FOOD CONTROL
·
2023
·
IF 6
Jinxiang Wei,Yifei He,Zhiyi Song,Imran Mahmood Khan,Zhouping Wang,Caiyun Jiang,Xiaoyuan Ma
Herein, a surface-enhanced Raman scattering (SERS) and fluorescence dual-signal aptasensor has been developed for aflatoxin B 1 (AFB 1 ) detection. Fluorescence signal source CdTe quantum dots were decorated on the outer of silica nanoparticles and further functionalized with AFB 1 aptamer. Raman reporter molecules DTNB were decorated on the surface of gold nanoparticles (AuNPs) together with the aptamer complementary chains. The two parts of nanomaterials were combined by the base complementary pairing principle, to form satellite nanostructures with high SERS and low fluorescence signals following LSPR and FRET effect. The addition of AFB 1 led to the shedding of AuNPs due to the higher sensitivity of the aptamer towards the target and resulting in recovered fluorescence and decreased SERS signals. The developed method showed a linear relationship in the range of 10 −4 -10 3 ng/mL, with detection limit of 0.094 pg/mL. This technology had strong application prospects due to simplicity of operation and high accuracy.
研究领域:
分析化学
·
食品安全
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Siqingaowa Han,Chen Zhang,Shuang Lin,Xuanyu Sha,Wuliji Hasi
Recently, the phenomenon of fentanyls overdose leading to death is emerging in an endless stream. There is an urgent requirement to quickly identify fentanyl content in body fluids for medical and judicial purposes. With this in mind, we present a paper-based SERS substrate decorated with uniform gold nanospheres treated by chloride ion for the detection of fentanyl citrate in urine and serum. In particular, the paper-based SERS sensor was prepared by liquid/liquid self-assembly technique and chloride ion was introduced to clean and modify the substrate surface, which improved the sensitivity of the solid substrate with an enhancement factor (EF) as high as 1.64 × 10 5 . Moreover, the uniformity of each paper-based substrate and the repeatability on different batches of substrate were excellent, and there was no obvious change in the intensity response of Raman spectra within a month. As a result, the quantitative analysis of fentanyl citrate in artificial urine and rat serum were performed based on the modified paper-based substrate with the limit of detection as low as 0.59 μg/mL and 2.78 μg/mL, respectively. Both the concentrations of the two biological samples with the Raman signal intensity were linearly plotted and the recovery of the spiked samples with different concentrations was collected to verify the accuracy of the quantitative curves. All the results suggest that this work makes SERS method available for the rapid identification and quantitative analysis of illicit drug in the real biological samples.
研究领域:
分析化学
·
法医学
·
药物检测
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产品关联
DOI
PubMed
高影响因子
NANO
·
2018
·
IF 1.1
Chang Liu,Qianqian Su,Li Li,Jie Sun,Jian Dong,Weiping Qian
In this work, we designed a simple substrate-immersed solvothermal route for the one-step synthesis of novel ordered SiO 2 /Ag arrays, employing SiO 2 colloidal crystals as templates and alcohol as reducing agent. The Ag nanoparticles were uniformly deposited in situ onto SiO 2 colloidal crystals, which exhibited high surface enhanced Raman spectroscopy (SERS) activity and uniform SERS intensity. It was found that ordered SiO 2 /Ag arrays could rapidly scavenge the absorbed-Nile blue A (NBA) molecules from the surfaces with the assistance of H 2 O 2 , while the SERS signals of NBA decreased sharply and almost completely disappeared within four minutes. This can be attributed to the superior catalytic activity of Ag nanoparticles. After five times of re-immersion and re-absorbing process of NBA, the substrates could still keep ∼ 74.8% SERS intensity versus the original. The high activity and durability of the as-prepared SiO 2 /Ag SERS substrate endow them as a promising candidate for trace detection.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Han Zhang,Lin Sun,Yuan Zhang,Yan Kang,Huilian Hu,Huirong Tang,Yiping Du
