Arabian Journal of Chemistry
·
2015
·
IF 3.725
Vikramjeet Singh,Amita Nand,Sarita,Jiwen Zhang,Jingsong Zhu
Comparative study for non-specific adsorption of cell lysate and serum on recently developed biosensor surfaces is reported. Different surface chemistries including, polyethylene glycol (PEG), α-cyclodextrin (CD), hydrogel dextran and surface initiated polymerization (SIP) based gold surfaces were taken into account for this study. Various techniques including, surface plasmon resonance imaging (SPRi) and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry (MALDI−TOF/TOF MS) technique to evaluate the surfaces with Fourier transform infrared spectroscopy (FTIR) for the confirmation of surface fabrication were used. A high non-specific adsorption response of cell lysate and serum was observed on these so-called non-fouling surfaces. The obtained results from this comparative study provided some hope to explore SIP and dextran surface as a universal platform for biosensor applications. SIP produced best result and showed high sensitivity and minimum non-specific adsorption. We believe that this comparative study will surely help the researchers to further upgrade these surfaces and especially SIP to make it more universal for biosensor microarray applications including biomarker discovery in high throughput format.
研究领域:
生物技术
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 20
·4862
·
IF 4.2
Li Song,Yan Liang,Yilin Li,Tingting Guo,Hui Li,Shuxuan Liang
With the advantages of a high specificity, a long half-life, and a high safety, the use of antibody biologic drugs, including camrelizumab, has been rapidly increasing in clinical practice. Camrelizumab, an immune checkpoint inhibitor and humanized monoclonal antibody, is used to treat several advanced solid cancers. Measuring its concentration supports personalized dosage adjustments, influences treatment decisions for patients, strengthens the control of disease activity through therapeutic drug monitoring, and helps evaluate and prevent drug interactions in combination therapy. Because antibodies are present in complex biological matrices, quantifying monoclonal antibody drugs is challenging, and must rely on precise, selective, and reliable analytical methods. In this study, a quadrupole time-of-flight mass spectrometry TripleTOF 6600+ (AB SCIEX, Framingham, MA, USA) system equipped with a Turbo V ion source was used for the qualitative analysis of monoclonal antibodies using the data-dependent acquisition (IDA) MS/MS mode, followed by quantitative analysis using a targeted MRMHR workflow. This method showed a good linear relationship within the range of 4–160 μg/mL, with a correlation coefficient of R2≥ 0.996. It demonstrated an acceptable accuracy (88.95–101.18%) and precision (≤15%). Furthermore, the lower limit of quantification was found to be 4 μg/mL, with the lowest detection limit of 0.3217 μg/mL, indicating that this method is rapid, accurate, and reliable for the quantitative analysis of camrelizumab in human serum.
研究领域:
药理学
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分析化学
·
临床医学
·
质谱学
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药物开发
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2014
·
IF 3.107
Shuai-Shuai Hu,Ling Yi,Xing-Ying Li,Jun Cao,Li-Hong Ye,Wan Cao,Jian-Hua Da,Han-Bin Dai,Xiao-Juan Liu
An ionic liquid (IL)-based one-step micellar extraction procedure was developed for the extraction of multiclass polar analytes (protocatechuic acid, chlorogenic acid, epicatechin, hyperoside, isoquercitrin, quercetin) from hawthorn fruits and their determination using ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF/MS). Compared to conventional organic solvent extractions, this newly proposed method was much easier, more sensitive, environmentally friendly, and effective as well. Several important parameters influencing the micellar extraction efficiency are discussed, such as selection of ILs, surfactant concentration, and extraction time. Under the optimal conditions, good linearity was achieved for each analyte with correlation coefficients (r2) ranging from 0.9934 to 0.9999, and the recovery values ranged from 89.3 to 106% with relative standard deviations lower than 5.5%. Results suggest that the IL-based one-step micellar extraction could be an alternative and promising means in future food analysis.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Xi-Ming Wang,Zhi-Yong Guo,Yue Zhang,Ming-Li Chen,Jian-Hua Wang
Fe x O y and ZrO 2 nanoparticles co-doped layered porous polyimide , polyimide-Fe x O y -ZrO 2 is prepared with a one-step strategy, shortly termed as PI-Fe x O y -ZrO 2 . The layered and porous structure of the polymer offers a supported platform for metallic oxide anchoring, exhibiting a mesopore size of 3.93 nm and providing a surface area of 198.47 m 2 g −1 . The metallic oxides were uniformly and highly dispersed in the PI-Fe x O y -ZrO 2 nanocomposite with percentages of 15.81 and 20.53 wt% for Fe and Zr, respectively. The magnetic Fe x O y provides driving force for rapid separation. The high doping of ZrO 2 facilitates effective enrichment of phosphopeptides, even at a very low mass ratio of 1:1000 for tryptic digest of phosphopeptides/non-phosphopeptides, e.g., β-casein/BSA in this particular case. In addition, the PI-Fe x O y -ZrO 2 nanocomposite exhibits better adsorption performance to phosphopeptides with respect to commercial titanium dioxide nanoparticles . The effectiveness of low-abundant phosphopeptides isolation and enrichment from human serum is further identified and demonstrated by means of MALDI-TOF MS and LC-ESI-MS/MS.
