RSC Advances
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2021
·Vol. 11
·期 8
·4444-4453
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IF 3.361
Ruotong Liu,Chanling Yuan,Yin Feng,Jiayi Qian,Xiaoting Huang,Qiutong Chen,Shuyuan Zhou,Yin Ding,Bingbing Zhai,Wenjie Mei,Liangzhong Yao
Ru(II) complexes have attracted increasing attention as promising antitumor agents for their relatively low toxicity, high affinity to DNA molecules, and correlation with multiple targets. Meanwhile, quinolones are synthetic antibacterial agents widely used in the clinical practice. In this paper, two novel Ru(II) complexes coordinated by levofloxacin (LOFLX), [Ru(bpy)2(LOFLX)]·2ClO4 (1), and [Ru(dmbpy)2(LOFLX)]·2ClO4 (2) (bpy = 2,2′-bipyridine, dmbpy = 4,4′-dimethyl-2,2′-bipyridine) were synthesized with high efficiency under microwave irradiation and characterized by ESI-MS, 1H NMR, and 13C NMR. The binding behavior of these complexes with double-strand calf thymus DNA(CT-DNA) was investigated using spectroscopy, molecular docking, and density functional theory calculations. Results showed that 2 exhibited higher binding affinity than 1 and LOFLX. Further studies showed that 2 could induce the G2/M phase arrest of A549 cells via DNA damage. In summary, these results indicated that 2 could be developed as a potential anticancer agent in treatment of lung cancer through the induction of cell cycle arrest at G2/M phase by triggering DNA damage.
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ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Yaqin He,Caizhi Lv,Xiandeng Hou,Lan Wu
Taking advantage of the superior biocompatibility, good stability in a wide pH and temperature range, as well as its strong affinity with DNA of hydroxyapatite (HAp), tetrahedral DNA nanostructures (TDNs) conjugated with AS1411 aptamer ( anti -nucleolin overexpressed on tumor cell membranes) were employed as affinity ligands to construct a novel mono-dispersed HAp based probe with Gd 3+ doping (Apt-TDNs-GdHAp) for MR imaging. The adsorption of TDNs on the nano-HAp surface facilely accomplished the construction of the Apt-TDNs-GdHAp probes. Meanwhile, the use of hydrophilic TDNs not only favored the phase-transfer from the oil phase to the aqueous phase, but also enhanced the mono-dispersion of this probe due to the well-ordered distribution of TDNs on the surface of nano-HAp. Moreover, Apt-TDNs-GdHAp probe with a better mono-dispersion and crystalinity achieved twice higher longitudinal relaxivity (r 1 value) than that of GdHAp synthesized by microwave-assisted method (Microwave-GdHAp), exhibiting much more excellent T 1 -weighted imaging performance. With the introduction of TDNs, the stability and the tumor-targeting accessibility were also greatly improved, showing its great potential for further bio-applications.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2020
·
IF 4.872
Ling Ou,Haixia Wang,Zhidi Wu,Panpan Wang,Li Yang,Xiaoyun Li,Kehuan Sun,Xiaofeng Zhu,Ronghua Zhang
Cadmium is an environmental metal pollutant that has been a focus of research in recent years, which is reported to cause bone disease; however, its skeletal toxicity and the mechanism involved are not yet fully known. Therefore, this study used MC3T3-E1 subclone 14 cells to determine the mechanism of cadmium toxicity on bone. Cadmium chloride (Cd) significantly reduced cell viability in a concentration-dependent manner. Exposure to Cd inhibited osteoblast-related proteins (Runx2, Col-1, STC2) and decreased alkaline phosphatase (ALP) activity. Cd caused Exportin-1 accumulation and induced DNA damage. Cd significantly down-regulated caspase 9 and induced cleaved-PARP, cleaved-caspase 3 protein level. Treatment with JNK inhibitor, SP600125, suppressed cadmium-induced elevation in the ratio of phosphorylation of JNK to JNK. Inhibition of caspase with pan-caspase inhibitor, Z-VAD-FMK, prevented MC3T3-E1 subclone 14 cells from cadmium-induced reduction of Runx2, STC2, caspase 9, and accumulation of cleaved PARP and cleaved caspase 3. Cd-induced cell survival enhanced by SP600125 but rescued by Z-VAD-FMK or KPT-335. These results suggest that cadmium cytotoxicity on bone involved exportin 1 accumulation, phosphorylation of JNK, induction of DNA damage and pro-apoptosis, which was induced by activation of caspase-dependent pathways.
