Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 6
·735-748
·
IF 4.5
Meng Rongqian,Tang Jianke,Yang Hong,Guo Lijun,Song Yongbo,Li Qiaoling,Niu Yulan
In this study, a simple and effective method was proposed to improve the electrocatalytic ability of overoxidized poly(3,4-ethylenedioxythiophene)-overoxidized polypyrrole composite films modified on glassy carbon electrode for rutin and luteolin determination. The composite electrode was prepared by cyclic voltammetry copolymerization with LiClO 4 -water as the supporting electrolyte. The peak current of rutin and luteolin on the composite electrode gradually decreased or even disappeared with the increase in the positive potential limit. After incubation in NaOH-ethanol solution with a volume ratio of 1:1, the composite electrodes prepared at positive potential limit greater than 1.5 V exhibited enhanced differential pulse voltammetry peak currents, reduced charge transfer resistance, larger effective specific surface area and higher electron transfer rate constant. The composite electrode prepared in the potential range of 0–1.7 V showed optimal electrocatalytic performance. The X-ray photoelectron spectroscopy results indicated that the content of −SO 2 /−SO and −C=N− groups in the composite film increased significantly after incubation. Further, the Raman spectra and Fourier transform infrared spectra revealed that the thiophene ring structure changed from benzene-type to quinone-type, and the quinone-type pyrrole ring was formed. The electrocatalytic mechanism of the composite film was proposed based on the experimental results and further verified by Density Functional Theory calculation.
研究领域:
分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 28
·3584-3589
·
IF 2.378
Yanqiong Lai,Xia Teng,Yanli Zhang,Hongbin Wang,Pengfei Pang,Chun Yang,Colin J. Barrow,Wenrong Yang
In this study, we developed a simple, sensitive, low-cost and label-free method to detect rutin by using double-stranded DNA-templated copper nanoclusters (dsDNA-CuNCs) as a fluorescent probe. The dsDNA-template can facilitate the formation of highly fluorescent CuNCs with a maximum emission wavelength of 575 nm. In the dsDNA-CuNC fluorescent probe, rutin was intercalated into the structure of dsDNA via hydrogen bonding and electrostatic interactions. The fluorescence of dsDNA-CuNCs was quenched considerably due to the electron transfer from CuNCs to rutin. This decrease in the fluorescence intensity of dsDNA-CuNCs can be used to monitor the concentration of rutin from 0.6 to 400 μM with a detection limit of 0.12 μM (S/N = 3). Compared with the previous reports, this method does not require any complex DNA sequence design, fluorescent dye label and sophisticated experimental techniques. Moreover, the proposed sensing system could be applicable for the detection of rutin in pharmaceutical samples.
研究领域:
生物化学
·
分析化学
·
药物分析
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 11
·e15961
·
IF 2.19
Qianwen Ru,Sheng Geng,Chungang Chen,Guizhao Liang,Benguo Liu
Tartary buckwheat bran (TBB), which is rich in proteins, is the main waste of tartary buckwheat processing. To improve the value of TBB, the noncovalent and covalent Tartary buckwheat bran protein (TBBP)/rutin complexes were prepared, and their capacities to fabricate β-carotene nanoemulsions were compared. The results indicated that the covalent complex exhibited the highest emulsifying performance, and the corresponding nanoemulsion had the lowest droplet size and polymer dispersity index (PDI) and the highest absolute value of ζ-potential, which could remain stable for four weeks. The high–temperature–short–time treatment and freeze–thaw processing had little influence on this nanoemulsion. However, it was very sensitive to high salt concentrations. Compared with the nanoemulsion prepared by the noncovalent complex, it had higher protein and rutin concentrations at the oil-water interface, which explained the improved stability of β-carotene against high temperature and UV irradiation. Practical applications In this study, the fabrication and characterization of β-carotene nanoemulsion stabilized by the complexes of tartary buckwheat bran protein and rutin are introduced. It can provide a reference for the utilization of other low-value cereal proteins and prompt the development of nutraceutical delivery systems based on food-derived protein-polyphenol complexes.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Jing Leng,Ping Li,Ling Bai,Yingqiong Peng,Yongfang Yu,Limin Lu
A Pd nanoparticles-graphene oxide (PdNPs/GO) hybrid as a highly sensitive sensing platform for determination of rutin was successfully prepared by a facile one-pot redox synthesis approach. Formation of PdNPs and loading onto the GO nanosheets were accomplished simultaneously by a redox reaction between H2PdCl4 and GO, without use of any additional reductants. Scanning electron microscopy (SEM) results demonstrated that PdNPs with an average size of 60 nm were distributed on the surface of GO nanosheets. Compared with the GO modified electrode, the PdNPs/GO modified electrode showed a better electrocatalytic activity toward the oxidation of rutin with larger peak current. Then the PdNPs/GO hybrid can be used as an enhanced sensing platform for the electrochemical determination of rutin. Under the optimal conditions, the anodic peak current was linear to the concentration of rutin in the range from 0.005 to 6 μM with the detection limit of 0.001 μM (S/N = 3). More importantly, the developed sensor can be used for the determination of rutin in medicine tablets with satisfactory results.
