FOOD CHEMISTRY
·
2025
·
IF 9.8
Qianjin Li,Yulu Xiao,Qinglong Yuan,Menghua Dou,Jianlin Li
Ochratoxin A (OTA) is a mycotoxin and can threaten human health through the food chains. It is essential to develop an effective OTA analysis method to prevent the contaminated cereals from entering the food chain. Here, an analogue of OTA was selected as the template and 3-aminopropyltriethoxysilicane was used as the functional monomer, to prepare a magnetic molecularly imprinted nanoparticles, which were further mixed with polystyrene and SiO 2 nanoparticles to make magnetic molecularly imprinted inverse opal photonic crystal microspheres (MIP@MIPCMs) by a microfluidic self-assembly strategy. Infrared analysis, scanning electron microscope, transmission electron microscope, and metallographic microscopy techniques have been used to characterize MIP@MIPCMs and its control materials, demonstrating the expected MIP@MIPCMs were synthesized successfully. The imprinting factor of MIP@MIPCMs was tested to be 7.3, and its cross-reactivity factors range from 2.3 to 12.9, demonstrating it has good selectivity and specificity. Using MIP@MIPCMs as the sample pretreatment material to enrich OTA from cereals, the detection limit can reach to 0.48 ng/mL by integrating with HPLC analysis, and the spiked recoveries were 80–104 %, indicating the MIP@MIPCMs have a high-performance enrichment capability.
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ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Yi Liang,Xiaolin Huang,Ruijin Yu,Yaofeng Zhou,Yonghua Xiong
The present study described a novel fluorescence enzyme-linked immunosorbent assay (ELISA) used to detect ochratoxin A (OTA) by using the glucose oxidase (GOx)-mediated fluorescence quenching of mercaptopropionic acid-capped CdTe quantum dots (MPA-QDs), in which GOx was used as an alternative to horseradish peroxidase (HRP) for the oxidization of glucose into hydrogen peroxide (H 2 O 2 ) and gluconic acid . The MPA-QDs were used as a fluorescent signal output, whose fluorescence variation was extremely sensitive to the presence of H 2 O 2 or hydrogen ions in the solution. Under the optimized conditions, the proposed fluorescence ELISA demonstrated a good linear detection of OTA in corn extract from 2.4 pg mL −1 to 625 pg mL −1 with a limit of detection of 2.2 pg mL −1 , which was approximately 15-fold lower than that of conventional HRP-based ELISA. Our developed fluorescence immunoassay was also similar to HRP-based ELISA in terms of selectivity , accuracy, and reproducibility. In summary, this study was the first to use the GOx-mediated fluorescence quenching of QDs in immunoassay to detect OTA, offering a new possibility for the analysis of other mycotoxins and biomolecules.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Junxiang Zhang,Zixin Yuan,Yingli Guo,Baomiao Ding,Xingping Zhang,Xinjun Xu,Hualin Yang
The new probe PP(Fe)@HRP with high catalytic activity was used in immunoassay. • The proposed immunoassay showed excellent sensitivity for detecting OTA. • The proposed immunoassay had fluorescence and absorbance dual-mode. Ochratoxin A (OTA) is a mycotoxin with carcinogenic properties. Thus, it is essential to establish a sensitive method for detecting OTA. In this work, a colorimetric and fluorescence dual-mode OTA immunoassay was established based on PP(Fe)@HRP probe. The PP(Fe)@HRP probe consists of PCN-224(Fe), PtNPs and HRP, all of which possess peroxidase activity. When combined to form PP(Fe)@HRP, the peroxidase activity increased significantly. Then, the new probe was used to oxidize TMB to generate blue signal. Meanwhile, the TMB oxidation product (oxTMB) effectively quenched the fluorescence of GSH-AuNCs to generate fluorescence signal. By modifying antibody onto this probe, linking them to the target OTA, an ultra-sensitive dual-mode OTA immunoassay was achieved. This immunoassay demonstrated excellent sensitivity. The limit of detection (LOD) of colorimetric mode was 0.09 ng/mL, and the LOD of fluorescence mode was 0.02 ng/mL. Besides, the immunoassay had high specificity toward OTA and satisfactory recovery rate in corn samples. Download: Download high-res image (135KB) Download: Download full-size image
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Analytical Methods
·
2026
·
IF 2.6
Ningbo Li,Yongdong Wei,Mengsi Liang,Wenkun Zhang,Jie Qiao