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Scientific Reports
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2020
·Vol. 10
·期 1
·1-8
·
IF 3.998
Tan Xiao-Hui,Li Yu-Bin,Liao Yan,Liu Hua-Zhong
In this work, an enzyme-free fluorescence resonance energy transfer (FRET) strategy was established for rapid and specific detection of the DNA sequence from Vibrio parahaemolyticus (VP) using hybridization chain reaction (HCR) amplification and triplex DNA. The triplex forming oligonucleotide (TFO) was labelled with carboxyfluorescein (FAM) as fluorescence donor, and hairpin sequence H1 was labelled by tetramethylrhodamine (TAMRA) as fluorescence receptor. In the present target VP DNA, the hairpin structure of molecular beacon (MB) was opened, the free end was released and hybridized with H1-TAMRA, and the HCR reaction was triggered by the alternate supplementation of H1-TAMRA and H2 to produce the notch double helix analogue. After the addition of TFO-FAM, a triplex structure was formed between HCR products (H1-TAMRA/H2) and TFO-FAM. A close contact between the donor and the receptor resulted in FRET. Under the optimal conditions, the fluorescence quenching value was inversely proportional to the concentration of target VP DNA in the range of 0.1–50 nmol L −1 , and the detection limit was 35 pmol L −1 .
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BIOSENSORS & BIOELECTRONICS
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2020
·
IF 10.257
Bing Han,Lingjun Sha,Xiaomeng Yu,Mei Yang,Ya Cao,Jing Zhao
Breast cancer is the most common malignant disease among women worldwide. Nowadays, combined therapy against several therapeutic targets is becoming a promising treatment to enhance the survival rate of the patients with some lethal subtypes, and also proposes high demand on the discrimination of the co-existing targets in breast cancer. In this work, we designed in situ automatous DNA assembly reaction and applied it for the simultaneous identification of dual therapeutic targets using electrochemical techniques . Taking triple-negative breast cancer cell MDA-MB-231 as a model, chained strand displacement reactions were initiated after the capture probes recognized the surface biomarkers, epidermal growth factor receptor and intercellular adhesion molecule-1, respectively. Then, an increased electrochemical signaling was created to reveal the co-expression of the two targets using quantum dots as electrochemical labeling. Electrochemical results demonstrated high sensitivity and specificity of our method toward the identification of the coexisted therapeutic targets even in the serum samples, which also allowed to monitor the enhanced efficiency of combined therapy. Therefore, our method suggested a potential use in the accurate identification of therapeutic targets in breast cancer that might provide more information to facilitate the combined therapy in clinic.
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MICROCHIMICA ACTA
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2020
·Vol. 187
·期 6
·1-9
·
IF 6.232
Abedalwafa Mohammed Awad,Tang Zhimin,Qiao Yansha,Mei Qianqian,Yang Gang,Li Yan,Wang Lu
The preparation of portable colorimetric biosensor strips is described by combining aptamer-immobilized electrospun nanofiber membranes (A-NFMs) with signal probes (DNA-conjugated gold nanoparticles (AuNPs)) for determination of kanamycin (KMC) as a model analyte. The A-NFMs were decorated with complementary single-stranded DNA (cDNA) of KMC aptamer-conjugated AuNPs (cDNA@Au) to get the colorimetric biosensor strips. The constructed biosensor strips showed a significant absorbance decreasing band at 510 nm which induce a visual color change from pink to white when exposed to KMC, with a low detection limit of 2.5 nM (at S/ N = 3). The effect is due to disassembling of cDNA@Au from NFMs in the presence of KMC because the aptamer has a higher affinity to KMC than its complementary DNA, which resulted in replacing cDNA@Au with KMC. Satisfactory performance was observed in real sample (drinking water and milk) analysis with a recovery of 98.9–102.2%. The constructed colorimetric biosensor test strips hold great application promise for food safety control.
Schematic representation of biosensor strips for kanamycin detection prepared with the cDNA@Au immobilized aptamer-based cellulose acetate nanofibers.
