JOURNAL OF ETHNOPHARMACOLOGY
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2018
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IF 3.115
Wenhao Xu,Meiqun Hu,Qihong Zhang,Jingbo Yu,Weike Su
Ethnopharmacological relevance Cassia occidentalis Linn. is a traditional ayruvedic edible shrub containing anthraquinones (AQs) as the principle active constituents. In folk medicine, it has a variety of uses including treatment of whooping cough (‘pertussis’) and inflammatory diseases . Despite these applications, limited data are available to validate the effects of C. occidentalis AQs on airways inflammation in asthma. Aim of the study To explore the anti-inflammatory potential of AQs extracted from C. occidentalis using an in vivo model of ovalbumin (OVA)-induced asthma. Materials and methods Extraction and optimization of AQs from C. occidentalis was performed by mechanochemistry. Allergic asthma in BALB/c mice was sensitized and challenged by OVA, and the effects of AQs investigated in a mouse model. OVA-specific IgE concentrations in serum, and Th1/Th2 cytokine (IL-4, IL-5, IL-13 and IFN- γ ) concentrations, inflammatory cell counts and classification in bronchoalveolar lavage fluid (BALF) were determined. Histopathological evaluation of lung tissue was performed using hematoxylin and eosin (H&E), and periodic acid-schiff (PAS) staining. Th1/Th2 cytokine mRNA expression was analyzed using the 2 -ΔΔCt method. Results Treatment with AQs decreased inflammatory cell counts and production of Th2 cytokines (IL-4, IL-5 and IL-13) in BALF, and OVA-specific IgE in serum. In contrast,Th1 cytokine IFN- γ production in BALF was promoted. AQs also decreased mRNA expression of Th1/Th2 cytokine in lung tissue. Histological studies demonstrated that AQs substantially inhibited OVA-induced cellular infiltration, mucus hypersecretion and goblet cell hyperplasia in the lung. Conclusions These findings demonstrated the inhibitory effects of AQs, derived from C. occidentalis, on OVA-induced allergic asthma in mice. The results suggest a promising ethnopharmacological use for AQs in patients with asthma.
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FOOD HYDROCOLLOIDS
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2022
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IF 11.504
Ruiling Li,Hui Xue,Binghong Gao,Huilan Liu,Tianfeng Han,Xiaobo Hu,Yonggang Tu,Yan Zhao
To investigate the effect of oil droplets surface film type and droplets size on the physicochemical properties and digestibility of thermally induced ovalbumin–oil emulsion gels, Tween 80 and ovalbumin were selected as individual emulsifiers for the emulsification of sunflower oil at different homogenization speeds (6000, 8000, and 10000 rpm), and the formed emulsions were mixed with ovalbumin matrix solution and then heated to form heat-induced ovalbumin emulsion gels (OEG). The OEG with ovalbumin as emulsifier had higher hardness, water holding capacity (WHC), and surface hydrophobicity, and lower digestibility, spin−spin relaxation time ( T 2 ) and free sulfhydryl content than those of the OEG with Tween 80 as the emulsifier ( p < 0.05). As the oil droplets size decreased, the hardness increased, WHC increased, the amide A band shifted to lower wavenumbers, digestibility decreased, T 2 decreased, free sulfhydryl content decreased, and surface hydrophobicity increased for both OEG ( p < 0.05). These results collectively indicated that the physicochemical properties of the OEG are influenced by the property of the oil interfacial film and the size of the oil droplets. The oil droplets surrounded by the interfacial protein film improved the performance of the OEG greatly, and the proteins within its OEG formed a dense gel network structure through hydrophobic aggregation, disulfide bond crosslinking, and hydrogen bonding. The oil droplets with smaller sizes favored the enhancement in the physicochemical properties of the OEG. Changes in the size of oil droplets and the type of film wrapped around them regulated the digestibility of the OEG.
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FOOD CHEMISTRY
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2022
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IF 9.231
Congnan Cen,Jian Chen,Weiqiang Wang,Jie Zhang,Xiangying Yang,Linglin Fu,Yanbo Wang
This study aims to reveal the mechanism of the interaction between folic acid (FA) and egg ovalbumin (OVA) through the method of multi-spectroscopic, molecular docking, and molecular dynamics simulation in order to probe OVA as the possibility of a carrier of unstable vitamins. The results of the fluorescence spectra indicated a static quenching in the OVA-FA with a strong affinity of 6.998 × 10 4 M −1 . At the same time, the complex formed by FA and OVA has changed the microenvironment. The measurement results of circular dichroism and particle size showed that FA and OVA gradually formed larger particles without changed the secondary structure of the protein. In addition, the results of molecular simulations indicated that the interaction between OVA and FA is mainly stabilized by strong hydrophobic and hydrogen bonds. This research was expanded the application prospect of dietary protein OVA as a transportation and protection system of vitamin substances.
