Regenerative Biomaterials
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2026
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IF 8.7
Wei Nannan,Zhang Yuchen,Tian Xinyu,Yao Linyan,Xiao Jianxi
Sustained ultraviolet (UV) irradiation upregulates matrix metalloproteinases (MMPs) activity, exacerbates collagen degradation, and triggers inflammatory signaling, leading to extracellular matrix (ECM) disorganization and skin photoaging. While animal-derived collagens are widely used in clinical applications, their immunogenicity and batch variability limit safety and consistency. Recombinant collagen provides a safer and more controllable alternative. However, poor fibrillogenesis, limited structural stability, and rapid enzymatic degradation hinder long-term performance. Here, we developed an injectable recombinant collagen nanofiber implant (B-SARCI) through the synergistic integration of molecular self-assembly and mild 1,4-butanediol diglycidyl ether (BDDE) crosslinking. This dual strategy preserves excellent injectability while simultaneously enhancing thermal stability, and enzymatic resistance. B-SARCI improved fibroblast attachment and proliferation, promoted migration, and facilitated differentiation in vitro. In a murine UV-induced photoaging model, B-SARCI restored dermal density, enhanced barrier function, and reduced transepidermal water loss (TEWL). Transcriptomic and histological analyses revealed upregulation of Col1α1 and Col3α1, suppression of MMP2, MMP3, and MMP9, and modulation of the JAK-STAT pathway via SOCS3 induction and IL-6 downregulation. Together, these findings demonstrate that B-SARCI re-establishes ECM homeostasis and attenuates inflammation, representing a durable, bioactive, and clinically translatable recombinant collagen implant for photoaged skin repair.
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DOI
PubMed
高影响因子
Regenerative Biomaterials
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2026
·
IF 8.7
Wu Zugui,Huang Xiuhong,Li Jiao,Zheng Gaoquan,Peng Feng,Dong Wei,Zhu Yue,Zhang Yu,Yuan Rong,Wu Zhiwei,Guo Ying,Xiao Jin,Wan Biaolin
Osteoarthritis (OA) involves synergistic pathological changes in both cartilage degradation and subchondral bone remodeling due to oxidative stress, yet current therapies typically target these processes separately. To address this limitation, we developed a dual-functional nanogel (MBG@Pae@HA) by conjugating hyaluronic acid (HA) and paeoniflorin (Pae) to mesoporous bioactive glass nanoparticles via amide bonding and non-covalent interactions, respectively. Leveraging the inherent hydrophilicity of HA, MBG@Pae@HA spontaneously forms nanogels and shows enhanced adhesion force which ensure extended Pae and bioactive ions release in the OA microenvironment. MBG@Pae@HA exhibits potent reactive oxygen species (ROS)-scavenging capacity, contributing to the homeostatic balance between cartilage matrix anabolism and catabolism. Transcriptomic analysis revealed that MBG@Pae@HA treatment reactivates chondrocyte function through activating the cAMP/PKA/CREB antioxidant pathway. Furthermore, with controlled release of Pae and bioactive components (including Ca ions, Si ions, and PO43-) it significantly promotes osteogenesis of bone stem cells. Medial meniscotibial ligament (DMM) model demonstrated the dual therapeutic efficacy of MBG@Pae@HA nanogel with histological and micro-CT analyses confirming concurrent protection against cartilage degradation and enhancement of subchondral bone regeneration. By simultaneously addressing both major pathological features of OA through microenvironment remodeling and prolonged drug delivery, this HA-functionalized and Pae-loaded nanogel represents a significant advance in OA treatment strategies, offering a comprehensive approach to halt disease progression and promote joint repair.
