Polymers
·
2022
·Vol. 15
·期 1
·208
·
IF 4.967
Baojie Zhu,Wenxia Zhang,Jing Zhao,Bilian Chen,Fei Liu,Shaoping Li
Grifola frondosapolysaccharides (GFPs) from different regions in China were characterized and compared using HPSEC-MALLS-RID and saccharide mapping based on HPAEC-PAD analysis for achieving and improving its quality control. The results showed that HPSEC chromatograms and molecular weight distributions of GFPs were similar. The average contents of each polysaccharide fraction (Peaks 1, 2, and 3) showed that Peak 3 was the main component and much higher than the other two polysaccharide fractions, which also contained protein. The result of saccharide mapping showed thatα-1,4-glycosidic,β-1,4-glycosidic and fewβ-1,3-glycosidic linkages were existed in GFPs. The similarity result showed that HPAEC-PAD fingerprints of the oligosaccharide fragments after hydrolysis by endoglycosidase were certainly different, especiallyα-amylase with a mean similar index of only 0.781 ± 0.207. The result of hierarchical cluster analysis (HCA) showed that different batches of GFPs from China can be divided into different clusters. Furthermore, immune-enhancing activity based on RAW 264.7 cells showed significant differences among different GFPs. Based on grey relational analysis (GRA), the fractions of Peak 3 were regarded as the major contributors to its immuno-enhancing activity in GFPs. Overall, the implications from these results were found to be stable, comprehensive, and valid for improving the quality control of GFPs.Keywords:Grifola frondosapolysaccharides;saccharide mapping;HPSEC-MALLS-RID;HPAEC-PAD;immunomodulatory
研究领域:
食品科学与工程
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生物化学
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药理学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Dan Liu,Xinxin Liu,Shuai Huang,Xue Shen,Xiaozhe Zhang,Lihua Zhang,Yukui Zhang
Neurotransmitters (NTs) and neuromodulators (NMs) are two of the most important neurochemicals in the brain, and their imbalances in specific brain regions are thought to underlie certain neurological disorders. We present an on-tissue chemoselective derivatization mass spectrometry imaging (OTCD-MSI) method for the simultaneous mapping of NTs and NMs. Our derivatization system consists of a pyridiniumyl-benzylboronic acid based derivatization reagent and pyrylium salt, which facilitate covalent charge labeling of molecules containing cis-diol and primary amino, respectively. These derivatization systems improved the detection sensitivity of matrix-assisted laser desorption/ionization (MALDI)-MSI and simplified the identification of amino NTs and nucleoside NMs by the innate chemoselectivity of derivatization reagents and the unique isotopic pattern of boron-derivative reagents. We demonstrated the ability of the developed method on brain sections from a hypoxia mouse model and control. The simultaneous imaging of NTs and NMs provided a method for exploring how hypoxic stress and drugs affect specific brain regions through neurotransmitter modulation.
研究领域:
神经科学
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分析化学
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医学
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DOI
PubMed
高影响因子
Quantitative Imaging in Medicine and Surgery
·
2022
·
IF 4.63
Quan Tao,Peiwei Yi,Zimeng Cai,Zelong Chen,Zongwu Deng,Ruiyuan Liu,Yanqiu Feng
Background As an essential physiological parameter, pH plays a critical role in maintaining cellular and tissue homeostasis. The ratiometric chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI) method using clinically approved iodinated agents has emerged as one of the most promising noninvasive techniques for pH assessment. Methods In this study, we investigated the ability to use the combination of two different nonequivalent amide protons, chosen from five iodinated agents, namely iodixanol, iohexol, iobitridol, iopamidol, and iopromide, for pH measurement. The ratio of two nonequivalent amide CEST signals was calculated and compared for pH measurements in the range of 5.6 to 7.6. To quantify the CEST signals at 4.3 and 5.5 parts per million (ppm), we employed two analytic methods: magnetization transfer ratio asymmetry and Lorentzian fitting analysis. Lastly, the established protocol was used to measure the pH values in healthy rat kidneys (n=5). Results The combination of iodixanol and iobitridol at a ratio of 1:1 was found to be suitable for pH mapping. The saturation power level (B 1 ) was also investigated, and a low B 1 of 1.5 µT was adopted for subsequent pH measurements. Improved precision and an extended pH detection range were achieved using iodixanol and iobitridol (1:1 ratio) and a single low B 1 of 1.5 µT in vitro . In vivo renal pH values were measured as 7.23±0.09, 6.55±0.15, and 6.29±0.23 for the cortex, medulla, and calyx, respectively. Conclusions These results show that the ratiometric CEST method using two iodinated agents with nonequivalent amide protons could be used for in vivo pH mapping of the kidney under a single low B 1 saturation power.
