized using a variety of techniques, showing that TiO 2 /V particles had been successfully integrated on the membrane surface. By increasing the number of TiO 2 /V layers, contact angle tests demonstrated that the membranes hydrophilicity has been significantly improved. The FTIR result demonstrated that the TiO 2 /V was affixed to the surface of the PVDF membrane by interfacial interactions. The thermal property of the PVDF membrane was also improved with the assembly of TiO 2 /V on the membrane surface. The PVDF-TiO 2 /V membranes maintained a high BSA rejection of 99.4 % and FRR of 97.4 % while showing a water flux up to 390.07 kg m -2 h −1 , which is ∼1.5 times higher than that of neat PVDF membrane. Specifically, the membranes were capable of degrading SMX under visible-light. The photocatalytic activity for SMX increased with the number of TiO 2 /V layers, attaining the highest value with four self-assembled TiO 2 /V layers. The reaction rate of the optimum membrane was 11.57-fold compared to the neat membrane under visible-light. Furthermore, 95.9 % degradation efficiency was achieved, even after six cycles. More importantly, BPA, MO, and MB could be photodegraded using the PVDF-TiO 2 /V membranes. Compared to using bulk or powdered photocatalysts, the PVDF-TiO 2 /V membrane is more practical and promising for wastewater remediation due to its many advantages including better long-term stability, ability to endure pollutant degradation, low cost, and environmentally friendly.
研究领域:
环境科学
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材料科学
·
水处理
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Xikun Zhang,He Zhang,Xue Lv,Ting Xie,Junzheng Chen,Di Fang,Shurui Yi
Dye wastewater poses a serious threat to the environment and human health, necessitating sustainable degradation methods. In this study, Na-based Montmorillonite (MMT) was exfoliated using different ionic liquids ([C16MIM][Cl], [C16MIM][BF 4 ], [C16MIM][PF 6 ]), and silver nanoparticles (Ag NPs) were green-synthesized using hydroxypropyl cellulose (HPC). The HPC significantly enhanced the dispersion of MMT in the hydrogel. By introducing lauryl methacrylate (LMA), a hydrophobic associative network was constructed in PAM/LMA/HPC/MMT@ILs&Ag NPs hydrogel. This hydrogel demonstrated outstanding mechanical properties, with a stress of 833.21 kPa, strain of 3300 %, and toughness of 14.36 MJ/m 3 . It also exhibited excellent catalytic activity, with a rate constant of 0.83 min −1 for 4-nitrophenol degradation at 28 °C. The effects of temperature and catalyst concentration on the catalytic reaction were systematically investigated. This study presents a simple green synthesis approach for Ag NPs using HPC, achieving superior mechanical performance and stable MMT dispersion in aqueous solutions.
研究领域:
材料科学
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环境科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）