FOOD CONTROL
·
2024
·
IF 5.6
Lianjun Song,Meijun Lu,Puye Liang,Tiange Li,Xianqing Huang,Yan Ma,Libin Wan,Tianlin Wang
Herein, a ratiometric fluorescence aptasensor was established for zearalenone (ZEN) detection based on fluorescence resonance energy transfer (FRET) between aptamer-modified CdTe quantum dots (QDs) as donor and gold nanoparticles (AuNPs) as acceptor. The fluorescence of CdTe QDs quenched by AuNPs based on FRET, while Si QDs with blue fluorescence as reference. The aptamer-modified CdTe QDs were adsorbed to ZEN in the presence of ZEN, and the distance between the energy receptor and the donor increased, which prevented the FRET and led to the fluorescence recovering of CdTe QDs. The aptasensor exhibited a limit of detection of 3.5 pg/mL with a linear range of 0.01–100 ng/mL. In addition, a portable detection device was constructed by integrating with smartphone for point-of-care testing (POCT) of ZEN with satisfactory results, which could provide a promising application for visual POCT of ZEN in cereals.
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高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yihao Li,Fengzhen Jin,Bowen Li,Shun Wu,Yuanqing Wang,Yaohui Wu,Yonghong Wang,Ge Ning
Zearalenone (ZEN) is a mycotoxin that exerts multiple toxic effects on humans through the contamination of grains such as corn, posing a significant threat to food safety and human health. This work proposed a dual-signal ratiometric electrochemical aptasensor utilizing ferrocene-carboxylic acid‑gold nanoparticle‑molybdenum disulfide nanosheets (Fc-Au-MoS 2 ), hybridization chain reaction (HCR), and the cleavage effect of DNAzyme triggered by triple-helix molecular switch (THMS) to detect ZEN. The specific interaction with ZEN triggers the release of the DNAzyme from THMS, which then removes the immobilized primer chain on the electrode surface with the assistance of Pb 2+ , thereby preventing HCR occurrence. This inhibitory mechanism reduces methylene blue (MB) insertion while exposing the active sites of Fc-Au-MoS 2 , generating an opposite signal response to achieve high-precision ratio detection. The aptasensor demonstrated a low detection limit of 29.4 pM, with satisfactory recoveries in corn flour (97.3 %–106.4 %), confirming its potential for monitoring mycotoxin residues in food safety.
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高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 7
·503
·
IF 5.4
Xiao Yue,Wen Xuefei,Zhang Xingzhong,Chen Hongrui,Tang Jie,Chi Hai,Li Liman,Thanapop Soteyome
To enable sensitive quantitative detection of zearalenone (ZEN) in cereal matrices, this study develops a molecularly imprinted electrochemical sensor. A three-dimensional flower-shaped carbon composite material (CoNiZn@C), synthesized from a ternary CoNiZn metal–organic framework, was utilized as a highly conductive substrate featuring excellent electrical conductivity and abundant binding sites. Density functional theory calculations were conducted to elucidate the intermolecular interactions and cooperative recognition mechanisms between zearalenone and the selected monomers. Experimentally, the AuNPs-based thioaniline units (PATP@AuNPs) and pyrrole were employed as bifunctional monomers. Specifically, PATP@AuNPs can be stably immobilized on the substrate surface to enhance interfacial electron transfer efficiency, while simultaneous in situ copolymerization of the two monomers generates numerous non-covalent binding cavities capable of specifically recognizing ZEN. Under optimized experimental conditions, the sensor exhibited a linear detection range from 10 nM to 1000 µM and a detection limit as low as 1.6 nM. This detection approach is straightforward, cost-effective, and demonstrates excellent specificity and sensitivity, indicating substantial potential for rapid screening and quantitative analysis of mycotoxins in agricultural products. Graphical
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Bofei Zhang,Ke Zhao,Baozhong Zhang,Boxu Chen,Kunfeng Zhang,Hanyu Chen,Baoshan He
