Science of Advanced Materials
·
2022
·Vol. 14
·期 2
·263-271
·
IF 1.067
Fan Jing,Wu Dan,Zhao Depeng,Hu Rui,Fu Zhihong,Li Xuemei
In this study, a novel small molecule estradiol guanidine derivative (Guanidoestradiol, GDT) for female reproductive system targeting and nitric oxide (NO) release has been constructed by conjugating 17β-estradiol and L-arginine through an esterification reaction. After
intraperitoneal administration in female mice, the serum Griess assay and immunohistochemical analysis of ovary and uterus were performed. GDT effectively increased NO concentration in the blood and produced some bio-effects that are beneficial to the reproductive system. The specific advantages
are as follows: (1) the introduction of guanidine groups and amino groups improves the water solubility and bioavailability of estradiol and is expected to exert other biofunctions; (2) the estradiol residue of GDT gives the NO donor the ability for ovarian and uterine targeting; (3) combined
administration of estradiol and NO to the uterus and ovary promoted endometrial thickening and expression of vascular proliferation markers; (4) the abundance of iNOS in tissues was reduced after treatment with NO donors. We suggest that Guanidoestradiol is a promising small-molecule drug
to enhance normal endometrial and ovarian function and may play a role in promoting reproduction.
研究领域:
药理学
·
生殖生物学
·
内分泌学
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2023
·Vol. 15
·期 2
·243-255
·
IF 0.9
Chen Aimin,Hu Ruihua
Schisandra polysaccharide (SCHP) was utilized as the target drug, and Zein (corn prolamine) was utilized as the drug carrier. First, SCHP/Zein nanoparticles (Zein-SCHP NPs) were prepared. Then, a polydopamine (PDP) coating was utilized to enhance the stability and tumor targeting of
Zein. Finally, the folic acid (FA)-targeting ligand was covalently attached to the PDP-modified Zein-SCHP@PDP NP surface to form a highly pH-sensitive NP (Zein-SCHP@PDP-FA NPs) with active/passive targeting. In the experiment, apart from physical characterization of Zein-SCHP-based nanomaterials,
the in vitro cytotoxicity and cell uptake properties of these materials were tested. In addition, an ovarian cancer cell line, SKOV3, was taken as the research object to explore the photodynamic-mediated killing effect of Zein-SCHP@PDP-FA NPs on this cancer cell. The results demonstrated
that the average particle size of Zein-SCHP@PDP-FA NPs was (379.68±1.36) nm, the zeta potential was (−43.68±1.67) mV, the encapsulation efficiency was (97.86±1.38)%, and the drug loading was (11.75±0.16)%. Transmission electron microscope (TEM) suggested that
the Zein-SCHP@PDP-FA NPs were spherical, and there was no adhesion between the particles. Differential scanning calorimetry (DSC) and far infrared (FIR) results indicated that SCHP existed in the NPs in the crystal structure and proved that the PDP and FA were successfully modified. The in
vitro release of the drug showed that Zein-SCHP@PDP-FA NPs had sustained release, and the CCK-8 test showed that Zein-SCHP@PDP-FA NPs were highly cytotoxic and could notably reduce the IC50 values of SCHP. Furthermore, in vitro uptake experiments revealed that Zein-SCHP@PDP-FA
NPs exhibited strong specific recognition in cells expressing different folate receptors. Compared with Zein-SCHP NPs and Zein-SCHP@PDP NPs, Zein-SCHP@PDP-FA NPs combined with photodynamic therapy enhanced reactive oxygen species (ROS) production in cells, downregulated hypoxia-inducible factor
(HIF)-1α and interleukin (IL)-6 proteins, and exerted more destructive effects on SKOV3 cells.
研究领域:
药剂学
·
肿瘤学
·
生物医学工程
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 5
·864-873
·
IF 1.474
Chen Ping,Yue Xinai,Dai Wei,Wang Xia,Li Yingying,Xie Hongjian,Jiang Dongmei,Jiang Yongmei
N-acetyl-L-cysteine-modified CdTe QDs (Cadmium Telluride Quantum Dots) were obtained by hydrothermal method, and DA (amino deoxyglucose), PEG (polyethylene glycol), and 9-p-D (9 polyamine acid) were obtained by ligand substitution, which were used for remodifying CdTe
QDs. Successively, a novel CdTe QDs nanoma-terial, CdTe-NALC, was obtained. This composite nanometer material was characterized by ultraviolet, fluorescence spectrum, nuclear magnetic resonance hydrogen-spectrum, and other methods to study further its biocompatibility and toxic effect on the
primary cultured mouse hippocampus neurons. PC12 cells were vitro-cultured and dissolved in the CdTe-NALC solution with different concentrations. After 24 h, the expression levels of proteins related to the cAMP (cyclic adenosine monophosphate)-CREB (cAMP response element-binding proteins)-BDNF
(brain-derived neurotrophic factor) signaling pathway in the cells were detected by ELISA (enzyme-linked immunosorbent assay) kit. In the experiment, the DA/9-p-D/PEG-modified CdTe QDs composite nanometer material was successfully manufactured by ligand replacement. The nanoparticles
had good dis-persibility with an average particle size of about 9.2 nm. DA/9-p-D/PEG modification improved the biocompatibil-ity of the QDs. CdTe composite nanomaterials could significantly reduce the cell activity of mouse hippocampal neurons and promote their apoptosis, with an evident
dose-apoptosis relationship. With the increase of CDTE-NALC solution concentration, cAMP, pCREB (phosphorylated CREB), and BDNF protein content decreased (P < 0.05).
