APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2020
·Vol. 104
·期 24
·10503-10513
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IF 3.53
Hu Gaowei,Miao Yingjie,Luo Xi,Chu Wenhui,Fu Yongqian
Abstract Cell membranes are a great obstacle for entrance of gene therapeutic agents. Cell-penetrating peptides (CPPs) have been proven as a promising gene delivery tool. However, the early TAT peptide derived from the HIV transcription activator protein has been proven that the sequence contains Furin protease cleaved motifs which limited the TAT application in delivery of exogenous active molecules. In the present study, through the bioinformatics and experimental approach, we have identified a novel CPP derived from the N terminus of VP1 protein of chicken anemia virus (CAV), designated as CVP1-N2, which is rich in arginine residues and contains α-helical structure. Then, the ability of CVP1-N2 cell penetrating was detected using confocal imaging and flow cytometry. FITC-labeled CVP1-N2 peptide could rapidly internalize into different types of live cells with dose dependence and without cytotoxic effects by MTT assay. Surprisingly, CVP1-N2 with a pattern of nuclear sub-location has shown the higher uptake efficiency than TAT. At 10, 1, and 0.1 μM, the mean relative internalization of CVP1-N2 was respectively 1.08-, 12-, and 75-fold higher than that of CVP1, as well as 1.6-, 56-, and 75-fold higher than that of TAT. Moreover, using endocytic inhibitors along with low-temperature stress validated that the CVP1-N2 internalization route is direct translocation pathway. Finally, the capacity of CVP1-N2 for delivery of gene into cells was determined, where it was able to carry red fluorescent protein (RFP) and apoptin genes into cells respectively and induce the apoptosis. All these data indicate that CVP1-N2 could be used as a novel gene delivery vehicle for gene therapy in the future. Key points • 1CVP1-N2 was identified as a novel more efficient cell-penetrating peptide.
• 2. CVP1-N2 localized to the nucleus through the direct transduction pathway.
• 3. CVP1-N2 was able to deliver the apoptin gene into HCT116 cells and induce apoptosis.
研究领域:
分子生物学
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基因治疗
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病毒学
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生物技术
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DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Daqiang Liu,Xingliang Yin,Leilei Li,Chuanwu Chen,Qiu-Xia Lin,Hong-Xin Zhang,Erkang Liu,Jun-Feng Wang
Ammonia is in high demand and widely used, yet its production still predominantly relies on the energy-intensive Haber–Bosch process, highlighting the urgent need for a milder alternative technology. Photocatalytic N2 fixation shows great promise in this regard despite the challenge of developing high-efficiency catalysts. In this study, we successfully prepared micronano spherical Bi2MoO6/Bi2MoO6–x S-Scheme homojunctions using a simple solvothermal-calcination coupling method, which offer unique advantages for nitrogen fixation catalysis: (i) the micronano structure mitigates the agglomeration issue common in nanomaterials, thereby exposing more active sites; (ii) the in situ constructed S-Scheme homojunction features a high-quality surface and interface structure, enhancing the spatial separation and transport of photogenerated charges and preserving strong redox capabilities; and (iii) the oxygen-rich vacancy structure promotes the N2 adsorption and activation. Thanks to those favorable properties, the Bi2MoO6/Bi2MoO6–x (BMO/BMOOV) S-Scheme homojunction demonstrates exceptional ammonia production efficiency, with a NH4+ generation rate of 238.7 μmol g–1 h–1 under simulated sunlight, which is over 5.2 times higher than that of pristine Bi2MoO6 (BMO) and significantly outperforms other reported similar catalytic systems (see Table S1). This work introduces a simple and versatile method for synthesizing homojunctions, potentially inspiring the development of more efficient photocatalysts for nitrogen fixation and beyond.