A dual-mode (colorimetric/electrochemical) aptasensor was developed for ZEN detection. • Au@Ti 3 C 2 T x MXene@MoSe 2 was synthesized to enhance electrode conductivity and surface area. • RCA was integrated to generate G-quadruplexes for both electrochemical and colorimetric readouts. • Electrochemical mode achieved 1.23 fg/mL LOD; colorimetric mode enabled convenient visual screening. • The sensor exhibited excellent selectivity, stability, and recoveries (89.6–106.2%) in real samples. Zearalenone (ZEN), a nonsteroidal mycotoxin produced by Fusarium fungi, widely contaminates cereals and their derived products, posing a serious threat to human health. In this study, a highly sensitive dual-mode (colorimetric and electrochemical) aptasensor for ZEN was constructed. The sensor employs rolling circle amplification (RCA) to efficiently generate long single-stranded DNA rich in G-quadruplex sequences, combined with an electrode modified with an Au@Ti 3 C 2 T x MXene@MoSe 2 nanocomposite, thereby achieving synergistic signal amplification and dual readout. In the electrochemical mode, the RCA product is anchored through hybridization between the primer and the capture probe on the electrode surface, where it adsorbs abundant methylene blue to produce a square wave voltammetry (SWV) response. In the colorimetric mode, the G-quadruplex structures self-assemble with hemin to form peroxidase-mimicking DNAzymes, which catalyze the TMB chromogenic reaction to generate a visual signal. Experimental results showed that the electrochemical mode achieved a detection limit as low as 1.23 fg/mL (detection range: 10 −3 -10 4 pg/mL), while the colorimetric mode exhibited a detection limit of 64.89 fg/mL (detection range: 1 pM-10 μM). The aptasensor demonstrated excellent selectivity, good stability, and satisfactory reproducibility for ZEN, with spiked recoveries ranging from 89.6% to 106.2% in real corn flour and beer samples. This dual-mode sensing platform effectively eliminates single-mode interferences through signal cross-validation, providing a reliable technical means for the accurate and rapid detection of trace ZEN in food safety. Download: Download high-res image (117KB) Download: Download full-size image
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JOURNAL OF FOOD SCIENCE
·
2023
·Vol. 88
·期 6
·2723-2734
·
IF 3.9
Jiao Tian,Jiaoyang Luo,Jiaan Qin,Yudan Wang,Xinqi Sun,Jing Zhang,Tongwei Ke,Mengyue Guo,Haonan Ruan,Fang An,Meihua Yang
The purpose of this study was to prepare a highly sensitive and specific zearalenone (ZEN) monoclonal antibody, which was then used to develop an indirect enzyme-linked immunosorbent assay (ic-ELISA) and a colloidal gold immunochromatographic assay (GICA). These techniques were used for the detection of Coicis Semen and related products (Coicis Semen flour, Yimigao, and Yishigao). Immunogens were synthesized by oxime active ester techniques and characterized via ultraviolet spectrophotometry. Immunogens were injected subcutaneously into the abdominal cavities and backs of mice. Using the prepared antibodies, we developed ic-ELISA and GICA rapid detection methods, which were then applied for the rapid detection of ZEN and its analogues from Coicis Semen and related products. For ic-ELISA, the half maximal inhibitory concentration (IC 50 ) values for ZEN, α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), zearalanone (ZAN), α-zearalanol (α-ZAL), and β-zearalanol (β-ZAL) were determined to be 1.13, 1.69, 2.06, 0.66, 1.20, and 0.94 ng•mL −1 , respectively. For GICA, the cutoff values of ZEN, α-ZEL, β-ZEL, α-ZAL, and β-ZAL on test strips were 0.5 ng•mL −1 in phosphate buffer saline (0.01 M, pH 7.4), while ZAN was found to be 0.25 ng•mL −1 . Furthermore, the cutoff values of test strips were between 10 and 20 µg∙kg −1 in Coicis Semen and related products. The results of these two detection methods were in good agreement with results from liquid chromatography-tandem mass spectrometry. This study provides technical support for the preparation of broad-specificity monoclonal antibodies against ZEN and lays the foundation for the simultaneous detection of multiple mycotoxins from food and herbal medicines.
