BMEMat
·
2026
·
IF 17.2
Pai Peng,Dongchao Qiu,Yongyuan Kang,Qiaoxuan Wang,Xuelong Wang,Boyana Parvanova,Miroslav Karabaliev,Changyou Gao
Bioprosthetic heart valves (BHV) remain limited by progressive calcification, infection, and structural degeneration, leading to premature failure despite decades of clinical use. Herein, a rapid and efficient surface grafting strategy based on hyperbranched poly-L-lysine (HBPL) was developed to re-engineer the pericardial interface toward improved functional performance and translational potential. The HBPL-surface engineering enhanced the structural stability, resistance to enzymatic degradation, and anti-calcification performance of bovine pericardium (BP). Mechanistic investigations revealed that the hyperbranched topology and dense amino functionality of HBPL regulate calcium binding behavior and inhibit the nucleation and phase transformation of amorphous calcium phosphate. In addition, HBPL modification significantly reduced bacterial adhesion while maintaining favorable cytocompatibility and hemocompatibility. Arteriovenous shunt (AV-shunt) assays ex vivo demonstrated improved blood compatibility under flow conditions, and subcutaneous implantation in rats showed attenuated inflammatory responses and reduced calcification. Furthermore, balloon-expandable valves fabricated from HB-BP exhibited satisfactory hydrodynamic performance and durability exceeding 200 million fatigue cycles. Collectively, these results identify the HBPL-based surface engineering as a simple and versatile platform for developing BHVs with enhanced resistance to calcification and infection, improved durability, and promising translational potential.
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Ang Xu,Jie Gao,Huan Li,Xiyu Song,Bo Xu,Ying Liu,Yuanyuan Yu,Ping Wang,Qiang Wang,Man Zhou
Achieving a high working voltage window beyond the water electrolysis limit (1.23 V) is always a challenge for aqueous supercapacitors (SCs). Increasing the oxygen evolution reaction (OER) overpotential or hydrogen evolution reaction (HER) overpotential of the carbon-based electrode is an effective way to broaden the voltage window of SCs. In this work, we propose an efficient strategy of surface engineering to autonomously regulate the pH of the electrode/electrolyte interface microenvironment by using the amphoteric dissociation of amino acids, which suppress the OER and consequently broadens voltage window. L-Lysine was modified on the surface of carbon cloth to prepare the SC positive electrode. The voltage window of SCs based on L-Lysine modified electrode is enhanced from 1.2 V to 1.5 V and the energy density has increased by 59.42 % at 0.5 A g −1 . This is a promising strategy to design and engineer carbon-based aqueous SCs with a boosted energy density.
研究领域:
材料科学
·
电化学
·
能源存储
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2016
·Vol. 6
·期 10
·178
·
IF 2.69
Yuehua Zhang,Wu Lei,Yujuan Xu,Xifeng Xia,Qingli Hao
A novel, simple and selective electrochemical method was investigated for the simultaneous detection of dopamine (DA) and uric acid (UA) on a poly(l-lysine)/graphene oxide (GO) modified glassy carbon electrode (PLL/GO/GCE) by differential pulse voltammetry (DPV). The electrochemically prepared PLL/GO sensory platform toward the oxidation of UA and DA exhibited several advantages, including high effective surface area, more active sites and enhanced electrochemical activity. Compared to the PLL-modified GCE (PLL/GCE), GO-modified GCE and bare GCE, the PLL/GO/GCE exhibited an increase in the anodic potential difference and a remarkable enhancement in the current responses for both UA and DA. For the simultaneous detection of DA and UA, the detection limits of 0.021 and 0.074 μM were obtained, while 0.031 and 0.018 μM were obtained as the detection limits for the selective detection of UA and DA, using DPV in the linear concentration ranges of 0.5 to 20.0 and 0.5 to 35 μM, respectively. In addition, the PLL/GO/GCE demonstrated good reproducibility, long-term stability, excellent selectivity and negligible interference of ascorbic acid (AA). The proposed modified electrode was successfully implemented in the simultaneous detection of DA and UA in human blood serum, urine and dopamine hydrochloride injection with satisfactory results.