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DOI
高影响因子
Regenerative Biomaterials
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2026
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IF 8.7
Zhang Renjie,Jia Qingqing,Wang Jiahao,Li Wei,Chen Jiang,Hong Wenxin,Huang Huiying,Lin Quankui
Cataract is the leading cause for blindness. While the intraocular lens (IOL) implantation is the only effective treatment in cataract surgery. Posterior capsule opacification (PCO) is the most frequent complication after cataract surgery, mainly resulting from the proliferation and migration of residual human lens epithelial cells (HLECs) within the capsular bag. While surface modification of IOL has helped slow its progression, long-term effectiveness and biocompatibility remain major challenges. Supraphysiological concentration of reactive oxygen species (ROS) induced by natural enzyme stimulation can effectively inhibit proliferation and migration of HLECs, but the effect is limited by enzyme instability. Gold nanoparticles (AuNPs), a promising candidate with enzyme-mimicking activity and desirable photothermal effects, can promote ROS generation and enhance cell apoptosis. To prevent AuNPs aggregation, Fe3+ containing MIL metal-organic frameworks (MOFs) were used as a supporting framework to obtain AuNPs@MIL, which was then modified onto IOL material via electrostatic layer by layer (LbL) self-assembly technique to improve stability and increase immobilization efficiency. The cascade catalytic process, initiated by AuNPs@MIL, can catalyze glucose and oxygen oxidation to produce gluconic acid and hydrogen peroxide. In the formed acidic environment, AuNPs, along with Fe3+ from MIL, convert H2O2 into ·OH via oxidase-like and Fenton-type reactions, which will accelerate cell apoptosis. Additionally, NIR-induced photothermal therapy (PTT) of AuNPs further eliminates abnormally proliferating cells. In vitro and in vivo studies demonstrate that AuNPs@MIL modified IOL offers effective PCO prevention effect, good biocompatibility and excellent optical properties, presenting a viable solution for long-term, efficient PCO prevention.
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DOI
高影响因子
Regenerative Biomaterials
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2026
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IF 8.7
Zhang Jinniu,Zeng Yixing,Huang Yun,Zhu Chunyun,Shen Wenhao,Miao Yingjing,Feng Nianping,Ci Tianyuan
Acute pneumonia is a severe pulmonary inflammation and it is critical to promptly suppress the dysregulated inflammatory responses to prevent mortality. Glucocorticoids are the first-line therapeutic drugs but with poor tissue selectivity and dose-dependent adverse effects. In this work, cryo-leukocyte, an autologous cell-derived immunosuppressor, was created leveraging the cryo-shocking technology by the quick shock of normal leukocytes with liquid nitrogen. After coupling with aICAM-1 functionalized liposomes, this micro/nano composite system could achieve efficient and prompt inflammation alleviation in acute pneumonia. The engineered cryo-leukocytes were of well biocompatibility after evaluation of blood toxicity, tissue toxicity, acute toxicity and long-term biosafety for over six months, etc. Cryo-leukocytes preserved similar cellular receptors as normal leukocytes, capable of recognizing and binding inflammatory cytokines but without activation of immune cascade, thus exhibiting obvious anti-inflammation efficacy by acting as “mixed cytokines antibodies”. The immunosuppression efficacy of cryo-leukocytes was also superior than that of its sub-group cells of cryo-neutrophil, cryo-monocyte and cryo-lymphocyte, due to relative wide protein expressions that are related to the immune responses. Besides, cryo-leukocytes coupled with aICAM-1 functionalized liposome exhibited obvious anchoring effect in inflammation sites by the interaction of ICAM-1 antibody and ICAM-1 molecules that were over-expressed on inflammatory pulmonary endothelial cells, thus served as superior drug lung-targeting vehicle to maximally enhance the accumulation of traditional Chinese and Western medicines in the lungs. 68.1% of drug signals could be observed in lung tissues compared with other major organs after intravenous injection, significantly higher than that of micro-sized drug-loaded cryo-leukocyte (18.6%) and nano-sized drug-loaded aICAM-1-liposome (12.2%). In a lipopolysaccharide-induced acute pneumonia mice model, the drug-loaded cryo-leukocyte achieved superior anti-inflammation efficacy with 87.5% survival of mice after treatment.
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DOI
高影响因子
Regenerative Biomaterials
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2026
·
IF 8.7
Deng Peishen,Zheng Manhong,Du Bing,Liu Changyu,Cheng Renyi,Liu Chaofeng,Wang Fang,Dong Hangyu,Shan Yan,Xu Yanhua
Repairing large-scale craniomaxillofacial bone defects is hindered by a limited availability of stem-cell sources and a low osteogenic efficiency. To address these challenges, Fe3O4 nanoparticles were modified with methacrylic anhydride (MAA) which helped to introduce photopolymerizable methacryloyl groups, resulting in MAA-Fe3O4 nanoparticles that exhibit excellent magnetic properties and colloidal stability. These nanoparticles were incorporated into gelatin methacryloyl (GelMA) and covalently crosslinked to form an injectable, photocurable GelMA-Fe3O4 magnetic composite hydrogel. This hydrogel provides a three-dimensional (3D) culture microenvironment for human dental follicle stem cells (hDFSCs), and upon encapsulation, osteogenesis was significantly enhanced under a 100 mT static magnetic field (SMF). In vitro, GelMA-Fe3O4 hydrogels demonstrated increased porosity and improved mechanical properties, thereby significantly promoting hDFSCs proliferation, adhesion, and spreading. Additionally, under SMF exposure, the expression of osteogenesis-related genes and proteins, including ALP, Runx2, Col-I, and OPN, was significantly upregulated. In a rat calvarial defect model, bone mineralization centers with multi-site distribution were observed in the GelMA-Fe3O4 + SMF group as early as 4 weeks postoperatively, leading to high-quality defect repair. The limitations of traditional 'peripheral-to-center’ unidirectional repair were overcome by this model of synchronous multi-site osteogenesis, maximizing bone regeneration with a minimal number of stem cells and providing an efficient, controllable tissue-engineering strategy for the clinical treatment of craniomaxillofacial bone defects.