研究领域:
材料科学
·
分析化学
·
药物分析
✓ 已验证
DOI
高影响因子
Journal of Functional Foods
·
2018
·
IF 3.47
Qiao Lin,Xiu-Ying Chen,Ji Zhang,Yong-Liang Yuan,Wen Zhao,Bo Wei
The mechanisms underlying beneficial actions of Rutin, a food derived-polyphenolic bioflavonoid, against myocardial ischemia-reperfusion (MI/R) injury were detected. We found that Rutin could significantly ameliorate infarct size and attenuate cardiac dysfunctionto in some extent. Additionally, Rutin could also improve variation of myocardial zymogram and up-regulate antioxidant systems in heart. The selective SIRT1 inhibitor, Ex-527, exacerbated MI/R injury as evidenced by further increased oxidative stress and infracted area in heart tissue. Importantly, Ex-527 abolished the cardioprotective effect of Rutin in MI/R rats. Docking analysis further demonstrated the vital role of SIRT1 on Rutin's cardioprotective effect. We found that activation of SIRT1/Nrf2 signaling induced by Rutin contributes to the reduced oxidative stress, along with decreased apoptosis of cardiomyocytes in heart tissue. Overall, our findings suggest that SIRT1/Nrf2 signaling pathway may be a potential therapeutic target for the treatment of oxidative stress and apoptosis related myocardial diseases.
研究领域:
心脏病学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
DRUG DELIVERY
·
2022
·
IF 6.819
Dan Chen,Yurong Liu,Peiwen Liu,Yang Zhou,Longguang Jiang,Cai Yuan,Mingdong Huang
Thrombosis occurs in both macrovasculature and microvasculature, causing various cardio-cerebral vascular diseases. The lack of effective and safe antithrombotic drugs leads to a public health crisis. Mounting evidence suggests that protein disulfide isomerase (PDI) plays a critical role in the initial stage of thrombus formation, motivating the research of the feasibility of PDI inhibitors as novel anti-thrombotics. Rutin, one of the most potent PDI inhibitors, was reported to suppress platelet aggregation and thrombosis in animal models, but further studies and clinical translation were restricted due to its low aqueous solubility and oral bioavailability. In this work, we fabricated rutin-loaded lipid-based nano-formulation (NanoR) and characterized their physical–chemical properties, release profiles, pharmacokinetic process, and pharmacodynamic function against thrombosis in macrovessels and microvessels. NanoR provided increased solubility and dissolution of rutin to achieve earlier Tmax and higher Cmax than the sodium salt of rutin (NaR) after oral gavage. Ex vivo studies demonstrated that NanoR significantly inhibited thrombin generation and clot formation in the plasma of mice. Importantly, such effect was reversed by exogenous recombinant PDI, demonstrating the specificity of the NanoR. In direct current-induced arterial thrombosis model and ferric chloride-induced microvascular thrombosis model, NanoR exhibited greatly enhanced antithrombotic activity compared with NaR. NanoR also showed good safety performance according to tail bleeding assay, global coagulation tests, and histological analysis. Overall, our current results indicated that NanoR offers a promising antithrombotic treatment with potential for clinical translation.
