NEW JOURNAL OF CHEMISTRY
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2021
·Vol. 45
·期 19
·8608-8618
·
IF 3.591
Min Chen,Haimei Li,Xueming Su,Runqiang Wu,Huafu Feng,Xiaohang Shi,Jintao Liang,Jiejing Chen,Guiyin Li
Glypican-3 (GPC3) is a potential hepatocellular carcinoma (HCC) serum marker with good sensitivity and specificity. Herein, a label-free electrochemical aptasensor for the detection of GPC3 was developed using a reduced graphene oxide–hemin–chitosan (RGO–H–CS) nanocomposite-modified screen-printed electrode (SPE) as a biosensing platform and GPC3 aptamer as the recognition element. When GPC3 was present, the aptamer could specifically bind with the target GPC3 by specific recognition reaction and form GPC3–aptamer conjugations on the sensing surface, which could alter the electrochemical redox signal of hemin (Fe(III)/hemin(Fe(II))) in the RGO–H–CS nanocomposite recorded by differential pulse voltammetry (DPV). The electrochemical GPC3 aptasensor was characterized via scanning electron microscopy (SEM), Raman spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, currents originating from the oxidation of hemin had a linear relationship with the concentration of GPC3 in the range of 0.01 µg mL−1 to 10.0 µg mL−1 with a detection limit of 7.9 ng mL−1 (S/N = 3). The developed aptasensor was applied to detect the GPC3 level in the human serum samples with the recovery rate of 101.04–104.9%. Moreover, the label-free aptasensor exhibited considerable anti-interfering ability, acceptable stability and good reproducibility. Therefore, this study provides a reliable and objective analysis method to achieve high screening for the early diagnosis of HCC.
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Arabian Journal of Chemistry
·
2021
·
IF 5.165
Aka Alla Martin,Essy Kouadio Fodjo,Zran Vanh Eric-Simon,Zhen Gu,Guangxin Yang,Trokourey Albert,Cong Kong,Hui-Feng Wang
A highly sensitive modified gold (Au) electrode using cysteine-coated silver nanoparticles (Cys-AgNPs) is designed. The electrochemical activity of this designed sensor has exhibited an excellent response for the detection of 3-chloropropane-1,2-diol (3-MCPD). Indeed, the investigation of electrochemical behavior of 3-MCPD at the Cys-AgNPs/Au electrode has shown that the electrocatalytic activity for the 3-MCPD oxidation in NaOH solution is enhanced by three-fold with the presence of Cys-AgNPs film. This Cys-AgNPs film is found to be stable on the Au electrode and the developed electrode can be reused several times while obtaining excellent results. Furthermore, using differential pulse voltammetry technique, the oxidation current peak shows a linear relationship with 3-MCPD concentration between 2.5 ng/mL and 200 ng/mL. The calculated limit of detection (LOD) and limit of quantification (LOQ) are found to be 2.4 ng/mL and 8.6 ng/mL, respectively. A recovery rate ranging from 96% to 105% in the presence of interfering compounds with similar structure to 3-MCPD or not are observed for the determination of 3-MCPD. Moreover, the developed sensor is successfully applied to the determination of 3-MCPD in smoked mackerel and palm oil samples with results which show 3-MCPD concentration ranging from 174 to 439 μg/kg and 895 to 1280 μg/kg in smoked mackerel and palm oil samples, respectively. In addition to the reusability and stability of the electrode, these results suggest that this Cys-AgNPs/Au electrode can be used as an effective sensor to monitor and determine trace level of 3-MCPD in foodstuffs.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2021
·
IF 3.171
Guijia Cui,Hong Wang,Fengping Yu,Haiying Che,Xiaozhen Liao,Linsen Li,Weimin Yang,Zifeng Ma
