CHEMICAL ENGINEERING JOURNAL
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2021
·
IF 13.273
Minghui Wang,Yuxin Lin,Jianyang Lu,Zhaowei Sun,Ying Deng,Lei Wang,Yongxiang Yi,Jinlong Li,Jie Yang,Genxi Li
The ongoing outbreak of coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has highlighted that new diagnosis technologies are crucial for controlling the spread of the disease. Especially in the resources-limit region, conveniently operated detection methods such as “naked-eye” detection are urgently required that no instrument is needed. Herein, we have designed a novel and facile strategy to fabricate covalent organic framework (COF) capsules, which can be utilized to establish a new colorimetric assay for naked-eye detection of SARS-CoV-2 RNA. Specifically, we employ the digestible ZIF-90 as the sacrificial template to prepare the hollow COF capsules for horseradish peroxidase (HRP) encapsulation. The fabricated COF capsules can provide an appropriate microenvironment for the enzyme molecules, which may improve the conformational freedom of enzymes, enhance the mass transfer, and endow the enzyme with high environmental resistance. With such design, the proposed assay exhibits outstanding analytical performance for the detection of SARS-CoV-2 RNA in the linear range from 5 pM to 50 nM with a detection limit of 0.28 pM which can go parallel to qTR-PCR analysis. Our method also possesses excellent selectivity and reproducibility. Moreover, this method can also be served to analyze the clinical samples, and can successfully differentiate COVID-19 patients from healthy people, suggesting the promising potential in clinical diagnosis.
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CHEMOSPHERE
·
2021
·
IF 7.086
Fang Zhu,Siyi Zhou,Mengying Sun,Jianfeng Ma,Wei Zhang,Kunjie Li,Hao Cheng,Sridhar Komarneni
Mg doped Ni(OH) 2 was synthesized and investigated as an efficient material to activate persulfate (PS) for phenol degradation. The property of the Ni(OH) 2 material was enhanced by Mg doping as the removal efficiency of phenol was increased from 74.82 % in Ni(OH) 2 /PS system to 89.53 % in Mg-doped Ni(OH) 2 /PS system within 20 min. Such a high removal efficiency revealed that doping Mg into Ni(OH) 2 brings about more defects (oxygen vacancies), which facilitated the formation of more active species in the degradation process. The removal efficiencies of phenol increased with the increase of the initial pH from 3 to 11. The influences of Cl − , NO 3 − and HCO 3 − on the stability of the system were also studied and the results showed that removal rates of all systems in the presence of these different inorganic anions could reached about 90 % within 20 min. Based on the electron spin resonance (ESR) experiments, 1 O 2 , O 2 ·- , ·OH and SO 4 •− were identified as the active species in Mg-doped Ni(OH) 2 /PS system for phenol degradation and a degradation mechanism was proposed for this system. In addition, the as-prepared material retained its activation performance even after 3 repeated cycles.
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CATALYSIS LETTERS
·
2021
·Vol. 152
·期 5
·1495-1505
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IF 3.186
Zhang Yue,Wang Li,Ma Qingqing,Wang Nan,Pan Zhiyong
SBA-15 was modified via post-treatment with (NH 4 ) 2 SiF 6 and (NH 4 ) 3 AlF 6 solution (NSF-T and NAF-T) and applied as support of Pd catalysts for the hydrogenation of 2-ethyl-anthraquinone (EAQ). The characterization results revealed that F could be incorporated into the framework of SBA-15 by forming Si–F bond and the amount of F increased as the molar ratio of F in treating solution to Si species in SBA-15 (F/Si) increased from 0.1 to 0.4 for NSF-T and 0.3 to 0.6 for NAF-T. At the same F/Si, the incorporated amount of F was larger with NSF-T than with NAF-T, and the latter could simultaneously create acid sites in catalysts. The hydrophobicity of catalysts could be effectively improved by incorporating F into SBA-15 supports, which favored the catalytic performance. The catalyst on SBA-15 treated by NSF with F/Si of 0.3 exhibited the high initial activity (0.24 molH 2 ·gPd −1 ·min −1 ) and selectivity (95.5%). Graphic Abstract
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Molecular Catalysis
·
2021
·
IF 5.062
Wei Luo,Jiagui Hu,Jiapeng Lu,Huili Zhang,Xiaoping Wang,Yuantao Liu,Liqing Dong,Xiaobin Yu
ω-transaminase can mediate the asymmetric synthesis of chiral amines from aldehydes and ketones , which has important value in the synthesis of pharmaceutical intermediates. A novel ω-transaminase derived from Paracoccus pantotrophus ( Pp TA) was obtained and cloned in E. coli BL21(DE3) for expression. The enzyme has high activity for 2-ketobutyric acid and benzylamine , as well as for aromatic compounds with side chains longer than ethyl aliphatic hydrocarbons . Molecular simulation showed that the S-pocket in the active center is larger than those of other ω-transaminases. Thereafter, a whole-cell catalytic system was designed to prepare l -2-aminobutyric acid by cascading Pp TA and other enzymes. By using several strategies (regulation of RBS intensity, by-product decomposition and cofactor self-sufficiency), whole-cell cascade biocatalysis showed a high ee value (> 99%) and high yield (71%) in one pot reaction . This study therefore proposes an efficient biocatalyst for the synthesis of unnatural amino acids with the participation of ω-transaminase.