研究领域:
生物化学
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材料科学
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分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD QUALITY
·
2024
·
IF 2.6
Menglu Yang,Yuan Mei,Haimei Lai,Yali Wang,Yuli Huang,Xueqing Zeng,Lihong Ge,Nan Zhao
Hotpot provides a multilevel unique flavor experience and is preferred by consumers who value various taste preferences. In the present study, results showed that alcohols, phenols, hydrocarbons, and others were the commonly predominant volatiles in all butter hotpot samples (74.43%∼92.92%). However, there were merely 25 codetected compounds determined among a total of 318 aroma compounds because of the discrepant ingredients and processing technique of hotpot samples, which were sampled from several representative manufacturers. Therefore, for the first time, multiple GC-Q-TOF/MS and GC-Orbitrap-MS methods were performed to explore the more 44 potential aroma compounds of butter hotpot, in which alpha-terpinyl, acetate, nonanal, piperitone, and (E)-lignstilde could further cause the differences in flavor intensity of spices aroma, smell of grease, and roasted, charred, and nutty (). Linalool and nerol were the critical flavor precursors associated with ingredients and processing technic. Therefore, these results provide guidance for improving the butter hotpot formula and process technique.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ACS ES&T Water
·
2024
·
IF 4.8
Jian Pan,Qizheng Zhuo,Xi-Zhi Niu,Yaxiong Zeng,Haiqiang Wang,Baohong Guan
Trace pharmaceutical active compounds (PhACs) in drinking water have been attracting wide attention due to their hazardous impacts and negative effects on human health and ecology, and their removal from drinking water has been becoming a priority. Here, we used the methods of UPLC-triple-TOF/MS to screen PhACs in the Shangxi Drinking Water Treatment Plant (DWTP) in Zhejiang, East China, and found the occurrence of ibuprofen, propranolol, and gemfibrozil. The current processes of DWTP cannot remove PhACs effectively. Gemfibrozil has the highest average concentrations among PhACs with 0.464, 1.292, and 0.583 ng/L at the units of sand filtration, ozonation-biological aerated filter (ozonation-BAF), and clean water pool, respectively. In a pilot-scale test, the ozonation removes 21.1–67.9% of gemfibrozil, while the catalytic ozonation with ceramic catalyst achieves removal efficiencies of 55.6–85.2% in 3.6 min. Gemfibrozil is degraded by catalytic ozonation through two pathways: initiated by ·OH reaction with aromatic ring C2 or started with ·OH attacking aromatic ring C3. This study reveals the existence of PhACs in DWTP and validates a feasible way of catalytic ozonation with a ceramic catalyst to remove them quickly.