This paper reports accurate synthesis of a new type of surface-enhanced Raman spectroscopy (SERS) substrate based on gold nanoparticles decorated 201 red silanized diatomaceous supports. The developed SERS substrate is easy to fabricate, cost effective and offers sensitive rapid detection. The performance and stability of the SERS substrate was investigated and the results showed good SERS activity of substrate that can last for more than 6 months. Tiazophos and phosmet pesticides in aqueous solutions can be detected clearly at a low concentration of 0.01 mg/L. The new substrate was applied to detect triazophos in apple pulp at 0.2 mg/kg. The results indicate that this substrate have good potential in rapid monitoring of pesticide residues in fruits and could be suitable for field-based applications, and routine laboratory analysis of chemicals.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 7
·2795-2804
·
IF 3.978
Cui-Fang Ning,Luying Wang,Ya-Fei Tian,Bin-Cheng Yin,Bang-Ce Ye
Exosomes are endogenous vesicles of cells, and can be used as important biomarkers for cancers. In this work, we developed a sensitive and reliable SERS sensor for simultaneous detection of multiple cancer-related exosomes. The SERS detection probes were made of bimetallic SERS-active nanotags, gold–silver–silver core–shell–shell nanotrepangs (GSSNTs), which were composed of bumpy surface nanorod (gold nanotrepang, GNT) cores and bilayer silver shells, and decorated with linker DNAs, which were complementary to the aptamer targeting exosomes. Three kinds of SERS detection probes were designed via the adoption of different Raman reporter molecules and linker DNAs. The capture probes were prepared by modifying specific aptamers of the target exosomes on magnetic beads (MBs). In the absence of target exosomes, SERS detection probes were coupled with MBs via specific DNA hybridization for use as aptamer-based SERS sensors. In the presence of target exosomes, the aptamer specifically recognized and captured the exosomes, and GSSNTs were subsequently released into the supernatant. Therefore, attenuated SERS signals were detected on the MBs, indicating the presence of target exosomes. The proposed aptamer-based SERS sensor is expected to be a facile and sensitive method for the multiplex detection of cancer biomarkers and has potential future applications in clinical diagnosis.
研究领域:
纳米技术
·
癌症生物学
·
分析化学
·
生物标志物检测
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产品关联
DOI
PubMed
高影响因子
OPTICAL AND QUANTUM ELECTRONICS
·
2020
·Vol. 52
·期 5
·1-11
·
IF 1.842
Wang Xiangxian,Wu Yuan,Wen Xiaolei,Zhu Jiankai,Bai Xuelin,Qi Yunping,Yang Hua
A composite structure of an Au nanodisk array and an Au film is designed to excite local surface plasmons and propagating surface plasmons and generate strong resonance coupling. The finite difference time domain method was used to study the surface plasmons effect of the structure. The composite substrate of an Au disk array with a 760-nm period and a 130-nm diameter was prepared via electron beam lithography. The SERS characteristics of the substrate were studied using rhodamine 6G (R6G) as a Raman probe molecule. The Raman intensity of R6G on the substrate reached values as high as 82.9% of the first measurement intensity, after the SERS substrate had been exposed to the atmosphere for 30 days. The results show that the proposed substrate yields good uniformity, reproducibility, and stability, thereby providing an effective and feasible scheme for the detection of molecules.