研究领域:
分析化学
·
生物医学工程
·
电化学传感器
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Fangcui Li,Ziyi He,Sihan Zhou,Ran Bi,Xianghua Yan,Chizhu Ding
The accurate quantification of lysine is crucial for animal health and nutrition. In this work, we report the development of a novel electrochemical sensor for rapid, highly selective, and pretreatment-free quantification of lysine in whole blood. The sensor was fabricated by electrodepositing Prussian blue on screen-printed carbon electrodes, followed by electropolymerization of a lysine-imprinted polymer layer. We implemented a computational design strategy, integrating molecular docking and density functional theory calculations, to screen and identify the optimal functional monomer with high lysine binding affinity. The developed sensor exhibited a linear response to lysine concentration in simulated serum, with a limit of detection of 28.1 μM. It demonstrated high selectivity against structural analogs and common blood interferents. Validation using swine serum samples showed excellent agreement with results obtained from an amino acid analyzer. Furthermore, the sensor successfully quantified lysine in whole blood samples without any pretreatment, providing a simple, reliable, and efficient platform for on-site lysine detection in resource-limited settings.
研究领域:
生物医学工程
·
分析化学
·
分子诊断
·
电化学传感
·
营养生理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Qiao Li,Shou-Jun Lai,Tingting Deng,Siyu Zhang,Teng Niu,Hai-xiong Shi,Hongmei Mu
A novel Schiff-base fluorescent probe (SA) has here been designed for lysine detection. A condensation reaction between thiophene-2-carboxaldehyde and 1,4-succinohydrazide was then used. The SA showed a selective “turn-on” fluorescence response (425 nm) to lysine in a pH-stable dimethyl sulfoxide (DMSO)/H₂O system (3:2, pH = 7.2), thereby achieving a detection limit of 8.21 × 10 −8 M. Both experimental and computational studies revealed that the SA formed a flexible V-shaped cavity via intramolecular non-covalent interactions. An adaptive encapsulation of lysine through synergistic O-H···N/O-H···O hydrogen bonds and σ-π interactions could, thereby, form a stable 1:1 complex. The present study has developed a rational design strategy for dynamic molecular probes and advanced high-performance sensing platforms for biomolecules.
研究领域:
分析化学
·
生物传感
·
有机化学
·
生物医学工程
✓ 已验证
DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 35
·25359-25368
·
IF 3.9
Yajun Jiang,Aolin Wu,Lin Yang,Jun Wu,Yue Liang,Zhigang Hu,Ying Wang
This study explored the preparation of a novel composite hydrogel based on deep eutectic solvent (DES) with lysine (Lys) and its application in pressure sensing and Fe3+ adsorption. DES was synthesized from acrylamide (AM) and urea (U) as hydrogen bond donors (HBD) with choline chloride (ChCl) as hydrogen bond acceptor (HBA), and Lys was used as a functional filler, and Lys/P(AM-U-ChCl) composite hydrogels were successfully prepared by frontal polymerization (FP) method. The structure of the hydrogels was characterized in depth using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The effects of Lys content on the mechanical properties, pH-responsive behavior, pressure-sensitive properties, and Fe3+ adsorption capacity of the hydrogels were further analyzed. It was found that the introduction of Lys significantly improved the compressive and pressure-sensitive properties of the hydrogels. The composite hydrogels exhibited excellent swelling equilibrium rates at different pH values. The capacitance change of the hydrogel with 0.5 wt% Lys at 200 g pressure was 2.12-fold higher than that of the hydrogel without Lys addition, and the adsorption efficiency of the hydrogel for Fe3+ was greatly enhanced. This study provides a new idea for the functionalized design of composite hydrogels and demonstrates their great application prospects in high-performance pressure sensors and heavy metal ion adsorption.
研究领域:
材料科学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Today
·
2024
·
IF 13.2
Xingyou Wang,Xiaoqing Sun,Jiahui Lei,Linsen Li,Nanhang Zhu,Yue Yu,Yating Zeng,Ke Kang,QiangYing Yi,Yao Wu
Bacterial infected wounds pose a significant threat to patient survival, necessitating prompt and effective therapeutic intervention. Biomaterials that are responsive to wound microenvironments, possess well-defined structures and are facile to fabricate are favored for fulfilling their mission of efficient wound repair. Drawing inspiration from the immunoregulatory properties of natural zwitterionic capsular polysaccharides (ZPS) and glucomannan (GM) as well as the biogenesis of nitric oxide (NO), this study introduces the zwitterionic lysine glycopolymers (ZLGs), which harmoniously integrate the antibacterial, anti-fouling and immunoregulatory functions into one entity, to effectively promote the healing of infected wounds without addition of any external cytokines or molecular drugs. The ZLGs, exhibiting excellent cytocompatibility, restore sustained NO biogenesis (∼7.1 μM) in wound environment and thus to clean bacteria fast and effectively (12 h clearance rate for S. aureus : 97 %; E. coli : 92 %). Notably, the cell-membrane affinitive ZLGs induce efficient aggregation of mannose receptors and polarize the macrophages to M2 phenotype via the PI3K-Akt/STAT3 pathway, resulting in a synergistic enhanced polarization efficiency increasement of 105 %, as compare to the cumulation of individual building modules. The bioinspired ZLGs accelerate the repair of S. aureus infected wounds in vivo, highlighting the significance of this multi-in-one construction strategy for chronic wound treatment.
