ACS Nano
·
2022
·
IF 18.027
Ying Huang,Lei Mu,Xin Zhao,Yong Han,Baolin Guo
Burns are a common health problem worldwide and are highly susceptible to bacterial infections that are difficult to handle with ordinary wound dressings. Therefore, burn wound repair is extremely challenging in clinical practice. Herein, a series of self-healing hydrogels (QCS/OD/TOB/PPY@PDA) with good electrical conductivity and antioxidant activity were prepared on the basis of quaternized chitosan (QCS), oxidized dextran (OD), tobramycin (TOB), and polydopamine-coated polypyrrole nanowires (PPY@PDA NWs). These Schiff base cross-links between the aminoglycoside antibiotic TOB and OD enable TOB to be slowly released and responsive to pH. Interestingly, the acidic substances during the bacteria growth process can induce the on-demand release of TOB, avoiding the abuse of antibiotics. The antibacterial results showed that the QCS/OD/TOB/PPY@PDA9 hydrogel could kill high concentrations of Pseudomonas aeruginosa (PA), Staphylococcus aureus, and Escherichia coli in a short time and showed a bactericidal effect for up to 11 days in an agar plate diffusion experiment, while showing good in vivo antibacterial activity. Excellent and long-lasting antibacterial properties make it suitable for severely infected wounds. Furthermore, the incorporation of PPY@PDA endowed the hydrogel with near-infrared (NIR) irradiation assisted bactericidal activity of drug-resistant bacteria, conductivity, and antioxidant activity. Most importantly, in the PA-infected burn wound model, the QCS/OD/TOB/PPY@PDA9 hydrogel more effectively controlled wound inflammation levels and promoted collagen deposition, vascular generation, and earlier wound closure compared to Tegaderm dressings. Therefore, the TOB smart release hydrogels with on-demand delivery are extremely advantageous for bacterial-infected burn wound healing.
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高影响因子
Journal of Biobased Materials and Bioenergy
·
2019
·Vol. 13
·期 4
·490-497
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IF 0
Liu Luo,Meng Shuaiqi,Wei Ren,Jiang Min,Wang Fang,Nie Kaili,Tan Tianwei
Baeyer–Villiger mono-oxygenases (BVMOs) are enzymes that could insert an oxygen into carbon skeletons adjacent to carbonyl groups to form lactone or ester. Recently, a new method of sebacic acid production from oleic acid based on Pseudomonas fluorescens DSM 50106 (PfBVMO)
catalysis was reported. However, the instable property of PfBVMO limited the further investigation and application of this enzyme. In this study, we aimed to improve the stability of PfBVMO by sitedirected mutagenesis. The sequence of PfBVMO indicates probable factors from the susceptible
oxidizing cysteine residue leading to the instability of PfBVMO. Activity and stability of mutants and the wild-type were characterized to investigate the correlation of cysteine residues and activity. The mutant enzyme with entire cysteine residues replaced by serine residues, showed significantly
higher stability than the wild type. The result would facilitate further work on the enzyme modification and crystal structure determination. Meanwhile, the results of this study provided potential for other enzyme stability evolution.
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PROCESS BIOCHEMISTRY
·
2020
·
IF 2.952
Jian Ou,Xin Yuan,Yu Liu,Panliang Zhang,Weifeng Xu,Kewen Tang
Enzyme immobilization in MOFs offers retained enzyme integrity and activity, enhanced stability, and reduced leaching. In this work, Pseudomonas cepacia lipase (PCL) was successfully immobilized into the Zeolitic imidazolate framework-8 (ZIF-8) by physical adsorption. The amounts of PCL in the immobilized enzyme (PCL@ZIF-8) was determined to be 16.7 % (200.5 mg PCL/g ZIF-8). Furthermore, the immobilized enzyme was applied as efficient bio-catalyst for enantioselective hydrolysis of 2-phenylpropionic acid (2-PPA) ester enantiomers and enantioselective transesterification of 1-phenylethanol enantiomers. The enzymatic activity of the immobilized enzyme was three times more than that of free PCL in enantioselective hydrolysis system, and the enantiomeric excess was maintained above 99 %. There was no significant difference in enzyme activity between immobilized PCL and free PCL in transesterification system. In addition, the immobilized enzyme showed good reusability in both hydrolysis (30.57 % of initial activity, 4 cycle) and transesterification reaction systems (64.38 % of initial activity, 6 cycle).
