International Journal of Electrochemical Science
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2023
·
IF 1.5
Zhiqiang Wei,Shaofang Guo,Liuyang Cheng,Ting Li,Yanli Zhang,Hui Yang
A sensitive electrochemical sensor for the simultaneous determination of acetaminophen (ACOP) and tyrosine (Tyr) was proposed based on MWCNTs-doped poly (glycine) (p-gly)/ poly (acrylic acid) (PAA) conducting polymers modified screen-printed electrode (SPE). The incorporation of MWCNTs and p- gly/ PAA composite conductive polymers brought about enhanced electro-catalytic activity and additional binding sites for ACOP and Tyr. Cyclic voltammetry (CV) and linear sweep voltammetry (LSV) were performed to investigate the electrochemical behaviors of ACOP and Tyr. Under the optimal experimental conditions, the oxidation peak currents of ACOP and Tyr increased linearly with two concentration intervals over the range of 0.25-120 μM and 0.4-150 μM, respectively. The detection limits (S/N = 3) were 0.08 μM for ACOP and 0.13 μM for Tyr. Moreover, the sensor has been successfully applied for the determination of ACOP and Tyr in human serum samples, showing its great application prospects in pharmaceutical analysis.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Yue Luo,Siyue Zhu,Jie Peng,Heping Cui,Qingrong Huang,Baojun Xu,Chi-Tang Ho
Amadori rearrangement products (ARPs), as intermediates of the Maillard reaction (MR), are potential natural flavor additives but there is a lack of investigation especially in oligopeptide-ARPs. This study for the first time conducted a systematic analysis in comparing ARPs of glycine, diglycine, triglycine, and glucose to corresponding classic MR systems, including production, stability, and flavor analysis. The ARPs were effectively produced by prelyophilization with heating at 70 °C for 60 min and purified to 96% by a two-step purification method. Correlated with the stability order of amino compounds (glycine > diglycine > triglycine), the stability order of ARPs was Gly-ARP > Digly-ARP ≈ Trigly-ARP. In a negative correlation with heating temperature and time, ARPs were less stable than original amino compounds at high temperatures (100, 130, and 160 °C). ARPs exhibited better flavor formation ability in pyrazines and furans than MR systems, with similar flavor compositions but different preferences. Diglycine- and triglycine-ARPs exhibited better flavor formation efficiency than glycine-ARP. Heating temperature and time, initial pH, and carbon chain length were found to be the parameters that affect the stability and flavor formation of ARPs. This study suggested that ARPs, especially peptide-ARPs, have great potential for usage as food flavor additives in the future.
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2019
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IF 2.442
Xiaoling Yang,Xue Liu,Qianqian Wu,Xu Lv,Bo Li,Hongzhe Sun,Wenfeng Xu,Xiaoling Liao
Due to their excellent biocompatibility and suitable elastic modulus, carbon–carbon composites have excellent prospects for a wide range of applications such as artificial joints and bone replacements. However, the hydrophobicity and poor bioactivity limit their application in bone repair and substitution. Herein, a two-step surface modification of carbon–carbon composites was proposed to overcome these disadvantages. A polydopamine (PDA) layer was first deposited on the surface of carbon–carbon composites, followed by covalent coupling with arginine–glycine–aspartic acid (RGD) in varying concentrations. The surface-modified materials were characterized using a variety of techniques including water contact angle, scanning electron microscopy, atomic force microscopy, and protein adsorption. In addition, the cellular proliferation, alkaline phosphatase activity, and real-time quantitative PCR (RT-PCR) analysis were further tested in vitro. The results indicated that polydopamine and RGD nanoparticles formed on the surface, which significantly enhanced the hydrophilicity following modification. The RGD–PDA-coated carbon–carbon composites exhibited excellent roughness, protein adsorption behavior, and uniform morphology when a concentration of 50 µg/mL RGD was used, unlike blank and polydopamine-modified carbon–carbon composites. The in vitro cell co-culture experiments demonstrated that proliferation and alkaline phosphatase expression in osteoblasts was greatly improved after RGD–PDA coating. The RT-PCR results revealed that the expression of differentiation markers (alkaline phosphatase and osteocalcin) in cells on the RGD–PDA-coated carbon–carbon composites was higher than that on the blank and polydopamine-modified carbon–carbon composites, indicating that the RGD–PDA coating could support cell proliferation and promote osteogenic differentiation of MC3T3-E1 cells. In particular, the cells cultured on this material grew linearly along the morphological features of the material, which was effective for the subsequent regulation of cell growth. Our study suggested that the RGD–PDA coating was an effective method of enhancing the hydrophobicity and the cellular bioactivity of carbon–carbon composites, thus extending applications of carbon–carbon composites to the bone repair field.