研究领域:
医学影像学
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生物医学工程
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DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 8
·1881-1889
·
IF 3.864
Linlin Yang,Jun Zhao,Xinling Yu,Ruilong Zhang,Guangmei Han,Renyong Liu,Zhengjie Liu,Tingting Zhao,Ming-Yong Han,Zhongping Zhang
Hydrogen sulfide (H2S) is a key signaling molecule in the cytoprotection, vascular mediation and neurotransmission of living organisms. In-depth understanding of its production, trafficking, and transformation in cells is very important in the way H2S mediates cellular signal transductions and organism functions; it also motivates the development of H2S probes and imaging technologies. A fundamental challenge, however, is how to engineer probes with sensitivity and cellular penetrability that allow detection of spontaneous production of H2S in the entire cell space and live animals. Here, we report a rationally designed molecular switch capable of accessing all intracellular compartments, including the nucleus, lysosomes and mitochondria, for H2S detection. Our probe comprised three functional domains (H2S sensing, fluorescence, and biomembrane penetration), could enter almost all cell types readily, and exhibit a rapid and ultrasensitive response to H2S (≤120-fold fluorescence enhancement) for the dynamic mapping of spontaneously produced H2S as well as its distribution in the whole cell. In particular, the probe traversed blood/tissue/cell barriers to achieve mapping of endogenous H2S in metabolic organs of a live Danio rerio (zebrafish). These results open-up exciting opportunities to investigate H2S physiology and H2S-related diseases.
研究领域:
细胞生物学
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生物化学
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医学影像学
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2016
·
IF 8.387
Maoquan Chu,Wangxi Hai,Zheyu Zhang,Fangjie Wo,Qiang Wu,Zefei Zhang,Yuxiang Shao,Ding Zhang,Lu Jin,Donglu Shi
The use of non-toxic or low toxicity materials exhibiting dual functionality for use in sentinel lymph node (SLN) mapping and cancer therapy has attracted considerable attention during the past two decades. Herein, we report that the natural black sesame melanin (BSM) extracted from black sesame seeds ( Sesamum indicum L.) shows exciting potential for SLN mapping and cancer photothermal therapy. Aqueous solutions of BSM under neutral and alkaline conditions can assemble into sheet-like nanoparticles ranging from 20 to 200 nm in size. The BSM nanoparticles were encapsulated by liposomes to improve their water solubility and the encapsulated and bare BSM nanoparticles were both non-toxic to cells. Furthermore, the liposome-encapsulated BSM nanoparticles (liposome-BSM) did not exhibit any long-term toxicity in mice. The liposome-BSM nanoparticles were subsequently used to passively target healthy and tumor-bearing mice SLNs, which were identified by the black color of the nanoparticles. BSM also strongly absorbed light in the near-infrared (NIR) range, which was rapidly converted to heat energy . Human esophagus carcinoma cells (Eca-109) were killed efficiently by liposome-BSM nanocomposites upon NIR laser irradiation. Furthermore, mouse tumor tissues grown from Eca-109 cells were seriously damaged by the photothermal effects of the liposome-BSM nanocomposites, with significant tumor growth suppression compared with controls. Given that BSM is a safe and nutritious biomaterial that can be easily obtained from black sesame seed, the results presented herein represent an important development in the use of natural biomaterials for clinical SLN mapping and cancer therapy.