研究领域:
材料科学
·
光学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
OPTICS EXPRESS
·
2022
·Vol. 30
·期 21
·37795-37814
·
IF 3.833
Chuansong Chen,Chuansong Chen,Wenjie Zhang,Wenjie Zhang,Pengyi Duan,Wenying Liu,Muhammad Shafi,Muhammad Shafi,Xiaoxuan Hu,Can Zhang,Chao Zhang,Baoyuan Man,Baoyuan Man,Mei Liu
Improving the photo-induced charge transfer (PICT) efficiency by adjusting the energy levels difference between adsorbed probe molecules and substrate materials is a key factor for boosting the surface enhanced Raman scattering (SERS) based on the chemical mechanism (CM). Herein, a new route to improve the SERS activity of two-dimensional (2D) selenium and tin compounds (SnSex, 1 ≤ x ≤ 2) by the hybrid phase materials is researched. The physical properties and the energy band structure of SnSex were analyzed. The enhanced SERS activity of 2D SnSex can be attribute to the coupling of the PICT resonance caused by the defect energy levels induced by Se vacancy and the molecular resonance Raman scattering (RRS). This established a relationship between the physical properties and SERS activity of 2D layered materials. The resonance probe molecule, rhodamine (R6G), which is used to detect the SERS performance of SnSex nanosheets. The enhancement factor (EF) of R6G on the optimized SnSe1.35 nanosheets can be as high as 2.6 × 106, with a detection limit of 10−10 M. The SERS result of the environmental pollution, thiram, shows that the SnSex nanosheets have a practical application in trace SERS detection, without the participation of metal particles. These results demonstrate that, through hybrid phase materials, the SERS sensitivity of 2D layered nanomaterials can be improved. It provides a kind of foreground non-metal SERS substrate in monitoring or detecting and provide a deep insight into the chemical SERS mechanism based on 2D layered materials.
研究领域:
材料科学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY OF MATERIALS
·
2020
·
IF 9.567
Ziyang Cao,Peng He,Tao Huang,Siwei Yang,Sancan Han,Xianying Wang,Guqiao Ding
Fully exploiting plasmonic coupling of nanostructured metals is an effective method to promote surface-enhanced Raman scattering (SERS) performance for trace detection of molecules. Herein, we propose a cross-section strategy to maximize plasmonic coupling of silver nanoparticles (AgNPs) in a graphene-based membrane. Specifically, AgNPs are isolated by water-dispersible graphene (W-Gr) and enriched in the vicinity of W-Gr edges when assembling into a macroscopic membrane, thus affording AgNPs on the cross section with uniform and proper gaps in the vertical plane to generate maximal plasmon coupling. Moreover, the superior sensitivity (5 × 10–13 M for R6G) to most reported graphene–metal structures and the long-term stability against aerobic oxidation jointly make the cross-section of the AgNPs/W-Gr membrane a potential SERS substrate for trace-molecule detection. The revealed mechanism for AgNPs enrichment near edges highlights the importance of the flow-directed assembly process of W-Gr. This work provides new insight into the interpretation and utilization of two-dimensional materials as building blocks in high-performance SERS sensing.
研究领域:
材料科学
·
光学
·
分析化学
✓ 已验证
DOI
高影响因子
Small
·
2015
·Vol. 11
·期 23
·2798-2806
·
IF 8.368
Lei Wu,Zhuyuan Wang,Kequan Fan,Shenfei Zong,Yiping Cui
A surface enhanced Raman scattering (SERS)-assisted 3D barcode chip has been developed for high-throughput biosensing. The 3D barcode is realized through joint 2D spatial encoding with the Raman spectroscopic encoding, which stores the SERS fingerprint information in the format of a 2D array. Here, the concept of SERS-assisted 3D barcode is demonstrated through multiplex immunoassay, where simultaneous detection of multiple targets in different samples has been achieved using a microfluidic platform. First, multiple proteins in different samples are spatially separated using a microfluidic patterned antibody barcode substrate, forming a 2D hybridization array. Then the SERS probes are used to identify and quantify the proteins. As different SERS probes are labeled with different Raman reporters, they could be employed as “SERS tags” to incorporate spectroscopic information into the 3D barcode. In this 3D barcode, the 2D spatial information helps to differentiate the samples and targets while the SERS information allows quantitative multiplex detection. It is found that the SERS-assisted 3D barcode chip can not only accomplish one-step multiplex detection within 30 min but also achieve an ultrasensitivity down to 10 fg mL −1 (≈70 aM), which is expected to provide a promising tool for high-throughput biomedical applications.