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高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Mei Liu,Jinxin Zhang,Shasha Liu,Baoxin Li
ZEN-responsive hydrogel was prepared using ZEN aptamer and a cationic conjugated skeleton which was formed by the electrostatic interaction between acrylamide and poly (diallyldimethylammonium chloride) (PDDA). In the presence of ZEN, the binding of aptamer and ZEN caused the hydrogel to dissociate, releasing the gold nanoparticles (AuNPs) encapsulated in the hydrogel into supernatant, whose color changes serving as response instructions were observed by eyes. To realize low dose visual detection, the TMB-H 2 O 2 was used for quantitative readout by AuNPs released from the hydrogel which can efficiently catalyze the reaction to generate a obvious change.There was a good linear relationship between the changed absorbance and concentration of ZEN within 2.5–100 ng/mL, a detection limit of 0.98 ng/mL, recovery rates of 98.8%-101.3% and 99.8%-101.5% for corns and beer, respectively. This developed sensing strategy would provide a promising application for other mycotoxins by replacing the corresponding aptamer sequences.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 8
·1-9
·
IF 5.833
Chen Ruipeng,Li Shuang,Sun Yunfeng,Huo Bingyang,Xia Yating,Qin Yingkai,Li Shuning,Shi Baodi,He Defu,Liang Jun,Gao Zhixian
The design and fabrication of a surface-enhanced Raman scattering (SERS) aptasensor for simultaneous detection of zearalenone (ZEN) and ochratoxin A (OTA) in wheat and corn samples is described. The capture and reporter probes were SH-cDNA-modified gold nanorods and SH-Apt-modified Au@Ag core-shell nanoparticles, respectively. After recognizing OTA and ZEN aptamers and complementary strands (SH-cDNA), the reporter probe generated a strong SERS signal. The preferred binding of OTA and ZEN aptamers to OTA and ZEN, respectively, caused reporter probes to release the capture probes, resulting in a linear decrease in SERS intensity. The detection of OTA showed good linearity with an R 2 value of 0.986, which could be maintained across a wide concentration range (0.01 to 100 ng/mL), with the limit of detection of 0.018 ng/mL. For detection of ZEN, good linearity with an R 2 value of 0.987 could be maintained across a wide concentration range (0.05 to 500 ng/mL), with 0.054 ng/mL as the limit of detection. Good accuracy (relative standard deviation < 4.2%) during mycotoxin determination as well as excellent quantitative recoveries (96.0–110.7%) during the analysis of spiked real samples was achieved. The proposed SERS aptasensor exhibited excellent performance in the detection of OTA and ZEN in real food samples. Hence, by simply changing the aptamer, this new model can be applied to the detection of multiple mycotoxins in the food industry. Graphical abstract
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POLYMER BULLETIN
·
2019
·Vol. 77
·期 9
·4507-4522
·
IF 1.858
Liu Huijun,Zhang Jianing,Ding Ke,Chen Xiangning,Han Tao
This study aimed to establish a purifying immunoaffinity column (IAC) for four kinds of Fusarium toxins (zearalenone, deoxynivalenol, T-2, and HT-2) simultaneously using poly-glycidyl methacrylate–divinylbenzene [P(GMA–DVB)] as the column matrix. The P(GMA–DVB) microsphere was synthesized using glycidyl methacrylate (GMA) and diethylbenzene (DVB) at an optimal polymerization temperature of 80 °C, the initiator 2,2′-azodiisobutyronitrile concentration of 2.50 g/mL H 2 O, and the DVB-to-GMA ratio of 3:5 (wt.). The polymerized P(GMA–DVB) microspheres were modified with 1,6-hexanediamine, activated with glutaraldehyde, and coupled with the four toxin monoclonal antibodies. The coupling rate of the activated resin was calculated to be 95.95%. The maximum capacity of the IACs was 264 ng deoxynivalenol, 231 ng T-2, 210 ng HT-2, and 292 ng zearalenone. After eight uses, the maximum capacity had no obvious decrease. The recovery of the four Fusarium toxins on the P(GMA–DVB) IAC was 70.03–85.39% in flour, with the relative standard deviations of 4.67–15.93%. The IACs were applied to the actual flour samples.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 8
