SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Hongbo Li,Mingbin Liu,Weihua Zhao,Jiamei Pu,Jianguo Xu,Suqin Wang,Ruqin Yu
Nucleic acids-sensing methods provide the potential to enable and hasten early disease detection and to rationalize treatments, especially for cancers. In this work, a colorimetric label-free sensing system based on target-triggered split-to-intact remodeling of dual-G-rich duplex probe (Dual-GDP) has been developed for amplified signaling of Pax-5 gene. In this sensing system, the Dual-GDP is splited into one hairpin-structured G-rich probe (HGP) and one duplex-shaped G-rich probe (DGP), which can be re-built to an intact form through target gene triggered accurate base-pairing. Significantly, this unique split-to-intact remodeling behavior allows the occurrence of a well-designed active isothermal amplification that can amplify target response with seven stages to multi-channel accumulate numbers of G-quadruplex transducers. When specifically combined with hemin, the formed G-quadruplex-hemin complexes, also known as DNAzymes, can strongly catalyze the oxidization of TMB to a colored TMB + and robustly reflect the target gene even in complex biological environments. We demonstrate that this method offers simplified operations and improved assay abilities for Pax5 gene analysis. We envision these findings might provide new insights to exploit versatile oligonucleotide probes and nucleic acid-based amplification strategies for diverse bioanalytical applications.
研究领域:
分子生物学
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分析化学
·
癌症诊断
·
核酸传感
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 18
·4063-4071
·
IF 3.286
Lin Shan,Zheng Danmin,Li Ailing,Chi Yuwu
It is well known that 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized into blue or yellow oxidzed TMB (oxTMB) with the catalysis of peroxidase or mimetic enzyme of platinum nanoparticles (Pt NPs). In this work, we found that TMB could be oxidized into very stable black oxTMB with the catalysis of Pt NPs under certain chromogenic reaction conditions. For the first time, the black oxTMB was revealed to consist of nanowires (oxTMB NWs) with lengths of more than 100 μm and diameters of around 100 nm. The black oxTMB NWs showed very strong light absorption ability, thus could be used to greatly amplify the signal of Pt NP-based immunochromatography test strips (ICTSs). The Pt NP-based ICTSs with black oxTMB NW signal amplification have shown much better assay ability (linear response range and limit of detection) than those of gold nanoparticle (Au NP)-based ICTS, Pt NP-based ICTS, and Pt NP-based ICTS with blue or yellow oxTMB signal amplifications. The developed Pt NP-oxTMB NW-based ICTS has been demonstrated to be a new, accurate, sensitive, selective, and rapid immunosensor for quantitative detection of antigens such as human chorionic gonadotropin (HCG).
研究领域:
分析化学
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纳米技术
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Meiling Tian,Wenyue Xie,Ting Zhang,Yang Liu,Zhisong Lu,Chang Ming Li,Yingshuai Liu
This work reports a low-cost, fast and highly sensitive lateral flow immunoassay (LFIA) platform with Prussian blue nanoparticle (PBNP) as a new reporter, which efficiently mediates an on-demand cascade signal amplification by catalyzing oxidation of chromogenic reagent 3,3′,5,5′-tetramethylbenzidine (TMB). In this case, PBNPs are accumulated on the test line through the specific immune interaction, resulting in a blue-colored line. The colorimetric signal can be further amplified via the peroxidase mimic PBNP-catalyzed TMB oxidation to produce an insoluble blue-violet precipitate. Under an optimal condition, a limit of detection (LOD) at 10 pg mL −1 is achieved for rabbit IgG (RIgG) detection, which is 2–3 orders of magnitude better than the conventional LFIA strip using colloidal gold nanoparticle as a label. A macromolecular RIgG and a small molecular mycotoxin ochratoxin A (OTA) are also successfully analyzed in mimicking samples, demonstrating its good practicability of the proposed platform in a variety of fields.