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SENSORS
·
2019
·Vol. 19
·期 22
·5055
·
IF 3.031
Min Jiang,Peng Sun,Jie Zhao,Lihua Huo,Guofeng Cui
Flexible physiological medical devices have gradually spread to the lives of people, especially the elderly. Here, a flexible integrated sensor based on Au nanoparticle modified copper hydroxide nanograss arrays on flexible carbon fiber cloth ([email protected](OH)2/CFC) is fabricated by a facile electrochemical method. The sensor possesses ultrahigh sensitivity of 7.35 mA mM−1cm−2in the linear concentration range of 0.10 to 3.30 mM and an ultralow detection limit down to 26.97 nM. The fantastic sensing properties can be ascribed to the collective effect of the superior electrochemical catalytic activity of nanograss arrays with dramatically enhanced electrochemically active surface area as well as mass transfer ability when modified with Au and intimate contact between the active material ([email protected](OH)2) and current collector (CFC), concurrently supplying good conductivity for electron/ion transport during glucose biosensing. Furthermore, the device also exhibits excellent anti-interference and stability for glucose detection. Owing to the distinguished performances, the novel sensor shows extreme reliability for practical glucose testing in human serum and juice samples. Significantly, these unique properties and the soft structure of silk fabric can provide a promising structure design for a flexible micro-device and a great potential material candidate of electrochemical glucose sensor.Keywords:Cu(OH)2nanograss;Au nanoparticles;glucose detection;flexible electrochemical biosensor
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BIORESOURCE TECHNOLOGY
·
2019
·
IF 6.669
Zhe Lu,Jingcheng Dai,LingLing Zheng,Zhuoran Teng,Qi Zhang,Dongru Qiu,Lirong Song
Improving carbon availability in astaxanthin production is pivotal in Haematococcus industry. In this study, disodium 2-oxoglutarate (2-OG-2Na) was observed to be a potential carbon regulator to increase the astaxanthin content. To illustrate its efficacy in astaxanthin production, key genes and enzyme were analyzed. Upon 2-OG-2Na treatment, genes ipi , bkt and crtR-b were up regulated, concomitantly, carotenoids and astaxanthin content increased by 15.4% and 14.0% at 120 h, respectively; additionally, Acetyl-CoA carboxylase was activated, consistent with 1.27-fold increase in fatty acids content. PUFAs increased earlier as fatty acids assembly gene fad was up-regulated to 20.56. It was also found that cell division was not compromised. Altogether, it was suggested that increased carbon skeletons were re-directed into the astaxanthin and fatty acids biosynthesis pathway. Furthermore, 2-OG-2Na was applied in ten Haematococcus strains. Of these strains, astaxanthin contents were accelerated with average net increase of 10.48%, exhibiting a scalable paradigm for commercial production.