Given that ochratoxin A (OTA) exhibits immunotoxic, teratogenic, and carcinogenic properties, it is essential to monitor its presence in food samples. In the present work, a novel electrochemical aptasensor for OTA detection uses β-cyclodextrin-functionalized graphene (CG) to enhance electron transfer and provide abundant double-stranded DNA (dsDNA) binding sites. The sensor immobilizes OTA aptamers, complementary DNA (cDNA), and ferrocene-labeled dsDNA on a gold nanoparticle-modified electrode. The host–guest interaction between β-cyclodextrin (β-CD) and ferrocene (Fc) stabilizes the sensing layer, while thiolated dsDNA ensures strong attachment to AuNPs. Without OTA, impedance is high and current is low. In the presence of OTA, its high affinity for the aptamer induces conformational changes in dsDNA. Upon applying an oxidative potential, ferrocene is oxidized to Fc+, which, due to reduced hydrophobicity, dissociates from β-CD. This releases dsDNA from the electrode surface, reducing the impedance and enhancing the current response. The sensor demonstrates a robust response across a concentration range of 1 pg mL−1 to 100 ng mL−1, achieving a detection limit of 0.78 pg mL−1. Notably, this method has been successfully applied to detect OTA in wine, yielding high recovery rates of 96.20% to 98.67%. These findings suggest that the MCH/AuNPs/dsDNA/CG/GCE configuration is a promising electrochemical sensor for OTA detection.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 3
·1-10
·
IF 5.7
Tang Juan,Liu Liping,Wang Haiyang,Cheng HongLi,Qin Jiao,Zeng Zhiyao,Lin Youxiu,Tang Dianping,Pu Shouzhi
A flexible photoelectrochemical (PEC) biosensor is proposed for the sensitive detection of ochratoxin A (OTA) based on glucose oxidase (GOx)-encapsulated target-responsive hydrogel, using Fenton reaction-mediated in situ formation of polyaniline (PANI) as signal amplified strategy. The target-responsive DNA hydrogels with high loading capacity can carry a large amount of GOx, which not only avoids laborious labeling process but also enhances the analytical performance. Upon introduction of target molecules, the hydrogel can be opened, and multiple GOx was released, thus producing lots of H 2 O 2 via catalytic reduction of glucose. As a component of the Fenton reagent, H 2 O 2 can react with the Fe 2+ on the graphene oxidase-PAMAM-Fe 2+ (GO-PAMAM-Fe 2+ ) to generate Fe 3+ and ·OH. This in turn can oxidize aniline and generate polyaniline (PANI), resulting in the enhancement of the photocurrent signal of GO-MoS 2 -CdS photoelectrode. The GO-PAMAM-Fe 2+ as the neighborhood component of GO-MoS 2 -CdS-based photoactive material not only can increase the loading amount of Fe 2+ , but also can inhibit the decrease of photocurrent of GO-MoS 2 -CdS by direct modification of Fe 2+ on the photoactive material. Moreover, the high loading capacity of DNA hydrogel can efficiently promote the performance of the PEC biosensor. The PEC biosensor exhibited satisfactory analytical performance for OTA with a linear range of 0.0001–0.1 ng/mL and a low detection limit of 0.05 pg/mL. It presents recommendable specificity, stability, and practical applications. Importantly, the PEC biosensor provides a new concept for construction of PEC biosensing platform. Graphical abstract
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 10
·1877-1887
·
IF 4.3
Wang Yinghui,Yu Ling,Zhang Hui,Zhu Runzhi,Meng Zhe
An accurate and sensitive competitive enzyme-linked immunosorbent assay (ELISA) based on persistent luminescence nanoparticles Zn 2 GeO 4 :Mn 2+ , Eu 3+ (ZGME) was developed for detecting ochratoxin A (OTA), a powerfully toxic mycotoxin usually found in grains. As a signal output element of autofluorescence-free biosensors, ZGME can be integrated into ELISA with glucose oxidase (GOx)-binding OTA molecules due to its excellent pH-responsive persistent luminescence. In the absence of OTA, the OTA-GOx conjugate was captured by the anti-OTA monoclonal antibody (anti-OTA mAb) pre-coated on the 96-well plate. The results indicate a decrease in the pH value of the solution, which triggered the quenching of ZGME luminescence due to GOx-dependent gluconic acid production. The presence of OTA inhibited the binding of OTA-GOx on the plate, thus decreasing the production of gluconic acid and increasing the persistent luminous intensity of ZGME. Under the optimized concentrations of anti-OTA mAb and OTA-GOx, quantitative determination of OTA was achieved by plotting the increase or decrease in persistent luminescence intensity of ZGME at 535 nm. In this study, the linear range was from 0.1 μg L −1 to 63 μg L −1 , and the limit of detection (LOD) was as low as 0.045 μg L −1 . In five food samples (corn grit, brown rice, soybean, rice, and wheat), the results exhibited good stability and repeatability, with a recovery range from 81.3% to 94.4% and a relative standard deviation (RSD) of less than 4.2%. Hence, the established method provides a sensitive, accurate, and autofluorescence-free approach for the determination of OTA in different grain samples.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 415
