ChemistrySelect
·
2026
·Vol. 11
·期 7
·e07452
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IF 2.3
Yan Li,Jinhui Zhang,Liang Hao,Hao Zhang
Recent advancements have highlighted the remarkable potential of silver nanoparticles (AgNPs), which are celebrated for their unique properties and effective antimicrobial performance. This study investigates the dual-function potential of carboxylatopillar[5]arene (CP5) as both a reducing and stabilizing agent in the synthesis of AgNPs (CP5-AgNPs). A straightforward synthesis method was developed, demonstrating the effective reduction of silver ions to form stable CP5-AgNPs without the need for additional energy sources. Characterization techniques were utilized to confirm the successful synthesis of CP5-AgNPs, highlighting the simplicity of the preparation method and the ability to control particle size. The incorporation of benzalkonium chloride (BC) onto the surface of CP5-AgNPs through host-guest interactions facilitated the formation of CP5-AgNPs-BC composites, which significantly enhanced their antimicrobial properties. Antibacterial assays demonstrated that CP5-AgNPs-BC exhibited potent synergistic antimicrobial activity against Staphylococcus aureus (ATCC 29213, a common Gram-positive bacterium), with an inhibition zone of ∼1.75 cm and a minimum inhibitory concentration (MIC) of 1.59×10 −3 mM (based on BC), but showed no inhibitory effect on Escherichia coli (ATCC 25922, a common Gram-negative bacterium). The results indicated that the novel CP5-AgNPs-BC composites not only exhibited potent antimicrobial activity against Gram-positive bacteria but also offered a promising platform for the development of advanced drug delivery systems targeting Gram-positive bacterial infections in medical fields. Graphical This work presents a facile synthesis of silver nanoparticles (AgNPs) using carboxylatopillar[5]arene (CP5) as a dual reducing and stabilizing agent. The resulting CP5-AgNPs effectively load benzalkonium chloride (BC) via host-guest interactions, forming composites with enhanced antibacterial activity against Staphylococcus aureus, promising for advanced antimicrobial and drug delivery applications.
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JOURNAL OF MEMBRANE SCIENCE
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2026
·
IF 8.8
Yafei Cheng,Lei Yuan,Xiang'an Yue,Zhijin Su,Jiajie Zheng,Jing Chen,Bin Wu,Xiaocheng Lin
The alkaline electrocatalytic oxidation of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) is a promising route for biomass valorization, yet the coexistence of FDCK (the potassium salt of FDCA) with excess alkali remains a key challenge, making efficient alkali recovery essential. To address this issue, an asymmetric porous cross-linked cation exchange membrane (CEM), featuring a dense ultrathin selective layer supported on a highly porous substrate, was fabricated via a two-step approach: first, preparing a porous base membrane from a chloromethylated/sulfonated polyethersulfone (CMPES/SPES) blend by non-solvent induced phase separation (NIPS); second, functionalizing it through crosslinking with tetraethylenepentamine (TEPA) to form a sub-3 μm selective layer and then post-sulfonation with propane sultone (PS) for charge regulation. The optimal membrane exhibits remarkable diffusion dialysis (DD) alkali recovery performance, achieving a KOH permeability ( U O H − ) of 23.8 × 10 −3 m/h and a KOH/FDCK separation factor ( S O H − / F D C 2 − ) of 55.6. These values represent enhancements by factors of 40–119 and 4–9, respectively, relative to the typical ranges of commercial dense CEMs. Moreover, it maintains stable performance over eight consecutive cycles. This work provides a novel membrane strategy for HMF electrocatalytic oxidation, enabling efficient alkali recovery while preventing valuable FDCA loss, thereby promoting resource efficiency in biomass refining.
