JOURNAL OF CONTROLLED RELEASE
·
2020
·
IF 7.727
Qingqing Guan,Ruomi Guo,Shihui Huang,Fan Zhang,Jie Liu,Zhiyong Wang,Xi Yang,Xintao Shuai,Zhong Cao
Iron-based nanomaterials as the main ferroptosis-inducing platforms are more promising because iron itself is a key component in the Fenton reaction to produce ROS . However, the Fe dose needs to be very high in order to induce ferroptosis-based cancer treatment using the SPIO NPs. Therefore, it is still of great challenge to enhance the efficacy of ferroptosis-based cancer therapy by associating the iron-based nanomaterials with other components and therapeutic modalities. In this study, sorafenib (SRF) and ultrasmall SPIO nanoparticles were loaded into the mesopores and onto the surface of MPDA NPs to form [email protected] nanoparticles. SPIO loading endowed the system with iron-supply for ferroptosis and made the system MRI-visible. Meanwhile, SRF was able to induce ferroptosis in cancer cells with lower Fe dose. Furthermore, the heat generated by MPDA NPs upon laser irradiation offered a moderate PTT to boost the ferroptosis effect. The [email protected] exhibited biocompatibility highly desirable for in vivo application and superior anticancer therapy via the combination of ferroptosis and photothermal therapy.
研究领域:
纳米技术
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癌症研究
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医学影像
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诊疗一体化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2012
·Vol. 5
·期 2
·744-752
·
IF 5.914
Qian Wan,Lisi Xie,Lin Gao,Zhiyong Wang,Xiang Nan,Hulong Lei,Xiaojing Long,Zhi-Ying Chen,Cheng-Yi He,Gang Liu,Xin Liu,Bensheng Qiu
As a versatile gene vector, minicircle DNA (mcDNA) has a great potential for gene therapy. However, some serious challenges remain, such as to effectively deliver mcDNA into targeted cells/tissues and to non-invasively monitor the delivery of the mcDNA. Superparamagnetic iron oxide (SPIO) nanoparticles have been extensively used for both drug/gene delivery and diagnosis. In this study, an MRI visible gene delivery system was developed with a core of SPIO nanocrystals and a shell of biodegradable stearic acid-modified low molecular weight polyethyleneimine (Stearic–LWPEI) via self-assembly. The Stearic–LWPEI–SPIO nanoparticles possess a controlled clustering structure, narrow size distribution and ultrasensitive imaging capacity. Furthermore, the nanoparticle can effectively bind with mcDNA and protect it from enzymatic degradation. In conclusion, the nanoparticle shows synergistic advantages in the effective transfection of mcDNA and non-invasive MRI of gene delivery.
研究领域:
生物医学工程
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分子生物学
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影像医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2013
·
IF 7.604
Jinbao Qin,Kangan Li,Chen Peng,Xiangxiang Li,Jiaying Lin,Kaichuang Ye,Xinrui Yang,Qingsong Xie,Zunli Shen,Yuqing Jin,Mier Jiang,Guixiang Zhang,Xinwu Lu
Adipose-derived stem cells (ADSCs) exhibit tremendous potential for repair of ischemic diseases. However, studies on the fate, migration, differentiation, and body distribution of the labeled ADSCs are rarely reported. In this study, magnetic iron oxide nanoparticles were designed, synthesized, and coated with meso-2,3-dimercaptosuccinic acid (DMSA) to produce DMSA nanoparticles (DMSA-NPs). The properties, size distribution, and characterization of DMSA-NPs were evaluated. Green fluorescent protein expressing ADSCs (GFP-ADSCs) were obtained and labeled with DMSA-NPs. The viability, cytotoxicity and multi-differentiation capacity of labeled GFP-ADSCs were evaluated in vitro. Labeled and non-labeled GFP-ADSCs were injected into a mouse model of hindlimb ischemia, and 3T magnetic resonance imaging (MRI) was acquired. The synthesized DMSA-NPs efficiently labeled the GFP-ADSCs in vitro and in vivo without affecting cell viability , proliferation, cell cycle, and multi-differentiation capacity. The MRI showed hypointense spots in the labeled GFP-ADSCs that lasted up to 8 weeks. Prussian blue staining and immunofluorescence assay at 4 and 8 weeks indicated that the labeled GFP-ADSCs were in and around the ischemic sites and some differentiated into capillaries. This observation is identical to that seen for transplants of unlabeled cells. Labeled cells were also identified mainly in the liver and spleen, with significantly smaller amounts in the lungs, intestines, heart, and kidney. Developed DMSA-NPs were shown to exhibit a considerable potential for use as nanoprobes for MRI of stem cells, which will enhance our understanding of cell-based therapeutic strategies for ischemic diseases.
