MATERIALS LETTERS
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2017
·
IF 2.572
Shenghong Yang,Xiaohan Sun,Yu Chen
The rapid development of fluorescent nanomaterials has broadened a new horizon for the design of easy-to-use and sensitive sensors. Herein, the stable, water-soluble and fluorescent L-Cystine modified copper nanoclusters (CuNCs) were prepared via size-focusing etching method. The maximum emission wavelength of the obtained CuNCs was 500 nm with an excitation wavelength of 396 nm. The as-prepared CuNCs can only emit faint light when dispersed in aqueous solution, but generate obvious fluorescence intensity enhancement after adding specific amount of 2,4,6-trinitrotoluene (TNT). Under the optimal conditions, the CuNCs were adopted as an effective fluorescence probe for the detection of trace TNT through fluorescence “turn-on” mode for the first time. The calibration curve of TNT was linear up to 4.8 μM, with a detection limit of 9.1 nM.
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Food Analytical Methods
·
2016
·Vol. 9
·期 12
·3501-3508
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IF 2.167
Shao Yufang,Chen Ying,Liu Jian,Wu Pingping,Ying Yibin,Xie Jing
High-resolution inductively coupled plasma mass spectrometry (ICP-MS) was applied to determine potential toxic element pollution in rice and paddy soil in a lead-acid battery production area. The contamination levels of chromium (Cr), cadmium (Cd), and lead (Pb) in rice and soil at three sampling areas were determined, and the uptake mechanism of Cr, Cd, and Pb from soil to rice grain was preliminarily analyzed. The results show that the range of potential toxic elements in rice concentrations is 3.914–11.788, 0.057–1.215, and 0.187–1.585 mg kg −1 for Cr, Cd, and Pb, respectively. For the paddy soils, Cd concentration is 0.610 mg kg −1 , which exceeds 0.300 mg kg −1 , a value recommended by Chinese Environmental Quality. Bioaccumulation factor (BAF, an index of the ability of plant to absorb a particular metal with respect to its concentration in the soil substrate) values of Cr, Cd, and Pb were less than 1 in all rice samples, suggesting that the bioavailability of these metals was relatively low in rice. The correlations between BAF values with organic matter (OM) and pH were calculated to find the mobility and availability of potential toxic elements in soil. At last, the estimated daily intake (EDI) dose was determined to evaluate the health risk to local population via consumption of rice contaminated with Cr, Cd, and Pb. This study indicated that the accumulation and translocation of potential toxic elements from soil to rice could cause the health risk for the local population.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2016
·
IF 2.857
Yu-Chun Wang,Wen-Jun Qian,Na-Na Li,Xin-Yuan Hao,Lu Wang,Bin Xiao,Xin-Chao Wang,Ya-Jun Yang
Tea plant (Camellia sinensis) is one of the most economically valuable crops in the world. Anthracnose can affect the growth of leaves and cause serious yield losses of tea. Tea plants are rich in secondary metabolites; however, their roles in resistance to anthracnose are unclear. Herein we compared the contents of total phenolics, catechins, and caffeine in two cultivars with different resistances to anthracnose during Colletotrichum fructicola infection. (−)-Epigallocatechin-3-gallate (EGCG), (+)-catechin (C), caffeine, and critical regulatory genes were induced in C. fructicola-resistant tissues. In vitro antifungal tests showed that caffeine more strongly inhibited mycelial growth than tea polyphenols and catechins. Both electron microscopy and bioactivity analysis results showed that caffeine can affect mycelial cell walls and plasma membranes. Through promoter sequences analysis, a number of stress response-related cis-acting elements were identified in S-adenosylmethionine synthetase and tea caffeine synthase. These results demonstrated that (−)-EGCG, (+)-C, and caffeine may be involved in the resistance of tea plants to anthracnose.
