MOLECULES
·
2024
·Vol. 29
·期 5
·1081
·
IF 4.2
Jieqiong Ding,Jie Su,Binhua Luo,Liqiong Ding
Lycorine is a kind of natural active ingredient with a strong antitumor effect. In this study, folate ligand-conjugated polyethylene glycol-block-poly(l-lactide) (PEG-PLLA) nanoparticles (FA-PEG-PLLA NPs) were designed to deliver lycorine to enhance its anti-glioma activity. The successful preparation of the FA-PEG-PLLA polymer was confirmed by1H-NMR, FT-IR, XRD, TGA, and DSC. The optimal formulation for LYC@FA-PEG-PLLA NPs was determined by response surface analysis as follows: sodium dodecyl sulfate (SDS) of 1%, carrier material of 0.03 g, organic phase volume of 3 mL, and ultrasonic power of 20%. The LYC@FA-PEG-PLLA NPs exhibited an encapsulation efficiency of 83.58% and a particle size of 49.71 nm, demonstrating good stability. Hemolysis experiments, MTT assays, and cell scratch assays revealed excellent biocompatibility of FA-PEG-PLLA and superior anti-glioma activity of LYC@FA-PEG-PLLA NPs compared to the raw drug. Additionally, cell apoptosis assays, ROS experiments, and western blot analysis demonstrated that LYC@FA-PEG-PLLA NPs contributed to cell apoptosis by inducing ROS generation and increasing the expression of NF-κB inhibitory protein IκBα. These results suggested that LYC@FA-PEG-PLLA NPs hold promise for glioma treatment.
研究领域:
药学
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纳米技术
·
肿瘤学
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
LUMINESCENCE
·
2024
·Vol. 39
·期 8
·e4871
·
IF 3.2
Yaru Wang,Chaohai Pang,Xionghui Ma,Mingyue Wang,Xiaopeng Wu,Sixin Liu,Shuhuai Li
Graphene oxide (GO) and copper nanoparticles (Cu NPs) were incorporated to modulate and enhance the fluorescence properties of pegylated graphite phase carbon nitride (g-C 3 N 4 -PEG). Combined with the specific recognition capability of a molecular imprinted polymer (MIP), a highly sensitive and selective fluorescent molecular imprinted probe for dopamine detection was developed. The fluorescent g-C 3 N 4 -PEG was synthesized from melamine and modified with GO and Cu NPs to obtain GO/g-C 3 N 4 -PEG@Cu NPs. Subsequently, MIP was prepared on the surface of GO/g-C 3 N 4 -PEG@Cu NPs using dopamine as the template molecule. Upon elution of the template molecule, a dopamine-specific GO/g-C 3 N 4 -PEG@Cu NPs/MIP fluorescence probe was obtained. The fluorescence intensity of the probe was quenched through the adsorption of different concentrations of dopamine by the MIP, thus establishing a novel method for the detection of dopamine. The linear range of dopamine detection was from 5 × 10 −11 to 6 × 10 −8 mol L −1 , with a detection limit of 2.32 × 10 −11 mol L −1 . The sensor was utilised for the detection of dopamine in bananas, achieving a spiked recovery rate between 90.3% and 101.3%. These results demonstrate that the fluorescence molecular imprinted sensor developed in this study offers a highly sensitive approach for dopamine detection in bananas.