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PULMONARY PHARMACOLOGY & THERAPEUTICS
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2021
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IF 3.41
Bo Zou,Yue Fu,Chaofan Cao,Dianzhu Pan,Wei Wang,Lingfei Kong
Allergic asthma is a common airway inflammatory disorder with increasing morbidity and mortality worldwide. Gentiopicroside (GPS) is a secoiridoid glycoside compound that exhibits anti-inflammatory property. However, the effect of GPS on allergic asthma has not been reported yet. In this study, we investigated the role of GPS in a mouse model of ovalbumin (OVA)-induced allergic asthma and explored its potential mechanism. Mice were sensitized with OVA and gavaged with 20, 40, or 80 mg/kg GPS. Administration of GPS decreased lung wet-to-dry weight ratio. Histological analysis of H&E and PAS staining showed that GPS treatment alleviated inflammatory cell infiltration and goblet cell hyperplasia in lung tissue of OVA-sensitized mice. Moreover, GPS inhibited the recruitment of inflammatory cells including total cells, macrophages, eosinophils , lymphocytes and neutrophils and the secretion of T helper type 2 (Th2) cytokines (interleukin (IL)-4, IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF) of OVA-sensitized mice in a dose dependent manner. The levels of OVA-specific immunoglobulin E (IgE) and pro-inflammatory tumor necrosis factor (TNF)-α were also attenuated by GPS treatment. Interestingly, GPS upregulated the expression of silent information regulator 1 (SIRT1) while downregulated the expression of acetyl-nuclear factor kappa B (NF-κB) p65 in lung tissue of OVA-sensitized mice. Furthermore, treatment with an SIRT1 inhibitor (EX-527) partially abolished the inhibitory effect of GPS on OVA-induced airway inflammation, suggesting that the anti-inflammation of GPS might be achieved through regulating SIRT1/NF-κB p65 signaling pathway . These findings indicate that GPS might be a novel drug candidate in the treatment of allergic asthma.
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FOOD HYDROCOLLOIDS
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2020
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IF 7.053
Shugang Li,Kangping Wang,Qun Huang,Fang Geng
The present study constructed a new gellable emulsion with the aid of microwave pretreatment to encapsulate pomegranate seed oil (PSO). The results showed that ovalbumin (OVA), inulin (IN) and carrageenan (κ-car) were adsorbed on the PSO drop surface to form an OVA–IN–PSO emulsion, and then, gelation of the emulsion occurred by heating with the assistance of microwave pretreatment. The properties of the OVA–IN–PSO emulsion gel, including the water holding capacity , fixed water ratio, texture properties, and rheological properties, were improved by microwave pretreatment. Microscopic observation confirmed that the OVA–IN–PSO gel was an oil-in-water type with a dense structure. Moreover, the denaturation temperature of the OVA–IN–PSO emulsion increased from 70.7 °C (0 min) to 76.3 °C (10 min). Benefitting from the protective effect of the OVA–IN–PSO gel, the stability of pomegranate seed oil was significantly improved. Lipid oxidation (peroxide value) and the loss of polyunsaturated fatty acids during pomegranate seed oil storage were suppressed. These results suggested that microwave pretreatment can improve the properties of OVA–IN–PSO gel as well as the oxidation stability of pomegranate seed oil.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2019
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IF 3.714
Yang Chen,Zhenshun Li,Xiangzhou Yi,Hairui Kuang,Baomiao Ding,Weiqing Sun,Yangchao Luo
Ovalbumin (OVA) is widely used in the food industry due to its good emulsifying properties. However, polyphenols and polysaccharides in food may influence the interfacial properties of OVA. In our work, carboxymethylcellulose (CMC) and tannic acid (TA) were mixed with OVA at pH 4.0 and 7.0. The results indicated that there was noncovalent binding among OVA, CMC and TA, which affected the spatial structures of OVA. The emulsifying ability of OVA stabilized emulsions decreased after binding with CMC and/or TA, due to the reduction in interfacial activities. Additionally, TA reduced the emulsion stability. However, the stability of the emulsions with and without TA could be improved by CMC, especially at pH 4.0. CMC reduced the binding affinity of OVA and TA and increased the electrostatic repulsion of the continuous phase, resulting in increased stability against creaming. Our study was conducted to provide a reference for OVA use in the food industry.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Yating Xiong,Yang Chen,Xiangzhou Yi,Zhenshun Li,Yangchao Luo
The poor interfacial stability of protein-stabilized high internal phase Pickering emulsions (HIPEs) is a major hurdle to realize their practical applications in food processing. The emulsifying stability is not only related to the protein itself, but also dependent upon the oil phases. In this study, four plant-based oils were studied to understand their respective effects on the interfacial stability of HIPEs prepared by ovalbumin (OVA) and ovalbumin-tannic acid complex (OVA-TA). Our findings revealed that the interfacial activities were closely related to the physicochemical properties of the oil phase, such as the number of carbon‑carbon double bonds in the unsaturated fatty acids, melting point, and polarity. The emulsifying abilities were ranked as palm oil > soybean oil > olive oil > perilla oil. OVA-TA stabilized HIPEs exhibited excellent emulsifying stability compared with free OVA stabilized ones. This work provided a unique insight into understanding the interfacial stabilization mechanisms for protein-stabilized HIPEs with different kinds of oil phases.