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EBioMedicine
·
2026
·
IF 11.2
Lei Zhang,Xiaotong Han,Xinran Gao,Hangyu Chen,Chunliang Shang,Long Chen,Jian Lin,Hongyan Guo
Background Adenomyosis diagnosis and classification relies primarily on magnetic resonance imaging or pathological examination. Liquid biopsy has been widely applied in oncology but remains underexplored for adenomyosis. We aimed to identify liquid-biopsy-derived 5hmC biomarkers to aid adenomyosis diagnosis and molecular subtyping. Methods Genome-wide 5hmC profiles were obtained by 5hmC-Seal from plasma cfDNA (adenomyosis: n = 51; controls: n = 46) and tissue DNA (adenomyosis: n = 26; controls: n = 21). A paired-tissue-driven splitting strategy was used: the training set comprised 26 adenomyosis plasmas with matched tissues plus 23 controls, and the validation set comprised remaining samples. A 5hmC-based logistic regression model was developed and validated. The identified marker amyloid precursor protein ( APP ) was functionally characterised in cellular and mouse models. Findings Ten 5hmC markers were identified by machine learning. The model showed a sensitivity of 0.88 and a specificity of 0.87 (AUC = 0.91) in the validation set, with better performance than CA125 and CA199. 5hmC signatures could distinguish intrinsic from extrinsic adenomyosis subtypes. APP was related to epithelial cell migration and fibrosis in experimental models, which indicated its potential involvement in adenomyosis pathophysiology. Interpretation Plasma cfDNA 5hmC markers show promise for adenomyosis diagnosis and molecular classification. The APP-HOXD9 axis represents a potential therapeutic target warranting further validation. These findings require prospective, multi-centre confirmation before clinical implementation. Funding This work was supported by grant 82274034 from the National Natural Science Foundation of China, grant 2022YFB3604700 from the National Key Research and Development Program of China, and project BYESS2022031 from the Beijing Association for Science and Technology–Young Elite Scientist Sponsorship Program (BAST-YESS).
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高影响因子
ACS Sustainable Chemistry & Engineering
·
2026
·
IF 7.6
Qiuyang Wu,Yimeng Wang,Shuming Jin,Dong Lu,Luo Liu,Fang Wang,Li Deng,Kaili Nie
Abstract HMFO enables O2-dependent conversion of 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) without soluble cofactor regeneration, but prolonged operation is limited by HMF substrate inhibition, pH drift, and enzyme deactivation. Here, these limitations were addressed through a combination of kinetic analysis, enzyme stabilization, and process optimization. High HMF and FDCA concentrations reduced apparent HMFO activity; pH control relieved the FDCA-related acidification effect, whereas HMF caused direct substrate inhibition, favoring controlled feeding over high initial loading. 8B×HMFO was therefore stabilized by computation-guided mutagenesis and disulfide engineering. 8B×HMFO-5M increased apparent melting temperature by 17 °C and extended the 30 °C half-life from 29.1 to 98.5 h while retaining similar apparent kinetic behavior. E. coli resting cells expressing 8B×HMFO-5M served as a simplified catalyst format, and CAT–HMFO fusion improved FDCA formation. Kinetic feeding incorporating HMF substrate inhibition, catalyst deactivation, and a whole-cell effectiveness factor converted 231.5 mM cumulative HMF input to 214.8 mM FDCA in a 3 L stirred-tank reactor over 72 h, giving a 92.8% molar yield. An E-factor of 4.47 kg·kg−1 including crude FDCA isolation demonstrated the material efficiency of this HMFO-based route.
