JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Xin Xiang,Chuning Dong,Lianbo Zhou,Jun Liu,Zachary M. Rabinowitz,Yuzhao Zhang,Honghui Guo,Feng He,Xingdou Chen,Yunhua Wang,Lina Cui,Xiaowei Ma
Real-time detection of cellular senescence remains a clinical challenge. Here, we aimed to develop a positron emission tomography (PET) imaging probe targeting senescence-associated β-galactosidase (SA-β-Gal), the most widely used biomarker of cellular senescence, and investigate its performance for real-time in vivo quantitative detection of cellular senescence. A stable PET imaging agent [68Ga]Ga-BGal was obtained with a high labeling yield (90.0 ± 4.3%) and a radiochemical purity (>95%). [68Ga]Ga-BGal displayed high sensitivity and specificity for β-Gal both in vitro and in vivo. The reaction and uptake of the probe correlated with the β-Gal concentration and reaction time. In PET imaging, high β-Gal-expressing CT26.CL25 tumors and doxorubicin-treated HeLa tumors showed high signals from [68Ga]Ga-BGal, while a low signal was observed in CT26.WT and untreated HeLa tumors. In summary, we showcased successful PET imaging of senescence in preclinical models using probe [68Ga]Ga-BGal. This finding holds the potential for translating senescence imaging into clinical applications.
研究领域:
医学影像学
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分子生物学
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肿瘤学
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放射学
·
药学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Shaoan Cheng,Yangxi Li,Zhen Yu,Ruonan Gu,Wei Wu,Yuqing Su
Polyethylene terephthalate (PET) accounts for 40 % of discarded plastics, and its recycling products have outstanding environmental protection and energy-saving performance, so how to improve the current low recycling rate of waste PET has caught increasing attention. In this paper, waste PET plastic was depolymerized to prepare terephthalic acid (TPA) as an organic linker to synthesize MOF-5. MOF-5 was applied to the adsorption of tetracycline (TC). The batch experiments exhibited that kinetic and isotherm data best fitted the pseudo-first-order model and Langmuir isotherm model with a maximum adsorption capacity of 2325.55 mg g −1 . The regeneration rate of MOF-5 at the fifth cycle was 85.51 %. X-ray photoelectron spectroscopy (XPS) technique and Fourier Transform Infrared (FTIR) spectroscopy both indicated the main adsorption mechanism of MOF-5 toward TC could be attributed to hydrogen bonding and the ionic interactions between the unsaturated bonds (C = O and O–C = O) in MOF-5 and TC molecules. Alginate hydrogel with 30–150 mg MOF-5 loading provided better adsorption performance toward TC than MOF-5 powder, and 90 mg MOF-5 loaded hydrogel performed the highest removal rate of 66.28 %. This work proposed a novel method using waste plastics as raw materials to synthesize pollutant adsorbent, which had an enlightening effect on pollutant treatment.
研究领域:
环境科学
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材料科学
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化学工程
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废物管理
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Mei Hong,Xiao Ma,Wenyuan Hu,Chen Zhu,Wei Dong,Lumin Chen,Fuping Qian
The issue of air pollution, encompassing airborne bacteria and particulate matter (PM), has emerged as a prominent concern impacting human comfort and health. However, conventional air filtration materials fail to meet the requirements for efficient PM capture or immediate bacteria elimination, thereby heightening the risk of contracting diseases due to pollutant accumulation during filtration. Herein, the hydroxyl group in cellulose acetate (CA) was used as the nucleation site to facilitate the self-assembly of Cu-MOF crystals on PET substrate through hydrogen bonding, and then a layer of PVDF-HFP was electrostatically sprayed to finally obtain the high-efficiency and low-resistance PET/CA-MOF-PH air filtration material. On the one hand, the PET/CA-MOF-PH filter material exhibits outstanding filtration performance (99.82 % for PM1.0, 99.93 % for PM2.5, and 99.99 % for PM10) and a low-pressure drop (62 Pa) due to the highly dispersed and dense micro- and nanostructures of the surface layers of the filter material. On the other hand, benefitting from Cu 2+ in the Cu-MOF crystals, PET/CA-MOF-PH filter material also performs excellently in bacterial killing. The filtration capacity difference between commercial PET fibers and PET/CA-MOF-PH filter media at various wind speeds was further investigated through dynamic particle capture simulation and airflow field distribution analysis. This study thus suggests that PET/CA-MOF-PH with high filter efficiency, low resistance, and excellent antibacterial activity designed by self-assembly/electrostatic spraying strategy is expected to be an ideal material for air filters, and this strategy can guide the rational design of multifunctional novel filter materials suitable for environmental and energy fields.