研究领域:
环境科学
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公共卫生
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 5
·2415
·
IF 4.6
Ao Xu,Yunlin Xue,Yuyu Zeng,Jing Li,Huiling Zhou,Zhen Wang,Yin Chen,Hui Chen,Jian Jin,Tao Zhuang
Furosemide is a widely used loop diuretic in the treatment of congestive heart failure and edema. During the preparation of furosemide, a new process-related impurity G in the levels ranging from 0.08% to 0.13% was detected in pilot batches by a new high performance liquid chromatography (HPLC) method. The new impurity was isolated and characterized by comprehensive analysis of FT-IR, Q-TOF/LC-MS, 1D-NMR (1H,13C, and DEPT), and 2D-NMR (1H-1H-COSY, HSQC, and HMBC) spectroscopy data. The possible formation pathway of impurity G was also discussed in detail. Moreover, a novel HPLC method was developed and validated for the determination of impurity G and the other six known impurities registered in the European Pharmacopoeia as per ICH guidelines. The HPLC method was validated with respect to system suitability, linearity, the limit of quantitation, the limit of detection, precision, accuracy, and robustness. The characterization of impurity G and the validation of its quantitative HPLC method were reported for the first time in this paper. Finally, the toxicological properties of impurity G were predicted by the in silico webserver ProTox-II.Keywords:furosemide;process-related impurity;characterization;Q-TOF/LC–MS and NMR;method validation
研究领域:
药物化学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 21
·7320
·
IF 4.6
Abid Naeem,Chengqun Yu,Xiaoli Wang,Mingyan Peng,Yi Liu,Yali Liu
Radix Paeoniae Alba(RPA) has been used extensively in Chinese traditional medicine to treat gastrointestinal disorders, immune-modulating diseases, cancers, and numerous other conditions. A few of its active components include paeoniflorin, albiflorin, lactiflorin, and catechin. However, their therapeutic effectiveness is compromised by poor pharmacokinetic profiles, low oral bioavailability, short half-lives, and poor aqueous solubility. In this study, hydroxyethyl cellulose-grafted-2-acrylamido-2-methylpropane sulfonic acid (HEC-g-AMPS) hydrogels were successfully prepared for the controlled release ofRadix Paeonia Alba-solid dispersion (RPA-SD). A total of 43 compounds were identified in RPA-SD using UHPLC-Q-TOF-MS analysis. The hydrogel network formation was confirmed by FTIR, TGA, DSC, XRD, and SEM. Hydrogels’ swelling and drug release were slightly higher at pH 1.2 (43.31% swelling, 81.70% drug release) than at pH 7.4 (27.73% swelling, 72.46% drug release) after 48 h. The gel fraction, drug release time and mechanical strength of the hydrogels increased with increased polymer and monomer concentration. Furthermore, the hydrogels were porous (84.15% porosity) and biodegradable (8.9% weight loss per week). Moreover, the synthesized hydrogels exhibited excellent antimicrobial and antioxidative properties.Keywords:hydrogels;phytoconstituents;UHPLC-Q-TOF-MS;solid dispersion;traditional Chinese medicine;antimicrobial agents
研究领域:
药学
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材料科学
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传统医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Linna Song,Xiaohan Sun,Baohui Shi,Weiming Yang,Wen Ma
Condensed tannins are essential components in red wines, which not only contribute to their astringency effect but also determine wine quality. However, the evolution of tannins during vinification and wine aging remains unclear. Hence, this investigation analyzed the chemosensory characteristics of condensed tannins and their astringency perception in Marselan, Cabernet Sauvignon and MerlotCanalysis of condensed tannins and their astringency perception in Marselan, Cabernet Sauvignon and Merlotred wine during an industrial-scale winemaking procedure, via both non-targeted UPLC–Q-ToF and QDA techniques. The results indicated that Marselan exhibited the highest total tannins content, galloylation, prodelphinidins and astringency intensity among the three studied varieties. 13 tannins molecules were found to be the key molecule tannins (VIP>1) during Marselan aging evolution, whereas 14 and 15 molecule tannins were revealed as the key (VIP>1) in Cabernet Sauvignon and Merlot wines, respectively. Furthermore, the sensory results demonstrated that, after aging for 9 months, the astringency sub-quality of Merlot wine evolved at a slower rate than the other two. Additionally, astringency intensity was significantly positively ( p < 0.001) correlated with total tannins, mDP, and (E)G% for all three varieties, while astringency sub-quality was significantly negatively ( p < 0.001) correlated with total tannins and (E)G%, according to a correlation analysis of chemosensory metabolomic results. For Marselan, Cabernet Sauvignon, and Merlot, respectively, DP5-C, DP1-C, and the majority of key tannins (VIP>1) significantly adversely correlate with astringency sub-quality.