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CHEMICAL ENGINEERING JOURNAL
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2026
·
IF 12.5
Yunfan Gui,Wenzhi Li,Chenchen Gui,Ajibola T. Ogunbiyi,Lida Wang,Guoshun Liu
A reactive benzyl alcohol/5-SSA system enabled efficient corn stover component fractionation. • The fractionation removed 86.52% lignin and 90.51% hemicellulose while retaining 88.45% cellulose. • Low-condensation lignin was recovered through benzyl alcohol-mediated structural protection. • HSQC and DFT revealed that benzyl alcohol suppressed lignin condensation via reactive grafting. • Pretreated residue is converted into high-surface-area porous biochar. Efficient fractionation of lignocellulosic biomass under mild conditions while preserving lignin structural integrity remains a major challenge for biomass valorization. In this work, we developed a binary pretreatment system composed of benzyl alcohol and 5-sulfosalicylic acid (5-SSA) for the mild fractionation and component utilization of corn stover. The results showed that under the optimal conditions (100 °C, 60 min, and 4 wt% 5-SSA), 86.52% of lignin and 90.51% of hemicellulose were removed, while 88.45% of cellulose was retained. The recovered lignin showed high purity, a light color, a low condensation degree, and a relatively uniform molecular-weight distribution. 2D-HSQC NMR combined with DFT calculations suggested that benzyl alcohol may suppress lignin condensation through stabilization of benzylic intermediates and interaction with reactive nucleophilic sites on lignin aromatic rings. In addition, benzyl glycosides were identified as important hemicellulose-derived products in the aqueous phase, suggesting a solvent participated alcoholysis/glycosylation pathway. The cellulose-rich solid residue was upgraded into porous biochar with a specific surface area of up to 1388.13 m 2 g −1 via KHCO 3 -assisted pyrolysis. This work provides an effective strategy for the mild fractionation of corn stover, recovery of high-quality lignin, and integrated valorization of biomass components. Download: Download high-res image (114KB) Download: Download full-size image
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Frontiers in Plant Science
·
2026
·
IF 5.9
Xiaohao Guo,Siyu Lin,Chuan Dong,Xinyu Huang,Hailian Hu,Baohai Li,Yuanyuan Tan,Qingyao Shu
IntroductionSerotonin and melatonin are plant signaling molecules involved in growth, stress responses, and redox balance. While animals produce serotonin via 5‑hydroxytryptophan (5‑HTP), plants are thought to use the tryptamine pathway. Although 5‑HTP exists in plants, its conversion to serotonin lacks a genetically defined enzyme.MethodsWe performed phylogenetic and structural analyses of Arabidopsis aromatic aldehyde synthase (AtAAS), expressed recombinant AtAAS, and examined its 5-HTP decarboxylase activity in vitro. Enzyme kinetic parameters were determined, and endogenous 5-HTP levels in Arabidopsis were quantified. A T-DNA insertion mutant of AtAAS was further used to assess serotonin accumulation following 5-HTP feeding and to determine endogenous levels of serotonin, N-acetylserotonin, and melatonin under normal growth conditions.ResultsAtAAS showed phylogenetic and structural similarities to animal aromatic amino acid decarboxylases and efficiently converted 5-HTP to serotonin in vitro (optimal pH 7.5, 55°C; Km = 1.806 mM, Vmax = 3.089 nmol/min/mg). Endogenous 5-HTP was detectable in Arabidopsis. Compared with wild-type plants, the AtAAS T-DNA insertion mutant exhibited reduced serotonin accumulation following 5-HTP feeding and decreased endogenous levels of downstream N-acetylserotonin and melatonin under normal growth conditions.Discussionhis discovery unveils enzymatic plasticity in plant serotonin biosynthesis and provides evidence for a previously unrecognized 5-HTP-dependent route in Arabidopsis.
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高影响因子
BIORESOURCE TECHNOLOGY
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2026
·
IF 8.2
Junyu Wang,Qianqian Chen,Yuan Wang,Long Su,Zuotao Tan,Dong Liu,Shaohong You,Hongli Wu,Fei Cao
Multi-enzyme cascade valorizes starch-rich TCHRs into 5KGA; • NADP + regeneration built via Ga5DH-SBFA-CAT coupling; • Reverse activity suppressed, enabling efficient GA-to-5KGA conversion; • Fed-batch production of 5KGA reached 203 g/L with TTN of 9.5 × 10 4 . Herbal residues (HRs) are emerging as an important and sustainable biomass resource, driven by the diversification of global health concepts and the rapid growth in related consumption. Herein, we established a valorization route from starch-rich traditional Chinese herb residues (TCHRs) to high-value 5‑keto‑D‑gluconic acid (5KGA) via a multi-enzymatic cascade. Starch-rich TCHRs were first converted to gluconic acid (GA) through enzymatic hydrolysis and oxidation (>80% conversion). Subsequently, an oxidized cofactor regeneration system was established