研究领域:
药剂学
·
药理学
·
纳米技术
·
心血管疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Italian Journal of Animal Science
·
2022
·
IF 2.552
Shun Chen,Huijuan Liu,Jiaqi Zhang,Binbin Zhou,Su Zhuang,Xiaofang He,Tian Wang,Chao Wang
This study investigated the effects of different doses of rutin on the growth performance, immunity, intestinal barrier, and antioxidant capacity of broilers. A total of two hundred and fifty-six 1-day-old male broilers were divided into 4 groups and fed basal diets supplemented with 0 (control group), 250, 500 and 1,000 mg rutin/kg, respectively, for 42 days. In the starter period (days 1–21) and whole trial period (days 1–42), broilers fed diets containing 500 mg rutin/kg had significantly (p < 0.05) higher average daily feed intake, body weight and average daily gain and better feed conversion ratio. Dietary 500 mg rutin/kg increased the villus height, villus height to crypt depth ratio and villus area, while reducing the B cell lymphoma 2 associated X mRNA expression (p < 0.05). Dietary 500 mg rutin/kg increased the level of IgA in serum and decreased serum tumour necrosis factor-α (TNF-α) content, and the nuclear factor kappa-B, interleukin-2 and TNF-α mRNA expression in jejunal mucosa (p < 0.05). Simultaneously, 500 and 1,000 mg rutin/kg significantly decreased serum diamine oxidase activity, D-lactic acid and lipopolysaccharide concentration, increased activities of total superoxide dismutase and total antioxidant capacity in jejunal mucosa (p < 0.05), and upregulated mRNA expressions of genes related to intestinal barrier and antioxidant capacity in jejunal mucosa (p < 0.05). These findings indicate that dietary rutin, especially at 500 mg/kg, improves broilers’ growth performance, intestinal barrier function, immunity, and antioxidant capability, which may be related to the nuclear factor erythroid-2-related factor 2 (Nrf2) pathway.HighlightsDietary rutin improved growth performance, jejunal morphology, and intestinal barrier function.Dietary rutin enhanced the immunity via inhibiting NF-κB, and improved antioxidant capacity via activating Nrf2/HO-1 pathway in broilers.The optimal dose of rutin was 500 mg/kg in broiler diet.
研究领域:
动物营养学
·
免疫学
·
肠道生理学
✓ 已验证
产品关联
DOI
高影响因子
NEUROTOXICOLOGY
·
2023
·
IF 3.4
Tong Zhang,Chunmei Zhang,Yuyou Luo,Shuping Liu,Siyuan Li,Lixia Li,Yuxin Ma,Jing Liu
The present study aimed to investigate the protective effect of rutin on the injury of spinal motor neuron in rats exposed to acrylamide (ACR) the underlying mechanism. Fifty male Sprague-Dawley rats, aged 7–8 weeks, were randomly divided into control group, ACR group (20 mg/kg), low dose(100 mg/kg), medium dose (200 mg/kg) and high dose(400 mg/kg) rutin groups, ten rats in each group. The rats were given intragastric administration for 21 days. Every week, a neurobehavioral test was conducted. Nissl staining was used to observe the morphological changes in motor neurons in the L4-L6 segment of the spinal cord . Immunohistochemistry was used to identify AChE and ChAT in the rat spinal cord. Western blot was used to identify the expression of AChE, ChAT, P-ERK, ERK, and Nrf2 proteins in the rat spinal cord. The commercial kits were used to detect the presence of SOD, GSH, and LDH in the rat spinal cord. At the start of the second week, the medium and high dosage rutin group's rats' gait scores significantly decreased as compared to those of the ACR group. When rutin dosage was increased, the Nissl staining revealed that Nissl bodies was staining intensified compared to the ACR group. Immunohistochemistry and Western blot analysis revealed that AChE and ChAT expression changed when rutin dose was raised, but P-ERK and Nrf2 expression steadily increased in the spinal cord of rats in the medium and high dose groups compared to the ACR group. In the spinal cord of rats in each dosage group compared to the ACR group, the findings of the oxidative stress indices demonstrated that the expression levels of SOD and GSH rose with the increase of rutin dose, while the expression of LDH reduced with the rise of rutin dose. Rutin has an anti-oxidative impact through up-regulating the expression of P-ERK and Nrf2 proteins in the ERK/Nrf2 pathway, which may be connected to its protective action on motor neurons in the spinal cord of rats exposed to ACR.
研究领域:
神经科学
·
药理学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Zhifen Deng,Yuting Xia,Lang Chen,Yi Zhao,Ruihong Wang,Guizhao Liang
β-lactoglobulin (β-LG) is an essential nutrient in milk, but it is the primary allergen causing dairy allergy in humans. Currently, researchers are focusing on using flavonoids to covalently modify β-LG for improving its functionality. However, the impact and underlying mechanisms of rutin covalent modification on the functional properties and allergenicity of β-LG remain unclear. Here, we aim to investigate the changes in allergenicity, digestive characteristics, and antioxidant properties of β-LG after covalent modification using a combination of spectroscopy, enzyme-linked immunosorbent assay (ELISA), simulated digestion, and antioxidant assays. The results indicate that rutin forms covalent bonds with the free amino group, sulfhydryl group, and tryptophan of β-LG, leading to alterations in the secondary structure of β-LG. Furthermore, the modified β-LG exhibits improved antioxidant capacity and decreased allergenicity, along with reduced resistance to pancreatin digestion in vitro . This study provides novel insights and strategies to expand the functional application of β-LG.