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MACROMOLECULAR BIOSCIENCE
·
2015
·Vol. 15
·期 6
·829-838
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IF 3.851
Haiyang Yu,Zhaohui Tang,Dawei Zhang,Wantong Song,Taicheng Duan,Jingkai Gu,Xuesi Chen
A novel random copolypeptide of ornithine, arginine, glycine, and aspartic acid [Poly(ornithine-co-arginine-co-glycine-co-aspartic acid), Poly(O,R,G,D)] has been prepared through ring-opening polymerization of N -δ-carbobenzoxy-l-ornithine N -carboxyanhydride [Orn(Cbz)-NCA)], l-glycine N -carboxyanhydride (Gly-NCA) and β-benzyl l-aspartate N -carboxyanhydride [Asp(Bn)-NCA], following by subsequent deprotection and guanidization. The structure of Poly(O,R,G,D) was confirmed by nuclear magnetic resonance (NMR) spectroscopy and gel permeation chromatography (GPC). Low cytotoxicity of Poly(O,R,G,D) was confirmed from MTT assay. The Poly(O,R,G,D) contain some internal sequences of RXXR ( X = O, R, G, or D) that could be proteolytically cleaved to expose the cryptic CendR element and bind to Neuropilin-1. This would lead to vascular and tissue permeabilization. Therefore trypsin-cleaved Poly(O,R,G,D) increase the vascular leakage of Evans blue from dermal microvessels at the injection site in vivo skin permeability assay. The intratumoral injection of the Poly(O,R,G,D) significantly enhanced the concentration of cisplatin-loaded nanoparticles in MCF-7 solid tumors. These results show that Poly(O,R,G,D) could increase the vascular leakage and tissue penetration of nanoparticles in a solid tumor and can be used as a potential polymeric tumor-penetrating agent.
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POLYMER DEGRADATION AND STABILITY
·
2024
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IF 6.3
Jinke Yu,Ling Sun,Lan Ding,Yufei Cao,Xiaohui Liu,Yuanlin Ren,Yuesheng Li
When confronted with a fire, the flammability of rigid polyurethane foam (RPUF) makes it extremely easy to burn violently. In this work, a green biomass derived flame retardant coating was synthesized with phytic acid as acid source, D-sorbitol as carbon source and glycine as gas source. A simple UV curing technique was used to improve the flame retardancy of RPUF . Coated RPUF exhibited a limiting oxygen index value of up to 39.7 % and successfully met UL-94 testing criteria with V-0 rating. In comparison to control RPUF, coated RPUF showed an extended ignition time of 3 s, a remarkable reduction of approximately 60 % in the peak heat release rate, and possessed commendable smoke suppression property. The flame retardant mechanism was analyzed, unveiling the emergence of compact char residue on the surface of coated RPUF after burning, accompanied by the liberation of non-combustible gases. Therefore, these results demonstrated that cost-effective UV-curable flame retardant coating forms protective char layer on the surface of RPUF, providing efficient flame retardancy while preserving its excellent properties.
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Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Yuhui Xue,Changyan Guo,Jingjing Wang,Xueli Yang,Meng Wang,Jide Wang
The overuse of antibiotics poses a serious threat to the ecosystem. In particular, antibiotic residues in the aquatic environment are particularly serious. Therefore, preparation of efficient adsorbents for removing trace antibiotic residues from the environment becomes essential. In this work, a series of magnetic porous carbon material (Fe-MPC-gly) derived from MIL-101, which was regulated by glycine, were prepared by high temperature calcination for the adsorption of trace sulfonamide antibiotics (SAs) in water. The morphology of MIL-101 was found to gradually change from a regular ortho-octahedron to an acicular spindle with the increase as the glycine content increased. The results of adsorption experiments showed that Fe-MPC-gly-2 had the best adsorption effect on SAs. In particular, the adsorption of Fe-MPC-gly-2 on SAs could reach the adsorption equilibrium in 5 min, and the maximum adsorption amounts were 50.0 mg·g −1 and 38.2 mg·g −1 for sulfameter and sulfachloropyridazine, respectively. The adsorption kinetic model was best fitted to the quasi-first-order kinetic model. The possible adsorption mechanisms were pore filling, hydrogen bonding and π-π interactions. Meanwhile, the synthetic strategy of this study provides an idea for the preparation of efficient adsorbents for antibiotics.