研究领域:
生物材料学
·
医学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Dandan Guo,Diya Zheng,Ding Zhou,Changlun Tong,Shaohua Huang
Lysine (Lys) is an essential nutrient that plays a crucial role in the growth and development of living organisms. Chiral analysis of Lys holds significant importance for ensuring the safety of food, pharmaceuticals, and health products. In this work, an achiral Zr-based metal organic frameworks (MOFs), UiO-66-NH 2 , was proposed as a fluorescent probe to achieve rapid response to l -lysine (L-Lys) in solution. Additionally, Zr 4+ in the skeleton of UiO-66-NH 2 framework exhibited different binding capacities towards Lys enantiomers, leading to distinct fluorescence responses for L-Lys and d -lysine (D-Lys). Leveraging these properties, the UiO-66-NH 2 probe enabled accurate determination of L-Lys concentrations in solution, as well as the enantiomeric excess (ee) values of Lys solutions. Notably, in the detection of Lys enantiomers, the achiral UiO-66-NH 2 acted as both a chiral selector and a fluorescent indicator, greatly improving the efficiency and stability of the detection system. The probable mechanism was further elucidated by pH titration experiments and density functional theory calculations. Additionally, the general applicability of this mechanism was validated by similar amino MOFs. The application of the UiO-66-NH 2 fluorescent probe in analysis of infant formula milk powders and liquid milk samples confirmed the reliability of the method. Moreover, the construction of fluorescence test paper by immobilizing UiO-66-NH 2 onto filler papers enabled the rapid identification of Lys enantiomers. Compared to previous fluorescent analyses of chiral amino acids assisted by additional metal ions, this study presented a novel approach and methodology that offers high efficiency, stability, reproducibility and reusability for the identification and detection of Lys enantiomers, highlighting the potential of the UiO-66-NH 2 fluorescent probe in advancing analytical techniques.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Chufan Yang,Yan Wang,Zhiyuan Zhang,Chen Wang,Fangzheng Yu,Zheng Zhao,Zhenyu Wang,Lingling Zhang
The healing of bacteria-infected wounds has long posed a significant clinical challenge. Traditional hydrogel wound dressings often lack self-healing properties and effective antibacterial characteristics, making wound healing difficult. In this study, a bioactive small molecule cross-linking agent 4-FPBA/Lys/4-FPBA (FLF) composed of 4-formylphenylboronic acid (4-FPBA) and lysine (Lys) was utilized to cross-link guar gum (GG) and a tannic acid/iron (TA/Fe 3+ ) chelate through multiple dynamic bonds, leading to the formation of a novel self-healing hydrogel dressing GG-FLF/TA/Fe. The hydrogel exhibited excellent stretchability and self-healing ability, which enabled it to adapt to irregular wound sites. Meanwhile, the hydrogel demonstrated remarkable antibacterial efficacy (>99 %) facilitated by the synergistic effects of photothermal properties and aromatic Schiff bases. Additionally, it had adjustable rheological properties, good mechanical characteristics, conductivity, antioxidant characteristics and biocompatibility. Notably, the GG-FLF/TA/Fe hydrogel dressing irradiated with NIR displayed superior therapeutic effects in a mouse wound infection model (wound healing rate: 94.8 %), promoting recovery from bacterially infected wounds by enhancing collagen deposition, facilitating the formation of skin appendages and blood vessels, and regulating inflammatory factors. In summary, this study presented a novel approach to prepare biologically active antibacterial polysaccharide hydrogels and highlighted the substantial potential of this hydrogel as a biomedical antibacterial dressing.