研究领域:
纳米技术
·
分析化学
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生物传感器
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免疫学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Peng Bo Niu,Yong Qiong Wang,Rong Hu,Tong Yang
With the improvement of the quality of life, the assessment of total antioxidant capacity (TAC) has attracted much attention in the field of a healthy diet, food analysis, or health guidelines. In this work, Cu nanoparticles (NPs)/CoO/carbon nanofibers (CNFs) as a promising nanozyme for peroxidase (POD) mimicking was synthesized by electrospinning and carbonation. Subsequently, a colorimetric sensing platform is constructed for TAC assessment by using 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogenic substrate based on Cu NPs/CoO/CNFs under presence of H2O2. The enhanced POD catalytic efficiency of Cu NPs/CoO/CNFs for TMB oxidation (ox-TMB) was about 3.2-fold higher than that of bare Cu NPs/CNFs or CoO/NFs, which was ascribed to the synergistic catalytic effect and the generation of heterogeneous interfaces between Cu NPs and CoO in CNFs. When some antioxidants such as AA, FA, TA, and GA were introduced in the chromogenic system, ox-TMB was reduced to colorless TMB, allowing the subsequent assessment of TAC. The results show that the limits of detection toward AA, FA, TA, and GA were 8.7, 8.3, 2.7, and 2.4 μM, respectively. Moreover, this proposed method was used for the TAC assay in real samples including fresh juices and commercial beverages, which showed good promise in practical applications such as food nutrition assessment, food analysis, personalized nutrition intervention, or staple food research.
研究领域:
材料科学
·
分析化学
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食品科学
·
生物技术
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Chenzhao Wang,Xiujuan Wang,Xinru Gu,Hongmiao Sun,Xinxi Chen,Guoying Sun,Zhibing Wang
Phosphatidylethanolamine (PE) is an important indicator for evaluating the nutritional value and quality of food. In this study, a multimodal detection method (UV/smartphone/fluorescence/paper-based sensing platform) was developed for PE detection in food on the basis of the peroxidase-like activity and fluorescence properties of cerium-doped carbon dots (Ce-CDs). The prepared CDs generated singlet oxygen ( 1 O 2 ) through the Russell mechanism, which oxidized 3,3′,5,5′-tetramethylbenzidine (TMB) to produce ox-TMB. The addition of PE changed the surface charge of Ce-CDs, enhancing their affinity with TMB and causing the solution to turn bluer. In addition, these CDs exhibited excellent optical properties and fluorescence stability. In fluorescence detection, PE significantly quenched the fluorescence of the reaction system through an inner filter effect mechanism. Furthermore, the designed paper-based sensing platform provided a novel tool for on-site high-throughput detection of PE. The signal values of Ce-CDs in various detection modes exhibit a good linear relationship with PE concentrations ranging from 0.1 to 1000 μg/mL, with a lowest detection limit of 0.023 μg/mL. More importantly, all methods successfully realized the detection of PE in real food samples and obtained satisfactory results. This study not only developed multiple detection methods based on the same material but also ensured the accuracy of the results through cross-validation between the different methods.
研究领域:
食品科学与技术
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分析化学
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纳米材料
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生物传感器
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荧光传感
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2018
·
IF 5.123
Yushen Liu,Juan Wang,Chao Zhao,Xiaoxiao Guo,Xiuling Song,Wei Zhao,Sijie Liu,Kun Xu,Juan Li
Listeria monocytogenes ( L. monocytogenes ) is one of the most common food-borne pathogens . The authors describe a sensitive and reliable multicolorimetric assay for L. monocytogenes using a sensing system based on TMB 2+ etching of gold nanorods . Apt-MNP was used as the capture probe, and IgY-BSA-MnO 2 NPs was chosen as an oxidase-like nano-artificial enzyme to oxidize TMB to generate TMB 2+ . Under the optimized conditions, the longitudinal shift of localized surface plasmon resonances had a linear correlation with the L. m onoc ytogenes concentration in the range between 10 to 10 6 cfu mL −1 . Meanwhile, the sensing system can generate vivid color responses as colorful as a rainbow, and the limit of detection is as low as 10 cfu mL −1 at a glance. Recoveries ranging from 97.4 to 101.3% are found when analyzing spiked food samples without pre-enrichment. In our perception, it shows promise in rapid instrumental and on-site visual detection of L. monocytogenes .