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JOURNAL OF FLUORESCENCE
·
2023
·Vol. 33
·期 4
·1505-1513
·
IF 2.7
Wu Sifei,Fang Linxia,Li Yihan,Wang Hai-Bo,Zhang Hongding
Changes in Sn 2+ and glycine levels are relevant to many important physiological procedures in human health. However, investigation of their physiological functions is limited because few versatile methods towards Sn 2+ and glycine detection have been developed. In this work, a fluorescence turn on–off-on strategy was firstly constructed for rapid and sensitive detection of Sn 2+ and glycine through the specific binding between Sn 2+ and glycine. Carbon nanodots (CDs) with a quantum yield of 19.5% were synthesized by utilizing inner film of waste eggshell as carbon source and employed as fluorescent probe. In the presence of Sn 2+ , the fluorescence of CDs was quenched by Sn 2+ via the primary inner filter effect (IFE). However, the binding between Sn 2+ and glycine prevented the IFE between Sn 2+ and CDs, resulting in fluorescence recovery of CDs. Under optimized conditions, the fluorescent response of CDs displayed good linear relationships with the concentrations of Sn 2+ in the range of 10–200 µM and 200–5000 µM, and the limit of detection (LOD) was 2.4 µM. For glycine detection, a good linear relationship was obtained in the concentration range of 5–1000 µM with a low LOD down to 0.76 µM. Moreover, the practicability of the assay was also demonstrated by measuring glycine content in human serum samples. This work provides an economical, green and fast method for biological analysis of Sn 2+ and glycine.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Shangjie Wei,Heping Cui,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
The browning inhibition of cysteine on the Maillard reaction of glycine-xylose performed under stepwise increased temperature was investigated. The browning degrees of the final products prepared with cysteine addition at different time points were found dissimilar, and the addition time point of cysteine yielding the lightest browning products was consistent with the time when the glycine-xylose Amadori rearrangement product (GX-ARP) reached its maximum yield. To clarify the reason for browning inhibition caused by cysteine, the evolution of key browning precursors formed in the GX-ARP model with cysteine involved was investigated by HPLC with a diode array detector. The results on the browning degree of the thermal reaction products of GX-ARP with cysteine addition showed great inhibition of α-dicarbonyl generation, which resulted in a significant increase in the activation energy of GX-ARP conversion to browning formation during heat treatment. Strong evidence suggested that the additional cysteine got involved in GX-ARP degradation and reacted with the deoxyosones derived from GX-ARP to yield cyclic 2-threityl-thiazolidine-4-carboxylic acid (TTCA). TTCA formation shunted the degradation of deoxyosones into short-chain α-dicarbonyls, which were important browning precursors, and consequently inhibited the Maillard browning.
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NUCLEAR MEDICINE AND BIOLOGY
·
2022
·
IF 2.947
Hongliang Wang,Liu Yang,Keyi Lu,Xiaoshan Guo,Rui Xi,Weixuan Dong,Kaixin Qin,Haiyan Liu,Hua Wei,Yan Cheng,Zhifang Wu,Sijin Li
Objective Given the limitation of biomarkers to predict the renal function progression in diabetic nephropathy , N-(6-[ 18 F]Fluoropyridin-3-yl)glycine (6-[ 18 F]FPyGly) was used to evaluate renal function progression in a rat model of diabetic nephropathy. Methods Twenty male Sprague-Dawley rats were randomly divided into four groups, including the healthy control group (HC group), diabetic nephropathy group (DNM group), routine diet treated diabetic nephropathy group (RDNM group), and high fat/high sucrose -diet-fed diabetic nephropathy group (HDNM group). All renal function parameters were determined from animal PET renograms . P and T max represent the curve peak counts and the time to the curve peak counts of 6-[ 18 F]FPyGly in kidneys after injection, C 1/2 and the 15 min/Peak ratio represent the time from peak to 1/2 peak in the clearance phase, and the ratio of the curve counts at 15 min to the curve peak counts. Results P, T max , C 1/2 , and 15 min/peak ratio of each rat were significantly correlated with S-Cr, BUN . There were significant differences in T max , P, serum creatinine (S Cr), and blood urea nitrogen (BUN) levels between HC and DNM groups. P and the 15 min/Peak ratio were significantly different among DNM, RDNM, and HDNM groups, while T max and C 1/2 were only significantly different between DNM and RDNM or HDNM groups. There only was a significant difference in BUN between the DNM and HDNM groups. Conclusion The renal function parameters P, T max , C 1/2 and 15 min/peak value obtained by dynamic renal imaging based on 6-[ 18 F]FPyGly could reflect changes of renal function in rats, which had a good correlation with S Cr and BUN, and showed more efficient in the diagnosis of diabetic nephropathy and renal function classification than S Cr and BUN.