研究领域:
分析化学
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生物传感
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材料科学
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产品关联
DOI
PubMed
高影响因子
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2019
·
IF 2.367
Xin-Cheng Zhong,Wen-Hong Xu,Zi-Ting Wang,Wang-Wei Guo,Jie-Jian Chen,Ning-Ning Guo,Tian-Tian Wang,Meng-Ting Lin,Zhen-Tao Zhang,Yi-Ying Lu,Qi-Yao Yang,Min Han,Dong-Hang Xu,Jian-Qing Gao
Objective: This study was aimed to develop DOX-TPP loaded acetal-PEG-PCCL micelles to improve the clinical efficacy of drug resistance tumor.Significance: Chemotherapy is one of the main treatments for breast cancer but is plagued by multidrug resistance (MDR). DOX-TPP-loaded micelles can enhance the specific concentration of drugs in the tumor and improve the efficacy and overcome MDR.Methods: In this study, DOX-TPP-loaded micelles based on acetal-PEG-PCCL were prepared and their physicochemical properties were characterized. The cellular uptake and ability to induce apoptosis of the micelles was confirmed by flow cytometry in MCF-7/ADR cells. In addition, cytotoxicity of the micelles was studied in MCF-7 cells and MCF-7/ADR cells. Confocal is used to study the subcellular distribution of DOX. Free DOX-TPP or DOX-TPP-loaded acetal-PEG-PCCL micelles were administered via intravenous injection in the tail vain for the biodistribution study in vivo.Results: The diameter of micelles was about 102.4 nm and their drug-loading efficiency is 61.8%. The structural characterization was confirmed by 1H NMR. The micelles exhibited better antitumor efficacy compared to free doxorubicin in MCF-7/ADR cells by MTT assay. The apoptotic rate and the cellular uptake of micelles were significantly higher than free DOX and DOX-TPP. Micelles can efficiently deliver mitochondria-targeting DOX-TPP to tumor cells. The result of bio-distribution showed that the micelles had stronger tumor infiltration ability than free drugs.Conclusions: In this study, mitochondriotropic DOX-TPP was conjugated to the nanocarrier acetal-PEG-PCCL via ionic interaction to form a polymer, which spontaneously formed spherical micelles. The cytotoxicity and cellular uptake of the micelles are superior to free DOX and exhibit mitochondrial targeting and passive tumor targeting, indicating that they have potential prospects.
研究领域:
药学
·
肿瘤学
·
纳米技术
·
药物递送系统
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产品关联
DOI
PubMed
高影响因子
CARBON
·
2022
·
IF 11.307
Keke Kang,Limin Wang,Yajie Ma,Kai Yu,Jingwei Liu,Fengyu Qu,Huiming Lin
Here, Co 9 S 8 / [email protected] ( [email protected] ) nanocomposites are synthesized by a two-step hydrothermal process. Firstly, sulfur-doped carbon dots (S-CDs) are prepared by using 3-mercaptopropionic acid as a precursor. A region with denser –SH group is regarded as nucleation site on S-CDs for the growth of Co 9 S 8 to induce heterostructure. As-synthesized [email protected] possess great harvest in NIR region and hyperthermia (51.5%, 1064 nm) for photothermal (PTT). Besides, hyperthermia can elevate the thermal energy of nanocomposite to assist low-energy NIR-II irradiation (1064 nm, 1.16 eV), realizing the excitation of Co 9 S 8 (1.46 eV) and reactive oxygen species (ROS) generation for photodynamic therapy (PDT). What's more, [email protected] exhibits GOx-like activity that could consume glucose to elevate H 2 O 2 level and inhibit intracellular energy supply. In addition, they also show mimic peroxidase (POD) and catalase (CAT) characterization, and the tandem multi-nanozye activities endue the enhanced chemotherapy (CDT) and PDT. [email protected] reveals the greater ROS generation and nanozyme activity compared with pure Co 9 S 8 @PEG, attributing to the efficient charge separation in heterostructure. The novel biodegradation of [email protected] makes the elimination via feces and urine within 2 weeks. That is associated with PTT/PDT/CDT/starvation therapy to activate immune response for anticancer.
研究领域:
材料科学
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纳米技术
·
生物医学工程
·
癌症治疗
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 48
·7859-7869
·
IF 4.872
Huiyun Wen,Haiqing Dong,Jie Liu,Aijun Shen,Yongyong Li,Donglu Shi
Intelligent nanoparticles are capable of prolonged blood circulation without leakage of the payload and fast drug release upon exposure to environmental stimuli, such as redox stimuli, and therefore are highly desirable for cancer therapy. In this study, polymeric micelles were designed and developed with a hydrophilic poly(ethylene glycol) (PEG) shell and a hydrophobic poly-L-phenylalanine (PPhe) core, linked by a redox cleavable bond, i.e. mPEG-SS-PPhe. The mPEG-SS-PPhe micelles were loaded with the anticancer drug doxorubicin (DOX) and shown an on-demand release profile in the presence of redox agents such as glutathione (GSH). Remarkably, the GSH-triggered micellar dissociation accelerated in vitro release of DOX 4.87 fold faster at 10 mM GSH than that without GSH at 12 h. An enhanced inhibitory effect of DOX-loaded mPEG-SS-PPhe micelles was achieved by improving the intracellular GSH levels. Confocal laser scanning microscopy and flow cytometric analyses of HeLa cells further confirmed that DOX accumulation was accelerated by elevating the extracellular GSH concentrations. In addition, mPEG-SS-PPhe micelles showed excellent biocompatibility on L929 and HeLa cell lines. These redox-sensitive polymeric micelles may provide more possibilities as promising carriers for on-demand drug release in a controlled manner.