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FOOD HYDROCOLLOIDS
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2023
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IF 10.7
Xin Hu,Wen-xuan Hu,Hong-yu Lu,Song Liu,Sheng-qi Rao,Zhen-quan Yang,Xin-an Jiao
This study investigated the effects of enzyme action time (1–5 h) on the physicochemical properties, structure, and functionality of transglutaminase-catalyzed ovalbumin (OVA) in the presence of oligochitosan (OCS). Physically combining OVA and OCS led to a substantial increase in particle size and polydispersity index, rendering the mixed system unstable. With the increase of enzyme action time, the particle size of glycosylated and cross-linked OVA (GC-OVA) decreased first and then increased, reaching a minimum value of 411 nm at 4 h. Moreover, the decrease of free amino groups content at 4 h reflected the occurrence degree of glycosylation and cross-linking reaction, which was close to saturation. Diverse methods were used to analyze variations in the molecular structure of OVA, confirming changes in the primary, secondary, and tertiary structures, and the microstructure. The glycosylation grafting and cross-linking with the appropriate extent of the enzymatic action time is the primary reason for improving the foaming , emulsification properties, and DPPH activity of OVA. Moreover, GC-OVA had greater apparent viscosity, G′ and G″ than the other control systems (the untreated OVA and the physical mixture of OVA and OCS) and significantly affected the hardness and cohesion properties in the gel's texture (5.6 and 2.4-fold that of the untreated OVA, respectively). This study provides information for further understanding the effect of glycosylation grafting caused by enzyme cross-linking time on OVA. The study also demonstrates the possibility of obtaining new protein compositions to expand the applications of food proteins.
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SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Xuan Fu,Long Sheng,Yisha Yu,Meihu Ma,Zhaoxia Cai,Xi Huang
Fluorescence resonance energy transfer (FRET), a near-field energy transfer from a fluorescent donor to a fluorescent acceptor within close proximity, has been widely used in biosensing. In this study, a rapid and universal sensor for the detection of ovalbumin (OVA) using nitrogen, oxygen, phosphoric co-doped CDs (NOP-CDs) and graphene oxide (GO) was established based on the FRET technology. First, novel NOP-CDs peak at 443 nm were synthesized by a one-pot hydrothermal method using egg powder and phosphorus acid. Then, in the absence of OVA, the fluorescence of OVA-antibody modified NOP-CDs was quenched by GO via electrostatic attraction and π-π stacking interaction due to the FRET effect. The introduced OVA competed to displace the GO by special binding with the anti-OVA, resulting in the recovery of the fluorescence. This sensor displayed a linear response to OVA from 0.5 to 15 μg/mL with a detection limit of 153 ng/mL and was successfully applied to the detection of the OVA sample with recoveries ranging from 99.25 to 118.90%. In summary, the green and easy-to-synthesize nano-material was used in this method, the accurate detection results, and the simple process exhibited the great potential of this method for practical applications.