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BIOCHEMICAL PHARMACOLOGY
·
2022
·
IF 6.1
Yanbei Tu,Lihua Tan,Tao Lu,Kai Wang,Haiyong Wang,Bing Han,Yuxin Zhao,Hanbin Chen,Yanfang Li,Haixia Chen,Meiwan Chen,Chengwei He
The roots of Glycine tabacina are used to treat rheumatoid arthritis (RA) and joint infection in folk medicine. Glytabastan B (GlyB), a newly reported coumestan isolated from this species, was found to significantly attenuate IL-1β-induced inflammation in SW982 human synovial cells at 3 and 6 μM, as evidenced by the decreased levels of pro-inflammatory mediators and matrix metalloproteinases (MMPs). GlyB also suppressed RANKL-induced osteoclastogenesis, decreased the expression of osteoclastogenic markers (NFATc1, CTSK , MMP-9) and osteoclast-mediated bone resorption. Further, GlyB administration (12.5 and 25 mg/kg) significantly inhibited inflammation, osteoclast formation and disease progression in collagen-induced arthritis (CIA) mice. Integration of network pharmacology , quantitative phosphoproteomic and experimental pharmacology results revealed that these beneficial actions were closely associated with the blockade of GlyB on the activation of MAPK , PI3K/AKT and their downstream signals including NF-κB and GSK3β/NFATc1. Drug affinity responsive target stability (DARTS) assay, cellular thermal shift (CETSA) assay and molecular docking analysis confirmed that there were direct interactions between GlyB and its target proteins ERK2 , JNK1 and class Ⅰ PI3K catalytic subunit p110 (α, β, δ and γ), which significantly contributed to the inhibition of activation of MAPK and PI3K/AKT pathways. In conclusion, these results strongly suggest GlyB is a promising multiple-target candidate for the development of agents for the prevention and treatment of RA.
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AQUACULTURE
·
2013
·
IF 2.009
Shi-wei Xie,Li-xia Tian,Yan Jin,Hui-jun Yang,Gui-ying Liang,Yong-jian Liu
An 8-week feeding trial was conducted to evaluate the optimum dietary glycine level in low fishmeal diet for juvenile Pacific white shrimp , Litopenaeus vannamei . Six practical diets (44.4% protein, 8.8% lipid) were formulated to contain graded levels (2.26, 2.33, 2.44, 2.58, 2.67 and 2.74%) of l -glycine. Each diet was randomly assigned to triplicate groups of 30 shrimps (approximately 0.61 g) and the shrimps were fed 4 times a day to apparent satiation. The results indicated that the weight gain and specific growth rate were significantly increased with the supplementation of glycine. The highest weight gain and specific growth rate were observed at 2.56% dietary glycine, and then decrease with a further increase in dietary glycine. Hepatosomatic index , protein productive value and condition factor were also significantly influenced by the dietary glycine levels and show a similar trend to those of growth performance. There were no significant differences observed in feed efficiency , protein efficiency ratio and survival among all treatments. Mg, Ca and Fe concentrations in whole body were significantly increased with the supplementation of glycine. Non-essential amino acid, essential amino acid and arginine ratio in muscle were significantly affected by the dietary glycine levels. Dietary glycine levels higher than 2.44% significantly increased the survival of shrimps after 2 h and 3 h of acute salinity change, and T-SOD activity and Na + /K + -ATPase activity in hematological and hepatopancreas before and after acute salinity change are also significantly affected by the dietary glycine levels. Analysis by second-order regression of weight gain indicated that the optimal dietary glycine level was 2.54% of the dry diet (5.7% of the dietary protein and 6.66% of the dietary amino acids).
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FLUID PHASE EQUILIBRIA
·
2016
·
IF 1.846
Hua Sun,Lu Wang,Baoshu Liu
The solubility of α-glycine in water with different additives (sodium nitrate, ammonium nitrate , ammonium sulfate , and ammonium chloride) was measured under atmospheric pressure across the temperature range of (293.15–343.15) K at intervals of 10.00 K using a dynamic laser method. Solubility was found to increase with increasing temperature and additive level. The experimental data were correlated with the modified Apelblat model, nonrandom two-liquid model (NRTL), and λh model. The R 2 of the fitting regression indicated good applicability of these models to the glycine solubility data in the studied systems ( R 2 > 0.99). Apparent standard enthalpy, entropy, and Gibbs free energy changes of the solution were calculated based on the van't Hoff equation to elucidate the dissolution mechanism. The results showed that the dissolving process of glycine was endothermic, and the main dissolution driving force contributor was enthalpy in all the additive + water systems studied. By means of enthalpy-entropy compensation analysis, a non linear Δ H sol Θ versus Δ G sol Θ enthalpy-entropy compensation plot with positive and negative slopes was obtained for the systems studied. The evaluated values of H E showed that the mixing process of α-glycine in all the systems studied was exothermic.
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