研究领域:
纳米技术
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药剂学
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癌症治疗
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药物递送系统
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2019
·
IF 3.621
Heling Li,Jinchi Dai,Qi Xu,Cuifen Lu,Guichun Yang,Feiyi Wang,Junqi Nie,Xingjian Hu,Nianguo Dong,Jiawei Shi
A series of high-strength and hydrolytic degradable POSS-PEG hybrid hydrogels were successfully fabricated by Michael addition reaction of 4-arm-PEG-maleimide with thiol-terminated PEG-functionalized POSS cross-linkers in aqueous phase. The cross-linkers, which are hydrophilic, multifunctional and contain esters, were prepared by linking hydrophilic PEG with different chain length onto hydrophobic POSS and terminating with a thiol group. All the prepared hybrid hydrogels were achieved with a porous structure and high mechanical strength, and the G′ of POSS-PEG 500 hybrid hydrogel reached a maximum of 15,000 Pa, which is much higher than our previous POSS-PEG hybrid hydrogels prepared by monofunctional POSS. The swelling ratio and hydrolytic degradation rate of hybrid hydrogels increased with the increase of PEG chain length. In vitro cell culture showed that all the hybrid hydrogels had good biocompatibility. Thus, these hybrid hydrogels exhibited the potential for tissue engineering scaffolds.
研究领域:
材料科学
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生物医学工程
·
组织工程
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2020
·
IF 7.183
Jing Wang,Yapin Liu,Tao Liu,Xingmin Xu,Yunxia Hu
PSf- b -PEG block copolymers with different PEG contents were synthesized to fabricate the highly perm-selective and anti-fouling ultrafiltration (UF) membranes. PEG homopolymer was also blended into the polysulfone casting solutions to fabricate the control (PSf/PEG blend) membranes. The membrane pore structure , surface properties and separation performances of PSf- b -PEG block copolymer membranes and PSf/PEG blend membranes were investigated to identify the role of PEG block for the pore formation. Our results find that the higher PEG content in the PSf- b -PEG block copolymer and in the PSf/PEG blend system was favorable to form more pores and thinner top skin layer with the slightly larger pore size and the higher MWCO of the fabricated membranes. But under the same content, PEG in the block copolymer could help to generate more pores but with similar pore size than PEG in the blend system during the phase-inversion process, which is because of the micro-phase separation of PSf block and PEG block. The fabricated PSf- b -PEG21 (PSf- b -PEG having 21 wt% PEG) membrane had a high water permeation coefficient of 980.0 ± 33.0 LMH/bar and a high BSA rejection above 98%, succeeding the current perm-selectivity upper-bound of the reported polymeric UF membranes. Antifouling experiments confirmed that the PSf- b -PEG21 membrane exhibited a slow and less flux decline during the fouling stage, but a fast and high flux recovery after the backwash cleaning. Our work provides some insights on the relationship of PSf- b -PEG block copolymer composition and membrane structure-separation properties.
研究领域:
材料科学
·
环境工程
·
化学工程
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产品关联
DOI
高影响因子
ADVANCED MATERIALS
·
2025
·
IF 26.8
Xiang Wu,Fuxiao Wang,Ruiyang Li,Biao Yu,Tianhang Qi,Yunpeng Li,Xiao Chen,Jian Wang,Zhen Geng,Peiran Song,Long Bai,Dongyang Zhou,Hou-Feng Zheng,Qin Zhang,Jiacan Su
Conventional hydrogel drug delivery systems are limited in recapitulating the natural spatiotemporal progression of bone regeneration due to their passive release mechanisms. Here, an enzyme-responsive deoxyribonucleic acid (DNA)-polyethylene glycol (PEG) hydrogel is developed to actively coordinate the sequential processes of angiogenesis, osteogenesis, and mineralization through rational material design. The hydrogel integrates matrix metalloproteinase (MMP)-cleavable peptide-crosslinked PEG networks conjugated with actin-stabilized vascular endothelial growth factor (VEGF)-binding DNA strands, enabling spatiotemporally controlled therapeutic release. Upon implantation, MMPs trigger the hydrogel degradation, releasing VEGF to induce angiogenesis while simultaneously promoting osteogenic differentiation. The actin-stabilized DNA framework maintains structural integrity during this stage, preventing premature phosphate release. Subsequent matrix remodeling liberates nuclease that catalyzes DNA to generate phosphate ions, which synergize with peptides in hydrogels and endogenous calcium to drive mineralization. Molecular dynamics simulations reveal the underlying mechanism of hydrogel-mediated mineralization, demonstrating enhanced calcium phosphate formation. Such temporally controlled cascade significantly improves vascular density, osteogenic marker expression, and mineral deposition compared to controls. This work establishes a bioresponsive platform that dynamically interacts with the biological microenvironment to orchestrate multi-phase bone regeneration, offering new possibilities for complex tissue repair.
