LIFE SCIENCES
·
2020
·
IF 3.647
Yaoxiang Zhu,Ruijue Liu,Zhenglin Shen,Guoqiang Cai
Aim Luteolin and lycopene are common natural products, widely existing in nature, and both of which were reported to have various biological functions including anti-inflammatory, anti-obesity and anti-NAFLD. In the present study, we aimed to evaluate the therapeutic efficacy of luteolin and lycopene in combination and its latent molecular mechanisms in vitro and in vivo models of NAFLD. Main methods Sodium palmitate (PA)-induced steatotic HepG2 cells and primary hepatocytes, and high-fat diet-induced C57BL/6J obese mice were treated with luteolin, lycopene and their combination. Metabolic parameters were measured. Key findings We found that luteolin (20 μM) + lycopene (10 μM) was the best therapeutic combination in PA-induced HepG2 cells, and significantly improve cell viability and lipid accumulation in PA-induced HepG2 cells and primary hepatocytes. In addition, luteolin (20 mg/kg) + lycopene (20 mg/kg) could ameliorate increased body weight and hepatocyte steatosis; regulate serum triglycerides, serum total cholesterol, hepatic triglycerides and hepatic total cholesterol; decrease serum alanine transaminase and aspartate transaminase. Furthermore, in vivo and in vitro, luteolin, lycopene and their combination had no effect on Sirt1 expression, but all of them could upregulate the expression of NAMPT, which could increase the level of NAD+, the co-substrate of Sirt1, indirectly activating Sirt1/AMPK pathway, and then inhibited lipogenesis and increased β-oxidation, defensing the “first hit”; they also inactivated nuclear factor-κB (NF-κB) and decreased the levels of IL-6, IL-1β and TNF-α, defensing the “second hit”. Significance Thus, luteolin and lycopene in combination can effectively ameliorate “two-hit” in NAFLD through activation of the Sirt1/AMPK pathway.
研究领域:
药理学
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分子生物学
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细胞生物学
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代谢
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营养学
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DOI
PubMed
高影响因子
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
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2021
·
IF 2.73
Jinhui Dong,Ou Xu,Jianxing Wang,Chunguang Shan,Xiumin Ren
Objective Luteolin has an anti-allergic effect but its mechanism is not clear. This study attempted to determine the mechanisms of luteolin in rhinitis.Methods Allergic rhinitis rat model was established by ovalbumin (OVA) stimulation. Then, the rats were treated with normal saline, luteolin, or lipopolysaccharide (LPS) for 14 days. Nasal symptoms were scored; the histopathological changes of nasal mucosa were detected by hematoxylin–eosin staining. Serum levels of Th1 type cytokines (IFN-γ, IL-2), Th2 type cytokines (IL-4, IL-5, IL-13), and OVA-specific IgE (sIgE) were determined by ELISA. The expressions of Toll-like receptor 4 (TLR4) and p65 in nasal mucosa were detected by Western blot or immunohistochemistry.Results Luteolin decreased symptom scores, specifically, the scores in control group, model group, model + 0.1 mg/kg luteolin, model + 1 mg/kg luteolin, and model + 10 mg/kg luteolin groups were 0.63 ± 0.52, 7.88 ± 0.83, 1.38 ± 0.52, 2.75 ± 0.46, and 5.00 ± 0.53, respectively. Luteolin ameliorated nasal mucosa inflammation by promoting the down-regulated levels of Th1 type cytokines, and suppressing the up-regulated levels of Th2 type cytokines, OVE-sIgE, TLR4, and p65. LPS further increased symptom scores, aggravated nasal mucosa inflammation, improved the unbalance of Th1/Th2 type cytokines, and lowered the expressions of OVE-sIgE, TLR4, and p65. Moreover, LPS reversed the effect of luteolin on allergic rhinitis rats.Conclusion Luteolin ameliorated inflammation and Th1/Th2 imbalance via regulating the TLR4/NF-κB pathway in allergic rhinitis rats. This study provided novel evidence that luteolin could be used as a candidate drug in allergic rhinitis treatment.