研究领域:
医学
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食品科学
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癌症研究
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纳米技术
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2023
·
IF 9.4
Shigen Bai,Yong Tang,Luoyuan Ruan,Ruixuan Song,Huanjian Chen,Yu Du,Hongyu Lin,Yanru Tang,Yufeng Shan
Herein, we propose a mathematical model and corresponding analytical transformation approach based on the conformal mapping technique and equivalent circuit conversion method to investigate the influence of interdigital parameters such as electrode length ( L e ), width ( W e ), and spacing ( D e ) on the electrochemical performance of in-plane supercapacitors (SCs) fabricated via CO 2 laser processing on polyimide film and encapsulation with PVA/H 3 PO 4 gel electrolyte. We conclude from the experiments that the small gap ensures a compact structure, a high electrode utilization rate, and a short anion/cation transport channel, while the large electrode area generates a sufficient electrostatic double-layer effect to improve areal capacitance. Furthermore, interdigital length and width are particularly sensitive to capacitve performance, implying that electrode area plays a critical role. Eventually, the optimal electrode parameters are 10 mm ( L e ), 1.5 mm ( W e ), and 0.35 mm ( D e ), delivering high areal capacitance (2.43 mF/cm 2 ), relatively low resistance (≈46 Ω) and excellent areal energy density (225 μWh/cm 2 ) and power density (3.65 mW/cm 2 ). Because the experimental values are compatible with the simulation curves and the different percentages between them are controlled within 13 %, the mathematical model has theoretical significance for the design of interdigital electrodes.
研究领域:
材料科学
·
物理化学
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产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2026
·
IF 6.6
Yuming Bao,Yuxiao Zhu,Chen Ling,Ni Yang,Yixuan Song,Bingcai Cai,Qinhua Fan,Jiangang Han
Bowl-shaped pyrolytic carbon showed ultrahigh CEF uptake of 2.046 mmol/g. • N-tuned carbon electronics and high site exposure drive superior performance. • XPS/ ESP/ site-shielding revealed cooperative interactions for CEF adsorption. • Interactions resolved at N-, S-, and O-functional groups of CEF and bNPyC. • CEF removal by bNPyC showed broad pH tolerance, interference resistance, and stability. Pyrolytic carbons (PyCs) hold great potential for removing emerging organic micropollutants such as antibiotics from water. Cephalosporins, which contain β-lactam rings and N/S heterocycles, remain underexplored in their affinity interactions with PyCs. Here, solid, bowl, and hollow PyCs were synthesized via a soft-template method. Among them, bowl-shaped nitrogen-doped PyCs (bNPyC) showed faster kinetics and higher capacity for cefazolin (CEF). Its maximum CEF uptake fitted by the Dubinin–Astakhov model reached 2.046 mmol/g (∼929.93 mg/g), exceeding the controls by 25.7–800.9%, highlighting the contribution of nitrogen doping and high site exposure. CEF adsorption on bNPyC remained stable across pH 2–10, increased with temperature, and was slightly enhanced by tested inorganic salts, and exhibited good tolerance to representative organic interferents. XPS and electrostatic potential analyses showed that N-doping increased surface electronic heterogeneity and that multiple groups of CEF jointly engaged in π–π electron donor–acceptor, hydrogen-bonding, and electrostatic interactions with bNPyC. These cooperative forces contributed to the high adsorption capacity and strong affinity toward CEF. bNPyC also showed high adsorption capacities toward several other tested antibiotics and showed only a 10.8% capacity loss after five regeneration cycles. The findings offer new insights into the structure–affinity relationship in cephalosporin adsorption on PyCs. A bowl-shaped N-doped pyrolytic carbon with highly exposed active sites enables multi-site cooperative interactions for efficient and robust adsorption of cefazolin. Download: Download high-res image (222KB) Download: Download full-size image
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DOI
高影响因子
Advanced Science
·
2021
·Vol. 8
·期 21
·2101876
·
IF 16.806
Ruomei Shao,Chunnan Wang,Jingru Zhao,Hang Yang,Shuqing Sun
