INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Kehui Wang,Xiangjun Yang,Junjun Liang,Yan Rong,Weijie Zhao,Jiahao Ding,Yiming Liu,Qi Liu
This study employed a precipitation method to synthesize zinc oxide@quaternised chitosan nanoparticles (ZnO@QAC NPs) containing different concentrations of zinc oxide, namely ZnO@QAC-2, ZnO@QAC-4, and ZnO@QAC-6. Subsequently, these nanoparticles were incorporated into matrices consisting of gelatine (Gn) and polyvinyl alcohol (PVA) separately, which were prepared by casting to form a biodegradable film. We assessed the physicochemical properties of ZnO@QAC NPs and physicochemical characteristics, antioxidant properties, antimicrobial activity and grape preservation efficacy of the film. Compared to the control group, the films showed a reduction in water vapor permeability by >9.38 %, an increase in tensile strength by over 51.95 %, over 70 % scavenging of ABTS free radicals, and good biocompatibility. Additionally, the antimicrobial activity of the films containing ZnO@QAC-6 increased by 37.6 %. In the grape preservation experiment, the weight loss of grapes wrapped in ZnO@QAC-2 film was reduced by 40.13 % on day 15 compared to unwrapped grapes. These results demonstrate that ZnO@QAC/PVA/Gn films have considerable potential for food packaging applications.
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ENVIRONMENTAL RESEARCH
·
2026
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IF 8.2
Qi Zeng,Zhichao Shi,Shijing Deng,Tingbiao Wu,Lihong Wu,Qiaoqiao Guo,Jianping Yin,Tingting Fan,Xinpeng Tian,Qinglian Li
The accumulation of microplastics in marine environments poses a significant ecological threat. This study reports the isolation and characterization of a marine-derived fungus, Cladosporium oxysporum SCSIO 81042, capable of degrading polyurethane (PU) under seawater conditions. Through tiered screening, the strain was selected based on its superior hydrolysis activity against Impranil® DLN and solid poly(1,4-butylene adipate) PBA-based PU (PBA-PU). The strain demonstrated exceptional adaptability to seawater conditions, with optimal growth at 15–28 °C, ∼3% salinity, and pH 7.5–8.0. Under simulated seawater salinity, it degraded 94.5% of Impranil® DLN within four days in simulated seawater while maintaining sustained activity over two successive cycles. In natural seawater, near-complete degradation of 0.1% Impranil® DLN was achieved within 7 days, and the degradation products showed negligible developmental toxicity to zebrafish. Notably, this efficiency successfully translated to a 10-L seawater mesocosm, where most Impranil® DLN was degraded within 7 days. The strain also caused structural damage to solid PBA-PU films and PU foam under simulated seawater salinity after 14 days, although no significant weight loss was observed. Metabolite analysis confirmed its ability to hydrolyze both ester and urethane bonds in PBA-PU. Furthermore, chitosan nanoparticle (CS-NP) was introduced as an adhesion adjuvant, enhancing degradation across all tested PU substrates, potentially through facilitated microbial adhesion and substrate contact. While challenges remain regarding degradation of complex PU foams, CS-NP environmental fate, and microbial competition, this work establishes C. oxysporum SCSIO 81042 and the CS-NP adjuvant strategy as promising tools for the bioremediation of marine PU pollution.
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CARBOHYDRATE POLYMERS
·
2023
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IF 11.2
Licong Yang,Yabin Wang,Zhiwei Li,Xiaohua Wu,Jingtao Mei,Guodong Zheng
The pathology of Alzheimer's disease (AD) is highly correlated with obesity-induced insulin resistance. Resveratrol (Res) is a natural phenol that demonstrates a neuroprotective effect, but the bioactivity of Res is low in vivo . Here, chitosan (CS) was cross-linked with sodium tripolyphosphate (TPP) to encapsulate low water solubility Res. Next, a brain-targeted peptide (TG: TGNYKALHPHNG) was modified on the surface of Res-loaded CS/TPP nanoparticles (TG-Res-CS/TPP-NPs) to specifically deliver Res to the brain. Morris water maze results indicated that cognitive impairments were ameliorated by TG-Res-CS/TPP-NPs in obesity-related AD mice. Obesity-related insulin resistance promotes Tau phosphorylation and Aβ aggregation in the brain. Administration of TG-Res-CS/TPP-NPs alleviated lipid deposition-induced insulin resistance and decreased the level of phosphorylated Tau and Aβ aggregation via the JNK/AKT/GSK3β pathway. Additionally, TG-Res-CS/TPP-NPs transported across blood-brain barrier which in turn increased glucose transporter expression levels, antioxidant enzyme activity and inhibited microglial cell activation . Thus, TG-Res-CS/TPP-NPs were more effective than Res-CS/TPP-NPs at regulating glucose homeostasis , oxidative stress and neuroinflammation in the brain. Moreover, inflammatory , lipid metabolism and oxidative stress-related gut microbiota including Helicobacter , Colidextribacter , Anaerotruncus , Parasutterella , Allobaculum , Alloprevotella , Alistipes , Bifidobacterium and Candidatus_Saccharimonas were also regulated by TG-Res-CS/TPP-NPs. This work indicates the potential use of TG-Res-CS/TPP-NPs for the delivery of Res.