研究领域:
免疫学
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药理学
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过敏
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
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2013
·Vol. 18
·期 1
·269-278
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IF 2.279
Lu Daban,Lin Shaoxiong,Wang Letao,Li Tong,Wang Chunming,Zhang Yan
Poly(diallyldimethylammonium chloride) (PDDA)-functionalized graphene sheets-multiwalled carbon nanotubes (G-CNTs) hybrid materials (PDDA-G-CNTs) were successfully synthesized. The materials were characterized by transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) spectra. Then, an electrochemical sensor based on PDDA-G-CNTs/β-cyclodextrin (PDDA-G-CNTs/β-CD) was fabricated for the sensitive determination of luteolin. In 0.2 M HAc–NaAc buffer solution (pH 5.0), the redox peak currents of luteolin increased significantly on PDDA-G-CNTs/β-CD modified glassy carbon electrode (GCE), suggesting that the composite film not only showed excellent electronic properties of PDDA-G-CNTs but also exhibited high molecular recognition capability of β-CD. The chronocoulometry investigation demonstrated that the composite could effectively increase the electrochemical active surface. Under the optimal conditions, the oxidation peak current of luteolin increased linearly with increasing the concentration in the range from 0.05 to 60 μM with the detection limit of 0.02 μM ( S / N = 3). The developed electrochemical sensor exhibited high selectivity, good stability and reproducibility, and also successfully determined luteolin in drug samples.
研究领域:
分析化学
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材料科学
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药物分析
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DOI
高影响因子
TALANTA
·
2014
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IF 3.511
Yang Wang,Yichun Wu,Huali Ge,Huanhuan Chen,Guiqin Ye,Xiaoya Hu
A novel solid-phase extraction sorbent , metal-organic frameworks and graphite oxide hybrid composite, was prepared by a solvothermal technique. The morphology and properties of the resultant material were examined by Fourier transform infrared spectroscopy, X-ray diffraction and field emission scanning electron microscopy . To evaluate the extraction performance of the resultant sorbent, luteolin was chosen as a model analyte. The extraction conditions were optimized. Based on these, a convenient and efficient solid-phase extraction procedure for the determination of luteolin was established and the subsequent quantification step was performed by square wave anodic stripping voltammetry . Under the optimal conditions, the oxidation current increased linearly with increasing the concentration of luteolin in the range of 5.0×10 −9 –5.0×10 −7 mol L −1 with a correlation coefficient of 0.9983 and a detection limit of 7.9×10 −10 mol L −1 . The relative standard deviation of seven successive scans was 4.20% for 5.0×10 −8 mol L −1 luteolin. This work not only proposes a useful method for sample pretreatment, but also reveals the great potential of metal-organic frameworks based hybrid materials as an excellent sorbent in solid-phase extraction.
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Zixuan Wang,Juan Peng,Houlin Li,Ganghui Chu
Luteolin is a variety of bioactive flavonoids, it is of great significance to quickly and accurately determine the content of luteolin. This paper established a simple and rapid ratiometric electrochemical sensor for the sensitive determination of luteolin. Nano-flowering zirconium-based metal-organic frameworks (UiO-66) were synthesized and silver nanoparticles (AgNPs) were directly grown on the surface of UiO-66 to prepare AgNPs@UiO-66 composites. Then, AgNPs@UiO-66 was drop-coated on the electrode surface to prepare the ratiometric electrochemical sensor. AgNPs@UiO-66 has the dual function of providing a stable internal reference signal and enhancing the electrochemical signal. Under optimal conditions, the peak current of luteolin (I Luteolin ) increased with increasing luteolin concentration, while the peak current of AgNPs@UiO-66 (I AgNPs@UiO-66 ), which is used as an internal reference signal, remained relatively stable. I Luteolin /I AgNPs@UiO-66 showed a good linear relationship in the range of 0.07 - 0.9 μM and 0.9 - 40 μM, and the limit of detection (LOD) was 0.017 μM (S/ N = 3). The sensor detected luteolin in peanut shells with recoveries ranging from 96.7 % to 107 %, which agreed with ultraviolet-visible spectroscopy. These results demonstrate the possibility of this ratiometric electrochemical sensor for detecting luteolin in real life.
研究领域:
分析化学
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植物学
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生物医学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2021
·
IF 4.821
Wei Liu,Xiang Yang,Muyi Li,Qing-Wen Gui,Hongmei Jiang,Yuandong Li,Qinpeng Shen,Jianjun Xia,Xiaoying Liu
Due to the admirable conductivity and catalytic performance of d-Ti 3 C 2 T x and MWCNTs , a nanocomposite of d-Ti 3 C 2 T x /MWCNTs was synthesized with excellent electrochemical properties. After thoroughly characterized by SEM, TEM, XRD and XPS, the d-Ti 3 C 2 T x /MWCNTs composite was utilized as electrochemical sensing platform for detecting luteolin in peanut shell. The electrochemical behavior of luteolin at the modified electrode was systematically studied by CV and DPV. Under the optimum conditions, the modified electrode exhibited a commendable electrochemical response to luteolin in 0.1 M PBS (pH = 3.0). The linear response range for luteolin was 0.005–1.0 μM, with the detection limit (S/N = 3) of 1.6 nM. The results are better than many other literature results and the HPLC method. The developed sensing platform exhibited good anti-interference, stability and reproducibility. The practical feasibility of the method was also conducted by the determination of luteolin in peanut shell samples.