研究领域:
生物医学工程
·
材料科学
·
感染与免疫
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Feng Qi,Yangyang Li,Tanglei Zhang,Weiyu Dou,Riyue Shu,Zhikan Yao,Jing Wang,Lin Zhang
Segregating airborne particulate matters (PMs, especially PM 1.0 ) with high transmissibility of bacterial and viral pathogens is crucial for ensuring outdoor breathing safety. Electrospun nanofiber membranes (ENMs) have shown great promise for this purpose. However, the application of traditional ENMs is usually plagued by bacterial growth causing severe public health concern. Although some research has been done to overcome this challenge by doping antibacterial materials, the potential negative effects of these materials on the human body cannot be ignored. In this study, the ε-Poly-L-lysine (ε-PL), a green broad-spectrum antibacterial reagent, was grafted onto the surface of polydopamine (PDA) coated polyacrylonitrile (PAN) ENM via addition of Schiff base and Michael reaction. Experimental results indicated that the introduction of the ε-PL is key to the antibacterial performance of the membranes, and the antibacterial efficacy of E. coli and B. subtilis can reach to 99.86 % and 99.99 % respectively. Even after 7 days, the antibacterial efficiency of membrane remained at a high level. Additionally, the uniform structure of membranes endowed it with a high filtration efficiency of 80.9 % for PM 1.0 particles with a moderate transmembrane pressure drop (TMP) of 250 Pa and the corresponding quality factor is approximately 0.0066 Pa −1 . This study has far-reaching implications for the design of green antibacterial nanofibrous membranes to realize the segregation of harmful PMs.
研究领域:
材料科学
·
环境科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shuai Li,Jianqin Zhang,Hao Wang,Xingyun Zhao,Lin Yang,Lin Chai,Yangyang Liu,Zhangwu Zhao,Bernard Moussian,Abeer El Wakil,Kun Yan Zhu,Zhanghua Zeng,Jianzhen Zhang
RNA interference (RNAi)-based biopesticides, recognized by Science as one of the top ten scientific breakthroughs of 2024, are poised to drive the third revolution in pesticide development. However, the practical applications of RNAi face critical challenges, particularly in overcoming delivery barriers, such as double-stranded RNA (dsRNA) degradation and poor cellular uptake. In this study, we developed a biologically derived melanin nanoparticles (MNP) functionalized with poly- l -lysine (PLL) to deliver dsRNA in Locusta migratoria , a major agricultural pest. The functionalized melanin nanoparticles (MNP-PLL) self-assemble with dsRNA to form MNP-PLL/dsRNA through electrostatic interactions. We evaluated their stability under different environmental conditions, such as in midgut fluid, under various temperature and UV irradiation, demonstrating significantly enhanced resistance to degradation. Bioassays showed substantial improvements in RNAi efficiency and insect mortality. Using fluorescent labeling and pharmacological inhibitors, we further investigated the cellular uptake mechanisms. In contrast to naked dsRNA, chitosan/dsRNA, carbon quantum dots/dsRNA and Spc/dsRNA, MNP-PLL/dsRNA activated three distinct endocytic pathways: clathrin-mediated endocytosis, caveolin-mediated endocytosis, and macropinocytosis, facilitating efficient cellular internalization. Acute toxicity assays confirmed excellent biocompatibility and safety of MNP-PLL to the Western honey bee and zebrafish. This study represents the first application of PLL-functionalized melanin as an efficient nanocarrier to generate MNP-PLL/dsRNA for agricultural pest control, highlighting their strong potential as a next-generation dsRNA delivery platform for sustainable pest management.
研究领域:
农业生物技术
·
纳米生物技术
·
分子昆虫学
·
RNA干扰
·
害虫管理
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Ning Cui,Junmin Qian,Weijun Xu,Minghui Xu,Na Zhao,Ting Liu,Hongjie Wang
In the present study, poly( N ɛ -acryloyl- l -lysine)/hyaluronic acid (pLysAAm/HA) interpenetrating network (IPN) hydrogels were successfully fabricated through the combination of hydrazone bond crosslinking and photo-crosslinking reactions. The HA hydrogel network was first synthesized from 3,3′-dithiodipropionate hydrazide-modified HA and polyethylene glycol dilevulinate by hydrazone bond crosslinking. The pLysAAm hydrogel network was prepared from N ɛ -acryloyl- l -lysine and N , N ′-bis(acryloyl)-( l )-cystine by photo-crosslinking. The resultant pLysAAm/HA hydrogels had a good shape recovery property after loading and unloading for 1.5 cycles (up to 90%) and displayed a highly porous microstructure. Their compressive moduli were at least 5 times higher than that of HA hydrogels. The pLysAAm/HA hydrogels had an equilibrium swelling ratio of up to 37.9 and displayed a glutathione-responsive degradation behavior. The results from in vitro biocompatibility evaluation with pre-osteoblasts MC3T3-E1 cells revealed that the pLysAAm/HA hydrogels could support cell viability and proliferation. Hematoxylin and eosin staining indicated that the pLysAAm/HA hydrogels allowed cell and tissue infiltration, confirming their good in vivo biocompatibility. Therefore, the novel pLysAAm/HA IPN hydrogels have great potential for bone tissue engineering applications.
研究领域:
材料科学
·
生物医学工程
·
生物材料
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）