Nanoscale
·
2015
·Vol. 8
·期 4
·2205-2211
·
IF 7.394
Xiaohua Zhu,Tingbi Zhao,Zhou Nie,Zhuang Miao,Yang Liu,Shouzhuo Yao
In this work, nitrogen-doped carbon nanoparticle (N-CNP) modulated turn-on fluorescent probes were developed for rapid and selective detection of histidine. The as synthesized N-CNPs exhibited high fluorescence quantum yield and excellent biocompatibility. The fluorescence of N-CNPs can be quenched selectively by Cu(II) ions with high efficiency, and restored by the addition of histidine owing to the competitive binding of Cu(II) ions and histidine that removes Cu(II) ions from the surface of the N-CNPs. Under the optimal conditions, a linear relationship between the increased fluorescence intensity of N-CNP/Cu(II) ion conjugates and the concentration of histidine was established in the range from 0.5 to 60 μM. The detection limit was as low as 150 nM (signal-to-noise ratio of 3). In addition, the as-prepared N-CNP/Cu(II) ion nanoprobes showed excellent biocompatibility and were applied for a histidine imaging assay in living cells, which presented great potential in the bio-labeling assay and clinical diagnostic applications.
研究领域:
分析化学
·
生物医学
·
材料科学
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ANALYTICAL SCIENCES
·
2022
·Vol. 38
·期 1
·105-112
·
IF 1.967
Wu Xiaoxiao,Huang Jingjing,Zhang Chi,Kang Xuejun
A novel nanofiber-based solid phase extraction (NFSPE) coupled with HPLC–DAD was developed for the determination of salivary histidine, a new potential index of physiological events. The treatment of the sample was based on a coupling reaction of histidine with the diazotization products of sulfanilic acid and sodium nitrite in a slightly alkaline medium, followed by NFSPE. Nanofibers comprising polystyrene and zinc acetate were used as a novel solid-phase sorbent to extract derived histidine via the chelation of Zn 2+ with the imidazole group in the analyte. The eluted target compound was analyzed by HPLC–DAD. Good linearity was achieved with the concentrations of histidine ranging from 0.645 to 64.5 µM; the detection limit was 0.193 µM. The intra-day and inter-day coefficients of variation were less than 8.0%. The method was successfully applied to the determination of salivary histidine in the subjects exposed to a public speaking stress. Graphical abstract
研究领域:
分析化学
·
生物医学
·
材料科学
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2023
·
IF 2.6
Xia Li,Weiping Liu,Yahui Yin,Yu Zhou,Hongfei Xiang,Xianzhen Xu
Vapor–liquid equilibrium (VLE) is essential for separation processes, such as distillation and absorption in chemical production. In this work, the VLE of glycine, l-histidine, and l-tryptophan aqueous solutions was measured at pressures ranging from 5.3 to 101.1 kPa. Xu’s model was used, and the model was based on the nonrandom two-liquid (NRTL) model, which has been successfully correlated with VLE data at temperatures between 298.15 and 373.85 K, and the model parameters were obtained. In addition, the absorption peaks of three amino acid aqueous solutions were analyzed by Fourier transform infrared (FTIR) spectroscopy from a microscopic perspective. The results showed that the higher the solubility of amino acids in water, the stronger the hydrogen-bonding interaction with water. Furthermore, the total mean interaction energy of water in the systems was studied by using a COSMOthermX 2021 tool. As inferred from the calculations, the total mean interaction energy was slightly affected by the concentration. The structure–activity relationship was established between the microscopic interactions and the macroscopic VLE.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Wenmin Wu,Qixing Jiang,Pei Gao,Dawei Yu,Peipei Yu,Wenshui Xia
The effects of the L-hisdine (L-His)-assisted ultrasound on physicochemical characteristics and conformation of myofibrillar protein (MP) under reduced-salt condition were investigated using spectroscopic analysis , and the binding mechanism between L-His and MP was further elucidated through molecular docking and molecular dynamics (MD) simulations. UV second derivative spectra and intrinsic Try fluorescence spectra revealed that L-His formed a complex with MP and altered the microenvironment of MP. After L-His-assisted ultrasound treatment, MP showed smaller particle size, higher solubility, and more uniform atomic force microscopy image due to the decrease of α -helix content and the subsequent increase in zeta potential , active sulfhydryl content, and surface hydrophobicity . Molecular docking and MD simulations demonstrated the optimal docking pose (minimum binding affinity of −6.78 kcal/mol) and revealed hydrophobic interactions and hydrogen bonds as the main interaction forces between L-His and MP, with several residues (ILE-464, ILE-480, THR-483, ASN-484, GLY-466, ASP-463, PHE-246) identified as binding sites.