研究领域:
医学
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生物技术
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影像学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2017
·
IF 3.887
Ruitao Cha,Juanjuan Li,Yang Liu,Yifan Zhang,Qian Xie,Mingming Zhang
Fe 3 O 4 nanoparticles with ultrasmall sizes show good T 1 or T 1 + T 2 contrast abilities, and have attracted considerable interest in the field of magnetic resonance imaging (MRI) contrast agents. For effective biomedical applications, the colloidal stability and biocompatibility of the Fe 3 O 4 nanoparticles need to be improved without reducing MRI relaxivity. In this paper, star polymers were used as coating materials to modify Fe 3 O 4 nanoparticles in view of their dense molecular architecture with moderate flexibility. The star polymer was composed of a β-cyclodextrin (β-CD) core and poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) arms. Meanwhile, reduced glutathione (GSH), as a model drug, was also associated with the star polymer. Thus, a new platform for simultaneous diagnosis and treatment was achieved. Compared to the Fe 3 O 4 nanoparticles coated with linear polymers, the Fe 3 O 4 nanoparticles coated with star polymers (Fe 3 O 4 @GCP) possessed higher GSH association capacity and better stability in serum-containing solution. GSH could be released from Fe 3 O 4 @GCP nanoparticles in response to pH value of the solution. Since the sulfhydryl group on GSH is able to combine free radicals, Fe 3 O 4 @GCP nanoparticles exhibited less cytotoxicity compared to the Fe 3 O 4 nanoparticles without including GSH. Furthermore, the nanoparticles could also serve as good T 1 MRI contrast agent, and the MRI relaxivity of Fe 3 O 4 @GCP nanoparticles did not decrease after coated with the star polymer. These results indicate that the precisely designed Fe 3 O 4 @GCP nanoparticles could be used as a versatile promising theranostic nano-platform.
研究领域:
纳米技术
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材料科学
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生物医学工程
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磁共振成像
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药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2023
·
IF 3.7
Yang Chen,Dan Wang,Haijun Yang,Kai Li,Yingfeng Wang,Zhongfeng Li
Lanthanide codoped upconversion luminescence nanoparticles (Ln-doped UCNPs) represent a new generation of biological probes for photoluminescence bioimaging applications. However, the strong absorption of short-wave light below 600 nm by tissues necessitates the development of upconversion nanoparticles capable of emitting red light. UCNPs hold great promise as multimodal contrast agents, which could be a promising alternative in a range of medical imaging techniques. Here, we report the fabrication of hybrid nanoparticles, NaYF4:Yb3+/Er3+/Mn2+@PDA-PEG, exhibiting high luminescent emissions and magnetic relaxivities, as potential nanoprobes for dual-modality imaging combining magnetic resonance imaging (MRI) and upconversion luminescence (UCL) imaging. By doping with Mn2+, UCNPs were endowed with MRI performance and tuned with the red/green light ratio of UCL. The biocompatibility and hydrophilicity of the nanoparticles were improved by polymerizing polydopamine (PDA) on their outer surface and grafting onto a poly(ethylene glycol) (PEG) coating, which could further enhance the red-to-green emission ratio. The results show that the Mn2+-doped UCNP@PDA-PEG displays red luminescence and superior MRI contrast enhancement with the longitudinal relaxivity r1 of up to 14.08 mM–1·s–1, thus showing great potential as dual-mode UCL/MRI bioimaging nanoprobes.
研究领域:
生物医学工程
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纳米医学
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医学影像学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2022
·
IF 7.419
Jianfeng Bao,Shuangshuang Guo,Xiangyang Zu,Yuchuan Zhuang,Dandan Fan,Yong Zhang,Yupeng Shi,Xin Pang,Zhenyu Ji,Jingliang Cheng
Nowadays, about 30% of magnetic resonance imaging (MRI) exams need contrast agents (CAs) to improve the sensitivity and quality of the images for accurate diagnosis. Here, a multifunctional nano-agent with ring-like vortex-domain iron oxide as core and gadolinium oxide as shell (vortex nanoring Fe 3 O 4 @Gd 2 O 3 , abbreviated as VNFG) was firstly designed and prepared for highly enhanced T 1 -T 2 dual-modality magnetic resonance imaging (MRI)-guided magnetic thermal cancer therapy. After thorough characterization, the core-shell structure of VNFG was confirmed. Moreover, the excellent heat generation property (SAR=984.26 W/g) of the proposed VNFG under alternating magnetic fields was firmly demonstrated. Furthermore, both in vitro and in vivo studies have revealed a good preliminary indication of VNFG’s biological compatibility, dual-modality enhancing feature and antitumor efficacy. This work demonstrates that the proposed VNFG can be a high-performance tumor diagnosis and theranostic treatment agent and may have great potential for clinical application in the future.