研究领域:
生物传感器
·
纳米技术
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Yaodi Zhu,Weijia Liu,Shijie Liu,Miaoyun Li,Lijun Zhao,Lina Xu,Na Wang,Gaiming Zhao,Qiuying Yu
In this study, we developed a novel and sensitive AgNPs self-assembled solid-phase (AgNPs-SASP) SERS substrate platform using seed-mediated and liquid interface self-assembly methods and applied the platform for the detection of three bacterial spores. Multivariate statistical analysis (HCA and LDA) were employed for identification and categorization of the obtained data. The results illustrated that AgNPs-SASP exhibited high reproducibility and Raman enhancement effect (1.43 × 10 4 ). The Raman shift bands of the three bacterial spores ranged from 400 to 1800 cm −1 demonstrating the difference in reigon and intensity. And the intensities of their Ca 2+ -DPA Raman bands are significantly different. HCA results revealed that the spectra for the three bacterial spores were statistically different while LDA completely differentiated the spectra for the three bacterial spores with 100 % sensitivity and specificity. Overall, the novel SERS platform based on AgNPs-SASP provides an effective tool for food safety risk control and detection.
研究领域:
食品科学
·
分析化学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2024
·
IF 3.6
Lulu Liu,Wenrui Ma,Haolan Tan,Chuang Ge,Bei Zhang,Chunyan Wang,Yang Zhang,Yi Xu
The in situ monitoring of quorum sensing (QS) signal molecules in bacterial biofilms is crucial for the diagnosis and treatment of bacterial biofilms. In this work, a surface-enhanced Raman scattering (SERS) chip integrated with functional units of bacterial biofilms and in situ SERS detection is proposed for the monitoring of pyocyanine, an important signal molecule and biomarker secreted by Pseudomonas aeruginosa. The SERS chip was composed of a top self-assembled nano-silver SERS substrate layer, a PDMS layer containing a micro-chamber array and bottom quartz layer. The nano-silver SERS substrate showed good homogeneity and stability with an enhancement factor of up to 1.84 × 108 for pyocyanine, and the design of the SERS substrate located at the top layer of the chip could effectively attenuate interference from complex matrices. Using the SERS chip, a detection limit of as low as 1 nM for pyocyanine with a wide detection range of 1 nM–100 μM was observed. The proposed SERS chip could achieve the monitoring of pyocyanine during the growth of Pseudomonas aeruginosa biofilms with and without the treatment of different types of antibacterial drugs. Furthermore, pyocyanine secreted by Pseudomonas aeruginosa was confirmed using mass spectrometry (MS), and the concentration relationship obtained via MS/MS was consistent with that obtained via SERS detection. The proposed method based on the SERS chip for monitoring QS in bacterial biofilms offers the advantages of in situ detection, flexibility and efficiency.
研究领域:
微生物学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Qiao-Yan Jiang,Ding Li,Yuhui Liu,Zheng-Sheng Mao,Youjia Yu,Ping Zhu,Qilan Xu,Yang Sun,Li Hu,Jie Wang,Jin Chen,Feng Chen,Yue Cao
A novel, recyclable, portable, and low-cost SERS sensor-AuBPs@MoS 2 @tinfoil box is fabricated layer-by-layer by self-assembly method for the first time, which achieved the purpose of high throughput SERS tracking. Diquat (DQ) is one of the most widely used herbicides in the world since paraquat was banned. Benefiting from the “hot spots” generated by AuBPs@MoS 2 , the sensor successfully realizes straightforward SERS recognition of DQ in complex compounds, which detection limit is as low as 1.0 × 10 −15 M. The preprocessing is not required for the detection of real samples, which promotes the practical application of forensic medicine and environmental engineering.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）