研究领域:
环境科学
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材料科学
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生物学
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产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Peng Gao,Hui Lv,Shao-Kang Qian,Wen-Huan Qiao,Zhen-Yan Hu,Gui-Ping Cao
Solid acid will be an ecofriendly and efficient catalyst for the hydrolysis of polyethylene terephthalate (PET). Very highly Zr-doped MFI-structured zeolites as catalysts for PET hydrolysis were successfully synthesized for the first time. Comprehensive characterizations confirmed the successful incorporation of Zr into the framework. To comprehend the role of Zr within the framework, density functional calculations were employed to investigate its impact on the surface properties and hydrolysis processes. The results demonstrated that Zr d-orbitals enhance Lewis acidity of the catalyst by contributing to the formation of zeolite’s lowest unoccupied molecular orbitals. The interaction between the model molecule and the catalyst surface was enhanced by doping Zr, leading to electron redistribution toward the surface direction on the carbonyl oxygen of model molecule. Consequently, the carbonyl carbon developed a partial positive charge, and the hydrolysis reaction was promoted. The experimental results validated that the Zr-doped catalyst exhibited a 64% enhancement in the reaction rate.
研究领域:
催化化学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF CATALYSIS
·
2025
·
IF 6.5
Qing Jiang,Yunye Huang,Zhen Lu,Yuanhao Lin,Xiafeng Liao,Danni Tang,Linxi Hou,Longqiang Xiao
Hierarchically porous materials are highly valued for their large surface areas and tunable pore architectures, making them promising heterogeneous catalysts for photoinduced electron transfer-reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. However, the specific influence of pore structure on catalytic performance remains poorly understood. In this study, hierarchically porous conjugated organic polymers (COPs) were synthesized using a silica hard-template method and employed as photocatalysts for PET-RAFT polymerization. Under white LED light irradiation, the effects of template size and concentration on pore structure and photocatalytic performance were systematically investigated. Notably, a silica template with a concentration of 60 mg/mL and a particle size of 300 nm produced a COP with an average pore size of 6.91 nm, which exhibited the best photocatalytic performance. The polymerization rate under these conditions was 2.5 times higher than that of the control. The synergy among micropores, mesopores, and macropores enhanced photocatalytic efficiency by increasing surface area, promoting mass transfer, and improving charge carrier mobility. Spectroscopic and electrochemical analyses further revealed that the optimized pore structure significantly enhances charge carrier dynamics by facilitating charge separation and migration. This structural modulation effectively reduces charge recombination, thereby improving the photocatalytic efficiency in PET-RAFT polymerization. These findings provide valuable insights into the rational design of porous photocatalysts for advanced PET-RAFT systems.
研究领域:
高分子化学
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材料科学
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光催化
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纳米技术
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化学工程
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产品关联
DOI
高影响因子
EJNMMI Radiopharmacy and Chemistry
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2024
·Vol. 9
·期 1
·1-17
·
IF 4.4
Chen Lijuan,Li Xiaochen,Ge Yao,Li Huiqiang,Li Ruili,Song Xiaosheng,Liang Jianfei,Zhang Weifeng,Li Xiaona,Wang Xiaoqi,Wang Yunjuan,Wu Yaping,Bai Yan,Wang Meiyun
Background A novel positron emission tomography (PET) imaging tracer, [ 18 F] SynVesT-1, targeting synaptic vesicle glycoprotein 2 (SV2A), has been developed to meet clinical demand. Utilizing the Trasis AllinOne-36 (AIO) module, we’ve automated synthesis to Good Manufacturing Practice (GMP) standards, ensuring sterile, pyrogen-free production. The fully GMP-compliant robust synthesis of [ 18 F] SynVesT-1 boosting reliability and introducing a significant degree of simplicity and its comprehensive validation for routine human use. Results [ 18 F] SynVesT-1 was synthesized by small modifications to the original [ 18 F] SynVesT-1 synthesis protocol to better fit AIO module using an in-house designed cassette and sequence. With a relatively small precursor load of 5 mg, [ 18 F] SynVesT-1 was obtained with consistently high radiochemical yields (RCY) of 20.6 ± 1.2% (the decay-corrected RCY, n = 3) at end of synthesis. Each of the final formulated batches demonstrated radiochemical purity (RCP) and enantiomeric purity surpassing 99%. The entire synthesis process was completed within a timeframe of 80 min (75 ± 3.1 min, n = 3), saves 11 min compared to reported GMP automated synthesis procedures. The in-human PET imaging of total body PET/CT and time-of-flight (TOF) PET/MR showed that [ 18 F] SynVesT-1 is an excellent tracer for SV2A. It is advantageous for decentralized promotion and application in multi-center studies. Conclusion The use of AIO synthesizer maintains high production yields and increases reliability, reduces production time and allows rapid training of production staff. Besides, the as-prepared [ 18 F] SynVesT-1 displays excellent in vivo binding properties in humans and holds great potential for the imaging and quantification of synaptic density in vivo.