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EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
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2021
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IF 4.384
Yaowen Wang,Jingjing Ma,Tong Qiu,Mingxiu Tang,Xueqiong Zhang,Weiguo Dong
Background Oxaliplatin (OXE) combined with other chemotherapy drugs against colorectal cancer had been reported in the literature before, however, the efficacy of oxaliplatin combined with natural compounds was elusive. In addition, the clinical bioactivity and therapeutic dose of antitumor drugs are severely limited due to poor targeting and side effects. NDDSs offers an excellent strategy to overcome the disadvantages of small molecule anticancer drugs. Methods Here, we have prepared N,O-carboxymethyl chitosan Oxaliplatin nanoparticles (CMCS-OXE NPs) and N,O-carboxymethyl chitosan Resveratrol nanoparticles (CMCS-Res NPs) were prepared by ion crosslinking and emulsification crosslinking, respectively. Results The results revealed that the CMCS-OXE NPs exhibited a high encapsulation efficiency (60%) with a size of approximately 190.0 nm , and the CMCS-Res NPs exhibited a high encapsulation efficiency (65%) with a size of approximately 164.2 nm. The treatment with both types of nanoparticles combined exhibited more significant anti-colon cancer activity than the free drugs or either type of nanoparticle alone. In the in vivo experiments, the inhibition efficiency of the combined nanoparticle treatment was much stronger than the free drugs or either type of nanoparticle alone. Conclusions Overall, combination of oxaliplatin and resveratrol into a nanoparticle-drug delivery systems (NDDSs) appears to be a promising strategy for colorectal cancer (CRC) therapy.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
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IF 5.162
Minghui Sun,Qianjin Xie,Xiaqiang Cai,Zenghui Liu,Ying Wang,Xu Dong,Yan Xu
The wounds of diabetic patients are difficult to heal, which could lead to a limb amputation or even death. The experiment aims to develop a new type of nanoparticles that could accelerate wound healing. Epigallocatechin gallate, ascorbic acid, gelatin and chitosan nanoparticles (EV NPS) were prepared by ion cross-linking method, and their properties were studied. The optimal formula ratio of EV NPS is Vc:EGCG:Gel:CS = 0.2:3:1:1. Transmission electron microscope (TEM) images show that it is a roughly uniform spherical nanoparticle with a diameter of 200 nm. ICR mice were intraperitoneally injected with streptozocin (STZ) to establish diabetic mice. Full-thickness excisional wounds were established on the back of mice. The results showed that EV NPS can promote wound healing in diabetic mice, and the mechanism may be through increasing collagen accumulation, promoting angiogenesis and reducing the infiltration of inflammatory cells. EV NPS may have potential application values for wound healing in diabetic mice.
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Nanomedicine-Nanotechnology Biology and Medicine
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2019
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IF 5.57
Xiaoyan Niu,Jing Cao,Yapei Zhang,Xuefeng Gao,Mingbo Cheng,Yang Liu,Wei Wang,Zhi Yuan
The combination therapy of nitric oxide (NO) and anticancer drug was developed for reversing multidrug resistance (MDR). In order to avoid NO release during the blood circulation, and realize pinpointed release in the tumor cells, we designed a tumor-specific NO-release system based on 10-hydroxycamptothecin (HCPT)-loaded charge-reversal chitosan nanoparticles and covalently linked phenylsulfonyl furoxan (glutathione (GSH)-responsive NO donor) on the surface. The results showed that only 6.0% of NO was eventually released under physiology condition (pH 7.4 and 2 μM GSH) within 8 h. In contrast, 93.0% of NO was released within 4 h in the presence of 10 mM GSH. Western blot result displayed the P-glycoprotein expression was significantly decreased by 50.1%. Hence, this system performed remarkable cytotoxicity in vitro and the highest tumor inhibition rate (79.7%) comparing with free HCPT group (20.7%) in vivo . Such GSH-responsive NO-release system is a promising candidate with prominent therapeutic effect against MDR tumor.