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ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
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2020
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IF 3.292
Zuntao Wu,Lin Bai,Runqi Tu,Lijie Zhang,Yue Ba,Huizhen Zhang,Xing Li,Xuemin Cheng,Wenjie Li,Hui Huang
Lead (Pb) is recognized as a potent inducer of synaptic toxicity generally associated with reduced synaptic transmission and increased neuronal fiber excitability, becoming an environmental risk for neurodegenerative processes. Despite numerous toxicological studies on Pb have been directed to the developing brain, attention concerning long-term consequences of pubertal chronic Pb exposure on neuronal activity is still lacking. Thus, we exposed 4-week-old male mice to 0.2 % lead acetate solution for one month, then, conducted behavioral tests or extracted brain homogenate from mice prefrontal cortex (PFC) and hippocampus at the age of 4, 13 and 16-month-old respectively. Our results showed that treated mice exhibited an evident increase in latency to reach platform following pubertal Pb exposure and aging. The increase of 8-OHdG revealed evident neural DNA oxidative damage across time upon pubertal Pb exposure. In the hippocampus of lead exposed mice at three age nodes, the expression of brain-derived neurotrophic factor precursor (proBDNF) increased, while that of mature BDNF (mBDNF), cAMP-response element binding protein (CREB) and phosphorylated CREB (pCREB) decreased compared with the control group. Furthermore, the expression of BACE1 protein and tau phosphorylation level in PFC and hippocampus increased, APP mRNAs in PFC and prolonged induction of BACE1 in hippocampus. Our results show that chronic Pb exposure from pubertal stage onward can either initiate divergent synaptic-related gene expression patterns in adulthood or trigger time-course of neurodegenerative profile within the PFC or hippocampus, which can contribute consistent deficits of cognition across subsequent age-nodes.
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Materials Today Communications
·
2020
·
IF 2.678
Qi Wang,Xiaocui Cheng,Huanhuan Li,Fangfang Yu,Qiuyue Wang,Meixia Yu,Dongdong Liu,Juan Xia
A novel self-assembled core-shell structure fluorescent probe (DNA-QDs/AuNPs, DQA) was constructed for the first time by integrating gold nanoparticles (AuNPs) and DNA quantum dots (DNA-QDs), which can combine with the immunomagnetic separation (IMS) to test for the levels of Salmonella typhimurium (S. typhi). Based on the polyethyleneimine (PEI)-coated AuNPs, the DQA probe can simplify the tedious process of modifying the probe by aptamers and amplify the fluorescence signal by electrostatic adsorption of a large amount of DNA-QDs. The S. typhi can be successfully detected with a broad range from 10 cfu mL −1 to 1.0 × 10 7 cfu mL -1 with the limit detection (LOD) of 13.6 cfu mL −1 . Moreover, the obtained DQA probe exhibits high specificity and stability detection. Due to the convenient and rapid synthesis of the DQA probe, it will be suitable for ordinary technicians in the detecting of S. typhi conveniently and effectively.
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APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2020
·Vol. 191
·期 3
·1340-1352
·
IF 2.277
Su Yanbin,Sun Chengyu,Sun Xuwei,Wu Ruixue,Zhang Xing,Tu Yunzhou
The aim of this study was to explore whether or not acetylresveratrol as a potential substitute for resveratrol dragged the toxic aldehyde to inhibit the mutation of mitochondrial DNA. The results revealed that the acetylresveratrol shifted ultraviolet peak of trans -crotonaldehyde from 316 to 311 nm. In mitochondria, the acetylresveratrol split the ultraviolet peak at 311 nm of trans -crotonaldehyde into 311 nm and 309 nm; the aldehyde Raman band of trans -crotonaldehyde was red shifted by the acetylresveratrol from 1689 to 1686 cm −1 with obvious band decline; Raman bands at 1149 cm −1 , 1168 cm −1 , and 1325 cm −1 of acetylresveratrol disappeared. In aldehyde dehydrogenase, the aldehyde Raman band of trans- crotonaldehyde was red shifted by the acetylresveratrol from 1689 to 1684 cm −1 with band decline; Raman bands at 1150 cm −1 , 1168 cm −1 , and 1324 cm −1 of acetylresveratrol declined. The weak acidic microenvironment was the best, for the acetylresveratrol dragged the toxic aldehyde of trans -crotonaldehyde. Compared with the resveratrol, the effect of the acetylresveratrol on the toxic aldehyde of trans -crotonaldehyde was very similar to that of the resveratrol. The acetylresveratrol is very suitable as a potential substitute for resveratrol dragged the toxic aldehyde to inhibit the mutation of mitochondrial DNA.
In mitochondria, the Raman band of the toxic –CH=O of trans- crotonaldehyde (TCA) dragged by the Acetyl-Res from 1689 to 1686 cm −1 with obvious band decline, while the Raman bands at 1149 cm −1 , 1168 cm −1 , and 1325 cm −1 of the Acetyl-Res disappeared, respectively. The Acetyl-Res is very suitable as a potential substitute, for the Res dragged the toxic –CH=O of TCA to inhibit the mutation of mitochondrial DNA for anticancer.