研究领域:
食品科学
·
过敏学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2023
·
IF 6.1
Haixia Che,Jie Yi,Xiaoting Zhao,Hong Yu,Xianyao Wang,Rui Zhang,Xin Li,Jia Fu,Qian Li
As a common plant-derived dietary flavonoid, rutin receives the widespread attention because of its good antioxidant bioactivities. Protein kinase Cα (PKCα) is a serine/threonine kinase that is involved in uncountable cellular processes, among which ferroptosis, a novel form of cell death, is triggered by lipid peroxidation and has been reported to associate with pulmonary arterial hypertension (PAH). But it is still not well appreciated how rutin inhibits ferroptosis in PAH and what function of PKCα plays in this process. In this study, we firstly observed whether rutin could prevent PAH by attenuating ferroptosis with PAH animal model and pulmonary artery smooth muscle cells (PASMCs) under hypoxia. Mitochondrial metabolomics and network pharmacology were employed to clarify the metabolic alteration and screen target proteins, results showed PKCα was a vital node in rutin regulating mitochondrial metabolism related to ferroptosis in PAH. Based on molecular docking and multispectral analysis, we found rutin could directly interact with PKCα through hydrogen bonds, which could induce static quenching, and then influence the secondary structure of PKCα. In conclusion, these findings mainly point to a novel mechanism that rutin protect PAH rat by modifying the structure, altering activity of PKCα, and then suppressing ferroptosis. This work was great helpful to reveal that the interaction behaviours between small molecules and bio-macromolecules are the critical point to develop the natural biological active ingredients and give an insight into the potential applications of flavonoid in health and disease.
研究领域:
药理学
·
生物化学
·
分子生物学
·
心血管疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL PHARMACOLOGY
·
2025
·
IF 5.6
Ke Wang,Xiaomin Xia,Nan Wang,Jie Liu,Xue Li
Macrophage extracellular traps (METs) are protein network structures released by macrophages during immune defense initiation and inflammation regulation. Evidence shows that these protein structures can help kill pathogens. However, overproduction of METs can harm organisms. Therefore, novel strategies to inhibit the excessive production of METs during immune reactions need to be developed. This study introduces a novel type of anti-inflammatory and antibacterial nanocomposites (i.e., ZIF-8/Ce@rutin NPs) developed by doping the nanozyme Ce into the antibacterial zeolitic imidazole framework-8 (ZIF-8) and then loading the reactive oxygen species (ROS) scavenger rutin via electrostatic adsorption. Results showed that ZIF-8/Ce@rutin exerted bactericidal effects on gram-positive and -negative bacteria, thereby inhibiting MET production from the source. Furthermore, ZIF-8/Ce@rutin scavenged ROS and induced macrophage polarization into the anti-inflammatory M2 type, thereby inhibiting MET progression. This research shows that ZIF-8/Ce@rutin can restrain MET overproduction, providing a theoretical basis for using ZIF-8/Ce@rutin to treat inflammatory and infectious diseases.
研究领域:
纳米技术
·
免疫学
·
炎症研究
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2026
·
IF 12.4
Feiyu Jin,Weicheng Shen,Mingxin Ren,Yaohan Guo,Yuanye Yan,Ziyang Ye,Yuewei Peng,Bochen Yao,Kai Dong
Excessive oxidative stress and neuroinflammation caused by retinal detachment (RD) drive progressive photoreceptor degeneration, posing a major challenge to functional vision recovery even after successful anatomical reattachment. These limitations highlight the urgent need for alternative or adjunctive therapeutic strategies to protect photoreceptors. Here, smart supramolecular Cerium–Rutin nanoparticles (CRNPs) are developed for coordinated redox and immunomodulatory therapy in RD. CRNPs are constructed through the coordination assembly of cerium ions with the natural flavonoid Rutin, integrating the reversible Ce 3+ /Ce 4+ redox-switching capacity with the intrinsic anti-inflammatory activity of Rutin. This supramolecular nanozyme platform enables dynamic regulation of oxidative and inflammatory homeostasis within the injured retina. In vitro, CRNPs protect microglial and photoreceptor cells by scavenging reactive oxygen species (ROS) and suppressing inflammatory activation, demonstrating both anti-inflammatory and cytoprotective effects. In vivo, CRNPs effectively preserve photoreceptor morphology, protect outer nuclear layer integrity, enhance retinal electrophysiological responses and vision function in an RD model. Mechanistically, CRNPs first mitigate oxidative stress, which in turn suppresses activation of the NLRP3/ASC/caspase-1 signaling pathway, thereby reducing pro-inflammatory microglial activation and limiting the release of inflammatory mediators. Collectively, this study establishes a redox-active nanozyme platform capable of restoring retinal homeostasis and mitigating neuroinflammation, providing a promising nanotherapeutic approach for the treatment of RD and other oxidative stress–associated neurodegenerative diseases.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）