·期 5
·867-874
·
IF 4.478
Zhang Hong,Wang Yuli,Lin Yingtong,Chu Wenjuan,Luo Zhen,Zhao Mingqin,Hu Jiandong,Miao Xiangmin,He Fan
Ochratoxin A (OTA) poses severe risks to the environment and human health, making the development of an accurate and sensitive analytical method for OTA detection essential. In this study, a catalytic hairpin assembly (CHA)–based Förster resonance energy transfer (FRET) aptasensor was developed to detect OTA using carbon quantum dots (CDs) and 6-carboxy-fluorescein (FAM) as dual signal readout. In the presence of OTA, the aptamer specifically interacted with OTA to release the helper DNA (HP), which could open the hairpin structure of FAM-labeled hairpin DNA 1 (H 1 -FAM) modified on the surface of gold nanoparticles (AuNPs). CHA between H 1 -FAM and hairpin H 2 labeled with CDs (H 2 -CDs) can release HP for the next cycle, resulting in the occurrence of FRET with CDs as the energy donor and FAM as the energy acceptor. According to the ratio of F CDs /F FAM , the proposed aptasensor showed a wide linear range from 5.0 pg/mL to 3.0 ng/mL and a low detection limit of 1.5 pg/mL for OTA detection. Moreover, satisfactory results were obtained for OTA detection in rice, suggesting the potential application of this sensor in food safety analysis.
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JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Meilin Wei,Solairaj Dhanasekaran,Qihao Ji,Qiya Yang,Hongyin Zhang
With the increasing global climate change, ochratoxin A (OTA) pollution in food and environment has become a serious and potential risk element threatening food safety and human health. Biodegradation of mycotoxin is an eco-friendly and efficient control strategy. Still, research works are warranted to develop low-cost, efficient, and sustainable approaches to enhance the mycotoxin degradation efficiency of microorganisms . In this study, the activities of N-acetyl- L -cysteine (NAC) against OTA toxicity were evidenced, and its positive effects on the OTA degradation efficiency of antagonistic yeast, Cryptococcus podzolicus Y3 were verified. Co-culturing C. podzolicus Y3 with 10 mM NAC improved 100% and 92.6% OTA degradation rate into ochratoxin α (OTα) at 1 d and 2 d. The excellent promotion role of NAC on OTA degradation was observed even at low temperatures and alkaline conditions. C. podzolicus Y3 treated with OTA or OTA+NAC promoted reduced glutathione (GSH) accumulation. GSS and GSR genes were highly expressed after OTA and OTA+NAC treatment, contributing to GSH accumulation. In the early stages of NAC treatment, yeast viability and cell membrane were reduced, but the antioxidant property of NAC prevented lipid peroxidation. Our finding provides a sustainable and efficient new strategy to improve mycotoxin degradation by antagonistic yeasts, which could be applied to mycotoxin clearance.
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JOURNAL OF HAZARDOUS MATERIALS
·
2022
·
IF 14.224
Qiaoling Wei,Chenxi Huang,Peng Lu,Xiya Zhang,Yiping Chen
Ochratoxin A (OTA) is a fungal secondary metabolite produced by certain species of Aspergillus and Penicillium , and is generally found in contaminated grains, presenting considerable risks to human and animal health. Poor stability and insufficient miniaturization of equipment are the two main shortcomings of existing optical biosensors . Herein, we proposed a novel strategy where a magnetic metal-organic framework (MMOF) was used f or the pre-enrichment of OTA in complex food matrices through magnetic-solid phase extraction, followed by a competitive optical fiber-mediated homogeneous chemiluminescent immunosensor (OFCI) for specific recognition of OTA in grain samples. A homemade miniaturized portable chemiluminescent device (PCD) was designed and applied as the signal readout. The MMOF performs well on OTA adsorption and its adsorption behavior could be well-described by the pseudo-second-order and Freundlich isotherm models. Compared with other MOF materials, this magnetic UiO-66-NH 2 can simply synthesize, has convenient operation, and has high performance with a superior adsorption efficiency of 94 % within 10 min. The proposed MMOF-OFCI was able to quantify OTA from 0.1 to 100 ng/mL, with the limit of detection of 0.28 ng/mL. This sensor exhibited consistent results in comparison with those obtained by the HPLC-MS on real matrices of wheat and corn, thus making it more suitable for on-site detection.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Mengting Chen,Zexuan Liu,Yinyin Guan,Yuxuan Chen,Weipeng Liu,Yingju Liu