研究领域:
纳米技术
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生物医学工程
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癌症治疗
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磁共振成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
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2020
·
IF 0
Chen Bai,Pengcheng Hu,Nianlong Liu,Guodong Feng,Di Liu,Yi Chen,Ming Ma,Ning Gu,Yu Zhang
To elevate the accurate diagnosis of tumors, nanomaterial-based magnetic resonance imaging (MRI) contrast agents are being rapidly developed. In this paper, we report the large-scale synthetic method of Fe3O4 nanoparticles by high-temperature thermal decomposition and their application as time-dependent T1–T2 dual-modal MRI contrast agents in rabbit hepatic tumors. The Fe3O4 nanoparticles are modified with bull serum albumin (BSA), which provides excellent biocompatibility and colloidal stability. These nanoparticles also exhibit a high r1 value of 6.99 mM–1 s–1 and a low r2 value of 24.11 mM–1 s–1 at a clinically relevant magnetic field (3.0 T). In in vivo experiments, after intravenous administration with these nanoparticles, the rapid T2-weighted effect can be acquired within 5 min and the slow T1 contrast enhancement at the tumor site appears at 90 min. The explanation of this phenomenon is possibly due to the different accumulation rates and states of the nanoparticles in different tissues. In normal liver tissue, the nanoparticles can be quickly phagocytosed by a reticuloendothelial system and accumulated in the liver. These accumulated nanoparticles aggregate, improving the T2-weighted effect and reducing the T1-weighted effect. However, because of an enhanced permeation and retention effect, the nanoparticles are delivered into tumor tissue gradually and dispersedly, which takes a longer time to generate the T1-weighted effect at the tumor site. In summary, these Fe3O4 nanoparticles have significant potential to improve the diagnostic accuracy and sensitivity in MRI.
研究领域:
纳米技术
·
医学成像
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生物医学工程
·
癌症研究
·
药理学
✓ 已验证
产品关联
DOI
Frontiers in Chemistry
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2022
·
IF 5.545
Zhongtao Li,Jing Guo,Mengmeng Zhang,Guohua Li,Liguo Hao
Magnetic resonance molecular imaging can provide anatomic, functional and molecular information. However, because of the intrinsically low sensitivity of magnetic resonance imaging (MRI), high-performance MRI contrast agents are required to generate powerful image information for image diagnosis. Herein, we describe a novel T 1 contrast agent with magnetic-imaging properties facilitated by the gadolinium oxide (Gd 2 O 3 ) doping of mesoporous silica nanoparticles (MSN). The size, morphology, composition, MRI relaxivity ( r 1 ), surface area and pore size of these nanoparticles were evaluated following their conjugation with Gd 2 O 3 to produce Gd 2 O 3 @MSN. This unique structure led to a significant enhancement in T 1 contrast with longitudinal relaxivity ( r 1 ) as high as 51.85 ± 1.38 mM −1 s −1 . Gd 2 O 3 @MSN has a larger T 1 relaxivity than commercial gadolinium diethylene triamine pentaacetate (Gd-DTPA), likely due to the geometrical confinement effect of silica nanoparticles. These results suggest that we could successfully prepare a novel high-performance T 1 contrast agent, which may be a potential candidate for in-vivo MRI.
研究领域:
材料科学
·
纳米技术
·
医学影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Chemistry Frontiers
·
2019
·Vol. 3
·期 7
·1365-1374
·
IF 0
Xuefeng Gao,Yang Liu,Yingying Li,Xiaoyan Niu,Jing Cao,Xiaomin Li,Yapei Zhang,Dongzhi Tan,Wei Wang,Zhi Yuan
Superparamagnetic iron oxides (SPIO) are attractive MRI contrast agents but suffer from limited sensitivity and low tumor specificity. In this study, two sets of SPIO nanoparticles (SPIONPs) were synthesized that were designed to selectively crosslink upon sensing transglutaminase (TGase), which is highly expressed at tumor site, thereby leading to the formation of SPIO nanocluster with T2 signal enhancement properties. For this purpose, SPIONPs with glutamine and lysine surfaces were synthesized. In vitro studies showed that the SPIONPs could crosslink to form aggregates with diameter of 1.3 μm in the presence of TGase and showed a 1.8 fold enhancement in r2 relaxivity compared with the dispersed state. The cellular uptake results illustrated that the SPIONPs could crosslink in high TGase expressing HeLa cells and showed an aggregation-induced retention effect compared with the controlled MCF-7 cell-line (about 1.9 fold). Simultaneously, the administration of the SPIONPs in the HeLa tumor-bearing mice verified the TGase-responsive aggregation and tumor enrichment of the nano contrast agent. MRI imaging also demonstrated the T2 signal enhancing ability of the TGase-responsive SPIONPs; the ΔT2 signal of the SPIONPs in the HeLa tumor-bearing group was about 2.3 fold higher than the MCF-7 group and the TGase-inhibitor treated group.