NASICON-type Na 3 V 2 (PO 4 ) 3 is a promising electrode material for developing advanced sodium-ion batteries. Preparing Na 3 V 2 (PO 4 ) 3 with good performance by a cost-effective and large-scale method is significant for industrial applications. In this work, a porous Na 3 V 2 (PO 4 ) 3 /C cathode material with excellent electrochemical performance is successfully prepared by an agar-gel combined with freeze-drying method. The Na 3 V 2 (PO 4 ) 3 /C cathode displayed specific capacities of 113.4 mAh·g −1 , 107.0 mAh·g −1 and 87.1 mAh·g −1 at 0.1 C, 1 C and 10 C, respectively. For the first time, the 500-mAh soft-packed symmetrical sodium-ion batteries based on Na 3 V 2 (PO 4 ) 3 /C electrodes are successfully fabricated. The 500-mAh symmetrical batteries exhibit outstanding low temperature performance with a capacity retention of 83% at 0 °C owing to the rapid sodium ion migration ability and structural stability of Na 3 V 2 (PO 4 ) 3 /C. Moreover, the thermal runaway features are revealed by accelerating rate calorimetry (ARC) test for the first time. Thermal stability and safety of the symmetrical batteries are demonstrated to be better than lithium-ion batteries and some reported sodium-ion batteries. Our work makes it clear that the soft-packed symmetrical sodium ion batteries based on Na 3 V 2 (PO 4 ) 3 /C have a prospect of practical application in high safety requirement fields.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2021
·
IF 4.464
Cuiwen Jiang,Feiyan Yan,Yuanxin Qin,Jing Liang,Liping Xie,Yanli Wang,Tao Li,Jing Wang,Lufei Zheng,Yu Ya
In this study, a stable and sensitive acetylcholinesterase biosensor was developed for the electrochemical determination of 3-nitropropionic acid (3-NPA). To this end, NaOH etching was used to activate the surface of the glassy carbon electrode, and the morphologies and electrochemistry properties of the resulting electrodes were characterized by various analytical methods. The results indicated dramatic enhancement in the electrochemical properties of electrodes by NaOH etching. As a result, the etched electrodes were applied as novel platforms for the immobilization of acetylcholinesterase. Accordingly, a sensor based on inhibition of 3-NPA toward the activity of acetylcholinesterase was constructed for the electrochemical determination of 3-NPA. Under the optimal conditions, the inhibition rate was found to be proportional to the logarithm of the concentration of 3-NPA from 0.1 to 30 μg/L, and the limit of detection was recorded as 0.05 μg/L. In comparison to conventional detection method, the fabricated biosensor also has added advantages in the form of easy and convenient operation, high sensitivity and better linear range.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 15
·5182-5189
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IF 7.393
Liang Bao,Wenqiang Liu,Yonghua Chen,Yanbo Zhang,Yang Zhang
The local surface plasmon resonance effect induced by metal nanoparticles on perovskites provides a powerful approach to enhance the photoluminescence of such all-inorganic systems. Here, we show that the decoration of CsPb(Br/Cl)3 nanocrystals (NCs) with Au nanoparticles (NPs) leads to a significant enhancement of the blue emission centered at 465 nm, resulting in a photoluminescence quantum yield (PLQY) of up to 73%. Our simulations based on finite difference time domain (FDTD) analysis indicate that the distance between the Au NPs and CsPb(Br/Cl)3 NCs plays a crucial role in determining the local electric field intensity. Our data suggest that an excessive decoration of CsPb(Br/Cl)3 NCs with Au NPs can also reduce the PLQY, as a result of decreased coupling strength.
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ENZYME AND MICROBIAL TECHNOLOGY
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2021
·
IF 3.493
Dong-Xu Jia,Chen-Yi Sun,Yi-Ting Jin,Zhi-Qiang Liu,Yu-Guo Zheng,Mian Li,Hong-Yan Wang,De-Shui Chen
Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g⋅wet cell weight) was selected for investigation. NtDAE showed optimum reaction temperature and pH at 70 °C and 7.0, respectively. In the presence of Co 2+ , its activity was improved by 100.1 %, and the half-life ( t 1/2 ) values reached up to 846.3, 539.2 and 47.8 min at 40, 50 and 60 °C, respectively. Higher k cat / K m values toward d -allulose indicated that the preferred physiological substrate of NtDAE was d -allulose. The conversion yield of 500 g/L d -fructose to d -allulose at 60 °C achieved 29.7 %, and further enhanced to 51.6 % with adding 1.1 M borate. This study provided a useful guidance of further engineering and application of NtDAE for realization of industrial production of d -allulose.