研究领域:
医学影像学
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神经生物学
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放射化学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Yinchuan Pu,Duoduo Huang,Wei Xu
Recently, it is urgent for PET films to demonstrate both superhydrophilicity and superoleophilicity to meet evolving industrial demands in applications such as precision printing and biomedical interfaces. This work developed a superamphiphilic PET surface through synergistic integration of oleophilic/hydrophilic units and micro/nano-structures, utilizing a highly efficient UV-curable technology. Epoxy resin (EP) and hydroxyethyl acrylate (HEA) were utilised as the crosslinking matrix, with carboxylated carbon nanotubes (CNTs) as functional fillers for micro/nano-structures formation. The modified PET films exhibited complete wetting behavior, showing 0° contact angles for both water and diiodomethane, along with a 66.8 % increase in surface energy (81.4 mN/m) compared to unmodified films. The E1H2C3 coating maintained 3B-grade adhesion without compromising tensile strength. Futhermore, the highly conductive CNTs significantly enhanced the film's antistatic properties, ultimately reducing resistivity by 11 orders of magnitude versus pristine samples.
研究领域:
材料科学
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表面工程
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纳米技术
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高分子化学
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生物医学工程
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Quanpei Xie,Shengwu Deng,Yong Wen,Yang Yang,Wenjie Fu,Jinxin Liu,Si Cheng
2D transition metal carbides/nitrides/carbonitrides (MXenes) have received extensive attention in solar seawater desalination due to their hydrophilicity, 100 % photothermal conversion efficiency, and efficient light-trapping by enhancing localized surface plasmon resonance (LSPR). Nevertheless, the easily oxidizable characteristics of MXenes, along with salt accumulation and structural weakness impede their practical application in solar-driven interfacial evaporators. Herein, a hydrophobic MXene@oleylamine/polyethylene terephthalate (MXene@OAm/PET) nonwoven with long-term operational stability is fabricated via firmly chemical cross-linking between oleylamine (OAm) modified MXene (MXene@OAm) and NH 2 -PEG-COOH treated PET nonwoven (PEG-PET). The MXene@OAm endows excellent oxidation resistance and hydrophobicity. The prepared MXene@OAm/PET nonwoven which exhibits broad-spectrum solar absorption and high efficiency, can achieve an average evaporation rate in 3.5 wt% NaCl solution at 1.26 kg·m −2 ·h −1 and no seeable salt deposition during 15 irradiation cycles for 8 h a day indicates its superior salt resistance and long-term stability. Most importantly, after being soaked in 3.5 wt% NaCl solution for 40 days, the evaporation rate of MXene@OAm/PET nonwoven still remains stable at around 1.22 kg·m −2 ·h −1 , indicating the significant potential of this prepared nonwoven for long-term seawater desalination and its suitability for industrial applications. Even in industrial wastewater, the evaporation rate can reach 1.27 kg·m −2 ·h −1 . This work demonstrates a successful strategy for achieving high efficiency, stability, and superior salt resistance, which can be potentially utilized in seawater desalination and wastewater treatment.