研究领域:
光催化
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材料科学
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环境化学
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可再生能源
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固氮
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DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Zihao Yang,Chao Feng,Yifan Liu,Yong Yang
The scaling relationship limit poses a significant challenge in single-atom catalysts (SACs) for reactions involving multi-intermediate interactions, such as the electrocatalytic nitrogen reduction reaction (eNRR) for ammonia synthesis. To overcome this limitation, a heteronuclear dual Ru-Fe sites on N,S-codoped Ti 3 C 2 T x nanosheet (referred to as Fe 1 -N^S-Ru 1 /Ti 3 C 2 T x ) with precisely designed asymmetric coordination for eNRR is developed. Advanced characterizations verify the unique asymmetric coordination structure where Ru and Fe atoms are individually coordinated to N and S atoms, respectively, with the two metal centers interconnected via bridging N and S atoms. This catalyst achieves remarkable eNRR performance with an NH 3 yield rate of 32.8 µg h −1 mg −1 cat at −0.55 V and 47.1% Faradaic efficiency at −0.25 V, surpassing its homonuclear analogues by 3.2- and 2.3-fold in activity and ≈3.0-fold in selectivity. Experimental and theoretical studies reveal a synergistic mechanism, in which Ru sites effectively dissociate H 2 O to supply protons while the adjacent Fe sites selectively activate N 2 , effectively decoupling proton supply from N 2 activation but also benefiting the formation of key intermediate * NNH. Additionally, the electronic interaction between Ru and Fe sites also lowers the energy barrier of the rate-determining step, thereby significantly enhancing catalytic activity and selectivity.
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Wenhan Wang,Wenqiang Li,Zijian Wang,Bo Liu,Bing Wang,Jingui Duan,Rongfei Zhou
Carbon dioxide capture is an effective method to mitigate climate change . Highly CO 2 -selective and highly moisture-resistant absorbent is essential for energy-efficient carbon capture by absorption. Herein, a highly CO 2 -selective and high-silica CHA zeolite in a pure lithium form (Li-SSZ-13) was synthesized using a direct synthesis method for the first time. The effects of gel n (SiO 2 )/ n (Al 2 O 3 ) ratio, gel n (SiO 2 )/ n (Li 2 O) ratio and synthesis time on the crystallinity , yield, micropore volume and CO 2 loading of the crystals were systematically investigated. The adsorption isotherms of CO 2 and N 2 were measured for directly synthesized Li-SSZ-13, conventional Na-SSZ-13 and ion-exchanged Li-Na-SSZ-13 zeolites. The dynamic breakthrough adsorption properties were measured for Li-SSZ-13 in comparison with Na-SSZ-13 and Li-Na-SSZ-13. The obtained Li-SSZ-13 exhibited a high CO 2 adsorption capacity of 4.48 mmol/g and a high CO 2 /N 2 IAST selectivity of 310 at 100 kPa and 273 K, which were 45.0 % and 441 % higher than Na-SSZ-13 zeolite, 24.8 % and 179 % higher than Li-Na-SSZ-13 zeolite, respectively. It suggests that directly synthesized Li-SSZ-13 zeolite has great potential for efficient CO 2 capture from flue gas .
研究领域:
材料科学
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环境科学
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DOI
高影响因子
FUEL
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2021
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IF 6.609
Yaqi Wu,Danhua Yuan,Shu Zeng,Liping Yang,Xingzong Dong,Quan Zhang,Yunpeng Xu,Zhongmin Liu
Adsorption based process for CH 4 /N 2 separation is promising and attractive while it remains a challenge endeavor due to the lack of efficient adsorbents. In this work, amine ion-exchanged Y zeolites were developed for CH 4 /N 2 separation. By simple ion-exchanged with tetramethylammonium cation (TMA + ) and choline cation (Ch + ), CH 4 adsorption amounts of the resulting adsorbents were obviously increased while their N 2 adsorption amounts were significantly decreased. Consequently, CH 4 /N 2 separation performances of resulted samples were greatly improved compared to pristine NaY. CH 4 /N 2 selectivities of resulting sample TMAY and ChY were up to 6.32 and 6.50, respectively, at 25 °C and 100 kPa. Monte Carlo calculations were used to study the interaction affinity for CH 4 and N 2 on the adsorbents. Breakthrough experiments were further confirmed the excellent separation performances of TMAY and ChY. The excellent separation performances of the resulting adsorbents verified the efficiency of the simple ion-exchange strategy and the application potential of the adsorbents.