In the field of biomimetic electronics, flexible sensors with both high resolution and large size are attracting a lot of attention. However, attempts to increase the number of sensor pixels have been thwarted by the need for complex inner circuits and the resulting interferences with the output. Technological challenges, such as real-time spatiotemporal mapping and long-time reliability, must be resolved for large-scale sensor matrices. This paper reports a simple and robust sensor with an arch-bridge architecture (ABA) to address these challenges. The device, which consists of an anti-icing all-transparent material system, is fabricated by immobilizing ABA ionic arrays on predefined grooves on the substrate. It systematically integrates ABA structure-designing, resistance-position-sensing, and parallel-addressing logic, allowing for an improvement of three orders of magnitude in the scanning speed (million-scale pixels) without logical “diagnose confusion.” In addition, it can withstand 100 000 stretching cycles without functional failure. It is also resistant to interferences from stretching. humidity, wet surfaces, and power lines. The proposed strategy is envisaged to serve as a general solution for high-density, large-area tactile sensors in various applications.
研究领域:
仿生电子学
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柔性电子学
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传感器与传感技术
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人机交互
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可穿戴设备
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DOI
高影响因子
Materials
·
2025
·Vol. 18
·期 12
·2740
·
IF 3.2
Xin Jin,Shanshan Meng,Haoxuan Fu,Qi Zhao,Deli Li,Zhuolin Li,Ye Yang,Yanhai Yang,Jiupeng Zhang,Qingyue Zhou
Warm-mix recycled asphalt (WMA-R) technology for reclaimed asphalt pavement (RAP) significantly reduces energy consumption and environmental pollution while maintaining the performance of asphalt mixtures. Significant progress has been made at home and abroad in evaluating the impact of regenerated asphalt mixtures on the performance of regenerated asphalt. However, the performance improvement of WMA-R depends on the effective diffusion of regenerated agents and their interaction mechanism with aged asphalt, which has not been fully studied. This paper systematically studies the diffusion characteristics of biomimetic-based warm-mix regenerant in aged asphalt and its impact on the high- and low-temperature performance of asphalt mixtures through MD and experimental verification. The results show that biomimetic-based warm-mix regenerant can significantly improve the diffusion performance of aged asphalt. Through the rutting test and low-temperature bending test, the significant improvement of the biomimetic-based warm-mix regenerant in the rutting resistance and crack resistance of asphalt mixtures was verified.
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Yanzhi Xing,Yulong Xu,Yanna Lin,E Shuang,Han Leng,Jianhua Wang,Xuwei Chen
Carbon dots (CDs) have been extensively employed in biomolecule imaging. However, the imaging of biological enzymes with CDs has not been reported, which greatly limits their application in biological imaging. Herein, for the first time, a new type of fluorescent CDs is elaborately designed to realize the direct mapping of alkaline phosphatase (ALP) in cells. The obtained phosphorus and nitrogen co-doped CDs (P, N-CDs) generate specific structures including xanthene oxide and phosphate ester, thereby enabling P, N-CDs to be exclusively cleaved by ALP without auxiliary media. The fluorescence intensity of P, N-CDs can be specifically turned on in the presence of ALP, making them powerful probes for sensitive sensing of ALP activity with a detection limit of 1.27 U·L − 1 . Meanwhile, P, N-CDs possessing electron deficiency structure fulfill sensitive responding to polarity variations. The excellent photo-bleaching resistance and biocompatibility of the P, N-CDs are taken for directly mapping the intracellular endogenous ALP via turned-on fluorescence imaging, as well as real-time monitoring the polarity fluctuation in cells through ratiometric fluorescence imaging. The present work offers a new way to design and synthesize functional CDs for direct imaging of intracellular enzymes.