研究领域:
微生物学
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纳米技术
·
食品安全
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yifan Cui,Qiulan Li,Dezhi Yang,Yaling Yang
Peroxidase (POD)-mimicking nanozymes have got great progress in the sensing field, but most nanozyme assaying systems are built with a single-signal output mode, which is vulnerable to the effect of different factors. Thus, establishment of a dual-signal output mode is necessary for acquiring dependable and durable performance. This work described an Fe doped noradrenaline-based carbon dots and Prussian blue (Fe,NA-CDs/PB) nanocomposite as a POD-like nanozyme and modified gold nanoparticles (AuNPs) for the colorimetric and surface-enhanced Raman scattering (SERS) dual-mode sensor of Pb(II) in traditional Chinese medicine samples. With 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) and 3,3′,5,5′-tetramethylbenzidine (TMB) as the substrates, it was found that the addition of Pb(II) inhibited the POD-like activity of Fe,NA-CDs/PB and AuNPs, so it was used for colorimetric and SERS dual-mode assays. The POD-like activity was shown to be a “ping-pong” catalytic mechanism, whereas the addition of Pb(II) produced noncompetitive inhibition with modulatory effects on Fe,NA-CDs/PB. The linear response range for colorimetric and SERS sensor detection of Pb(II) was 0.01–1.00 mg/L with the detection limit of 5 μg/L and 8 μg/L, respectively. This dual-mode detection system shows excellent selectivity. More importantly, the Pb(II) in traditional Chinese medicine samples have successfully assayed with good recovery from 90.4 to 108.9 %.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
传统中药
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DOI
高影响因子
LUMINESCENCE
·
2020
·Vol. 35
·期 8
·1350-1359
·
IF 1.855
Liu Zhang,Yue Hou,Xiaoyan Guo,Wei Liu,Congcong Lv,Chunyang Zhang,Yan Jin,Baoxin Li
In this study, three types of Fe(III) bipyridyl or phenanthroline (Fe(III)-L 3 ) complex could directly catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to induce blue chromogenic changes without H 2 O 2 . Fe(III)-L 3 complex could induce a colour change in TMB directly after a short incubation time. Due to the high oxidase-like activity of the Fe(III)-L 3 complexes, superoxide anion radicals (O 2 •– ) were formed in solution. Intermediates radical involving oxo-iron species were then produced that oxidized TMB to its oxidation products (oxTMB), which had an absorbance maximum at 652 nm. Glutathione (GSH) could inhibit the oxidation reaction of the Fe(III)-L 3 complex–TMB system, a rapidly colorimetric method was established for the specific detection of GSH that had a detection limit of 0.1 μM. Furthermore, Fe(III)-L 3 complexes could catalyze TMB to oxTMB directly without H 2 O 2 . This fast and simple colorimetric method may open a new avenue for application in the point-of-care diagnosis field using the TMB chromogenic system.
研究领域:
分析化学
·
生物化学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 20
·4249-4256
·
IF 6.331
Mingmei Shen,Yuanyuan Wang,Xianwen Kan
Thrombin plays an essential role in blood coagulation and some physiological and pathological processes. The convenient, rapid, sensitive, and specific detection of thrombin is of great significance in clinical research and diagnosis. Herein, surface molecularly imprinted polymer (MIP) was modified on aptamer-functionalized Fe3O4 nanoparticles (MIP–aptamer–Fe3O4 NP) for thrombin colorimetric assay by taking advantage of the peroxidase-like activity of Fe3O4 NP. With the adsorption of thrombin into imprinted cavities, the exposed surface area of Fe3O4 NP decreased, causing a decrease in its peroxidase-like activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. On the other hand, the reductive amino acids on the thrombin surface also impeded the oxidation of TMB. Both phenomena caused the light blue color of the sensing solution. Thus, a specifically sensitive colorimetric approach for the visual detection of thrombin was proposed with a linear range and limit of detection of 108.1 pmol L−1–2.7 × 10−5 mol L−1 and 27.8 pmol L−1, respectively. Moreover, due to the double recognition elements of MIP and aptamer, the prepared MIP–aptamer–Fe3O4 NP showed higher selectivity to thrombin than that based on only one recognition element. It is worth noting that no special property (e.g. electrochemical or fluorescence activity) of the template was required in this work. Thus, more template molecules can be easily, selectively, and sensitively detected based on the proposed MIP–aptamer–mimic enzyme colorimetric sensing strategy.