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高影响因子
Regenerative Biomaterials
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2026
·
IF 8.7
Zhang Yiqing,Huang Qilin,Meng Hao,Chen Junli,Hao Yaying,Liu Xi,Xu Yangmengyuan,Ma Liqian,Xu Zhan,Ma Kui,Hu Wenzhi,Pan Xiaohua,Fu Xiaobing,Zhang Cuiping
Diabetic wounds exhibit impaired healing owing to multiple pathological factors and current treatment options remain limited, underscoring the urgent need for novel therapeutic strategies. Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) are emerging as promising candidates for wound healing. However, the process from isolation to application of EVs is complex and time-consuming, which probably influences the activity and the therapeutic effects of EVs. To address these issues, we created the integrated platform for EV separation and controlled release. Gelatin microspheres (GMS) were prepared and functionalized with the variable domain of heavy-chain-only antibodies (GMS-VHH). GMS-VHH enabled the efficient capture of CD9-positive EVs in the culture supernatant of MSCs, forming EVs-loaded gelatin microspheres (GMS@EVs). The characterization of EVs demonstrated that the GMS-VHH isolation method outperformed traditional ultracentrifugation in terms of EV structural integrity. GMS@EVs exhibited excellent biocompatibility and promoted the proliferation of epidermal keratinocytes in vitro. In addition, the controlled release and the therapeutic efficacy of GMS@EVs were also observed in the treatment of diabetic wounds. Transcriptomic analysis illustrated that the wound healing-related genes were significantly upregulated and the pro-inflammatory genes as well as the wound healing-impaired genes were downregulated. Collectively, this study introduces a “ready-to-use” platform for EV isolation and controlled release, providing a promising strategy for diabetic wound repair.
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高影响因子
Regenerative Biomaterials
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2026
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IF 8.7
Meng Xiangbo,Chang Le,Wang Huajun,Li Jiming,Huang Cuishan,Jiang Yuxi,Zhang Yuting,Cao Huijuan,Li Ling,Yang Wenyao,Xu Jiake,Qin Ling,Zheng Xiaofei,Cheng Wenxiang,Wang Xinluan
The regeneration of osteochondral defect in osteoarthritis (OA-OCD) remains a significant clinical challenge, frequently accompanied by synovial inflammation and persistent joint pain. Tissue engineering scaffolds show promising application prospects; however, due to their lack of bioactive molecules, they often fail to effectively regulate extracellular matrix remodeling and inflammatory responses, resulting in poor repair outcomes. In our previous study, we identified epimedin C (Epi C) as one of the most bioactive compounds from Xianlinggubao (XLGB) through 3D human osteoarthritic chondrocyte pellet cultures, demonstrating significant anti-inflammatory and anabolic effects. In this study, we developed a novel biphasic scaffold incorporating Epi C to enhance osteochondral regeneration in an OA-like microenvironment. The biphasic scaffold consists of a cartilage phase [PLGA (50:50)] and a subchondral bone phase [PLGA (75:25)/β-TCP], both with biomimetic pore sizes, porosity and mechanical properties, while the incorporation of Epi C did not compromise the scaffold’s structural integrity. In vitro degradation experiments revealed that the cartilage phase degraded rapidly, facilitating the prompt release of Epi C, while the subchondral bone phase exhibited slower degradation, allowing for sustained drug release over an extended duration. In a rat OA-OCD model, PTP@Epi C scaffold significantly promoted subchondral bone regeneration compared to the OA-OCD group and PTP group. Additionally, the PTP@Epi C scaffold effectively inhibited the expression of TNF-α in the synovial tissue, thereby alleviating synovitis. Transcriptomic analysis and in vitro experiments demonstrate that Epi C downregulates NLRP3 mRNA expression by inhibiting the phosphorylation of IκBα and p65, thereby preventing p65 nuclear translocation and reducing NF-κB transcriptional activity. This ultimately suppresses NLRP3 mRNA levels and decreases downstream IL-1β secretion. In summary, the PTP@Epi C scaffold significantly enhances the osteochondral regeneration and improves the synovial microenvironment through a layered controlled release of Epi C, providing a novel approach for the OA-OCD treatment.