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Materials Today Communications
·
2020
·
IF 2.678
Tiantian Zhou,Haonan Zhang,Xiaoming Ma,Xin Zhang,Yuanna Zhu,Aiyu Zhang,Yongqiang Cao,Ping Yang
The AgI/Bi 2 MoO 6 /AgBi(MoO 4 ) 2 (ABM) multi-heterostructure nanosheet composite system for visible-light photocatalysis have been successfully constructed by a simple one-step calcination method. The ABM multi-heterostructure composite photocatalysts exhibited the obviously improved photocatalytic performance than the pristine Bi 2 MoO 6 , AgBi(MoO 4 ) 2 , and AgI for the degradation of both Tetracycline hydrochloride (TC) and Rhodamine B (RhB). The apparent degradation rate for the ABM sample with the highest photocatalytic activity is 5.4 (TC) and 23.4 times (RhB) that of Bi 2 MoO 6 , 4.0 (TC) and 4.9 times (RhB) that of AgBi(MoO 4 ) 2 , and 1.9 (TC) and 2.4 times (RhB) that of AgI, respectively. This ABM sample with the highest activity showed a Bi 2 MoO 6 /AgBi(MoO 4 ) 2 composite nanosheet morphology (lateral size: 1–5 u m, thickness: 90−130 nm) with a large amounts of small AgI nanocrystals (diameter: 2−25 nm) uniformly distributed on the surface of composite sheet. The outstanding performance of the ABM composite photocatalysts should be ascribed to the effective separation of photoinduced electron-hole pairs via the multi-heterostructure in ABM, the enhanced visible-light absorption, as well as the two-dimensional (2D) morphology benefitting to the exposure of plenty of surface reactive sites and significant reduction of migration distances of photogenerated carriers. It is hoped that this facile method for the fabrication of multi-heterostructure in our work could promote and inspire the other multicomponent functional nanomaterials used in the fields of not only photocatalysis, but also solar cells, sensors, catalysis, and so on, in the future.
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ChemElectroChem
·
2019
·Vol. 6
·期 20
·5313-5320
·
IF 3.975
Jingting Chen,Suqin Ci,Genxiang Wang,Nangan Senthilkumar,Mengtian Zhang,Qiuhua Xu,Zhenhai Wen
Graphical In nanotechnology we trust : A high-performance acid-alkali asymmetric-electrolyte electrolyzer (AAAE) was set up to implement energy-saving H 2 generation thanks to nanotechnology advances that enable us to develop self-supported electrodes with Ni(OH) 2 nanosheets for high-activity catalysis of urea oxidation. It is highly attractive to develop efficient water electrolysis route to implement hydrogen generation in a low-cost way, which while requires excess energy to surmount the activation barriers majorly limited by oxygen evolution reaction at anode side. Herein, we report an acid-alkali asymmetric-electrolyte electrolyzer (AAAE) that can achieve substantial energy saving for electrolysis hydrogen generation, thanks to the assistance of electrochemical neutralization energy (ENE) and urea oxidation reaction (UOR). To this end, free-standing Ni(OH) 2 nanosheets on carbon cloth (Ni(OH) 2 −NSs/CC) is fabricated as catalytic anode for alkaline UOR, which manifests impressively high catalytic activity with delivering 10 mA cm −2 at 1.32 V and long-term durability. The as-developed AAAE only requires an applied voltage of 0.66 V to release the electrolysis current of 10 mA cm −2 , yielding the H 2 Faraday efficiency close to 100 % along with H 2 production rate of 183.8 μmol h −1 .