to efficiently convert GA into 5KGA by coupling gluconate-5-dehydrogenase (Ga5DH) with a synthetic bridged-flavin analogue (SBFA), thereby mitigating constraints arising from Ga5DH’s high reductive activity. This system catalyzed the irreversible dehydrogenation of GA to 5KGA, achieving complete conversion of 100 mM GA within 4 h (total turnover number, TTN = 1.0 × 10 4 ). Furthermore, upon scaling up in a 1-L bioreactor with continuous operation for 48 h, 203.3 g/L (950 mM) of 5KGA was produced with a TTN of 9.5 × 10 4 . This value-upgrading strategy provides an efficient and economically viable biotransformation pathway for starch-based non-edible biomass feedstocks. Download: Download high-res image (112KB) Download: Download full-size image
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高影响因子
MICROBIOLOGICAL RESEARCH
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2026
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IF 8.5
Heqi Zhou,Qiuhong You,Routing Wang,Zhixian Lan,Zhou Zhao,Yuchuan Chen,Dekai Zheng,Kaikai Zhang,Jian Sun
Liver injury and persistent inflammation are common processes underlying liver disease pathogenesis, ultimately leading to fibrosis/cirrhosis and carcinoma. Clostridium sporogenes , a commensal gut bacterium, can produce bioactive metabolites to inhibit colon inflammation and promote neuronal repair. However, there is little information on how C. sporogenes affects injury and inflammation in the liver. Here, we found that live C. sporogenes supplementation significantly attenuates hepatic injury across multiple animal models. Including acute and chronic carbon tetrachloride (CCl 4 ) induced liver damage, as well as methionine-choline-deficient (MCD) diet-induced steatohepatitis. Metabolomics analysis in the supernatants and fecal sample of mice treated with C. sporogenes revealed that live C. sporogenes was able to generate a vital bioactive molecule 5-aminovaleric acid (5-AVA). 5-AVA exerted protective effects against liver damage in both acute and chronic liver models. Furthermore, both C. sporogenes and 5-AVA significantly prevented LPS-induced M1 macrophage activation in vivo and in vitro . The hepatoprotective effects of C. sporogenes in CCl 4 -exposed mice were abolished with macrophage depletion. Mechanistically, 5-AVA upregulated interferon-induced protein with tetratricopeptide repeats 1 (IFIT1), which inhibited NF-κB pathway activation, suppressed pro-inflammatory cytokine expression, and attenuated liver inflammation. These findings demonstrate that C. sporogenes and 5-AVA ameliorate hepatic injury by suppressing M1 macrophage activation via the IFIT1-NF-κB signaling pathway, highlighting their potential as novel therapeutic candidates after successful translation into clinical applications. Synopsis Clostridium sporogenes alleviates acute and chronic liver injury and inflammation through its metabolite 5-aminovaleric acid (5-AVA). 5-AVA suppresses M1 macrophage activation by upregulating IFIT1, inhibiting the NF-κB pathway, and exerting hepatoprotective effects.
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高影响因子
INORGANIC CHEMISTRY
·
2025
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IF 4.7
Qitao Wu,Zhenzhen Lin,Yuzhi Zou,Wenxin Huang,Yanshi Li,Xiaosen Huang,Jiankai Wang,Javokhir Toshov,Sardor Donaev,Hengcong Tao,Shunli Li
Electrocatalytic oxidation of 5-hydroxymethylfurfural to prepare high-value-added chemicals is an effective solution for a sustainable alternative to fossil fuels. We report a novel one-step hydrothermal sulfurization strategy to construct hierarchical FeNi-S@NF nanoflower architectures comprising interconnected 2D nanosheets. This catalyst design synergistically integrates high conductivity (i.e., nickel foam (NF) substrate), abundant active sites (i.e., 2D nanosheet), and optimized electronic structure (i.e., sulfur doping), achieving exceptional electrocatalytic 5-hydroxymethylfurfural (HMF) to 2,5-furandicarboxylic acid (FDCA) conversion. By optimizing the classical two-step hydrothermal sulfidation approach through precise control of metal ratios and sulfidation duration, we significantly enhanced the catalytic performance for the HMF oxidation reaction (HMFOR). Sulfur doping was found to substantially improve the catalyst’s adsorption capability for HMFOR intermediates, thereby enhancing both activity and selectivity toward FDCA production. The optimized FeNi-S@NF nanoflower catalyst demonstrated superior electrocatalytic performance in 1 M KOH + 50 mM HMF, achieving 98.41% conversion rate, 92.08% FDCA yield, and 89.19% Faradaic efficiency at an applied potential of 1.45 V vs a reversible hydrogen electrode (RHE), surpassing most reported transition metal-based electrocatalysts. This research work provides a new strategy for the rapid and low-cost synthesis of two-dimensional sulfide nanoelectrocatalysts for the efficient electrooxidation process of biomass derivatives.