研究领域:
食品科学
·
生物化学
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Advanced Science
·
2026
·
IF 14.1
Kaiyuan Jiang,Fen Xiong,Yiwen Peng,Lingfei Meng,Xinran Wang,Yingruo Xu,Tian Tang,Hongchang Gao
Diabetic cardiomyopathy (DCM) is a major cardiovascular complication of diabetes with limited effective interventions. Using a streptozotocin-induced insulin-deficient, type 1 diabetes-like DCM mouse model, we show that intermittent fasting (IF) improves cardiac function and attenuates myocardial remodeling. Antibiotic-mediated microbiota depletion largely abolished these benefits, whereas fecal microbiota transplantation from IF-treated donors recapitulated cardioprotection, supporting a causal role of the gut microbiota. Metagenomic profiling identified Akkermansia muciniphila ( A. muciniphila ) as a prominent IF-responsive taxon, and A. muciniphila supplementation alleviated cardiac injury without obvious improvement in glycaemia. Integrated serum and heart metabolomics identified 1-methyl-L-histidine as a microbiota-associated metabolite reduced in diabetes but restored by IF and A. muciniphila . In vitro and ex vivo assays further supported an L-anserine-linked microbial route for 1-methyl-L-histidine generation. Importantly, oral 1-methyl-L-histidine supplementation recapitulated key cardioprotective effects, remodeled cardiac lipid homeostasis, and reduced lipid peroxidation and oxidative injury. Together, these findings support a gut microbiota–metabolite–lipid axis associated with IF-related cardioprotection in DCM and highlight microbial metabolites as tractable targets to complement dietary intervention.
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MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 8
·563
·
IF 5.4
Liu Huan,Chen Yuhan,Sun Han,Wang Boyan,Liu Aihua
The sensitive monitoring of bisphenol A (BPA) concentration remains a critical challenge in environmental analysis and public healthcare. In this study, Mn 3 O 4 nanorods were facilely prepared in an aqueous solution at room temperature using Mn 2+ as the precursor, NaOH as the basic source and L-histidine as the mediator. The as-prepared Mn 3 O 4 nanorods exhibit excellent laccase-mimicking activity with a catalytic efficiency of 3.85 × 10 − 3 min − 1 . The catalytic mechanism was confirmed to be oxygen vacancies by electron paramagnetic resonance spectrum and enzymatic inhibition experiment. Taking advantage of the excellent laccase-mimetic properties of the Mn 3 O 4 nanozyme, a sensitive and selective colorimetric sensing platform for BPA detection was constructed. The colorimetric sensing platform achieved a linear detection range of 1–60 µM BPA and a detection limit of 0.23 µM for BPA (3σ/S). The proposed method was applicable to the determination of BPA in plastic-packaged white vinegar, bottled water and milk with good recoveries and reproducibility. This work not only provides a novel and efficient analytical tool for the rapid detection of BPA but also offers a new insight into the design and synthesis of other amino acid-mediated nanozymes for environmental monitoring applications. Graphical
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Shi-Ting Zhang,Panpan Li,Caiyun Liao,Tingting Luo,Xingming Kou,Dan Xiao
A ruthenium(II) bipyridyl complex conjugated with functionalized Schiff base (RuA) has been synthesized and functioned as a luminescent probe. The luminescence of RuA was greatly quenched by Cu 2+ due to its molecular coordination with paramagnetic Cu 2+ . Subsequently, the addition of l -Histidine can turn on the luminescence of the RuA-Cu(II) ensemble, which can be attributed to the replacement of RuA in RuA-Cu(II) ensemble by l -Histidine. On the basis of the quenching and recovery of the luminescence of RuA, we proposed a rapid and highly sensitive on-off-on luminescent assay for sensing Cu 2+ and l -Histidine in aqueous solution. Under the optimal conditions, Cu 2+ and l -Histidine can be detected in the concentration range of 5 nM–9.0 μM and 50 nM–30 μM, respectively, and the corresponding detection limits were calculated to be 0.35 and 0.44 nM (S/N=3), separately. The proposed luminescent probe has been successfully utilized for the analysis of Cu 2+ and l -Histidine in real samples (drinking water and biological fluids). Furthermore, the probe revealed good photostability, low cytotoxicity and excellent permeability, making it a suitable candidate for cell imaging and labeling in vitro.