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Scientific Reports
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2016
·Vol. 6
·期 1
·1-12
·
IF 5.228
Deng Jun,Wu Sai,Yao Mengyun,Gao Changyou
Chirality is one of the ubiquitous phenomena in biological systems. The left handed (L-) amino acids and right handed (D-) sugars are normally found in proteins and in RNAs and DNAs, respectively. The effect of chiral surfaces at the nanoscale on cellular uptake has, however, not been explored. This study reveals for the first time the molecular chirality on gold nanoparticles (AuNPs) functions as a direct regulator for cellular uptake. Monolayers of 2-mercaptoacetyl-L(D)-valine (L(D)-MAV) and poly(acryloyl-L(D)-valine (L(D)-PAV) chiral molecules were formed on AuNPs surface, respectively. The internalized amount of PAV-AuNPs was several times larger than that of MAV-AuNPs by A549 and HepG2 cells, regardless of the chirality difference. However, the D-PAV-AuNPs were internalized with significantly larger amount than the L-PAV-AuNPs. This chirality-dependent uptake effect is likely attributed to the preferable interaction between the L-phospholipid-based cell membrane and the D-enantiomers.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2017
·
IF 2.536
Yusheng Yuan,Liu Yang,Shaopu Liu,Jidong Yang,Hui Zhang,Jingjing Yan,Xiaoli Hu
A sensitive fluorescence sensor for warfarin was proposed via quenching the fluorescence of l -tryptophan due to the interaction between warfarin and l -tryptophan. Warfarin, as one of the most effective anticoagulants , was designed and synthesized via lipase from porcine pancreas (PPL) as a biocatalyst to catalyze the Michael addition of 4-hydroxycoumarin to α, β-unsaturated enones in organic medium in the presence of water. Furthermore, the spectrofluorometry was used to detect the concentration of warfarin with a linear range and detection limit (3σ/k) of 0.04–12.0 μmol L − 1 (R 2 = 0.994) and 0.01 μmol L − 1 , respectively. Herein, this was the first application of bio-catalytic synthesis and fluorescence for the determination of warfarin. The proposed method was applied to determine warfarin of the drug in tablets with satisfactory results.
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Advanced Science
·
2024
·
IF 14.3
Dingyuan Guo,Nan Li,Xiaoyan Zhang,Runxin Zhou,Jie He,Xiao-Ping Ding,Weixing Yu,Fuqiang Tong,Sibi Yin,Yu Wang,Xin Xu,Long Wang,Mingzhu Fan,Shan Feng,Ke Liu,Ke Tang,Zhuqing Ouyang,Yusong R Guo,Yugang Wang
N 6 -acetyl- L - lysine residue is abundant in dietary protein but little is known about its potential influences on the diet-consumers. Herein, it is reported that Lysyl-tRNA synthetase (KARS) mediates co-translational deposition of diet-derived N 6 -acetyl- L -lysine (AcK) in nascent proteins to contribute to the acetylome in cells. Acetylated dietary protein is a direct source of AcK that can widely and substantially regulate the acetylome in multiple organs of mice. By analyzing the mechanisms underlying AcK contributing to the acetylome in mammalian cells, it is found that KARS can utilize AcK as an alternative substrate to produce N 6 -acetyl- l -lysyl-tRNA. The crystal structure of KARS in complex with AcK at 2.26 Å resolution shows that AcK shares the same substrate-binding pocket as L -lysine, allowed by a sidechain flip of Tyr499. The generated N 6 -acetyl- L -lysyl-tRNA introduces AcK into growing nascent polypeptide and results in protein acetylation, including the regions buried inside folded proteins that are post-translational modification (PTM)-inaccessible and functionally important. This undocumented protein modification mechanism is inherently different from PTM and termed as co-translational modification (coTM). It is expected to extend the repertoire of acetylome and improve the understanding of protein modification mechanisms in cells.