研究领域:
分析化学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Renjie Li,Zhuangwei Zhang,Jin Chen,Huihui Li,Hongjin Tang
Luteolin has extensive biological effects, but its low water-solubility and oral bioavailability have restricted its application. In this study, we successfully prepared new zein-gum arabic (GA)-tea polyphenols (TP) ternary complex nanoparticles (ZGTL) as a delivery system to encapsulate luteolin using an anti-solvent precipitation method. Consequently, ZGTL nanoparticles showed negatively charged smooth spherical structures with smaller particle size and higher encapsulation ability. X-ray diffraction revealed the amorphous state of luteolin in the nanoparticles. Hydrophobic, electrostatic, and hydrogen bonding interactions contributed to the formation and stability of ZGTL nanoparticles, as indicated by fluorescence and Fourier transform infrared spectra analyses. The inclusion of TP improved the physicochemical stability and luteolin retention rate of ZGTL nanoparticles by forming more compact nanostructures under different environmental conditions, including pH, salt ion concentration, temperature, and storage. Additionally, ZGTL nanoparticles exhibited stronger antioxidant activity and better sustainable release capacity under simulated gastrointestinal conditions due to TP incorporation. These findings demonstrate that ZGT complex nanoparticles have potential applications as an effective delivery system for encapsulating bioactive substances in food and medicine fields.
研究领域:
食品科学
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纳米技术
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药学
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生物技术
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产品关联
DOI
PubMed
高影响因子
ACS Omega
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2022
·
IF 4.132
Meng-Ting Song,Wen-Zhu Wang,Yao Lu,Rui-Min Han,Leif H. Skibsted,Jian-Ping Zhang
The interactions of luteolin (Lut) with bovine serum albumin (BSA) mediated by Cu(II) were investigated by spectroscopic, calorimetric, and molecular dynamic (MD) methods. Fluorescence studies showed that the binding of Lut to BSA was significantly enhanced by Cu(II) coordination with the number of binding sites and binding constant increasing from n = 1 and Ka = 3.2 × 105 L·mol–1 for Lut to n = 2 and Ka = 7.1 × 105 L·mol–1 for a 1:1 Cu(II)–luteolin complex, in agreement with the results from isothermal titration calorimetry (ITC). Site-specific experiments with warfarin and ibuprofen and MD confirmed that two binding sites of BSA were sequentially occupied by two Cu(II)–luteolin complexes. Cu(II) coordination increased the antioxidant activity of luteolin by 60% in the inhibition of carbonyl formation from the oxidation of amino groups in the side chain of BSA induced by the peroxyl radical ROO•; however, it counteracted the antioxidant effects of luteolin and played pro-oxidative roles in BSA aggregation induced by •OH.
研究领域:
生物化学
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分子生物学
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光谱学
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量热法
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分子动力学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2021
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IF 4.821
Xiaojing Si,Luchen Deng,Yingzi Wang,Mei Han,Yaping Ding
A novel poly alizarin red (AR) film incorporating carboxylic acid group functionalized multiwall carbon nanotube (f-MWCNT) modified glassy carbon electrode (GCE) was first prepared for the determination of luteolin (Lut). Scanning and transmission electron microscopy were applied for characterization of the surface morphology of the modified electrodes and cyclic voltammetry was used to investigate the electrochemical properties of the proposed electrode towards the oxidation of Lut. Optimization of the experimental parameters was performed with regard to pH, drop-coating volume of f-MWCNT, electro-polymerization cycles of alizarin red, and different potentials of i-t. And the mechanism for Lut detection based on AR/f-MWCNT/GCE was investigated by electrochemical methods. The linearity between the oxidation peak current and the Lut concentration was obtained in the range of 0.5 to 45 μM with a detection limit of 0.17 μM (S/N = 3). In addition, the AR/f-MWCNT/GCE electrode displayed high selectivity, good stability and reproducibility, and also successfully determined Lut in drug samples.