·1-10
·
IF 5.479
Niazi Sobia,Khan Imran Mahmood,Yu Ye,Pasha Imran,Shoaib Muhammad,Mohsin Ali,Mushtaq Bilal Sajid,Akhtar Wasim,Wang Zhouping
A “turn on” time-resolved fluorometric aptasensor is described for the simultaneous detection of zearalenone (ZEN), trichothecenes A (T-2), and aflatoxin B 1 (AFB 1 ). Multicolor-emissive nanoparticles doped with lanthanide ions (Dy 3+ , Tb 3+ , Eu 3+ ) were functionalized with respective aptamers and applied as a bioprobe, and tungsten disulfide (WS 2 ) nanosheets are used as a quencher of time-resolved fluorescence. The assay exploits the quenching efficiency of WS 2 and the interactions between WS 2 and the respective DNA aptamers. The simultaneous recognition of the three mycotoxins can be performed in a single solution. In the absence of targets, WS 2 is easily adsorbed by the mixed bioprobes via van der Waals forces between nucleobases and the WS 2 basal plane. This brings the bioprobe and WS 2 into close proximity and results in quenched fluorescence. In the presence of targets, the fluorescence of the bioprobes is restored because the analytes react with DNA probe and modify their molecular conformation to weaken the interaction between the DNAs and WS 2 . Under the optimum conditions and at an excitation wavelength of 273 nm, the time-resolved fluorescence intensities (peaking at 488, 544 and 618 nm and corresponding to emissions of Dy 3+ , Tb 3+ and Eu 3+ ) were used to quantify ZEN, T-2 and AFB 1 , respectively, with detection limits of 0.51, 0.33 and 0.40 pg mL −1 and a linear range from 0.001 to 100 ng mL −1 . The three mycotoxins can be detected simultaneously without mutual interference. The assay was applied to the quantification of ZEN, T-2 and AFB 1 in (spiked) maize samples. This homogeneous aptamer based assay can be performed within 1 h. Conceivably, it can become an alternative to other heterogeneous methods such as the respective enzyme-linked immunosorbent assays.
Schematic presentation of an aptasensor for simultaneous detection of zearalenone, trichothecenes A and aflatoxin B 1 using aptamer modified time-resolved fluorescence nanoparticles as signalling probes and tungsten disulfide as the quencher. This assay shows lower detection limit and requires no washing steps.
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高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Liangliang Peng,Yao Yao,Meiqi Li,Xingtong Ji,Jinbo Cao,Jiaming Zhang,Fusheng Zhong,Xixiang Yang,Xiaogang Hu
Zearalenone (ZEN), a mycotoxin produced by fungi of the genus Fusarium , is widely present in animal feeds and human foods, posing a serious hazard to human health. Herein, a zearalenone aptamer-functionalized magnetic metal-organic framework material (ZEN-Apt@MMIL-100(Fe)) was constructed and applied for the rapid enrichment of ZEN, coupled with high-performance liquid chromatography (HPLC) for ultrasensitive detection. By a self-templating method, magnetic MIL-100(Fe) was formed by self-assembly of sodium citrate-modified Fe 3 O 4 particles as nuclei with homobenzoic acid, which was bonded to amino-modified zearalenone nucleic acid aptamer via amide reaction to realize a specific recognition function. The magnetic dispersive solid-phase extraction and HPLC method was established using this material, with the linear range of 5 to 200 μg/L ( R 2 = 0 . 997) and the limit of detection of 2.92 μg/L. The method was applied to detect ZEN in maize and wheat samples, with spiked recoveries ranging from 81.7% to 98.2% at spiking levels of 25 μg/L, 50 μg/L, and 100 μg/L. The method is able to realize the selective and rapid enrichment and detection of trace ZEN in wheat and maize through the synergistic action of aptamer-specific recognition and magnetic MOFs, which provides a sensitive and reliable analytical strategy for food security monitoring.