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PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2021
·
IF 5.194
Min Wang,Longji Fang,Tongtong Liu,Xuejie Chen,Yan Zheng,Yilong Zhang,Shiming Chen,Zeng Li
The development of multi-target-directed ligands (MTDLs) may improve complex central nervous system diseases such as Alzheimer's disease (AD). Here, a series of 7-O-1, 2, 3-triazole hesperetin derivatives was evaluated for their inhibition of cholinesterase , anti-neuroinflammatory, and neuroprotective activity. Among the hesperetin derivatives, compound a8 (7-O-((1-(3-chlorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)hesperetin) possessed excellent anti-butyrylcholinesterase activity (IC 50 = 3.08 ± 0.29 μM) and exhibited good anti-neuroinflammatory activity (IC 50 = 2.91 ± 0.47 μM) against NO production through remarkably blocking the NF-κB signaling pathway and inhibiting the phosphorylation of P65. In addition, a8 showed a remarkable neuroprotective effect and lacked neurotoxicity up to 50 μM concentration. Furthermore, possessing significant self-mediated Aβ 1-42 aggregation inhibitory activity, chelated biometals and reduced ROS production were found in compound a8 . In the bi-directional transport assay, a8 exhibited a blood–brain barrier penetrating ability. In this study, the Morris water maze task showed that compound a8 significantly improved the learning and memory impairment of the scopolamine-induced AD mice model. Results highlighted the potential of compound a8 to be a potential MTDL for the development of anti-AD agents.
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PubMed
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2021
·Vol. 69
·期 4
·1428-1437
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IF 2.431
Di Liu,Linbo Gou,Yajun Bai,Tai-Ping Fan,Xiaohui Zheng,Yujie Cai
In this study, the 3-quinuclidinone reductase from Agrobacterium tumefaciens (AtQR) was modified by site-directed mutagenesis. And we further obtained a saturation mutant library in which the residue 197 was mutated. A single-point mutation converted the wild enzyme that originally had no catalytic activity in reduction of ethyl 4-chloroacetoacetate (COBE) into an enzyme with catalytic activity. The results of enzyme activity assays showed that the seven variants could asymmetrically reduce COBE to ethyl ( S )-4-chloro-3-hydroxybutyrate (( S )-CHBE) with NADH as coenzyme. In the library, the variant E197N showed higher catalytic efficiency than others. The E197N was optimally active at pH 6.0 and 40°C, and the catalytic efficiency ( k cat / K m ) for COBE was 51.36 s –1 ·mM –1 . This study showed that the substrate specificity of AtQR could be changed through site-directed mutagenesis at the residue 197.
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PubMed
高影响因子
IONICS
·
2020
·Vol. 26
·期 12
·6049-6056
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IF 2.394
Ren Yaqi,Deng Hao,Zhao Hong,Zhou Zheng,Wei Zhaohuan
A simple and effective method was developed and used to improve the conductivity and density of Li 1.3 Al 0.3 Ti 1.7 (PO 4 ) 3 solid–state electrolyte. The raw powders were synthesized through two different methods to expand the particle size distribution, which could improve the sintered density and the grain boundary connection of the of LATP electrolyte. Through this method, the relative density of the electrolyte made of mixed powders can reach to 97% compared with the 92% and 95% for electrolyte made of single solid-state reaction powder and sol-gel reaction powder, respectively. The conductivity test shows that this electrolyte has high total ion conductivity of 5.23 × 10 −4 S cm −1 at room temperature with the grain boundary conductivity of 6.11 × 10 −4 S cm −1 . With the merits of high density and ion conductivity, this solid-state electrolyte shows good rate capability and cycle performance in aqueous lithium-oxygen batteries.