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Journal of Functional Foods
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2024
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IF 3.8
Jiahao Liao,Jianyu Huang,Xiaoyi Chi,Xiaoqiong Li,Jian Kuang,Jianqiang Li,Xiangyu Bian,Jinzhu Pang,Jinjun Li,Weiwei Wang
In this study, D-mannose-modified nanoliposomes (MLipo@OVA) were developed to facilitate the targeted delivery of ovalbumin (OVA) to macrophages. The subsequent anti-inflammatory, antioxidant, and macrophage-polarizing effects of OVA were investigated. The nanoliposomes were designed with DSPE-PEG and DPPC to enhance the bioavailability and stability of OVA. In vitro analysis showed that MLipo@OVA significantly increased macrophage uptake, with D-mannose further enhancing this effect. MLipo@OVA reduced pro-inflammatory cytokines (TNF-α, IL-1β) and increased anti-inflammatory IL-10 levels in LPS-induced macrophages, primarily via the inhibition of the TLR4/NF-κB pathway. Furthermore, ROS levels were decreased, SOD and GSH activity was enhanced, the Nrf2/HO-1 pathway was activated, and the compound exhibited strong antioxidant activity. MLipo@OVA also promoted M2 macrophage polarization by upregulating CD206 and Arg-1 and downregulating iNOS. Overall, these results indicate that MLipo@OVA could be used as a nanomedicine to facilitate targeted anti-inflammatory and antioxidant therapies.
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JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
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2024
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IF 3.7
Pei Haibing,Lin Zhiyin,Yao Kexin,Luo Yeqing,Tong Ping,Chen Hongbing,Wu Yong,Wu Zhihua,Gao Jinyan
Ovalbumin (OVA) is a major allergen in eggs and could induce severe allergic reactions in sensitive individuals, where the innate immune system works as a regulator. The mechanism of how innate immunity adjusts to food allergy is relatively well-studied, however, the effects of allergen uptake on the innate immune system remain unclear. Therefore, the Caenorhabditis elegans ( C. elegans ) model was utilized to assess the effects of OVA on its innate immune system. OVA enhanced the immune response of C. elegans with higher survival rates under Pseudomonas aeruginosa infection. Moreover, sustaining OVA treatment improved the health states that were reflected in the prolonged lifespan, alleviated oxidative stress, accelerated growth, and promoted motility. RNA-sequencing analysis and the slow-killing assays in the mutants of insulin/IGF-1 signaling (IIS)-related genes confirmed that IIS was necessary for OVA to regulate innate immunity. Besides, OVA activated SKN-1 temporarily and facilitated the nuclear localization of DAF-16 for improving immunity and health status in C. elegans . Together, OVA could enhance the innate immune responses via DAF-16 and SKN-1 pathways in the IIS of C. elegans , and this work will provide novel insights into the regulation of innate immunity by OVA in higher organisms.
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Food Bioscience
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2026
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IF 6.2
Qi Wu,Xiang Ruan,Meng qi Li,Xu Yang,Jing Ren
Food allergy represents a group of disorders driven by abnormal immune responses, with a prevalence that has been rising steadily in recent years. Research indicates that human milk oligosaccharides and gut probiotics play an essential role in early-life immune regulation and the establishment of allergic tolerance. This study preliminarily explored the alleviating effects of a combined intervention with HMOs and Lc. paracasei 1 in OVA-induced food-allergic mice. The results demonstrated that the combined intervention markedly relieved allergic symptoms, reduced symptom scores, lowered serum IgE levels, rebalanced Th1/Th2 cytokines, and enhanced intestinal barrier integrity. 16S rRNA sequencing revealed that the HMOs and Lc. paracasei 1 combination significantly increased gut microbiota α-diversity and promoted the enrichment of beneficial bacteria, including Lactobacillus and Bifidobacterium . Concurrently, levels of key microbial metabolite, particularly short-chain fatty acids, rose substantially. Further untargeted Metabolomics analysis identified significant alterations in multiple differential metabolites across treatment groups, such as indole-3-acetic acid, indole-3-propionic acid, tryptophan, taurocholic acid, deoxycholic acid, and arachidonic acid. KEGG pathway enrichment analysis showed these metabolites were primarily involved in tryptophan metabolism, fatty acid metabolism, bile acid metabolism, and amino acid biosynthesis. Microbiome-metabolite correlation analysis further indicated that the abundances of Lactobacillus and Bifidobacterium were strongly positively correlated with indole metabolites and SCFAs. These findings suggest that the combined intervention of HMOs and Lc. paracasei 1 may alleviate food allergy symptoms by modulating gut microbiota composition and the metabolic profile, thereby influencing the body’s immune response.
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