研究领域:
分析化学
·
生物化学
·
医学检测
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NANOTECHNOLOGY
·
2025
·Vol. 36
·期 38
·385302
·
IF 2.8
Zhongqiang Min,Qifeng Chen,Xuchao Chen
In this study, L-His-N-carbon quantum dots (CQDs) were synthesized via a microwave-assisted method using homemade hardwood cellulose with a dryness of 20% as the carbon source and L-histidine as the nitrogen source. The results demonstrated that when the mass ratio of cellulose to L-histidine was 1:5 and the reaction time was 40 min, the L-His-N-CQDs exhibited the highest fluorescence quantum yield of 22.43%. A series of characterizations revealed their superior fluorescence performance and uniform size distribution. Subsequently, L-His-N-CQDs were incorporated into a plant oil-based binder to formulate fluorescent anti-counterfeiting ink. Adjusting the mixing ratio of tung oil and soybean oil effectively regulated the ink’s drying properties. When the tung oil–soybean oil ratio was 2:1 or 3:1, the ink achieved optimal drying properties, transparency, and fluidity, ensuring excellent printability and anti-counterfeiting performance in screen printing. Fluorescence spectroscopy tests indicated that the composite fluorescent anti-counterfeiting ink possessed remarkable extended fluorescence stability and anti-quenching properties. The ink’s resin binder, plant oil solvent, and additives did not hinder the luminescence of carbon quantum dots. Thus, the prepared ink is highly applicable in anti-counterfeiting printing technologies and related fields, offering new material options for such printing techniques.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Qibin Yan,Yunfan Li,Jiaheng Zhao,Zhiwang Yang,Ziqiang Lei
Conventional curing agents typically possess a single property and the challenge of inadequate interfacial adhesion between inorganic flame-retardant additives and the epoxy resin (EP). This study presents a multifunctional flame retardant curing agent (HFP-MM) synthesized by functionalizing magnesium hydroxide (MH) with L-histidine (HIS), phosphite, and melamine (MEL). This curing agent was compounded with varying amounts (wt%) of Ammonium polyphosphate (APP) and added to EP to establish an intumescent flame retardant system. The results indicate that EP formulation containing 10 wt% HFP-MM and 10 wt% APP achieved UL-94 V-0 level and exhibited the limiting oxygen index (LOI) measurement reaching 29.6 %. Furthermore, compared to the flame retardant free of EP, the peak heat release rate, peak CO production rate and total smoke production were dropped by 88.9 %, 86.0 % and 74.8 %. Regarding flexural performance, the flexural modulus and flexural strength increased by 129.6 % and 137.4 %, respectively. The functionalized MH flame-retardant curing agent prepared in this study, when applied to EP, exhibits excellent flame-retardant and mechanical properties, proving effective in reducing the fire hazard of EP materials.