The rational design of high-performance nanozyme is of great significance in biosensors. Here, using zeolitic imidazolate framework-8 (ZIF-8) as the precursor, the Co nanoparticle/N-doped carbon nanotubes (Co/NCNT) with hollow core-shell structure were prepared by high temperature pyrolysis of ZIF-8@zeolitic imidazolate framework-67 (ZIF-8@ZIF-67), where ZIF-8@ZIF-67 was not only functionalized as the source of N and C for growth of NCNTs but also provided the template to form the hollow structures. Encapsulation of Co nanoparticles (NPs) in NCNT can effectively anchor active sites and avoid self-agglomeration. The synergistic effect of Co NPs and NCNT is beneficial to increasing the oxidase-mimicking activity significantly, while the hollow structure promotes the binding of the active sites with the substrate to increase the reaction rates. Hence, the Co/NCNT possessed oxidase-mimicking activity to catalyze colorless 3,3′,5,5′-tetramethylbenzidine (TMB) without H 2 O 2 , while the blue TMB + can also quench the fluorescence of AuAg nanoclusters (NCs) by the internal filter effect. A colorimetric-fluorescence dual-mode immunosensor was established to achieve sensitive detection of ochratoxin A ( OTA) in a linear detection range from 0.001 to 10 μg/L with a low detection limit of 0.21 ng/L (colorimetric) and 0.17 ng/L (fluorescence), respectively. Due to the independence and mutual corroboration of the signals, the dual-mode immunoassay is more accurate and reliable than the single-mode immunoassay for OTA. Significantly, the strategy lays a solid foundation for the design of MOF-based nanozymes and innovative inspiration in food visual analysis.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xiujuan Qiao,Xin Ma,Xiaoyu Ma,Tianli Yue,Qinglin Sheng
The increasing requirement for highly sensitive sensors has promoted the development of new functional nanomaterials and various signal amplification strategies. Due to 2 dimensional (2D) materials’ excellent features of large surface areas, structural diversity, and abundant exposed accessible active sites, 2D MOF nanosheets exhibit potential applications in biosensing. Here, an ultrathin micron-sized MOF sheets with good dispersibility and high stability were prepared. Furthermore, a novel label-free aptasensor for ochratoxin A (OTA) detection had been developed based on 2D MOF nanosheets. This sensing signal was amplified by signal amplification strategies containing DNA walker machine, enrichment of Mb signal and nanomaterials of 2D MOF nanosheets and Ag NPs. With the help of these signal amplification strategies, the detection of OTA was reached with a low detection limit (0.08 fg mL −1 ) and a wide linear rage (0.10 fg mL −1 – 1 μg mL −1 ). Additionally, the aptasensor exhibits excellent OTA sensing performances, for example, good stability, outstanding selectivity, excellent repeatability, and regenerability.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2021
·Vol. 413
·期 11
·3131-3140
·
IF 4.157
Zhao Haiyan,Qiao Xiujuan,Zhang Xuelian,Niu Chen,Yue Tianli,Sheng Qinglin
Simultaneous detection of patulin (PAT) and ochratoxin A (OTA) in food products is in great demand, which can prevent toxins from being exposed to human and animal bodies. However, simultaneous detection of multiple targets still faces a challenge. Herein, we developed a novel electrochemical aptasensor for the simultaneous detection of PAT and OTA in apple juice based on gold nanoparticles decorated black phosphorus (AuNPs-BP) nanomaterial. AuNPs-BP function?/work? as a sensing platform for loading much different electrochemical signal molecules functionalized aptamers. In this context, methylene blue functionalized PAT aptamers (Mb-PAT-aptamers) and ferrocene functionalized OTA aptamers (Fc-OTA-aptamers) have been introduced here to fabricate the aptasensor. Fc close to electrode surface showed a strong signal, whereas Mb was far away from electrode surface so exhibited a weak signal in the absence of OTA and PAT. Two kinds of electrochemical signal changes have been recorded dependent on target of OTA and PAT concentrations. So, simultaneous detection of OTA and PAT is achieved. Under the optimum conditions, using this developed biosensor, PAT and OTA can be quantified at a linearity range of 0.01 × 10 −7 μg·mL −1 ~ 0.10 μg·mL −1 . In addition, it also has good selectivity, stability and repeatability. For the practical application, it shows promising performance for the simultaneous detection of PAT and OTA in apple juice.
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