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Microbial Biotechnology
·
2018
·Vol. 13
·期 1
·107-117
·
IF 3.913
Shiyin Liu,Fei He,Nuoqiao Lin,Yumei Chen,Zhibin Liang,Lisheng Liao,Mingfa Lv,Yufan Chen,Shaohua Chen,Jianuan Zhou,Lian-Hui Zhang
Summary Sexual mating of compatible sporida is essential for Sporisorium scitamineum to form dikaryotic mycelia and then cause infection on sugarcane. Our previous work identified a Pseudomonas sp. ST4 from a soil sample, which showed a promising biocontrol potential by inhibiting the mating of S. scitamineum sporida and hyphal growth. In this study, we set to isolate the active compounds from Pseudomonas sp. ST4 through solid fermentation. High-performance liquid chromatography (HPLC) separation coupling with bioassay showed that Pseudomonas sp. ST4 produced a range of antimicrobial compounds. Two of the major components were purified following acetate extraction, silica gel and HPLC separation. Nuclear magnetic resonance (NMR) and liquid chromatography–mass spectrometry (LC-MS) analysis identified these active compounds are 4-hydroxybenzaldehyde and indole-3-carbaldehyde respectively. Further analysis showed that the former compound only inhibited the hyphal growth of the fungus at a concentration of 3 mM, while the latter interfered the fungal sexual mating at a concentration of 0.6 mM and affected hyphal growth at a concentration of 2 mM. Treatment of corn plants with 3 mM indole-3-carbaldehyde significantly inhibited corn smut infection, with a control rate up to 94%. Further analysis of the structure and activity relationship revealed that indole has a much stronger inhibitory activity against the fungal sexual mating than indole-3-carbaldehyde. The results from this study provide new agents for control and prevention of the sugarcane smut disease, and the active compounds could also be used to probe the molecular mechanisms of fungal sexual mating. Graphical 4-hydroxybenzaldehyde and indole-3-carbaldehyde from Pseudomonas sp. ST4 were isolated and identified, the former of which inhibited hyphal growth of Sporisorium scitamineum at a concentration of 3 mM, and the latter of which interfered the fungal sexual mating at a concentration of 0.6 mM, and affected hyphal growth at a concentration of 2 mM and has a good control efficacy against sugarcane smut.
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高影响因子
BIOFOULING
·
2024
·
IF 2.6
Li-Jun He,Zheng-Hui Qiu,Shao-Xia Ma,Rong-Chang Zeng,Cun-Guo Lin
This work investigated the effect of Desulfovibrio caledoniensis (D. caledoniensis) and Pseudomonas aeruginosa (P. aeruginosa) on the microbiologically influenced corrosion (MIC) behaviour of 70Cu-30Ni alloy using surface analysis and electrochemical techniques. The results demonstrated that the mixed medium containing D. caledoniensis and P. aeruginosa further accelerated the MIC of 70Cu-30Ni alloy compared to the single species medium. The addition of exogenous pyocyanin (PYO) to the D. caledoniensis medium increased the maximum pit depth on 70Cu-30Ni alloy from 5.40 μm to 6.59 μm, and the corrosion current density (icorr) increased by one order of magnitude. From the perspective of bioenergetics and extracellular electron transfer (EET), the comprehensive MIC mechanism of 70Cu-30Ni alloy induced by D. caledoniensis and P. aeruginosa was proposed.
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PubMed
高影响因子
JOURNAL OF DAIRY SCIENCE
·
2025
·Vol. 109
·期 1
·68-82
·
IF 4.4
Junling Peng,Fangqian Yin,Ning Ma,Jianping Wang,Jing Liu
With the widespread usage of quinolones (QN) in treating infectious diseases on farms, their notable toxicity and the emergence of resistance have raised increasing concerns in public health and environmental fields. Here, we report a competitive binding chemiluminescence assay (CLA) that enables the detection of 13 QN in milk. We deploy a specific binding biocomponent, the Pseudomonas quinolone signaling receptor (PqsR), to facilitate sensitivity and group-specificity toward QN. Through structural alignment, molecular docking, and surface plasmon resonance method, we elucidate the mechanism of molecular interaction and the high affinity between PqsR and QN. The PqsR-based CLA demonstrates an ultralow limit of detection for 13 QN ranging from 1.91 × 10 −3 to 6.18 × 10 −2 ng·mL −1 , which complies with the maximum residue limit of QN established in China, the European Union, and the United States. Furthermore, it illustrates spiked recoveries in milk matrices ranging from 70.2% to 97.3%, with coefficients of variation below 12.8%. These findings highlight the potential of the PqsR-based CLA for high-throughput, rapid, and sensitive detection of multi-QN. Exploring the commonality of interactions qualitatively and quantitatively from a structural perspective may pave the way for the discovery of novel group-specific binding materials.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2024
·
IF 2.5
Hongchao Dai,Yanhe Zhang,Zhenbo Xu,Thanapop Stoteyome,Lei Yuan
This study investigated the synergistic effects of lactic acid (0.5%, 1% and 2%) and ultrasound (400 W, 20 kHz) on the inactivation of Pseudomonas fluorescens . Combined ultrasound and 2% lactic acid for 5 min reduced the planktonic cell count below 1.40 log cfu/mL. Ultrasound promoted the inactivation of P. fluorescens biofilms by lactic acid and increased the release of ATP and nucleic acids from bacterial cells by 0.09–0.22 μmol/OD and 0.043–0.113 of OD 260 values, respectively. Results indicated that combined ultrasound and lactic acid can serve as a potential technique to control P. fluorescens biofilms.