研究领域:
化学工程
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材料科学
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DOI
高影响因子
Advanced Energy Materials
·
2018
·Vol. 8
·期 30
·1801357
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IF 21.875
Xianghong Li,Tingshuai Li,Yongjun Ma,Qin Wei,Weibin Qiu,Haoran Guo,Xifeng Shi,Peng Zhang,Abdullah M. Asiri,Liang Chen,Bo Tang,Xuping Sun
The industrial artificial fixation of atmospheric N 2 to NH 3 is carried out using the Haber–Bosch process that is not only energy-intensive but emits large amounts of greenhouse gas. Electrochemical reduction offers an environmentally benign and sustainable alternative for NH 3 synthesis. Although Mo-dependent nitrogenases and molecular complexes effectively catalyze the N 2 fixation at ambient conditions, the development of a Mo-based nanocatalyst for highly performance electrochemical N 2 fixation still remains a key challenge. Here, greatly boosted electrocatalytic N 2 reduction to NH 3 with excellent selectivity by defect-rich MoS 2 nanoflowers is reported. In 0.1 m Na 2 SO 4 , this catalyst attains a high Faradic efficiency of 8.34% and a high NH 3 yield of 29.28 µg h −1 mg −1 cat. at − 0.40 V versus reversible hydrogen electrode, much larger than those of defect-free counterpart (2.18% and 13.41 µg h −1 mg −1 cat. ), with strong electrochemical stability. Density functional theory calculations show that the potential determining step has a lower energy barrier (0.60 eV) for defect-rich catalyst than that of defect-free one (0.68 eV).
研究领域:
材料科学
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电化学
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催化化学
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纳米科技
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能源化学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Cheng Liu,Mengning Chen,Yueling Chen,Qi Chen,Jionghua Wu,Sen Lin,Ling Wu,Jimmy C. Yu
This work develops a series of bimetallic MIL-68(InFe) MOF nanorods (NM(In 1-x Fe x )) mimicking the nitrogenase for biomimetic photocatalytic N 2 fixation. The partial substitution of In 3+ with Fe 3+ leads to the electron redistribution from In 3+ to Fe 3+ , generating electron-poor In 3+ (In (3+δ)+ ) sites and electron-rich Fe 3+ (Fe (3–δ)+ ) sites as an electron acceptor–donor combination to promote the N 2 activation by a π back-donation mechanism. The smaller size of the nanorods further provides more accessible active sites than the bulk counterpart. In cooperation with the H + released from the H 2 O, the activated N 2 molecules were reduced by photogenerated electrons to give NH 3 . The optimal sample NM(In 0.90 Fe 0.10 ) exhibited the highest NH 4 + production rate of 30.8 µmol·h −1 ·g −1 without any sacrificial agent, attributed to the presence of abundant In/Fe bimetallic sites for N 2 activation and enhanced charge mobility. This work provides new insights into rational design for artificial N 2 fixation systems by mimicking natural nitrogenase.
研究领域:
材料科学
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环境科学
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生物化学
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DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2020
·Vol. 30
·期 51
·2005779
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IF 16.836
Guang-Yao Zhai,Dong Xu,Shi-Nan Zhang,Zhong-Hua Xue,Hui Su,Qiu-Ying Yu,Hong-Hui Wang,Xiu Lin,Yun-Xiao Lin,Lu-Han Sun,Xin-Hao Li,Jie-Sheng Chen
Doping the host materials with either electron-rich heteroatoms or electron-deficient heteroatoms have been applied as a straightforward and main-stream method to modify the electronic structures and boost the electrochemical activity for various reactions, including nitrogen reduction reactions (NRR). As the third type of dopants, isoelectric heteroatoms (exemplified with Si atoms in carbon framework in this work) have been designed as highly efficient active centers for NRR. As the same group element with a different size and electronegativity to carbon support, the isoelectric Si heteroatom (Iso-e Si) creates localized singularities with a lone orbital that can act as an electron trap for pre-adsorbed N 2 molecules through coulomb interaction and thus facilitates the following activation process for NRR. Iso-e Si atom thus functions as a special type of metal-free single atom-based electrocatalyst to largely boost the faradaic efficiency of pristine carbon support for NRR by a factor of 12, giving a remarkably high turnover frequency value of 0.52 h −1 , comparable to atomically dispersed transition metal-based electrocatalysts.