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Frontiers in Pharmacology
·
2019
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IF 3.845
Lin Xiao-mian,Luo Wu,Wang Heng,Li Rong-zhen,Huang Yi-shan,Chen Lian-kuai,Wu Xiao-ping
The prostaglandin-endoperoxide synthase-2 (PTGS2) plays essential roles in diverse pathological process. Although recent studies implied that PTGS2 was closely related with chemoresistance, the precise roles and the underlying mechanisms of PTGS2 in the developing process of chemoresistance in non-small cell lung cancer (NSCLC) remained elusive. In the present study, we revealed a novel molecular mechanism of PTGS2 implicated in the chemoresistance of NSCLC and proposed a model for the positive feedback regulation of PTGS2 in the process of developing resistance phenotype in NSCLC cells. Our results demonstrated that cisplatin induced PTGS2 expression through the ROS-ERK1/2-NF-κB signaling axis. The prostaglandin E2 (PGE2) derived from PTGS2 catalyzation further strengthened PTGS2 expression via the PGE2-EPs-ERK1/2 positive feedback loop, which induced multidrug resistance of NSCLC cells through up-regulation of BCL2 expression and the subsequent attenuation of cell apoptosis. Consistently, high levels of both PTGS2 and BCL2 were closely associated with poor survival in NSCLC patients. Inhibition of PTGS2 significantly reversed the chemoresistance in the resistant NSCLC in vitro and in vivo. Our results suggested that PTGS2 might be employed as an adjunctive therapeutic target for improving the response to the therapeutic agents in a subset of resistant NSCLC.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2019
·
IF 2.606
Xia Yan,Shuqing Cao,Ying Li,Pei Xiao,Zipeng Huang,Haiyan Li,Yan Ma
Curcumin (Cur) and its nanomedicine were researched in Caco-2 cells to investigate the internalization and subcellular transport across Caco-2 cell monolayer membrane. Cur loaded polymer micelle (PM), nanoemulsion (NE) and liposome (Lipo) were prepared to explore the transport process incorporating endocytosis, intracellular trafficking and transcytosis in Caco-2 cells. Firstly, nanocarriers could contribute to the cellular uptake and improve the stability of Cur within the Caco-2 cells. The co-mediation of clathrin , lipid raft/caveolae and/or macropinocytosis were involved in the internalization of Cur nanopreparations, indicating the diversity and partial energy-dependency of the endocytosis. Secondly, the endocytosed nanocarriers with Cur successively transported from apical early endosome to late endosome with the extension of incubation time . Furthermore, the intracellular location of Cur nanomedicines in lysosome and endoplasmic reticulum (ER) were detected and illustrated the actual transport of Cur nanomedicine to lysosome and ER. Finally, nanocarriers could enhance the transcytosis of Cur, involving the energy-dependent patterns. Especially, Cur-PM exhibited more advantages in regulating and coordinating the entry and the exit of Cur. The study indicated that the coexistence of multiple transport pathways had the potency to promote nanocarriers transcytosis and provided a rational dosage design for oral Cur.
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AICHE JOURNAL
·
2019
·Vol. 65
·期 6
·e16605
·
IF 3.463
Min Xiao,Wenchao Zheng,Helei Liu,Paitoon Tontiwachwuthikul,Zhiwu Liang
1-(2-Hydoxyethyl)-piperidine (1-(2HE)-PP) is a new tertiary amine with desirable properties and can be potentially used to formulate superior absorbents for CO 2 capture. The equilibrium CO 2 solubility of 1-(2HE)-PP solution is measured over temperatures from 298 to 333 K, CO 2 partial pressures from 8.1 to 101.3 kPa and initial amine concentrations from 1 to 5 M. Two thermodynamic models, namely semiempirical model and activity coefficient model are developed for the system. The activity coefficient model shows better estimation solubility with an absolute average relative deviation (AARD) of 7.6%. In the comparison between the two models, a comprehensive analysis is presented. Some suggestions are provided for the similar model development. In addition, the speciation plot of CO 2 loaded 1-(2HE)-PP solution is predicted based on the activity coefficient model. The predictive pH values agree well with experimental data with AARD of 1.0%. Finally, the potential of 1-(2-HE)PP to be an alternative amine in CO 2 capture is evaluated.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2019
·
IF 3.571
Zhongfei Zhang,Puyu Yang,Boyan Gao,Guoren Huang,Man Liu,Liangli Lucy Yu
A novel synthetic route was designed, developed, and utilized to synthesize six high-purity 2-monochloropropanediol fatty acid esters (2-MCPD esters), a group of potential processing-induced food contaminants. A chlorine atom was introduced to C-2 of a diethyl malonate molecule, which was reduced by NaBH4 and followed by esterification using fatty acids. The reaction products were isolated and purified using silica gel columns to obtain three 2-MCPD monoesters and three diesters at about 50–54% and 56–59% yields, respectively. In addition, 2-MCPD monopalmitate and dipalmitate were examined for their acute oral toxicities in Swiss mice. The LD50 values of 2-MCPD mono- and dipalmitate were greater than 5000 mg/kg body weight (BW), along with detectable nephrotoxicity and testicular toxicity. The results of this study may promote future investigation of MCPD ester toxicology and detection.
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