研究领域:
食品科学
·
感官分析
·
葡萄栽培学
✓ 已验证
产品关联
DOI
高影响因子
ARCHIVES OF MICROBIOLOGY
·
2024
·Vol. 206
·期 3
·1-17
·
IF 2.3
Wang Ze,Zhang Wenwen,Wang Ziyan,Zhang Zhixuan,Liu Yan,Liu Songyi,Wu Qiaoli,Saiding Emilaguli,Han Jiaojiao,Zhou Jun,Xu Jiajie,Yi Xianghua,Zhang Zhen,Wang Rixin,Su Xiurong
A new strain of Bacillus velezensis NDB was isolated from Xiangshan Harbor and antibacterial test revealed antibacterial activity of this strain against 12 major pathogenic bacteria. The whole genome of the bacterium was sequenced and found to consist of a 4,214,838 bp circular chromosome and a 7410 bp circular plasmid. Furthermore, it was predicted by AntiSMASH and BAGEL4 to have 12 clusters of secondary metabolism genes for the synthesis of the inhibitors, fengycin, bacillomycin, macrolactin H, bacillaene, and difficidin, and there were also five clusters encoding potentially novel antimicrobial substances, as well as three bacteriocin biosynthesis gene clusters of amylocyclicin, ComX1, and LCI. qRT-PCR revealed significant up-regulation of antimicrobial secondary metabolite synthesis genes after 24 h of antagonism with pathogenic bacteria. Furthermore, MALDI-TOF mass spectrometry revealed that it can secrete surfactin non-ribosomal peptide synthase and polyketide synthase to exert antibacterial effects. GC-MS was used to analyze methanol extract of B. velezensis NDB, a total of 68 compounds were identified and these metabolites include 16 amino acids, 17 acids, 3 amines, 11 sugars, 11 alcohols, 1 ester, and 9 other compounds which can inhibit pathogenic bacteria by initiating the antibiotic secretion pathway. A comparative genomic analysis of gene families showed that the specificity of B. velezensis NDB was mainly reflected in environmental adaptability. Overall, this research on B. velezensis NDB provides the basis for elucidating its biocontrol effect and promotes its future application as a probiotic.
研究领域:
微生物学
·
分子生物学
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Xi Gao,Yu Lu,Ming Wei,MeiFang Yang,CaiXia Zheng,Chengjian Wang,Ying Zhang,Linjuan Huang,Zhongfu Wang
The functional role of human milk oligosaccharides (HMOs) is closely associated with their type, composition, and structure. However, a detailed analysis of HMOs is difficult because neutral oligosaccharides (NHMOs) are mixed with sialylated oligosaccharides (SHMOs) in milk. Here, NHMOs were separated from SHMOs by DEAE-52 anion chromatography, and lactose was removed by graphite carbon solid-phase extraction. Lactose-free NHMOs were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) based on Girard’s reagent P on-target derivatization (GPOD), and SHMOs were analyzed by MALDI-TOF-MS following selective sialic acid derivatization and GPOD. Sixty-four oligosaccharides were detected: 36 NHMOs, of which 28 were fucosylated, and 28 SHMOs, of which 8 with α-2,3-linked monosialic acid, 2 with α-2,3-linked disialic acid, 10 with α-2,6-linked monosialic acid, 2 with α-2,6-linked disialic acid, and 5 with both α-2,3- and α-2,6-linked disialic acid. These findings provide the groundwork for further characterization of the structure and activity of HMOs.
研究领域:
生物化学
·
营养学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Applied Spectroscopy
·
2019
·Vol. 86
·期 1
·89-95
·
IF 0.675
Wang Haojiang,Wang Xuan,Bian Wei,Sun Tijian,Cai Zongwei,Wei Jiancong
Core-shell magnetic metal organic nanocrystals were synthesized and used as matrix for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four nitropolycyclic aromatic hydrocarbons (nitro-PAHs) were successfully detected by using negative-ion MALDI-TOF MS without background interference. Furthermore, the magnetic nanocomposites (MNCs) coated with zeolitic imidazolate framework-8 (ZIF-8) showed excellent adsorption and enrichment capacity, and can be isolated with a magnet and directly spotted on the stainless steel plate for MALDI measurement. It was also analyzed nitro-PAHs in PM2.5 samples using the ZIF-8-coated magnetic nanocomposite as adsorbent and matrix.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）