研究领域:
材料科学
·
生物医学工程
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2025
·
IF 3.3
Ruochen Guo,Rui Lv,Rui Shi,Enhui Wu,Chongqing Ma,Chang Sun,Govinda Mandal,Jian Liu
The choices of matrices and the protocols of sample deposition are critical factors which are convolving with each other in the matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging. Previous reports on MALDI MS matrices only compared their performance in MS signal intensities, and provided the optical microphotos or MALDI MS images of the sample spots, lacking of quantitative evaluation. There is an urgent need to develop multivariate model to evaluate the performance of different combinations between matrices and sample protocols. Herein we report a weighted multivariate model for quantitative profiling combinations of matrices/protocols systematically. Our work includes three types of carbon-based nano matrices, such as two-dimensional graphite oxide (GO) nanosheets, one-dimensional carbon nanotubes (CNTs), and zero-dimensional graphite dots (GDs). Four representative sample protocols for MALDI MSI, including rapid evaporation, seed layer, sandwich, and dry droplet, are examined in our model. We select five important parameters: (1) planar dispersity grading by SEM, (2) planar fluorescence homogeneity, (3) coefficient variations in MS signals, (4) MS signal-to-noise ratio, and (5) planar MS signal heatmap grading, with individually assigned weights according to their significance in MSI performance. Calculation of the radar chart areas based on our model provides quantitative profiles for individual combinations. Among them, the dry droplet protocol using the matrix of GDs offers the largest radar chart area, indicating a favorable combination choice for MSI. This work proposes a multivariate model to systematically compare different combinations of matrices and sample protocols, promising a useful tool for mapping small molecules by LDI MS.
研究领域:
分析化学
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质谱成像
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2023
·
IF 5.4
Wen Liang Lu,Houfang Kuang,Jianyou Gu,Xiaojun Hu,Bo Chen,Yingfang Fan
Objective Perineural invasion (PNI) is associated with local recurrence, distant metastasis, and a poor prognosis in pancreatic cancer. However, rare attempt was made to identified the PNI intraoperative. To facilitate precise R0 excision of the tumor, we planned to develop a fluorescent probe for intraoperative imaging of the PNI using GAP-43 as the target and indocyanine green (ICG) as the carrier. Methods The probe was created by binding peptide antibody and ICG. Its targeting was tested in vitro and in vivo using a co-culture model of PC12 and tumor cells to create an in vitro neural invasion model and a mouse sciatic nerve invasion model. The small animal imaging system and surgical navigation system confirmed the probe's potential clinical applicability. The sciatic nerve damage model was created to confirm the probe's targeting. Results We used the pancreatic cancer samples and the public database to confirm that GAP-43 was preferentially overexpressed in pancreatic cancer, particularly in PNI. PC12 cells showed high GAP-43RA-PEG-ICG probe-specific absorption after being co-cultured with tumor cells in vitro. In the sciatic nerve invasion experiment, animals in probe group displayed a significantly stronger fluorescence signal at the PNI compared to ICG-NP and the contralateral normal nerves groups. Although only 60 % of mice appeared to have R0 resections by the naked eye, small animal imaging systems and surgical fluorescence navigation systems could remove the tumor with R0 precision. The injury model used in the probe imaging experimental trials demonstrated that the probe was specifically targeted to the injured nerve, regardless of whether the injury was infiltrated by a tumor or physical. Conclusion We developed the GAP-43Ra-ICG-PEG, an active-targeting near-infrared fluorescent (NIRF) probe, that specifically binds to GAP-43-positive neural cells in an in vitro model of PNI. The probe efficiently visualized PNI lesions in pancreatic cancer in preclinical models, opening up new possibilities for NIRF-guided pancreatic surgery, particularly for PNI patients.
研究领域:
肿瘤学
·
生物医学工程
·
分子生物学
·
外科肿瘤学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）