研究领域:
高分子化学
·
材料科学
·
阻燃材料
·
复合材料
·
界面改性
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热稳定性
·
燃烧性能
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高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Faisal Ahmad,Yelan Xiao,Tong Shu,Guangyao Zhang,Xueji Zhang
Electrochemiluminescence (ECL) is rapidly emerging as an excellent electrochemical analytical technique for the specific and sensitive detection of various biomarkers and hazardous trace metals. Among ECL emitters, gold nanoclusters (AuNCs) have proven to be excellent luminophores due to their remarkable luminescent properties, stability, and biocompatibility. However, the low ECL efficiency of AuNCs precludes their application in ultrasensitive biosensing. One of the key reasons for the low ECL efficiency of AuNCs is the nonradiative energy transitions arising from intramolecular vibrations and rotations of the ligands on the surface of AuNCs. Herein, we explore the ECL of Zn2+/Au22(SG)18/PDA nanoclusters (NCs), where Zn2+ and 2,6-pyridinedicarboxaldehyde (PDA) activate the restriction of intramolecular motion (RIM) through aggregation-induced emission (AIE) to aggregation-induced enhanced emission (AIEE). This effect is achieved via cross-linking of Zn2+ and PDA with glutathione on the surface of Au22(SG)18, resulting in a significant enhancement of ECL emission compared to Au22(SG)18 NCs alone. A sensitive ECL sensing system was devised with Zn2+/Au22(SG)18/PDA as the ECL emitter, with triethylamine (TEA) as the coreactant, for the detection of Cu2+ and histidine in the linear ranges of 0.1 to 40 μM and 1 to 85 μM, respectively, with detection limits of 0.705 and 1.928 μM, respectively.
研究领域:
分析化学
·
纳米技术
·
电化学
·
生物传感
·
环境监测
·
材料科学
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高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 35
·e02990
·
IF 2
Yijun Yu,Haibiao Chen,Chen Zhang,Jie He,Linling Li,Chao Teng
In this work, a good water-dispersible GO-Cu 2+ -1, 8-diaminonaphthalene complex (GCU) was synthesized from graphene oxide (GO), CuCl 2 , and 1, 8-diaminonaphthalene in mild condition. The GCU achieved histidine detection with high selectivity and high sensitivity. Upon addition of histidine, the fluorescence intensity of GCU at 410 nm increased significantly due to the blocked photoinduced electron transfer (PET) process. The linear concentrations range of histidine detection was from 0.1 to 4.0 µM, with a limit of detection (LOD) of 0.027 µM. Common amino acids and proteins do not affect the detection of histidine by GCU in water. Furthermore, the GCU has been successfully applied not only to detect histidine in water but also to measure histidine in milk. The fluorescence sensor-based assay using GCU represents a promising method for nutritional monitoring. Graphical A highly water-dispersible chemosensor GCU was synthesized from GO, CuCl 2 , and 1,8-diaminonaphthalene. The nanostructured ternary complex exhibits weak fluorescence at 410 nm due to the PET mechanism. It responds to histidine in aqueous solutions with a limit of detection (LOD) of 0.027 µM. Additionally, it can detect histidine in real milk samples, exhibiting promising application potential.
研究领域:
分析化学
·
生物传感
·
纳米材料
·
食品分析
·
分子识别
·
荧光检测
·
营养监测
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TALANTA
·
2012
·
IF 3.794
Zhuo Du,Suling Zhang,Chanyuan Zhou,Miao Liu,Gongke Li
In this work, the multi-walled carbon nanotubes were covalently functionalized with l -histidine (His–MWNTs) as online pseudospecific affinity adsorbent for immunoglobulin G (IgG) separation and purification with a simple surface modification method, using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimde (NHS). The affinity of the His–MWNTs toward IgG was investigated in a microcolumn incorporated into a sequential injection system, which also involves an UV spectrometer with a flow cell for online real-time detection. The incorporation of histidine as affinity groups noticeably increased the selectivity and binding capacity of MWNTs for IgG and the His–MWNTs exhibited high retention and recovery rate of nearly 100% under optimized conditions. This separation and enrichment process made it possible to determine a lower concentration range of IgG in serum from 1.0–33 μg/mL with a detection limit of 0.3 μg/mL with a sampling volume of 4.0 mL. The static and dynamic adsorption capacities obtained were 267 mg of IgG/g His–MWNTs and 35 mg/g in aqueous solution, respectively, which are among the highest reported results in literatures employing affinity separation methods. Desorption of IgG from His–MWNTs could be accomplished by lowering the pH to 1.5 with glycine–HCl buffer. The practical application of His–MWNTs for separation of IgG in serum was evaluated with sodium dodecyl sulfate-polyacrylamide gel electrophoresis which confirmed that the purity of recovered IgG from human serum was over 85% and better than a commercial product.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
生物医学工程
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高影响因子
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