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
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2026
·
IF 4.7
Jia-Yu Gu,Xin-Ran Chen,Fei Li,Wen-Ya Ding,Jia Cao,Wei-Wei Ge,Kai Chen
Three new coordination polymers based on cucurbit[5]uril (Q[5]) have been successfully synthesized. Their chemical formulas are {[Ni(H 2 O) 2 (Q[5])]·(1,5-NDS)}·14H 2 O ( 1 ), {[Co(H 2 O) 2 (Q[5])]·(1,5-NDS)}·15H 2 O ( 2 ), and {[Zn(H 2 O) 2 (Q[5])]·(1,5-NDS)}·10H 2 O ( 3 ). These polymers were obtained through the self-assembly of Q[5] with the corresponding transition metal salts in the presence of 1,5-naphthalenedisulfonic acid (1,5-H 2 NDS). Single- crystal X- ray diffraction analysis of these three coordination polymers has unveiled that the deprotonated 1,5-NDS²⁻ anions play a pivotal role in their self - assembly. Within these complexes, 1,5-NDS²⁻ anions facilitate the direct coordination of Ni 2+ /Co 2+ /Zn 2+ with the carbonyl oxygen atoms at the ports of Q[5], leading to the formation of one - dimensional (1D) supramolecular chains. Moreover, the non - covalent interactions among the 1D chains, along with those arising from the interplay between 1,5 - NDS²⁻ anions and the outer surface of Q[5] within the chains, generate a cooperative effect. This cooperative effect, where the combined action of these interactions is more powerful than the simple sum of their individual contributions, drives the further assembly of the 1D chains into a three-dimensional (3D) supramolecular framework featuring specific channel structures. Performance tests reveal that the Q[5]-Ni 2+ -based supramolecular assembly has high selective recognition for the antibiotic norfloxacin (NFX).
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APPLIED SURFACE SCIENCE
·
2026
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IF 6.9
Yifei Huang,Hongliang Dai,Hongye Bai,Weiqiang Fan
Biomass materials as an abundant renewable resource could replace traditional fossil fuels and non-renewable materials, providing a new solution to achieve sustainable development. In this paper, Ce-modified Co(OH) 2 /Cu 2 O composite catalysts were prepared by an electrodeposition method, and the CoOOH/CuOOH dual active sites were successfully obtained through an electrochemical reconfiguration strategy, and the experimental results showed that the incorporation of Ce induced the formation of high-valent Co and Cu species, which improved the activity of electrocatalytic HMF oxidation. At 0.55 V vs Hg/HgO, the HMF conversion reached 100%, the FDCA yield was 95.04% and the Faraday efficiency was up to 98.47%. These results confirm the effectiveness of Ce-based non-precious metal catalysts in electrochemical reconstruction strategies and demonstrate their potential application in the high-value conversion of biomass derivatives. This finding not only provides a solid research basis for the development of highly efficient catalysts, but is also expected to promote the development of related technological fields.
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WATER SCIENCE AND TECHNOLOGY
·
2016
·Vol. 73
·期 11
·2747-2753
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IF 1.064
Kong Wusong,Qu Hongxia,Chen Peng,Ma Weihua,Xie Huifang
In this study, Cu 2 O-CuO/ZSM-5 nanocomposite was synthesized by the impregnation method, and its catalytic performance for the destruction of AO7 in aqueous solutions was investigated. The morphology, structure and surface element valence state of Cu 2 O-CuO/ZSM-5 were characterized by transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The operating conditions on the degradation of AO7 by Cu 2 O-CuO/ZSM-5, such as initial pH values, concentration of AO7 and catalyst dosage were investigated and optimized. The results showed that the sample had good catalytic activity for destruction of AO7 in the absence of a sacrificial agent (e.g. H 2 O 2 ): it could degrade 91% AO7 in 140 min at 25 °C and was not restricted by the initial pH of the AO7 aqueous solutions. Cu 2 O-CuO/ZSM-5 exhibited stable catalytic activity with little loss after three successive runs. The total organic carbon and chemical oxygen demand removal efficiencies increased rapidly to 69.36% and 67.3% after 120 min of treatment by Cu 2 O-CuO/ZSM-5, respectively.
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