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PubMed
高影响因子
Regenerative Biomaterials
·
2026
·
IF 8.7
Du Yunzheng,Liu Zhuang,Chai Yamin,Wang Biao,Wang Lichun,Wang Zimeng,Guo Xiaofang,Zhou Guanlun,Yang Jingxuan,Zhu Chunling,Yang Leilei,Lv Xinyao,Ou Lailiang
Hemoperfusion has emerged as a crucial treatment for liver failure in clinics. However, the challenge remains in the incapacity to effectively and concurrently eliminate multiple harmful toxins, including inflammatory cytokines and bilirubin. This study employed molecular docking for directing the design of adsorbents. To develop a multi-target adsorbent material for liver failure, capable of absorbing both bilirubin and TNF-α, a functional platform was constructed. This platform employs a TiO2-modified polystyrene-based microsphere PSVT, which was designed for bilirubin adsorption, and coated with polydopamine and grafted with EGCG for TNF-α adsorption. The results confirm that EGCG possesses a high binding affinity for TNF-α, and the introduction of EGCG as a functional moiety markedly improves the clearance effectiveness of TNF-α. Meanwhile, PSVT served as the foundation to guarantee the effective removal of bilirubin. PSVT/P/EGCG exhibits outstanding clearance performance, with a maximum adsorption capacity of 263 ng/g for TNF-α and 23.91 mg/g for bilirubin, demonstrating its high efficiency in removing both inflammatory mediators and toxins. EGCG contributes exceptional antioxidant capabilities to PSVT/P/EGCG. The adsorbent exhibits remarkable biocompatibility and stability. The multi-target adsorbent material developed in this study has an extensive spectrum of potential applications in liver failure treatment employing an artificial liver.
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PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Yu Pengcheng,Lu Weiqi,Sun Huaxin,Huang Chengchen,Zhou Xiaolin,Wang Yuxing,Zhang Zhen,Fu Guosheng,Liu Hanxiong,Ren Kefeng,Sheng Xia
Postoperative atrial fibrillation (POAF) is the most prevalent form of secondary atrial fibrillation and increase the risk of adverse cardiovascular outcomes, such as stroke, heart failure, and increased mortality. Herein, we designed an andrographolide (Andr)-loaded degradable polymer patch to deliver the drug directly to the atrial tissue for prevention of POAF. The sterile pericarditis (SP) rat model was adopted for highly relationship to clinical practice. The patch-released Andr effectively reduced the incidence of atrial fibrillation from 90% to 20%, and alleviated local atrial inflammation and oxidative stress in vivo, by using electrophysiological detection and histological analysis such as immunofluorescence, western blot, PCR and so on. In HL-1 cells, we found the use of Andr-loaded patch could strongly inhibited the cell death, reactive oxygen species (ROS) generation and mitochondrial injury caused by LPS. Meanwhile, the use of Andr-loaded patch could effectively inhibited macrophages polarize towards M1. Mechanistically, we verified that the regulation of the cytoplasm and mitochondria Ca2+ and ROS dynamic balance was quite important both in vivo and in vitro. Our strategy proved by regulating the inflammatory microenvironment, ROS balance and Ca2+ homeostasis, and the Andr-loaded atrial patch was effective for POAF in the SP rat model. The electrical signal of atrial stromal reentry in the case of this model was successfully mined, and the results of calcium channel were basically consistent with that of electrical signal channel. In addition, we have reported the infiltration and polarization of local inflammatory cells in the atrial of POAF at the tissue section level. Our study served as a new inspiration for the treatment of arrhythmic diseases and other ROS- and Ca2+- associated local illnesses.