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Reproductive Biology
·
2023
·
IF 2.1
Yulu Cao,Jingjing Wang,Xiaoxiao Li,Biyun Liu,Chongjun Li,Yijin Sun,Kang Zou
Zearalenone (ZEA) is a prevalent mycotoxin found in moldy diets and is associated with reproductive dysfunction. However, the molecular underpinning of ZEA in impairment of spermatogenesis remains largely unknown. To unveil the toxic mechanism of ZEA, we established a co-culture model using porcine Sertoli cells and porcine spermatogonial stem cells (pSSCs) to investigate the impact of ZEA on these cell types and their associated signaling pathways . Our findings showed that low concentration of ZEA inhibited cell apoptosis , while high concentration induced cell apoptosis. Furthermore, the expression levels of Wilms’ tumor 1 (WT1), proliferating cell nuclear antigen (PCNA) and glial cell line-derived neurotrophic factor (GDNF) were significantly decreased in ZEA treatment group, while concurrently upregulating the transcriptional levels of the NOTCH signaling pathway target genes HES1 and HEY1 . The addition of the NOTCH signaling pathway inhibitor DAPT (GSI-IX) alleviated the damage to porcine Sertoli cells caused by ZEA. Gastrodin (GAS) significantly increased the expression levels of WT1, PCNA and GDNF, and inhibited the transcription of HES1 and HEY1 . GAS also efficiently restored the decreased expression levels of DDX4, PCNA and PGP9.5 in co-cultured pSSCs suggesting its potential in ameliorating the damage caused by ZEA to Sertoli cells and pSSCs. In conclusion, the present study demonstrates that ZEA disrupts pSSCs self-renewal by affecting the function of porcine Sertoli cell, and highlights the protective mechanism of GAS through the regulation of the NOTCH signaling pathway. These findings may offer a novel strategy for alleviating ZEA-induced male reproductive dysfunction in animal production.
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高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2024
·
IF 3.9
Yanan Wang,Qianqian Wang,Gaigai Wang,Qiongqiong Zhang,Yongpeng Guo,Xin Su,Yu Tang,Matthew Koci,Jianyun Zhang,Qiugang Ma,Lihong Zhao
Zearalenone (ZEN) poses a potential threat on human and animal health partly through the nuclear factor (NF)-κB signaling pathway. In silico study suggested that rutin effective against TLR4 and NF-κB. A wetting test was designed to evaluate the effect and underlying mechanism of rutin in alleviating ZEN-induced inflammation in animals. Twenty-four female mice were randomly divided into 4 groups: control (basal diet), ZEN group (basal diet + ZEN), rutin group (basic diet + rutin), Z + R group (basal diet + rutin + ZEN). Results showed that rutin effectively alleviated ZEN-induced inflammation and damage of liver and jejunum in mice. Rutin addition reduced the content of lipopolysaccharide (LPS) in serum and liver mainly by improving the intestinal barrier function resulted from the production increase of short-chain fatty acids (SCFA). In sum, this study showed that rutin alleviated ZEN-induced liver inflammation and injury by modulating the gut microbiota, increasing the production of SCFA and improving intestinal barrier function, leading to the decrease of LPS in liver and the inhibition of MyD88 independent NF-κB signaling pathway in mice. Specifically, these findings may provide useful insights into the screening of functional natural compounds and its action mechanism to alleviate ZEN induced liver inflammation.
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