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CARBOHYDRATE POLYMERS
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2024
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IF 10.7
Rahila Hafeez,Junning Guo,Temoor Ahmed,Hubiao Jiang,Mubashar Raza,Muhammad Shahid,Ezzeldin Ibrahim,Yanli Wang,Jiaoyu Wang,Chengqi Yan,Qianli An,Jason C. White,Bin Li
Rice blast disease (RBD) caused by Magnaporthe oryzae , threaten food security by cutting agricultural output. Nano agrochemicals are now perceived as sustainable, cost-effective alternatives to traditional pesticides. This study investigated bioformulation of moringa chitosan nanoparticles (M-CsNPs) and their mechanisms for suppressing RBD while minimizing toxic effects on the microenvironment. M-CsNPs, sized 46 nm with semi-spherical morphology, significantly suppressed pathogen growth, integrity, and colonization at 200 mg L −1 in vitro . Greenhouse tests with foliar exposure to the same concentration resulted in a substantial 77.7 % reduction in RBD, enhancing antioxidant enzyme activity and plant health. Furthermore, M-CsNPs improved photosynthesis, gas exchange, and the nutritional profile of diseased rice plants. RNA-seq analysis highlighted upregulated defense-related genes in treated rice plants. Metagenomic study showcased reshaping of the rice microbiome, reducing Magnaporthe abundance by 93.5 %. Both healthy and diseased rice plants showed increased microbial diversity, particularly favoring specific beneficial species Thiobacillus , Nitrospira , Nocardioides , and Sphingomicrobium in the rhizosphere and Azonexus , Agarivorans , and Bradyrhizobium in the phyllosphere. This comprehensive study unravels the diverse mechanisms by which M-CsNPs interact with plants and pathogens, curbing M. oryzae damage, promoting plant growth, and modulating the rice microbiome. It underscores the significant potential for effective plant disease management.
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FOOD CHEMISTRY
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2025
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IF 9.8
Hongxia Wang,Liang Xu,Shiyi Qiu,Juncheng Zhu,Shihao Qiao,Liang Ma,Yuhao Zhang
Gelatin/chitosan nanoparticles loaded with carminic acid were adopted to construct and stabilize high internal phase Pickering emulsions for 3D/4D printing. Carminic acid was incorporated into gelatin/chitosan nanoparticles with excellent compatibility via interactions like hydrogen bonding and bridging flocculation. These gelatin/chitosan/carminic acid complex nanoparticles exhibited outstanding emulsifying ability, due to the fact that nanoparticle's amphiphilicity (hydrophilic-lipophilic balance) and interfacial wettability (contact angle) promoted its adsorption at the oil-water interface. The optimal high internal phase Pickering emulsion with proper-content carminic acid illustrated uniform droplets, excellent emulsion appearance, high viscosity, great thixotropy, high yield stress, high mechanical strength and outstanding thermal stability. The optimal emulsions also presented excellent 3D/4D printability with high shape maintainability, as well as potential usage in dysphagia diets. This study provides important implications for stabilizing emulsions with carminic acid-loaded nanoparticles and constructing creative, high-quality and functional food products via 3D/4D printing.
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Food Packaging and Shelf Life
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2025
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IF 10.6
Yanyan Wang,Ruoran Qin,Jiayi Xue,Hui Li,Longwei Jiang
Succinate cyclodextrin/chitosan nanoparticles (SCC NPs) loaded with resveratrol (Res) were successfully synthesized by self-assembly. Nanocomposite films were prepared by adding R-SCC NPs to sodium alginate (SA). The structure, physical and chemical properties, and antioxidant, antibacterial and biodegradability properties of the nanocomposite films were characterized. Scanning electron microscopy (SEM) results revealed that R-SCC NPs in 1–6 % were uniformly distributed in the SA film matrix. FTIR analysis revealed that the R-SCC NPs formed intermolecular hydrogen bonds with SA. The maximum tensile strength of the nanocomposite film was 44.24 ± 0.82 MPa, which was 153.50 % of that of the SA film. The addition of R-SCC NPs markedly enhanced the UV absorption capacity, antioxidant stability, and antibacterial activity of the nanocomposite films. In addition, because Res was encapsulated in the SCC NPs, control over the release from the composite film was achieved. This film has excellent biodegradability in soil and can regulate the soil microbial community. Furthermore, the developed film package can effectively prolong the expiry date of blueberries, so the SA film containing R-SCC NPs as active packaging has significant potential for maintaining food quality.
