MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-10
·
IF 5.705
Xu Sai,Yu Yang,Gao Yuefeng,Zhang Yanqiu,Li Xiangping,Zhang Jinsu,Wang Yunfeng,Chen Baojiu
Accurate detection of temperature and oxygen concentration at the cellular level is important in tumor diagnosis. Multifunctional nanocomposites are described that consist of upconversion luminescent nanoparticles capped with mesoporous silica and loaded with an oxygen-sensitive luminescent ruthenium complex. The nanocomposites of type NaYF 4 :Yb,Er@NaYF 4 @mSiO 2 -Ru have two modes of operation: Its red downconversion luminescence (at excitation/emission peaks of 455/606 nm) is quenched by oxygen (O 2 ), and this is used to sense and image O 2 . The green upconversion luminescence (typically acquired at excitation/emission wavelengths of 980/525 and 544 nm), in turn, is used to measure temperature. The nanocomposites were then applied to dual mode in-vitro imaging of temperature and O 2 in hepatocellular carcinoma cells (HepG-2).
A new multifunctional nanocomposite of mesoporous silica coating NaYF 4 :Yb,Er@NaYF 4 upconversion nanopaticless loaded with ruthenium (II) complex has been designed with both green upconversion and red downconversion luminescence for temperature detection, oxygen sensing and dual mode cell imaging.
研究领域:
生物医学工程
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2024
·
IF 3.8
Mengran Ouyang,Huimin Du,Jiahao Wu,Shanshan Hu,Jun Yang
KSrGd(PO 4 ) 2 :Ln 3+ (Ln = Ce, Sm, Eu, Tb, Dy, Dy/Eu, Yb/Ho, Yb/Er, Yb/Tm) phosphors were synthesized by high temperature solid state method. Doping the KSrGd(PO 4 ) 2 matrix with 5%Dy 3+ ions resulted in white light emission under 345 nm excitation. The calculated color temperature (CCT) of this white light is 5523 K, indicating as positive white light. It was found that the luminous intensity of the obtained KSrGd(PO 4 ) 2 :5%Dy 3+ at 200 °C was 76.80 % of that at room temperature, showing good thermal stability as well as possibility of application in white LED. Under the excitation at 392 nm, the fluorescence quantum yield of KSrGd(PO 4 ) 2 :10%Eu 3+ is 79.92 %. Energy transfer between Dy 3+ and Eu 3+ is observed in KSrGd(PO 4 ) 2 :Dy 3+ /Eu 3+ samples, with identified mechanism as the quadrupole-quadrupole interaction. Tunable luminescence ranging from white to yellow then to orange-red is achieved by adjusting the doping concentration of Eu 3+ in KSrGd(PO 4 ) 2 :5%Dy 3+ , x%Eu 3+ . Under the excitation of 980 nm near-infrared light, the prepared KSrGd(PO 4 ) 2 :Yb 3+ /Ho 3+ , KSrGd(PO 4 ) 2 :Yb 3+ /Er 3+ and KSrGd(PO 4 ) 2 :Yb 3+ /Tm 3+ samples showed up-conversion emissions of red, yellow and blue, respectively, with two-photon processes. In KSrGd(PO 4 ) 2 matrix, multi-color and multi-security marks were successfully prepared by utilizing up/down conversion luminescence characteristics, which obtained good anti-counterfeiting effect and improved anti-counterfeiting security. Therefore, the obtained KSrGd(PO 4 ) 2 : Ln 3+ phosphors exhibit promising application prospects in the fields of WLED and multiple anti-counterfeiting.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
CRYSTENGCOMM
·
2020
·Vol. 22
·期 38
·6330-6338
·
IF 3.117
Chunyan Cheng,Yueshan Xu,Gejihu De,Jianxun Wang,Wei Wu,Yuexing Tian,Shasha Wang
Rare-earth doped upconversion nanoparticles (UCNPs) have attracted wide-ranging interest due to their potential applications in bioimaging and biolabeling, where a high emission intensity and small particle size are needed. However, common synthesis processes can be somewhat complex or require additional reactants, and the use of small-size NPs is limited by their weak luminescence intensity. In this work, uniform, monodisperse ∼5 nm β-NaGdF4:20% Yb/2% Er NPs were synthesized via a simple one-pot synthesis method in the high boiling-point solvents 1-octadecene (ODE) and oleic acid (OA) using a single dose of NaF at an ultralow NaF concentration of NaF/RE = 4. The effects of NaF/RE, the reaction temperature, and the reaction time on the size and phase of the particles were investigated. High-quality β-NaGdF4:20% Yb/2% Er@β-NaGdF4:20% Yb/10% Nd core–shell NPs with a size of ∼13 nm, enhanced UC luminescence, long UC lifetimes (τ) for green (τ ≈ 150 μs) and red (τ ≈ 105 μs) emission under 980 and 808 nm excitation, revealed via lifetime imaging, and obvious emission in the near-infrared (NIR) II region were synthesized. The synthesized core–shell NPs may result in a decrease in the overheating effect and allow for a deep penetration depth when further used for bioimaging applications. This work could inspire the synthesis of other high-quality RE-doped UCNPs via adding a single dose of a small amount of NaF and could lay the foundation for the use of Gd-based UCNPs in biomedical applications.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 7
·1-8
·
IF 6.232
Wu Nengying,Wei Yuxi,Pan Lanlan,Yang Xiaolin,Qi Honglan,Gao Qiang,Zhang Chengxiao
Lateral flow immunostrips were newly designed and a sensitive and rapid fluorometric method for the determination of 8-hydroxy-2′-deoxyguanosine (8-OHdG) as a model target of small biomarker molecules was developed. The upconversion nanoparticles (UCNPs, NaYF4:Yb/Er core, and polyacrylic acid (PAA)-modified shell, size ~ 39 nm, excitation wavelength = 980 nm; emission wavelength = 540 nm) were employed as fluorescence signal material. The 8-OHdG antibody (Ab) was taken as the recognition probe while UCNP-labeled Ab was taken as the signal probe. Bovine serum albumin (BSA) was designed as carrier protein for 8-OHdG to form 8-OHdG-BSA conjugate as the capture probe. The lateral flow immunostrips were prepared by laminating a sample pad (glass fiber membrane), a test pad (nitrocellulose membrane), and adsorption pad (filter paper) on PVP backing. The capture probe was immobilized on the test zone while an IgG antibody taken as the control probe was immobilized on the control zone. When the signal probe and the sample were in sequence loaded on the sample pad, 8-OHdG analyte bound with the signal probe, and then the excess of the signal probe move along the strip and is collected by the capture probe on the test zone while the remnant signal probe is collected by the control probe on the control zone. The signal probe and capture probe were synthesized and characterized. The fluorescence intensity on the test zone was inversely proportional to the concentration of 8-OHdG for the quantitative determination while the fluorescence emission on the control zone was observed to validate the assay. The developed method showed a wide linear range from 0.10 to 10 nM, a quite low detection limit of 0.05 nM, small sample volume requirement (100 μL), short assay time (15 min), and good method reproducibility (RSD = 4.4%, nine immunostrips).
Schematic illustration of the configuration and measurement principle of lateral flow fluorescence immunostrip for 8-OHdG: (a) configuration; (b) preparation: load of capture probe (BSA-8-OHdG, 2 μL) on test zone; load of control probe (IgG Ab, 2 μL) on control zone; load of signal probe (UCNP-Ab, 16 μL) on sample pad; (c) measurement: load of sample (8-OHdG, 100 μL) on sample pad, collection, and measurement.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）