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高影响因子
ADVANCED MATERIALS
·
2025
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IF 26.8
Siyuan Qian,Xuan Wang,Yishun Guo,Wenfang He,Jianhua Yang,Hao Chen,Renlong Li,Lili Su,Xinyi Wang,Yi Shao,Bailiang Wang
In the treatment of infectious keratitis, therapeutic strategies often prioritize enhancing bactericidal efficacy. However, endotoxins released from Gram-negative bacteria cause inflammatory reaction, leading to corneal structural damage and scar formation. Given that polymyxin B (PMB) can bind and neutralize lipopolysaccharide (LPS), this study employs large-pore mesoporous silica nanoparticles (lMSNs) grafted with PMB as carriers for cationic antibacterial carbon quantum dots (CQDs) to prepare CQD@lMSN-PMB, which enables synchronous sterilization and endotoxin neutralization. In the acidic infectious microenvironment, the accelerated release of CQDs eliminates 99.88% bacteria within 2 h, effectively substituting immune mediated sterilization. Notably, CQD@lMSN-PMB exhibits exceptional LPS neutralization performance (2.22 µg LPS/mg CQD@lMSN-PMB) due to its high specific surface area. In an infectious keratitis model, inflammation subsides significantly within the first day of CQD@lMSN-PMB intervention and is completely resolved by day 3. By day 2, interleukin-1β, interleukin-6 and tumor necrosis factor-α in CQD@lMSN-PMB group decrease by 86.99%, 91.15%, and 77.56%, respectively, compared to the CQDs-only sterilization group. Ultimately, corneal integrity and transparency are preserved, with suppressed expressions of fibrosis-related factors including matrix metalloproteinase 9, transforming growth factor-β and α-smooth muscle actin. Therefore, this synchronous sterilization and endotoxin neutralization strategy outperforms monotherapy strategies focused solely on sterilization or endotoxin neutralization.
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PubMed
高影响因子
Nano Today
·
2022
·
IF 18.962
Zhaofei Guo,Yining Zhu,Guangsheng Du,Ming Qin,Chunting He,Penghui He,Yuanshuai Song,Wenfei Chen,Shuting Bai,Fuhua Wu,Nan Qiao,Min Jiang,Xianjin Luo,Yuandong Zhang,Tao Gong,Zhirong Zhang,Xun Sun
The complex antigen composition and limited selection of adjuvants restricted the development of Pseudomonas aeruginosa ( P. aeruginosa ) subunit vaccines. The evolvement of multiple serotypes of P. aeruginosa also challenges current vaccine candidates due to their poor cross-protection capacity. Herein, we first report a facile strategy to prepare comprehensive antigens loaded nano-vaccines for the rapid development of a P. aeruginosa vaccine with a highly effective cross-protection capacity. Namely, cytosolic antigens derived from bacterial lysates (BL) of PAO1, a drug-resistant P. aeruginosa , were loaded in the mesoporous silica nanospheres (MSNs), while membrane antigens derived from double-layered membrane vesicles (DMVs) of PAO1 were further fused on MSNs surface. The engineered [email protected] showed a clear core-shell morphology, and it significantly promoted cellular internalization and maturation of dendritic cells without additional adjuvants. After subcutaneous vaccination, [email protected] prepared from PAO1 efficiently migrated to lymph nodes, potently induced the humoral and cellular immune responses with exquisite specificity, and substantially protected mice from the challenge of PAO1 and another drug-resistant P. aeruginosa (PA-XN-1). In summary, the MSNs-based comprehensive antigen delivery system successfully protected mice from different strains of P. aeruginosa infection, providing new insights for treatment of other refractory pathogen infections.