研究领域:
材料科学
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电化学
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产品关联
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2020
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IF 16.683
Renquan Guan,Dandan Wang,Yujun Zhang,Chen Liu,Wei Xu,Jiaou Wang,Zhao Zhao,Ming Feng,Qingkun Shang,Zaicheng Sun
Photocatalytic N 2 fixation (PNF) provides a low-cost route to generate ammonia. The poor conversion efficiency from photon to ammonia seriously hinders the step forward because of the low concentration of N 2 molecules. Herein, we demonstrate a photocatalyst with surface oxygen vacancies (Vo) and a F modified surface to facilitate N 2 adsorption and activation on the surface of catalysts. The Vo site promotes the chemical adsorption of N 2 , electron transfer from catalyst to N 2 . F modification switches the TiO 2 surface properties from hydrophilic to aerophilic, thus facilitating the adsorption of N 2 . Meanwhile, the hydrogen reduction reaction (HER) is suppressed, as protons hard to adsorb on F capped surfaces. The optimal NH 3 production rate can reach 206 μmolh −1 g −1 , which is ∼ 9 times higher than that of pure TiO 2 nanoparticles (∼23 μmolh −1 g −1 ). This report provides a potential strategy to overcome mass transfer limitation and achieve a high conversion efficiency in PNF.
研究领域:
材料科学
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催化化学
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产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2020
·
IF 3.573
Juanjuan Dai,Danyan Xie,Ying Liu,Zhiguo Zhang,Yiwen Yang,Qiwei Yang,Qilong Ren,Zongbi Bao
Supramolecular metal–organic frameworks (SMOFs) are emerging as a new family of porous materials. In this work, a SMOF, [Cu2(ade)4(H2O)2](SiF6)2 (SMOF-SIFSIX-1, ade = adenine) was successfully synthesized from the cationic complex of copper and adenine and hexafluorosilicate anions by a slow diffusion method and was demonstrated for trapping carbon dioxide in preference to methane and nitrogen. With one-dimensional pores of about 3.6 × 3.6 Å to induce their distinguishing interactions with the three components, SMOF-SIFSIX-1 exhibited excellent selective gas sorption behaviors for CO2/CH4 (50:50) and CO2/N2 (15:85) gas mixtures with ideal adsorbed solution theory selectivities of 21.1 and 131.7 at 273 K and 100 kPa pressure, respectively, outperforming most of reported hydrogen-bonded organic frameworks so far. Neutron powder diffraction and DFT-D calculations were also carried out to investigate the CO2 adsorption configuration on SMOF-SIFSIX-1a, which suggested that this framework catch CO2 due to “C···F” and “O···H” supramolecular interactions between CO2 molecules and SMOF-SIFSIX-1a. This study demonstrates that the rarely studied SMOF material shows promising potential in gas adsorption separation.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
Frontiers in Chemistry
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2020
·
IF 3.693
Lai Nanjun,Zhu Qingru,Qiao Dongyu,Chen Ke,Wang Dongdong,Tang Lei,Chen Gang
During CO2 flooding, serious gas channeling occurs in ultra-low permeability reservoirs due to the high mobility of CO2. The chief end of this work was to research the application of responsive nanoparticles for mobility control to enhance oil recovery. Responsive nanoparticles were developed based on the modification of nano-silica (SiO2) by 3-aminopropyltrimethoxysilane (KH540) via the Eschweiler-Clark reaction. The proof of concept for responsive nanoparticles was investigated by FT-IR, 1H-NMR, TEM, DLS, CO2/N2 response, wettability, plugging performance, and core flooding experiments. The results indicated that responsive nanoparticles exhibited a good response to control nanoparticle dispersity due to electrostatic interaction. Subsequently, responsive nanoparticles showed a better plugging capacity of 93.3% to control CO2 mobility, and more than 26% of the original oil was recovered. Moreover, the proposed responsive nanoparticles could revert oil-wet surfaces to water-wet, depending on surface adsorption to remove the oil from the surface of the rocks. The results of this work indicated that responsive nanoparticles might have potential applications for improved oil recovery in ultra-low permeability reservoirs.
研究领域:
石油工程
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材料科学
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DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
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2020
·Vol. 7
·期 15
·2778-2782
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IF 5.958
Ri Han,Shufang Chang,Xiaoxiang Xu
Au nanocrystal anchored In2O3 hollow nanospheres have been fabricated, which show good activity for N2 photofixation into ammonia. Such peculiar hollow microstructures favor light harvesting and N2 chemosorption, both of which are further strengthened in the presence of Au nanocrystals. Charge separation in In2O3 has also been improved by anchoring Au nanocrystals.
研究领域:
材料科学
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催化化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）