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ARCHIV DER PHARMAZIE
·
2020
·Vol. 354
·期 2
·2000165
·
IF 2.59
Yushan Huang,Hongmei Hu,Rui Yan,Liwen Lin,Mingxia Song,Xiaodong Yao
A series of 3-phenyl-1-phenylsulfonyl pyrazoles containing an aminoguanidine moiety was designed, synthesized, and evaluated for their antimicrobial and anticancer activities. The majority of the target compounds showed broad-spectrum antimicrobial activity against the tested strains, with minimum inhibitory concentration (MIC) values ranging from 2 to 64 μg/ml. Compound 5k , showing the most potent antimicrobial activity against Bacillus subtilis CMCC 63501 and multidrug-resistant Staphylococcus aureus ATCC 43300 with an MIC value of 2 μg/ml, was the most promising one in this series. It was also effective for S. aureus ATCC 33591 and multidrug-resistant Escherichia coli ATCC BAA-196 at higher concentrations. The bactericidal time–kill kinetics test illustrated that compound 5k had rapid bactericidal potential. Docking results exhibited that compound 5k showed various kinds of binding to the FabH receptor, reflecting that 5k could bind with the active site well. All compounds showed excellent activity against the investigated cancer cells, with IC 50 values ranging from 1.90 to 54.53 µM. Among them, compound 5f showed prominent cytotoxicity with IC 50 = 1.90 µM against A549 cells, while exhibiting lower inhibitory activity against 293T cells (IC 50 = 41.72 µM), indicating that it has the potential for a good therapeutic index as an anticancer drug.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2020
·
IF 4.845
Xia Yuan,Li Liu,Wei Wang,Ya-ru Gao,Die Zhang,Ting-ting Jia,Hai-rong Zeng,Gang Pan,Yi Yuan
The development of small molecule anticancer drugs , with low water solubility and high toxicity, into polymeric prodrugs has developed into a promising strategy in clinical application. In this study, we synthesized a novel G3-C12-mediated esterase-sensitive tumor-targeting polymeric prodrug of camptothecin (CPT), P(OEGMA- co -CPT- co -G3-C12), and explored its anticancer activity against androgen-independent prostate cancer in vitro and in vivo. Compared to free CPT, the multifunctional polymeric prodrug demonstrated improved water solubility and stability, higher intracellular uptake, and enhanced cytotoxicity in DU145 cells in vitro. Furthermore, it displayed an improved accumulation in the tumor and an enhanced anticancer activity in vivo. Hence, P(OEGMA- co -CPT- co -G3-C12) could be a promising drug in the treatment of androgen-independent prostate cancer.
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PubMed
高影响因子
JOURNAL OF RARE EARTHS
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2020
·
IF 3.104
Zhengyu Liu,Jiaming Wu,Xinyu Liu,Wen Wang,Ziwei Li,Ruijing Xu,Yue Ding,Junlian Wang
Rare earths (REs) and Co are critical strategic resources. Their recovery is crucial for ensuring that their supplies are adequate and for reducing environmental pollution. End-of-life NdFeB magnets are important secondary RE sources. The separation of Dy, Nd, and Co from NdFeB leachate using a relatively new extractant (2,3-dimethylbutyl) (2,4,4′-trimethylpentyl)phosphinic acid (INET-3), was investigated in this study. The separation parameters, such as equilibrium pH, phase ratio, INET-3 concentration, and number of counter-current extraction stages, were optimised. The results show that INET-3 can separate Dy, Nd, and Co efficiently. For the preferential extraction of Dy from synthetic iron-free NdFeB leachate, the optimum equilibrium pH is 2.60. Under this condition, separation of Dy from the leachate requires four counter-current extraction stages at A/O (volume ratio of aqueous to organic) = 1:2. This is demonstrated by a simulated four-stage counter-current extraction process. At equilibrium pH = 2.52 and A/O = 1:2, 98% of Dy is recovered with 95% purity. As to the subsequent separation of Nd from the leachate, nearly 100% is recovered with 99% purity through a simulated three-stage counter-current extraction process at equilibrium pH = 5.33 and A/O = 1:1. Approximately 95% of Co remains in the raffinate with 99.98% purity.
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