研究领域:
生物化学
·
纳米技术
·
临床诊断
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2018
·Vol. 410
·期 20
·4805-4813
·
IF 3.307
Wang Yanying,Liu Yaqin,Ding Fang,Zhu Xiaoyan,Yang Li,Zou Ping,Rao Hanbing,Zhao Qingbiao,Wang Xianxiang
In this study, we developed a simple colorimetric approach to detect glutathione (GSH). The proposed approach is based on the ability of CuS-PDA-Au composite material to catalytically oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to ox-TMB to induce a blue color with an absorption peak centered at 652 nm. However, the introduction of GSH can result in a decrease in oxidized TMB; similarly, it can combine with Au nanoparticles (Au NPs) on the surface of CuS-PDA-Au composite material. Both approaches can result in a fading blue color and a reduction of the absorbance at 652 nm. Based on this above, we proposed a technique to detect GSH quantitatively and qualitatively through UV-Vis spectroscopy and naked eye, respectively. This approach demonstrates a low detection limit of 0.42 μM with a broad detection range of 5 × 10 −7 –1 × 10 −4 M with the assistance of UV-Vis spectroscopy. More importantly, this approach is convenient and rapid. This method was successfully applied to GSH detection in human serum and cell lines.
A colorimetric approach has been developed by exploiting the peroxidase-like activity of CuS-polydopamine-Au composite for sensitive glutathione detection.
研究领域:
分析化学
·
生物化学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Ying Zhang,Wang Ren,Hong Qun Luo,Nian Bing Li
A new magnetic bead-based cascade amplification strategy for highly sensitive visual detection of proteins (thrombin as a model analyte) was developed by coupling target-triggered hybridization chain reaction (HCR) with the synergistic catalysis of DNA concatemer-mediated hemin/G-quadruplex DNAzymes and Pt nanozymes. Initially, the biotinylated primer DNA (P-DNA) was complementary with aptamer to form dsDNA which was further linked to streptavidin-coated magnetic bead (MB), thereby fabricating the expected MB-based aptasensor . In the presence of target TB, the aptamer was taken away from the aptasensor, and the free P-DNA immediately triggered HCR to spontaneously form DNA concatemer-directed nanochains with numerous DNAzymes and Pt nanoclusters (PtNCs) to achieve cascades signal amplification . The dual peroxidase mimetics catalyzed the H 2 O 2 -mediated oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into the colored TMB oxides (oxTMB), causing intensified color change of the chromogenic solution for the highly sensitive naked-eye detection of as low as 100.0 pM TB. In this strategy, the employment of magnetic separation and exonuclease III (Exo III)-assisted digestion of residual dsDNA minimized the background noise and avoided the false positive results, greatly improving the detection accuracy and sensitivity with a low limit of detection (LOD=15.0 pM). The proposed visual platform has promise for detecting various types of proteins with careful DNA sequence designs.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 2
·1-7
·
IF 6.408
Zhang Bing,Wang Xue,Cheng Yan
A photochromic immunoassay was built for tumor marker detection based on ZnO/AgI nanophotocatalyst. Frist, ZnO/AgI nanoparticles were synthesized and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and Fourier transform infrared spectrometry (FTIR). The color development is caused by tetramethyl benzidine (TMB) solution oxidated by ZnO/AgI nanomaterials. The electron transitions in ZnO/AgI nanomaterials are driven by visible light irradiation, generating photogenerated hole and oxidizing TMB to blue solution. Appropriate band width between ZnO and AgI promotes separation of photogenerated electrons and holes and enhances oxidation efficiency. A sandwich-type immunoassay was constructed based on ZnO/AgI nanomaterial as labels. The absorbance at 650 nm of reaction solution is positively correlated with antigen concentration. The developed immunoassay showed good performance for carcinoma embryonic antigen (CEA) detection in the range 0.1–7.0 ng/mL with a detection limit of 65 pg/mL. The photochromic immunoassay also exhibited preferable selectivity, repeatability, and stability. Graphical abstract A novel colorimetric immunoassay was constructed based on ZnO/AgI photocatalyst. ZnO/AgI nanomaterials occur electron transitions under visible light irradiation and generate photogenerated hole, which can oxidize TMB to blue solution. Carcinoembryonic antigen in sample was detected sensitively due to the high catalytic efficiency of ZnO/AgI nanomaterials.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）