研究领域:
心血管医学
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药理学
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生物医学工程
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Zhao Miaoran,Chang Shuhua,Wang Yunpeng,Cao Jun,Pu Yuji,He Bin,Pan Shengsheng
Injectable facial fillers such as Sculptra® stimulate collagen regeneration to fill wrinkles; however, the collagen regeneration is not satisfactory due to the slow emergence of filling effect. In this study, we designed a regenerative dermal filler to provide both immediate and long-lasting filling effects. A hydrogel matrix composed of crosslinked hyaluronic acid (HA) and collagen was engineered to encapsulate porous poly(L-lactide) (PLLA) microspheres and tranexamic acid (TXA). The hydrogel matrix was administered via intradermal injection to achieve wrinkle filling. TXA is released to exert skin-whitening effects, while the porous PLLA microspheres and their degradation product, lactic acid, continuously stimulate collagen regeneration over an extended period. Facial volume increased immediately following hydrogel injection. Large amounts of new Type I and Type III collagens are generated. The porous structure of PLLA microspheres facilitated the “penetrating growth” of collagen fibers, which effectively filled facial depressions and smoothed wrinkles. Overall, the HA/collagen composite hydrogel filler exhibited excellent aesthetic effects.
研究领域:
生物材料学
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医学美容
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组织工程学
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2025
·
IF 8.1
Liang Min-Hao,Zhou Lei,Li Juan,Liang Bin,Zhou Liang-Yun,Xue Feng-Feng,Jiang Li-Bo,Hong Wei
Postmenopausal osteoporosis (PMOP) is a predominant form of clinical osteoporosis. It has led to significant health and social burdens for older patients. Reestablishing the balance between osteogenic and osteoclastic is a crucial strategy for treating PMOP. Curcumin (Cur), a naturally derived polyphenolic substance, has gained recognition as a viable option for treating osteoporosis. Despite its potential, the clinical use of Cur is hindered by its limited bioavailability and the presence of side effects. Nanoparticles modified with aspartic acid octapeptide (ASP8) exhibit a strong affinity for bone tissue, facilitating targeted delivery. This study presents novel acid-responsive zeolite imidazolate framework-8 (ZIF) nanoparticles modified with ASP8 and loaded with Cur (Cur@ZIF@ASP8, CZA). Upon delivery by this nanoparticle drug delivery system, Cur can effectively regulate bone homeostasis, offering a potential therapeutic strategy for osteoporosis. This study demonstrated that CZA nanoparticles could successfully transport Cur to bone tissue without significant toxicity. Furthermore, nanoparticles promote bone formation and inhibit osteoclast activity. They also modify the expression of related genes and proteins, such as OCN, ALP, CTSK, and MMP9. Significant evaluations utilizing microcomputed tomography, Masson’s staining, hematoxylin and eosin staining, and immunofluorescence staining demonstrated that intravenous CZA administration in ovariectomized mice resulted in bone destruction while simultaneously reducing overall bone loss. In conclusion, CZA nanoparticles hold promise as a therapeutic option for osteoporosis.
研究领域:
医学
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材料科学
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药理学
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PubMed
高影响因子
Regenerative Biomaterials
·
2024
·
IF 5.6
Di Yuntao,Wang Lu,He Wei,Liu Shuyan,He Yuqi,Liao Jie,Zhang Ruihong,Yin Lan,Xu Zhiwei,Li Xiaoming
Polypropylene (PP) mesh is commonly used in abdominal wall repair due to its ability to reduce the risk of organ damage, infections and other complications. However, the PP mesh often leads to adhesion formation and does not promote functional tissue repair. In this study, we synthesized one kind of aldehyde Bletilla striata polysaccharide (BSPA) modified chitosan (CS) hydrogel based on Schiff base reaction. The hydrogel exhibited a porous network structure, a highly hydrophilic surface and good biocompatibility. We wrapped the PP mesh inside the hydrogel and evaluated the performance of the resulting composites in a bilateral 1 × 1.5 cm abdominal wall defect model in rats. The results of gross observation, histological staining and immunohistochemical staining demonstrated the positive impact of the CS hydrogel on anti-adhesion and wound healing effects. Notably, the addition of BSPA to the CS hydrogel further improved the performance of the composites in vivo, promoting wound healing by enhancing collagen deposition and capillary rearrangement. This study suggested that the BSPA-modified CS hydrogel significantly promoted the anti-adhesion, anti-inflammatory and pro-angiogenesis properties of PP meshes during the healing process. Overall, this work offers a novel approach to the design of abdominal wall repair patches.
研究领域:
生物材料学
·
组织工程学
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DOI
PubMed
高影响因子
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