研究领域:
生物医学工程
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组织工程
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药物递送系统
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生物材料
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再生医学
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产品关联
DOI
PubMed
高影响因子
ACS Sustainable Chemistry & Engineering
·
2020
·
IF 7.632
Yuhui Xie,Weijie Li,Haowei Huang,Dexuan Dong,Xinya Zhang,Li Zhang,Ying Chen,Xinxin Sheng,Xiang Lu
Phase change materials with desirable light-thermal conversion ability are particularly attractive for solar energy harvesting and storage. Herein, we demonstrate that the combination of efficient light-thermal conversion, excellent thermal property, and reliability can be achieved via the construction of a novel form-stable phase change composite material, that is, Radish@PDA/PEG with a sandwich structure. A 3D porous structure derived from radish via freeze-drying is adopted as the substrate; then, self-polymerization of dopamine is initiated in the surface of Radish to produce a layer of polydopamine (PDA). Finally, polyethylene glycol (PEG) impregnation via vacuum infusion is applied to form the Radish@PDA/PEG composite. These results prove that the incorporation of interlayer PDA can not only improve the strength of the porous structure to protect the PEG from leakage, but also enhance the encapsulation capacity of PEG and light absorption ability of the composite. The preparation method is based on common biomass with green chemicals and thus provides a general way to prepare eco-friendly and biodegradable composite with promising applications in light thermal energy management.
研究领域:
材料科学
·
能源工程
·
环境科学
·
可持续材料
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产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2022
·
IF 10.53
Rui Chen,Liuyong Mao,Christine N. Matindi,Gansheng Liu,Jintao He,Zhenyu Cui,Xiaohua Ma,Kuanjun Fang,Biao Wu,Bhekie B. Mamba,Jianxin Li
An in-situ reaction controlled non-solvent induced phase separation (RC-NIPS) method was developed to tailor the morphology of polyvinyl chloride (PVC)/styrene-maleic anhydride (SMA) grafting polyethylene glycol (PEG) (SMA-g-PEG) blend ultrafiltration (UF) membrane. Herein, PVC and SMA was dissolved in dimethylacetamide (DMAc) solvent to prepare a casting solution (16 wt% polymer concentration) at 50 °C. Subsequently, PEG (20 kDa) as an additive and reactant was added to the casting solution at a mass ratio of PEG to the polymer 1/1 wt/wt. to enhance the hydrophilicity and mechanical elongation of the membrane. Results demonstrated that the casting solution with PVC/SMA = 3/1 wt/wt. was an entirely compatible system. The viscosity of the casting solution with adding PEG gradually increased from 692 mPa s to 1880 mPa s at the initial stage and 4780 mPa s at 42 h, then rose sharply to 27,320 mPa s at 84 h. This behavior was ascribed to the esterification reaction between PEG and SMA, leading to pre-gelation and gelation of the system. Whereafter, SMA-g-PEG graft polymers were obtained. Meanwhile, the degree of grafting (DG) increased from 0.40% at 12 h of the reaction to 2.61% at 42 h. Afterwards, a phase inversion using water as coagulation was used to prepare PVC/SMA-g-PEG blend membrane after a certain time of the esterification reaction. It was found that the morphology of resultant membranes systematically changed from asymmetric structure with a dense top layer and finger-like sublayer at the initial stage to a dense layer with a fully sponge-like structure after 18–42 h reaction. The PVC/SMA-g-PEG membrane obtained after the reaction of 42 h demonstrated better hydrophilicity, high pure water permeance and excellent toughness due to the internal plasticization of water molecules. The membrane also exhibited a high BSA rejection (>95%) and fouling recovery rate (FRR) (85.9%). Our work provides some insights on tailoring the UF membrane structure and simultaneously enhancing permeability, antifouling performance and toughness.