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高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Zheng Qi,Zaihui Huang,Chunguang Liu
More than 95% of the bacteria in environment are viable but nonculturable (VBNC). However, it is difficult to elucidate directly the metabolic characteristics of these VBNC bacteria and the differences between biofilm-VBNC bacteria and planktonic-VBNC bacteria. In this study, VBNC P. aeruginosa induced by chlorine was used to clarify the metabolism characteristics and mechanism of differential metabolism between biofilm-VBNC bacteria and planktonic-VBNC bacteria. Results showed that P. aeruginosa in biofilm state was more likely to enter VBNC state. The mechanisms of differential metabolism were involved in the difference of reactive oxygen species production owing to the protection of extracellular polymers. 15 N and 2 H labeled single-cell Raman spectra directly proved that VBNC state bacteria still maintained low material and energy metabolism, and the metabolic activity of biofilm-VBNC P. aeruginosa was lower than that of planktonic-VBNC P. aeruginosa . GC–MS/MS analysis showed 51 metabolites with significant differences. KEGG analysis showed that the types and contents of extracellular metabolites from P. aeruginosa in VBNC states were significantly lower than those in the culturable state ( p < 0.05), mainly involving in citrate cycle, glutathione metabolism, phenylalanine metabolism, tyrosine metabolism and fatty acid degradation. Also, the contents of most extracellular metabolites from P. aeruginosa in biofilm-VBNC state were lower than those in VBNC planktonic state. The significant differences ( p < 0.05) were mainly involved in alanine, aspartate and glutamate metabolism, glycolysis/gluconeogenesis, D-Alanine metabolism and glycerophospholipid metabolism. The result of this research was favorable to the accurate identification of VBNC bacteria, the health risk assessment and scientific control of harmful VBNC bacteria.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Jiaman Xu,Kaixi Zheng,Zsolt Zalán,András Székács,Krisztina Takács,Hui Shi
Insolated Pseudomonas aeruginosa phage with efficient antibacterial effects, which can target and kill P. aeruginosa in wounds. • Optimized the extraction method of wool keratin to enhanced its piezoelectric effect. • The piezoelectric keratin scaffold produces ROS upon ultrasonic stimulation, conferring broad-spectrum antibacterial activity • Integrated keratin scaffolds and phages into a thermosensitive microneedle array enables multifunctional and controllable drug delivery to infected wounds. The slow healing of chronic wounds affects healthcare systems and increases the risk of wound infection, particularly in cases involving drug-resistant bacteria. A patch capable of preventing wound infection and promoting tissue remodeling holds significant value for wound healing. Here, we propose a novel strategy integrating piezoelectric keratin scaffolds and Pseudomonas aeruginosa -targeting phages into thermosensitive pyramidal microneedle arrays to achieve multifunctional and controllable drug delivery, thereby accelerating the healing of infected wounds. A protein scaffold with piezoelectric properties was constructed based on highly α-helical keratin, and its secondary structure and physicochemical properties were systematically characterized. Under ultrasonic drive, this scaffold generates reactive oxygen species via electrical stimulation, achieving broad-spectrum antimicrobial activity. Simultaneously, Pseudomonas aeruginosa phage was incorporated into a temperature-responsive PNIPAm/PAAm blend, enabling controlled release at specific temperatures for targeted antimicrobial effects. The combination of piezoelectric materials and phages exhibits enhanced combinatorial effect, integrating the advantages of physical antimicrobial action with biologically targeted therapy. This strategy offers a novel approach to addressing antibiotic resistance and bacterial resistance to phages, demonstrating potential applications in both antimicrobial materials and phage therapy. Scheme 1. (A) Preparation of wool keratin scaffold. (B) Preparation of microneedles. (C) Schematic of microneedle therapy for P. aeruginosa infection, combining ultrasound-activated keratin ROS production and phage-targeted bacterial elimination. Download: Download high-res image (83KB) Download: Download full-size image
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高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Shichao Peng,Hang Zhai,Yunyun Tan,Yang Liu,Junliang Chen,Fan'ge Kong
Pseudomonas tolaasii , a widely distributed pathogenic bacterium, causes brown blotch disease of edible mushrooms, limiting the advancement of the edible mushroom industry in China. A one-tube method platform that combined clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) system and recombinase-aided amplification (RAA) for P. tolaasii detection was developed. The sub-optimal protospacer adjacent motif (PAM) sequence was selected for the recognition of target DNA to achieve the one-tube detection. Additionally, fluorescent DNA probe and lateral-flow dipsticks (LFD) were utilized for visualization. The established one-tube detection method enabled visual detection, achieving detection limits of 1.6 × 10 −6 ng/μL and 1.6 × 10 −5 ng/μL P. tolaasii recombined plasmid, respectively, by integrating LFD or fluorescent ssDNA probe. It exhibited good specificity, successfully recognizing the P. tolaasii from other 5 common bacteria, including P. aeruginosa , Staphylococcus aureus , Escherichia coli , Brevundimonas di min uta , and Bacillus thuringiensis. The detection time was approximately 25 min. Overall, a CRISPR-coupled RAA method employing suboptimal PAM sequences was developed for one-tube, visual detection of P. tolaasii with high sensitivity and specificity, enabling visual naked-eye readout. The developed method offers a convenient and user-friendly tool for the early diagnosis of P. tolaasii -related diseases in edible mushroom cultivation.
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高影响因子
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