研究领域:
纳米技术
·
神经科学
·
细胞生物学
·
生物化学
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2015
·Vol. 7
·期 11
·2380-2387
·
IF 2.598
Liu Yuangang,Sun Qingqing,Wang Shibin,Fan Jingqian,Chen Aizheng,Wu Wenguo
Normally a useful tissue engineering scaffold should provide suitable micropores and fibrous structure to mimic the natural extracellular matrix (ECM), which can induce desired cellular activities and guide tissue regeneration. In this work, a microporous and micro/nanofibrous silk
fibroin-poly(lactic-co-glycolic acid) (SF-PLGA) scaffold prepared by thermal induced phase separation (TIPS) was described. Minitab statistical software was used to analyze the TIPS parameters effect on the pore size, porosity and fibre diameter. Results showed that the ECM-like architecture
could be achieved by TIPS with the mean porosity 81 ± 16% and the pore size among 0.0–66.7 μm. Besides, cell viability tests revealed that the cells could proliferate on the SF/PLGA scaffolds. These results reveal that the SF-PLGA scaffolds prepared by TIPS are suitable
for the future tissue engineering applications.
研究领域:
生物材料
·
组织工程
·
生物医学工程
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2023
·Vol. 15
·期 3
·319-329
·
IF 0.9
Hu Ruihua,Chen Aimin
In recent years, traditional Chinese medicine (TCM), represented by tanshinone (Tas) and ganoderan polysaccharides, has attracted the attention of many scientists due to its mild effect of inhibiting tumors. In this research, poly-gamma-glutamic acid (γ-PGA) was degraded
into small molecule γ-PGA fragments by high-temperature acidolysis, and L-phenylalanine ethylester (L-PAE) was combined with small molecule γ-PGA fragments to generate γ-PGA-LA by dehydration condensation. The material was mixed with fat-soluble Tas IIA
to form PL-Tas IIA nanomicelles (NMs). In addition to physical characterization, the in vitro biological activity of the material was detected to establish a tumor-bearing nude mouse model, which was inoculated with cervical cancer HeLa cells. The nude mouse models were grouped, and
the effect of NMs on the growth of transplanted tumors was observed by intraperitoneal injection. The results revealed that the nanoparticle size was approximately 139.6±3.8 nm, and it had a good EPR effect, which was conducive to passive targeted therapy of tumors. The polydispersity
coefficient and zeta potential were 0.138±0.005 and 33.6±1.6 mV, respectively. The NM was cocultured with the cells under various concentration conditions, and the cell survival rate was more than 85%. The tumor cell uptake performance of the NM was ideal, and the cell uptake
ratio reached 71.62% at 60 min, as determined by flow cytometry. An in vivo tumor test demonstrated that PL-Tas IIA had a favorite tumor inhibition effect. The tumor-bearing nude mouse model showed that the prepared NMs can inhibit tumor growth, induce angiogenesis of xenografts, and
further induce tumor cell apoptosis, further verifying that the prepared NMs can inhibit cervical cancer tumor growth.
研究领域:
药学
·
肿瘤学
·
生物技术
·
材料科学
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2019
·Vol. 11
·期 10
·1376-1382
·
IF 1.158
Wang Bingjie,Ma Chuanjiang,Guo Chunjing,Wang Kaili,Yuan Dehao,Cheng Haoran,Liu Mengna,Fang Lei,Fan Xinxin,Chen Daquan
Drug resistance, perplexing a lot of doctors and patients, was an inevitable challenge in the field of effective cancer therapy. Poor water solubility, inefficient aggregation at tumor site and killing normal cells were also the great obstacles, which gave rise to failure in the tumor
clinical treatment. In order to overcome these difficulties, modified targeting nano-micelles (MT-NMs) loading Curcumin (Cur) and Chrysin (Chr) were designed. In this work, dithiodipropionic acid (DA), a reduction-sensitive material, acted as a "messenger" linker connecting Chr and oligomeric
hyaluronic acid (oHA). Then, the amphiphilic polymer, oligomeric hyaluronic acid-dithiodipropionic acid-Chrysin (oHA-DA-Chr), was synthesized. What's more, combinational therapy is a better way to cripple cancer drug-resistant and reinforce the therapeutic efficacy. In this study, 1H-NMR
was used to authenticate the structure of oHA-DA-Chr. The amphiphilic oHA-DA-Chr could self-assemble into nano-micelles (NMs) in water. The NMs with small size (223.2±2.4 nm) could concentrate well at tumor sites by enhancing permeability and retention effect (EPR) and CD44 receptor
targeting property of oHA. The Cur could release well from NMs when disulfide bond (S–S) of DA was exposed to tumor environment containing high concentration of glutathione (GSH).