研究领域:
材料科学
·
化学工程
·
膜技术
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产品关联
DOI
高影响因子
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
·
2019
·Vol. 38
·期 1
·1-18
·
IF 5.646
Zheng Ning,Liu Weiqun,Li Bifei,Nie Huifang,Liu Jian,Cheng Yunlong,Wang Jichuang,Dong Haiyan,Jia Lee
Background Sorafenib is approved as a standard therapy for advanced hepatocellular carcinoma (HCC), but its clinical application is limited due to moderate therapeutic efficacy and high incidence of acquired resistance resulted from elevated levels of SDF-1/CXCR4 axis induced by prolonged sorafenib treatment. We previously demonstrated metapristone (RU486 metabolite) as a cancer metastatic chemopreventive agent targeting SDF-1/CXCR4 axis. Therefore, we hypothesized that combining sorafenib with metapristone could synergistically suppress cell proliferation, enhance anti-cancer activity and repress potential drug resistance. Methods Changes in cellular CXCR4 expression by metapristone were analyzed by RT-PCR and western blotting. Effect of combining sorafenib with metapristone on cell viability was examined by MTT assay; combination index value was calculated to evaluate the synergistic effect of combined therapy. To overcome poor pharmacokinetics and reduce off-target toxicity, CXCR4-targeted nanoparticles (NPs) were developed to co-deliver sorafenib and metapristone into CXCR4-expressing HCC in vitro and in vivo; cell proliferation, colony formation and apoptosis assays were conducted; nude mice bearing HCC xenograft were used to examine effects of this therapeutic approach on HCC progression. Results Here we showed metapristone significantly reduced CXCR4 expression in HCC. Combinatory chemotherapy of sorafenib with metapristone synergistically suppressed HCC proliferation and resistance. CXCR4-targeted PEGylated poly (lactic-co-glycolic acid) NPs conjugated with LFC131 (a peptide inhibitor of CXCR4), could deliver more sorafenib and metapristone into HCC via specific recognition and binding with transmembrane CXCR4, and resulted in the enhanced cytotoxicity, colony inhibition and apoptosis by regulating more Akt/ERK/p38 MAPK/caspase signaling pathways. Co-delivery of sorafenib with metapristone by the LFC131-conjugated NPs showed prolonged circulation and target accumulation at tumor sites, and thus suppressed tumor growth in a tumor xenograft model. Conclusions In conclusion, co-delivery of sorafenib and metapristone via the CXCR4-targeted NPs displays a synergistic therapy against HCC. Our results suggest combinational treatment of chemotherapeutics offer an effective strategy for enhancing the therapeutic efficacy on carcinoma, and highlight the potential application of ligand-modified tumor-targeting nanocarriers in delivering drugs as a promising cancer therapeutic approach.
研究领域:
肿瘤学
·
药学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2024
·
IF 4.4
Han Song,Jiasheng Fan,Shengkai Liu,Boyu Chen,Jin Huang,Yujie Fu,Zhiguo Liu
In this study, we synthesized DOX-loaded H-ZIF-8@CaCO 3 -PEG nanocarrier, which combined with calcium overload and photothermal therapy (PTT) for synergistic therapy. The nanocarrier comprised a PEG modified calcium carbonate (CaCO 3 ) shell and a hollow imidazolate zeolite skeleton (H-ZIF-8) core, in which the chemotherapy drug doxorubicin hydrochloride (DOX) and photosensitizer indocyanine green (ICG) were simultaneously co-loaded. In vitro cellular experiments revealed that the lethality of DOX&ICG@H-ZIF-8@CaCO 3 -PEG against tumor cells was significantly higher than that of the single chemotherapy owing to the combination with chemotherapy and photothermal therapy as well as calcium overloading. This drug-loaded nanocarrier exhibits notable characteristics such as exceptional stability, elevated drug loading capacity and efficient release of the loaded drug, which provides a promising perspective for efficient synergistic therapy.
研究领域:
纳米技术
·
药学
·
癌症治疗
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）