研究领域:
材料科学
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环境科学
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产品关联
DOI
高影响因子
IONICS
·
2016
·Vol. 23
·期 4
·929-935
·
IF 2.119
Li Weibiao,Li Xiaozhe,Xie Xiaohua,Yuan Anbao,Xia Baojia
A thin composite separator with polyethylene terephthalate nonwoven membrane as the structural support and polyvinylidene fluoride-hexafluoropropylene as the coating layer for lithium-ion batteries was prepared by a simple dip-coating process. The effect of different drying temperatures on the performance of the composite separator was investigated. The results indicate that 80 °C is the optimal drying temperature, preventing leakage current problems and providing a well-developed porous structure. The drying of the composite separator at 80 °C provides a superior thermal stability, better wettability with electrolyte, higher electrolyte uptake, and ionic conductivity compared to commercially available polypropylene (PP) separator. Furthermore, the electrochemical performance consisting of electrochemical stability, self-discharge, cycle performance, rate performance of the composite separator, and PP were determined. The drying of the composite separator at 80 °C shows almost the same oxidation stability and self-discharge performance, but a better cycling and rate performance than the PP separator.
研究领域:
材料科学
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电化学
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能源存储
·
高分子材料
·
电池技术
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2019
·
IF 3.131
Gaoyang Liu,Jiajun Wang,Wei Wang,Dan Yu
The crude oil leakage pollution has become a serious problem which frequently occurs all over the world. To solve this issue, highly efficient oil-water mixtures separation material requires urgently. Herein, a superhydrophobic PET fabric was developed by using a facile and novel method based on UV induced radical polymerization . The prepared PET fabric showed excellent superhydrophobicity with a water contact angle of 156.3°±2° and anti-fouling property either in air or in oil. This superhydrophobic PET fabric not only can be used as a robust oil absorbing material (48∼73 times of its own weight), but also an outstanding separation oil material with separation efficiency of 98.6%, and still maintains 97% after recycled for 10 times. Additionally, the obtained fabric exhibited self-cleaning property and remains durable anti-fouling performance under harsh environments. In sum, this fluorine-free superhydrophobic PET fabric can be a promising crude oil leakage clean up material with self-cleaning property.
研究领域:
材料科学
·
环境工程
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
STAR Protocols
·
2023
·
IF 0
Jie Xiang,Yiyuan Xia,Shilin Luo,Zhentao Zhang,Keqiang Ye
Summary Alpha-synuclein (α-Syn) positron emission tomography (PET) imaging is a valuable approach for diagnosing and monitoring synucleinopathies-related diseases, such as Parkinson disease. Here, we present a protocol for screening potential α-Syn PET tracers using in vitro and ex vivo approaches. We describe steps for employing recombinant pre-formed fibrils and conducting screening procedures on neuronal models, mouse models, and patients’ brain tissue sections to assess the specificity and selectivity of the candidate compounds. For complete details on the use and execution of this protocol, please refer to Xiang et al. (2023). 1
研究领域:
神经科学
·
分子生物学
·
医学成像
·
诊断
✓ 已验证
DOI
PubMed
Frontiers in Microbiology
·
2021
·
IF 5.64
Xu Jiaqi,Dong Feng,Wu Meixian,Tao Rongsheng,Yang Junjie,Wu Mianbin,Jiang Yu,Yang Sheng,Yang Lirong
Efficient and novel recombinant protein expression systems can further reduce the production cost of enzymes. Vibrio natriegens is the fastest growing free-living bacterium with a doubling time of less than 10 min, which makes it highly attractive as a protein expression host. Here, 196 pET plasmids with different genes of interest (GOIs) were electroporated into the V. natriegens strain VnDX, which carries an integrated T7 RNA polymerase expression cassette. As a result, 65 and 75% of the tested GOIs obtained soluble expression in V. natriegens and Escherichia coli, respectively, 20 GOIs of which showed better expression in the former. Furthermore, we have adapted a consensus “what to try first” protocol for V. natriegens based on Terrific Broth medium. Six sampled GOIs encoding biocatalysts enzymes thus achieved 50–128% higher catalytic efficiency under the optimized expression conditions. Our study demonstrated V. natriegens as a pET-compatible expression host with a spectrum of highly expressed GOIs distinct from E. coli and an easy-to-use consensus protocol, solving the problem that some GOIs cannot be expressed well in E. coli.
研究领域:
生物技术
·
分子生物学
·
蛋白质工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）