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CELLULOSE
·
2024
·
IF 4.9
Xiang Shuangfei,Cai Dongrong,Ru Hongfu,Ma Yuchen,Xiao Yuanxiang,Zhao Shujun,Fu Feiya,Liu Xiangdong
The rational design of Ag NP decorated cotton fabrics with durable Joule heating performance has attracted great interest. However, the combination of Ag NPs and cotton fabrics is so weak that it hinders its long-term stability in service. Here, an in situ dual-anchoring strategy is employed to enhance the immobilization of Ag NPs by L-Cysteine and DNA on cotton fabrics. Specifically, DNA plays a role as template for immobilizing Ag NPs, while L-Cysteine acts as a binder between cotton fabrics and DNA. Under the synergistic effect of L-Cysteine and DNA, Ag NPs are firmly anchored on cotton fabrics. The obtained cotton fabric based wearable Joule heaters exhibit low electrical resistance (24 Ω/sq) and long-term stability. Furthermore, the cotton fabric based wearable Joule heaters can be heated up to 60.3℃ at a low driving voltage of 4.5 V, and maintain superior Joule heating performance even after 1000 times of bending fatigue tests. Thus, this work holds great promise for a durable wearable Joule heater. Graphical abstract
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TALANTA
·
2024
·
IF 5.6
Haibo Chen,Yan Luo,Wenrong Cai,Laidi Xu,Junyao Li,Yong Kong
Au nanoparticles (AuNPs) are decorated by l -cysteine (L-Cys), and the resultant chiral L-Cys/AuNPs can be used for colorimetric discrimination and spectroscopic detection of the tyrosine (Tyr) enantiomers . Melamine (Mel) can induce the aggregation of the L-Cys/AuNPs through ligand exchange, leading to a distinct color change from wine red to purple. Owing to the same rotatory direction of L-Cys/AuNPs and L-Tyr, the L-Cys/AuNPs exhibit a significantly higher binding affinity toward L-Tyr than D-Tyr, and thus the Mel induced aggregation of the L-Cys/AuNPs is greatly alleviated by the protection from the L-Tyr protective layer. Therefore, the Tyr enantiomers can be simply discriminated by naked eyes. In addition, the absorbance of the aggregated L-Cys/AuNPs at ∼630 nm increases linearly with decreasing concentrations of L-Tyr ranging from 10 nM to 1 mM due to the weakened protection effect from L-Tyr, and thus spectroscopic detection of L-Tyr can also be accomplished by the developed L-Cys/AuNPs with a limit of detection (LOD) of 5.3 nM.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Wenzhuang Cui,Chu Gong,Yujie Liu,Ying Yue,Jun Wang,Zhizhou Yang,Junli Yang
Antibacterial hydrogels as burn wound dressings are capable of efficaciously defending against bacterial infection and accelerating burn wound healing. Thus far, a large plethora of antibacterial hydrogels have adopted numerous components and intricate preparation processes, yet restricting their practical industrialization applications. Simple and effective preparation methods of antibacterial hydrogels are hence urgently needed. Herein, an easy but efficacious strategy with the employment of two natural products pullulan and ε-poly- l -lysine (ε-PL) was designed to fabricate composite antibacterial hydrogels for burn wound healing for the first time. The hydrogel crosslinking networks were formed through amidation reactions between carboxylated pullulan derivative (CP) and ε-poly- l -lysine hydrochloride (ε-PL·HCl). The resulting hydrogels possessed high transparency, porous structures, tunable gelation time and gel content, relatively low swelling ratios, appropriate self-degradability, proper mechanical properties, strong in vitro bacteriostatic activities, non-cytotoxicity, capacities of facilitating cell migration and excellent hemocompatibility. In the infected burn model of mice, the hydrogels were observed to display prominent in vivo antibacterial activities and enable the acceleration of burn wound healing. We opine the simply and effectively prepared antibacterial hydrogels as promising dressings for burn wound recovery have broad industrialization prospects.