研究领域:
药学
·
纳米技术
·
癌症研究
·
生物医学工程
✓ 已验证
DOI
高影响因子
Science of Advanced Materials
·
2017
·
IF 1.671
Zhang Xiong,Li Yuebin,Cai Yaxuan,Zhang Yi,Tian Yiqun,Wang Lin,Gu Haoshuang,Chen Wei
Fluorescent dye-doped silica nanoparticles (FDSNPs) have exhibited great potential in applications including solid-state lighting, chemical sensing, and biological theranostics. However, the wide application of FDSNPs is still a big challenge due to the lack of efficient synthesis strategy.
Herein, red fluorescent hybrid silica nanocapsules (FHSNCPs) were assembled by a green, convenient, and benign method based on Pluronic®F127 micelles as a soft template. Morphologies, structures, sizes, and fluorescence properties of the as-prepared silica nanocapsules were
characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR) and fluorescence spectroscopy. The size of the silica nanocapsules was well controlled from 20.5 nm to 23.6 nm by varying the concentration of F127 and tetramethylorthosilicate
(TMOS). Further investigations reveal that these nanocapsules exhibit monodisperse size, stable aqueous dispersibility, and novel optical properties, which show great potential for fluorescence imaging and temperature monitoring in biological tissues.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
光学
✓ 已验证
DOI
高影响因子
Applied Sciences-Basel
·
2022
·Vol. 12
·期 9
·4771
·
IF 2.838
Zhuoqi Duan,Zaixin Xie,Bao Zhou,Xiaobo Yang,Heng-Yong Nie,Yongmao Hu
Heritage buildings and monuments are mostly made from natural stone, which undergoes irreversible decay under outdoor conditions. The main reason for the contamination, degradation, and cracking of natural stones is water and oil permeation. Hence, modifications on stones rendering their surface self-cleaning are effective for stone protection. Reported in this paper is the development of a bionic approach to enabling self-cleaning stone surface via growing self-assembled polydopamine (PDA) as the adhesive layer on the stone surface, followed by depositing Al2O3nanoparticles derivatized by self-assembled monolayers of a fluorophosphonic acid (FPA). This approach ensured a robust surface modification that realized superhydrophobicity, as demonstrated on natural marbles, Hedishi, and Qingshi. The surface modification was thermally stable up to 400 °C.Keywords:self-cleaning stones;surface modification;polydopamine coatings;fluorophosphonic acid (FPA);self-assembled monolayers (SAMs);SAM-derivatized alumina particles;micro- and nanostructures
研究领域:
材料科学
·
表面化学
·
纳米技术
·
文化遗产保护
✓ 已验证
产品关联
DOI
高影响因子
NEUROGASTROENTEROLOGY AND MOTILITY
·
2022
·Vol. 35
·期 2
·e14488
·
IF 3.96
Mengjuan Lin,Guiying Hu,Baoping Yu
Background Hydrogen sulfide (H 2 S), an important endogenous gasotransmitter, is involved in the modulation of gastrointestinal motility, but whether it mediates the intestinal dysmotility in irritable bowel syndrome (IBS) is not known. This study explored the significance of cystathionine-β-synthase (CBS)/H 2 S signaling in stress-induced colonic dysmotility. Methods A rat model of IBS was established using chronic water avoidance stress (WAS). Colonic pathological alterations were detected histologically. Intestinal motility was determined by intestinal transit time (ITT) and fecal water content (FWC). Visceral sensitivity was assessed using the visceromotor response (VMR) to colorectal distension (CRD). Real-time PCR, Western blotting, and immunostaining were performed to identify the expression of CBS in the colon. The contractions of distal colon were studied in an organ bath system and H 2 S content was measured by ELISA. The effects of SAM, a selective CBS activator, on colonic dysmotility were examined. MEK1 was tested as a potential upstream effector of CBS/H 2 S loss. Key Results After 10 days of WAS, the ITT was decreased and FWC was increased, and the VMR magnitude in response to CRD was enhanced. The colonic CBS expression and H 2 S levels were significantly declined in WAS-exposed rats, and the density of CBS-positive enteric neurons in the myenteric plexus in WAS-treated rats was lower than that in controls. SAM treatment relieved WAS-induced colonic hypermotility via increased H 2 S production. AZD6244, a selective inhibitor of MEK1, partially reversed CBS downregulation and colonic hypermotility in WAS-treated rats. Conclusions & Inferences Decreased CBS/H 2 S signaling through increased MEK1 signaling might be important in the pathogenesis of chronic stress-induced colonic hypermotility. SAM could be administered for disorders associated with intestinal hypermotility.