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BIOSENSORS & BIOELECTRONICS
·
2021
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IF 10.618
Jia Ding,Yunlei Zhou,Qian Wang,Shiyun Ai
As an important epigenetic modification , 5-hydroxymethylcytosine (5hmC) aroused wide concern about the distribution and the function. Due to the necessity of 5hmC detection, a novel photoelectrochemical (PEC) biosensor was established based on the in-situ generated heterojunction of Bi 4 NbO 8 [email protected] 2 S 3 , which was employed as the substrate material with excellent photoelectric property . The specific recognition of 5hmC relied on the covalent reaction between –CH 2 OH of 5hmC and –SH on the substrate electrode under the catalysis of M.HhaI methyltransferase . Afterwards, ZrO 2 was used as signal amplification unit capturing by the specific reaction of Zr with the phosphate group of 5hmC. The experimental results demonstrated well specificity and sensitivity of this biosensor. Under optimal conditions, the linear relationship between the photocurrent and the logarithm value of 5hmC concentration was constructed with the range from 0.3 to 300 nM and the detection limit of 0.0779 nM (S/N = 3). The procedures of constructing this biosensor were compact and convenient, and this biosensor realized actual detection of 5hmC level in wheat sample. Significantly, this biosensor was applied to a preliminary study that the heavy metal Pb 2+ and the perfluorooctanoic acid influence the expression of 5hmC in the genomic DNA of wheat seedling roots and leaves.
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MATERIALS TECHNOLOGY
·
2021
·
IF 3.846
Jiyao Zheng,Ya Di,Liming Gao,Xianglei Kong,Yue Zheng,Junling Han,Jidong Wang
Carcinoembryonic antigen (CEA) is usually utilised as a reliable diagnostic and predictive marker of gastric cancer (GC). Herein, a target-responsive, label-free aptamer ‘signal-on’ fluorescent biosensing system was developed based on the synthesis of DNA-templated quantum dots via one-pot route and interactions with graphene oxide. CEA triggered aptamer-DNA hairpins hybridisation to release DNA to hybridise with DNA-CdTe QDs on GO and led to the fluorescence recovery of system with a ‘signal-on’ status. The fluorescence intensity was proportionate to CEA concentration, and highly sensitive sensing platform for detecting CEA was achieved with a wide linear range (5–250 ng/mL) and a low detection limit (1.17 ng/mL). This sensing strategy, the targeted detection of aptamer for CEA, provided a new direction in the detection of protein and construction of fluorescence biosensors.
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ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Yuxia Yang,Yue Tang,Chunxiao Wang,Bangyan Liu,Yuangen Wu
Pyrethroid pesticides residues will not only pollute the environment, but also cause high toxicity to the human body. It is significant to establish an efficient and accurate method for pyrethroid detection in food. Considering that the common biomolecules like antibody is complicated and easy to inactivate, it is urgent to find a new type of biomolecule to specifically recognize pyrethroid pesticides. This study proposed the Capture-SELEX strategy to firstly select λ-cyhalothrin aptamer by immobilizing random ssDNA library. High-throughput sequencing was performed on the enriched ssDNA library through multiple Capture-SELEX rounds. Comprehensively inspecting structural similarity and homology , six sequences were chosen from five families for further analysis. The results showed that the aptamer (named LCT-1) could specifically recognize λ-cyhalothrin with the strongest affinity ( K d = 50.64 ± 4.33 nmol L −1 ). Molecular docking results revealed that the binding sites between λ-cyhalothrin and LCT-1 aptamer are mainly related to the bases A-5, C-6, C-28, A-29, C-30, G-31 and G-32. The LCT-1 aptamer was truncated to a shorter sequence (named as LCT-1-39) by removing other irrelevant bases, and its K d value was determined as (10.27 ± 1.33) nmol·L −1 by Microscale Thermophoresis (MST). Both LCT-1 and LCT-1-39 aptamers were employed as recognition molecules to establish the colorimetric aptasensors for λ-cyhalothrin detection, which displayed good repeatability and reproducibility. The detection limit of the aptasensors were individually calculated as 0.0197 μg ml −1 and 0.0186 μg ml −1 , and their recovery rate of λ-cyhalothrin in pear and cucumber samples was in the range of 82.93–95.50%. This article provides a promising application for the detection of λ-cyhalothrin.
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危险品化学品经营许可证（带存储）