研究领域:
分析化学
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电化学
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纳米技术
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药物分析
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产品关联
DOI
高影响因子
Materials Today Bio
·
2022
·
IF 10.761
Chen Tan,Heng Fan,Jiahui Ding,Chaoqun Han,Yang Guan,Feng Zhu,Hui Wu,Yujin Liu,Wei Zhang,Xiaohua Hou,Songwei Tan,Qing Tang
Oxidative stress, caused by excessive production of reactive oxygen species (ROS), plays a crucial role in the occurrence and development of ulcerative colitis (UC). We developed ROS-responsive nanoparticles (NPs) as an efficacious nanomedicine against UC with oral administration. The NPs were fabricated with a d -α-tocopherol polyethylene glycol succinate-b-poly(β-thioester) copolymer (TPGS-PBTE) for ROS cleavage via the colitis-targeted delivery of luteolin (LUT), a natural flavonoid with good anti-inflammation and radical-scavenging activity. Owing to the thioether bond in the polymer main chain, the TPGS-PBTE NPs exhibited an ROS-responsive size change and drug release, which benefited the ROS-scavenging and selective accumulation of LUT in the inflamed colon. In a dextran sulfate sodium-induced acute colitis murine model, [email protected] NPs alleviated body weight loss, colon length shortening, and damage to the colonic tissues due to the suppression of ROS and proinflammatory cytokines (e.g., IL-17A, IL-6, interferon-γ, tumor necrosis factor-α), as well as upregulation of glutathione and anti-inflammatory factors (e.g., IL-10, IL-4). More importantly, [email protected] NPs regulated the inflammatory microenvironment by modulating the T helper (Th)1/Th2 and Th17/regulatory T cell (Treg) balance (i.e., increased numbers of Tregs and Th2 cells and decreased numbers of Th1 and Th17 cells), thus resolving inflammation and accelerating the healing of the intestinal mucosa. Additionally, the [email protected] NPs formulation enabled the reduction of the effective dose of LUT and showed excellent biosafety in the mouse model, demonstrating its potential as a targeted UC therapeutic oral preparation.
研究领域:
药学
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纳米技术
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胃肠病学
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免疫学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
COLLOID AND POLYMER SCIENCE
·
2022
·Vol. 300
·期 7
·861-871
·
IF 2.434
Mehmood Sahid,Uddin Md Alim,Yu Haojie,Wang Li,Amin Bilal Ul,Haq Fazal,Fahad Shah,Haroon Muhammad
In this study, cross-linked poly(cyclotriphosphazene- co -luteolin) (PCTPLT) nanospheres were prepared via polymerization-induced self-assembly. The particular band at 945 cm −1 in the Fourier transform infrared (FT-IR) spectra of the nanospheres demonstrated that the polymerization was successful. The morphology of the PCTPLT nanospheres was studied by scanning electron microscope (SEM) and the results revealed that the formation, stable growth, and particle size of the nanospheres strongly depended on the HCCP:LUT mole ratio. The X-ray diffraction (XRD) results showed that the crystalline peaks were completely lost after polymerization. The thermal properties of PCTPLT nanospheres were checked by thermogravimetric analysis (TGA) and the results exhibited that the increase in thermal properties was due to the strong covalently bonded cross-linked structure of the nanospheres. The doxorubicin (DOX) was loaded on the PCTPLT-3 nanospheres and their in vitro release behavior was checked at pH 4.0 and 7.4, and the results showed a cumulative release of 69.7% at pH 4.0 and 48.1% at pH 7.4 after 360 h. Graphical Abstract
研究领域:
材料科学
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药学
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产品关联
DOI
高影响因子
Frontiers in Nutrition
·
2022
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IF 6.59
Guo Wei,Luo Lianxiang,Meng Yan,Chen Wen,Yu Lixiu,Zhang Cong,Qiu Zhenpeng,Cao Peng
Background and purpose: Previous studies have indicated the protective effects of luteolin against non-alcoholic steatohepatitis (NASH), but the definite underlying mechanism still remains unclear. This study aimed to explore the metabolomic and metagenomic signatures of NASH with luteolin supplementation.Experimental approach: Mice were fed with a methionine–choline-deficient (MCD) diet containing 0.05% luteolin for 6 weeks. NASH severity was determined based on the liver histological observations, serum and hepatic biochemical measurements. Targeted metabolomics was conducted to identify differential metabolites in mice serum. 16S rRNA sequencing was conducted to assess the gut microbiota composition and function in mice colon.Results: In detail, luteolin treatment significantly alleviated MCD diet-induced hepatic lipid deposition, liver function damage, and oxidative stress. Targeted plasma metabolomics revealed that 5-hydroxyindole, LPE (0:0/22:5), indole 3-phosphate, and N-phenylacetylphenylalanine were remarkably elevated, and homogentisic acid, thiamine, KN-93, PC (16:1e/8, 9-EpETE), carnitine C9:1-OH, FFA (18:4) and carnitine C8:1 were significantly decreased in NASH group as compared to normal group, which could be profoundly reversed after luteolin treatment. 16S rRNA sequencing indicated that luteolin supplementation significantly increased Erysipelatoclostridium and Pseudomonas as well as decreased Faecalibaculum at genus level. Most importantly, a negative association between thiamine and Faecalibaculum was observed based on Spearman's correlation analysis, which may play an important role in the preventive effects of luteolin against NASH.Conclusion: Collectively, luteolin may alleviate the NASH by modulating serum metabolome and gut microbiome, which supports its use as a dietary supplement for NASH prevention.
研究领域:
药理学
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代谢组学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
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