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Journal of Biomaterials and Tissue Engineering
·
2017
·Vol. 7
·期 8
·605-613
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IF 1.383
Yang Bin,Gao Ge,Liu Lintao,Li Yang,Zhao Lihua,Xing Hua
In recent years, breast cancer attracts more and more attention because of its high mobility and high mortality for women. Phototherapy, including photothermal therapy (PTT) and photodynamic therapy (PDT), provides a new pathway to breast cancer therapy. Herein, we developed multifunctional
IR780 loaded nanoparticles based on folic acid modified chitosan (FASOC-IR780 NPs) for targeted imaging and phototherapy of cancer compatible with a single-nearinfrared (NIR) laser irradiation. In vitro characterizations demonstrated the self-assembled FASOC-IR780 NPs own narrow size
distribution and encouraging photothermal performance with enhanced generation of ROS under laser irradiation. The cytotoxicity studies on MDA-MB-231 cells showed enhanced toxicity upon NIR light irradiation. NIR small animal imaging demonstrated the significant tumor-selectivity of the nanoparticles
to breast cancer cells that over expressed folate receptor. In vivo treatments with FASOC-IR780 NPs resulted in effective tumor suppression. These results indicate that the multifunctional FASOC-IR780 NPs show notable targeting and theranostic potential in breast cancer therapy.
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International Journal of Nanomedicine
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2023
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IF 8
Chun-ge Zhang,Qiao-ling Zhu,Yi Zhou,Yang Liu,Wei-liang Chen,Zhi-Qiang Yuan,Shu-di Yang,Xiao-feng Zhou,Ai-jun Zhu,Xue-nong Zhang,Yong Jin
N-Succinyl-chitosan (NSC) was synthesized and NSC nanoparticles (NPs) with loaded osthole (Ost) (Ost/NSC-NPs) were prepared by emulsion solvent diffusion. Subsequently, low-density lipoprotein (LDL)-mediated NSC-NPs with loaded Ost (Ost/LDL-NSC-NPs) were obtained by coupling LDL with Ost/NSC-NPs through amide linkage. The average particle size of Ost/NSC-NPs was approximately 145 nm, the entrapment efficiency was 78.28%±2.06%, and the drug-loading amount was 18.09%±0.17%. The release of Ost from Ost/NSC-NPs in vitro showed a more evident sustained effect than the native material. The half maximal inhibitory concentration of Ost/LDL-NSC-NPs was only 16.23% that of the free Ost at 24 hours in HepG2 cells. Ost inhibited HepG2 cell proliferation by arresting cells in the synthesis phase of the cell cycle and by triggering apoptosis. Cellular uptake and subcellular localization in vitro and near-infrared fluorescence real-time imaging in vivo showed that Ost/LDL-NSC-NPs had high targeting efficacy. Therefore, LDL-NSC-NPs are a promising system for targeted Ost delivery to liver tumor.
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2026
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IF 6
Tao Wang,Yang Han,Jingyi Chen,Gaochao Zhou,Lixiao Cai,Tong Li,Xiaoxiao Guo,Jing Han
A nano-in-microneedle system delivers sulforaphane efficiently through the skin barrier for dermatitis treatment. • Network pharmacology predicts that sulforaphane treats irritant contact dermatitis by targeting multiple inflammatory pathways. • Chitosan nanoparticles encapsulate sulforaphane with high efficiency, protecting it from degradation and enabling sustained release. • The combined microneedle patch shows superior anti-inflammatory effects in mice, outperforming conventional dexamethasone ointment. • Methacrylated chitosan microneedles create microchannels, enhancing skin retention eleven-fold. Treatment of irritant contact dermatitis (ICD) is limited by the stratum corneum barrier, which hinders drug delivery and bioavailability. Sulforaphane (SFN) offers anti-inflammatory potential but suffers from poor stability and a short half-life. To address this, we developed a chitosan methacrylate (CSMA) hydrogel microneedle (MN) patch loaded with SFN-chitosan nanoparticles (SFN-CSNPs) for enhanced transdermal delivery.Network pharmacology identified 33 potential ICD-related targets of SFN, including SRC, EGFR, and JAK2, implicating pathways such as AGE-RAGE and Relaxin signaling. SFN-CSNPs were optimized via a Box-Behnken design, yielding nanoparticles with a size of 180–220 nm, PDI < 0.3, a Zeta potential of ∼ 24 mV, and a high encapsulation efficiency (91.94 ± 1.22%). These were incorporated into CSMA MNs using micromolding. The MN arrays exhibited robust mechanical strength (1.53 N/needle), enabling effective stratum corneum penetration. In vitro, the system achieved sustained release (183.6 ± 2.1 µg/cm 2 over 60 h) and 11-fold higher skin retention than the SFN solution. It also demonstrated excellent stability, retaining > 85% drug content after 30 days at 4 °C. In an ICD mouse model, SFN-CSNPs@CSMA MNs significantly reduced erythema, edema, and epidermal thickening (45.37 ± 2.86 µm), approaching normal levels (20.03 ± 3.20 µm), and outperformed both SFN-CSNPs solution and dexamethasone ointment. This nano-MN platform effectively overcomes skin barriers, enhances drug stability, and enables localized, sustained release, offering a promising strategy for delivering unstable natural compounds such as SFN to treat inflammatory skin diseases. Download: Download high-res image (210KB) Download: Download full-size image
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