研究领域:
纳米技术
·
生物医学工程
·
医学成像
·
癌症研究
✓ 已验证
DOI
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Chuyao Chen,Cong Huang,Jiamin Liu,Jia Tao,Yuying Chen,Kan Deng,Yikai Xu,Bingquan Lin,Peng Zhao
The imaging resolution of magnetic resonance imaging (MRI) is influenced by many factors. The development of more effective MRI contrast agents (CAs) is significant for early tumor detection and radical treatment, albeit challenging. In this work, the Hofmeister effect of Fe2O3 nanoparticles within the tumor microenvironment was confirmed for the first time. Based on this discovery, we designed a nanocomposite (FePN) by loading Fe2O3 nanoparticles on black phosphorus nanosheets. After reacting with glutathione, the FePN will undergo two stages in the tumor microenvironment, resulting in the robust enhancement of r1 and r2 based on the Hofmeister effect in the commonly used magnetic field (3.0 T). The glutathione-activated MRI signal of FePN was higher than most of the activatable MRI CAs, enabling a more robust visualization of tumors. Furthermore, benefiting from the long circulation time of FePN in the blood and retention time in tumors, the synergistic therapy of FePN exhibited an outstanding inhibition toward tumors. The FePN with good biosafety and biocompatibility will not only pave a new way for designing a common magnetic field-tailored T1–T2 dual-mode MRI CA but also offer a novel pattern for the accurate clinical diagnosis and therapy of tumors.
研究领域:
医学影像学
·
纳米医学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2022
·
IF 6.064
Song Xianji,Liu Xilin,Ma Yihang,Zhu Qingsan,Bi Mingchao
It is important for future clinical applications to design and synthesize multipurpose scaffolding materials for bone tissue engineering with high osteogenic induction and MRI capability. In the present study, we synthesized Ce/Gd@HA by co-doping Ce3+ and Gd3+ into hydroxyapatite (HA) using a hydrothermal synthesis method, and then Ce/Gd@HA composites were synthesized by combining Ce/Gd@HA nanoparticles with polylactic-co-glycolic acid (PLGA) to investigate whether implanted Ce/Gd@HA/PLGA composites could promote osteoblast viability, leading to tibia repair of the rats and enhance MRI. The measurement results contain X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and environmental scanning electron microscopy (ESEM) showing that HA doped with Ce3+ and Gd3+ was still a hexagonal crystal with high crystallinity. The synthesized Ce/Gd@HA/PLGA composites have a structure and obvious magnetic resonance imaging (MRI) capability. The in vitro experimental results indicated that Ce/Gd@HA/PLGA composites significantly promoted the performance of MC3T3-E1 cells, containing proliferation, adhesion, and osteogenic differentiation capacities. These include the improvement of alkaline phosphatase activity, enhancement of mineral deposition, and upregulation of OCN and COL-1 gene expression. The in vivo experimental results demonstrated that the Ce/Gd@HA/PLGA composites significantly improved the healing rate of rat bone defects. The MRI images indicated that the Ga-doped composites were observed in the MRI T1 sequence in rats. The aforementioned results suggested that Ce/Gd@HA/PLGA composites not only effectively promoted bone formation but also enhanced MRI capability. The composites synthesized in this study have great potential in bone regeneration with an extensive application in bone tissue engineering.
研究领域:
生物材料学
·
组织工程学
·
医学影像学
✓ 已验证
DOI
PubMed
高影响因子
BIOMATERIALS
·
2018
·
IF 8.806
Ruixue Song,Meng Zhang,Yanyan Liu,Zhaowen Cui,Hua Zhang,Zhongmin Tang,Xiaoyan Chen,Haihong Wu,Zhenwei Yao,Mingyuan He,Wenbo Bu
Hypoxia, as an inevitable characteristic of solid tumors, has been regarded as a noticeably causative factor to therapeutic resistance and metastatic variants. Exploring novel theranostics to realize the accurate diagnosis of hypoxia and the simultaneous implementation of effective therapy is a promising prospect for the successful treatment of tumors. In the present study, we designed and synthesized a multifunctional rattle-structured nanotheranostic, with the inner core coated by hollow mesoporous silica for chemical drug Doxorubicin (DOX) storage and hypoxia-sensitive MnO 2 enrichment. In various acidic micro-environments caused by hypoxia, MnO 2 nanosheets could be degraded into manganese ions (Mn 2+ ), which were chelated by the modified Tetraxetanum (DOTA) ligands for real-time T 1 -magnetic resonance imaging (T 1 -MRI), with on-demand DOX release to realize both normoxia and hypoxia-sensitive chemotherapy by overcoming hypoxia. Nanotheranostics integrating hypoxia-driven T 1 -MRI with synergetic chemotherapy have tremendous potential in the intelligent diagnosis, personalized treatment and excellent prognosis of solid tumors in the future.
研究领域:
医学影像学
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）