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JOURNAL OF FOOD SCIENCE
·
2017
·Vol. 82
·期 12
·2901-2909
·
IF 1.815
Ruixue Guo,Xinbo Guo,Xiaodan Hu,Arshad Mehmood Abbasi,Lin Zhou,Tong Li,Xiong Fu,Rui Hai Liu
The purpose of this work was to improve the oral bioavailability of a poorly soluble functional food ingredient, the total flavones of Hippophaë rhamnoides L. (TFH). A self-microemulsion drug delivery system (SMEDDS) was developed to overcome the problems of poor absorption of TFH in vivo . The optimal SMEDDS significantly enhanced the solubility of TFH up to 530 times compared to that in water. The mean droplet size was 61.76 nm with uniform distribution. And the loaded system was stable at 25 °C for 3 mo with transparent appearance. The in vitro release of TFH from SMEDDS was faster and more complete than that from suspension. After oral administration of TFH–SMEDDS in rats, the relative bioavailability of TFH was dramatically improved for 3.09 times compared with the unencapsulated form. The investigation indicated the potential application of SMEDDS as a vehicle to improve the oral bioavailability of TFH. Practical Application The lipid-based nanotechnology, namely self-microemulsion drug delivery system (SMEDDS) was used to improve the bioavailability and oral delivery of total flavones of Hippophaë rhamnoides L. (TFH). The relevant bioavailability of TFH could be remarkably 3-fold improved by the optimized SMEDDS. The SMEDDS produced via a simple one-step process for poorly soluble TFH to achieve a significant improvement in the bioavailability, may endorse the promising utilization of TFH in functional foods as well as pharmaceutical fields with an enhanced absorption in vivo .
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ANALYTICAL SCIENCES
·
2017
·Vol. 33
·期 8
·917-923
·
IF 1.228
Gangbing ZHU,Heng SUN,Junjuan QIAN,Xiangyang WU,Yinhui YI
The simultaneous and sensitive electrochemical detection of dihydroxybenzene isomers (hydroquinone, HQ; catechol, CC; resorcinol, RS) is of great significance because such isomers can be awfully harmful to the environment and human health. In this paper, by preparing poly(L-arginine) modified glassy carbon electrode (P-L-Arg/GCE) with a simple method, a highly sensitive electrochemical sensor for simultaneously detecting HQ, CC and RS was constructed successfully due to the large surface area, good electronic properties and catalytic ability of P-L-Arg/GCE and the electrostatic action between P-L-Arg (positive) and targets (negative). Under the optimized conditions, the results show that the P-L-Arg/GCE has a wide linear range from 0.1 to 110.0 μM for HQ ,CC and RS. The detection limits for HQ, CC and RS are 0.01, 0.03 and 0.1 μM, respectively. Finally, the proposed sensor was successfully applied in real sample analysis.
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ChemSusChem
·
2017
·Vol. 10
·期 8
·1669-1674
·
IF 7.226
Zhongsen Ma,Hualei Hu,Zhongqiang Sun,Wenting Fang,Jian Zhang,Longfei Yang,Yajie Zhang,Lei Wang
Graphical Zeolite catalysis : Zeolite L is synthesized by the hydrothermal method, post-treated by NH 4 exchange to adjust its acidity, and characterized by various techniques. In the dehydration of fructose catalyzed by modified zeolite L in the ionic liquid [bmim]Br, 99.1 % yield of 5-hydroxymethylfurfural (HMF) is obtained. Zeolite L was synthesized by the hydrothermal method and post-treated by NH 4 exchange to adjust its acidity. The samples were systematic characterized by various techniques including XRD, X-ray fluorescence spectroscopy, N 2 adsorption–desorption, scanning electron microscopy, pyridine IR spectroscopy, and NH 3 temperature-programmed desorption. The results demonstrated that the NH 4 -exchange post-treatment increased the surface area, micropore volume, and acidity of zeolite L. The catalytic performance of the samples was tested in the dehydration of fructose to 5-hydroxymethylfurfural (HMF) in ionic liquid (1-butyl-3-methylimidazolium bromide, [bmim]Br). 99.1 % yield of HMF was obtained when the KL-80 °C-1 h sample (KL zeolite treated with 1 m NH 4 NO 3 solution at 80 °C for 1 h) was used. The high efficiency could be attributed to the appropriate acid properties of the catalyst. The zeolite catalyst could be reused four times without significant decrease in activity.
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