研究领域:
胃肠病学
·
神经胃肠病学
·
药理学
·
应激生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Energy
·
2021
·
IF 17.881
Cheng Du,Chenglong Qiu,Zhongying Fang,Ping Li,Yijing Gao,Jianguo Wang,Wei Chen
Electrocatalytic nitrogen reduction reaction (NRR), which can produce ammonia from N 2 and H 2 O under ambient conditions, has emerged as a promising sustainable alternative to the Haber-Bosch (H-B) process. However, their unsatisfied conversion efficiency and selectivity severely restrict the real utilization of NRR, owing to the stubborn triple bond in the N 2 molecule and the competitive hydrogen evolution reaction (HER). Here, inspired from the local microenvironment of the nitrogenase, we report for the first time a facile and general strategy to boost the NRR selectivity and activity through the self-assembled monolayer (SAM) of hexanethiol (HEX) on a series of metal electrocatalysts (Cu, Au, Pt, Pd and Ni). Molecular dynamics (MD) simulations suggest that the HEX SAM provides a hydrophobic microenvironment to impede the diffusion and adsorption of water molecules and promote that of N 2 molecules, thus inhibiting HER and simultaneously improving the NRR performance. Notably, among all the prepared samples, the highest Faradic efficiency (FE) of 50.5% is achieved on Cu-HEX with NH 3 formation rate (R) of 1.2 μg h −1 cm −2 . Remarkably, for the HER-favored Pt catalyst, the highest R of 26.4 μg h −1 cm −2 is also achieved on Pt-HEX with FE of 1.8% under 1 cm 2 of electrode area. The present strategy not only represents a general diffusion-controlled method to engineer high-performance NRR electrocatalysts, but also provides a new insight into the effect of surface chemistry of catalysts on the NRR process and kinetics.
研究领域:
电化学
·
催化
·
材料科学
·
化学工程
·
可持续化学
✓ 已验证
产品关联
DOI
高影响因子
Science China-Life Sciences
·
2025
·
IF 9.5
Han Ziyi,Fu Jian,Gong Aiyan,Ren Wenkai
The gut microbiota plays key roles in host health by shaping the host immune responses through their metabolites, like indole derivatives from tryptophan. However, the direct role of these indole derivatives in macrophage fate decision and the underlying mechanism remains unknown. Here, we found that bacterial indole-3-propionic acid (IPA) downregulates interleukin-1beta (IL-1β) production in M1 macrophages through inhibition of nuclear factor-kappa B (NF-κB) signaling. Mechanistically, IPA binds specifically with methionine adenosyl-transferase 2A (MAT2A) to promote S-adenosylmethionine (SAM) synthesis, which facilitates the DNA methylation of ubiquitin-specific peptidase 16 (USP16, a deubiquitinase), and in turn promotes Toll-like receptor 4 (TLR4) ubiquitination and NF-κB inhibition. Furthermore, IPA administration attenuates sepsis in mouse models induced by lipopolysaccharides (LPS), showcasing its potential as a microbial-derived adjunct in alleviating inflammation. Collectively, our findings reveal a newly found microbial metabolite-immune system regulatory pathway mediated by IPA.
研究领域:
免疫学
·
微生物学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2020
·
IF 3.644
Hou Feifei,Wan Yupeng,Gan Qi,Xian Mo,Huang Wei
8-Azaguanine (1) is a special 1,2,3-triazole containing natural product that possesses potent antibacterial and antitumor activities. In the present study, the entire 8-azaguanine biosynthetic gene cluster was located from Streptomyces CGMCC4.1633. Targeted gene disruption, heterologous expression analysis, and feeding experiments identified crucial genes for 8-azaguanine production. Moreover, we characterized the structure of two novel metabolites, analyzed NO (or reactive nitrogen species) related genes 8-azgA/B and radical SAM enzyme homologous 8-AzgG, and verified the non-enzymatic ring formation reaction of 8-azaguanine 1,2,3-triazole. All of the data and presumptions provide insight into the timing and mechanism of the enzymatic and non-enzymatic pathway that produce 8-azaguanine-type 1,2,3-triazole.
研究领域:
微生物学
·
生物化学
·
遗传学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）