ACS Sensors
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2021
·
IF 7.711
Xiaofang Wang,Tianci Xu,Yue Zhang,Nan Gao,Taotao Feng,Shujun Wang,Meining Zhang
Electrochemical tracking of redox-inactive neurochemicals remain a challenge due to chemical inertness, almost no Faraday electron transfer for these species, and the complex brain atmosphere. In this work, we demonstrate a low-cost, simple-making liquid/liquid interface microsensor (LLIM) to monitor redox-inactive neurochemicals in the rat brain. Taking choline (Ch) as an example, based on the difference in solvation energies of Ch in cerebrospinal fluid (aqueous phase) and 1,2-dichloroethane (1,2-DCE; organic phase), Ch is recognized in the specific ion-transfer potential and distinctive ion-transfer current signals. The LLIM has an excellent response to Ch with good linearity and selectivity, and the detection limit is 0.37 μM. The LLIM can monitor the dynamics of Ch in the cortex of the rat brain by both local microinfusion and intraperitoneal injection of Ch. This work first demonstrates that the LLIM can be successfully applied in the brain and obtain electrochemical signals in such a sophisticated system, allowing one new perspective of sensing at the liquid/liquid interface for nonelectrically active substances in vivo to understand the physiological function of the brain.
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高影响因子
Journal of Biomedical Research
·
2021
·
IF 0
Siwan Wang,Hui Jiang,Jia Wang,Haisi Wu,Ting Wu,Mengnan Ni,Qianqian Zhao,You Ji,Ziting Zhang,Chunming Tang,Huae Xu
As a well-known anticancer drug, paclitaxel (PTX), a first-line chemotherapeutic agent, remains unsatisfactory for gastric cancer therapy. It is reported that triptolide (TPL) could enhance the anti-gastric cancer effect of PTX. Considering the poor solubility of both drugs, we developed a red blood cell membrane-biomimetic nanosystem, an emerging tool in drug delivery, to co-load paclitaxel and triptolide (red blood cell membrane coated PTX and TPL co-loaded poly(lactic- co -glycolic acid) [PLGA] nanoparticles, RP(P/T)). The successful preparation was confirmed in terms of particle size, morphology, and surface markers assays. This biomimetic system could prolong circulation and escape immune surveillance. And these properties were verified by stability, in vitro drug release, and cellular uptake assays. Moreover, the MTT and colony formation assays demonstrated the superior anti-proliferation effect of the RP(P/T) to free drugs. The enhanced antitumor effects of RP(P/T) on migration and invasion were also evaluated by wound-healing and transwell assays. Overall, the bionic co-delivery nanoplatform with improved efficacy in vitro is a promising therapy for gastric cancer.
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PubMed
BMC MICROBIOLOGY
·
2021
·Vol. 21
·期 1
·1-12
·
IF 3.605
Miao Z. H.,Zhou W. X.,Cheng R. Y.,Liang H. J.,Jiang F. L.,Shen X.,Lu J. H.,Li M.,He F.
Background Accumulating evidence have shown that the intestinal microbiota plays an important role in prevention of host obesity and metabolism disorders. Recent studies also demonstrate that early life is the key time for the colonization of intestinal microbes in host. However, there are few studies focusing on possible association between intestinal microbiota in the early life and metabolism in adulthood. Therefore the present study was conducted to examine whether the short term antibiotic and/or probiotic exposure in early life could affect intestinal microbes and their possible long term effects on host metabolism.Results A high-fat diet resulted in glucose and lipid metabolism disorders with higher levels of visceral fat rate, insulin-resistance indices, and leptin. Exposure to ceftriaxone in early life aggravated the negative influences of a high-fat diet on mouse physiology. Orally fed TMC3115 protected mice, especially those who had received treatment throughout the whole study, from damage due to a high-fat diet, such as increases in levels of fasting blood glucose and serum levels of insulin, leptin, and IR indices. Exposure to ceftriaxone during the first 2 weeks of life was linked to dysbiosis of the fecal microbiota with a significant decrease in the species richness and diversity. However, the influence of orally fed ceftriaxone on the fecal microbiota was limited to 12 weeks after the termination of treatment. Of note, at week 12 there were still some differences in the composition of intestinal microbiota between mice provided with high fat diet and antibiotic exposure and those only fed a high fat diet.Conclusion sThese results indicated that exposure to antibiotics, such as ceftriaxone, in early life may aggravate the negative influences of a high-fat diet on the physiology of the host animal. These results also suggest that the crosstalk between the host and their intestinal microbiota in early life may be more important than that in adulthood, even though the same intestinal microbes are present in adulthood.
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JOURNAL OF MEMBRANE SCIENCE
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2019
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IF 7.015
Lingling Zhang,Hui Jia,Jie Wang,Haitao Wen,Juan Li
The electrochemical impedance spectroscopy (EIS) was used to monitor the ion transport of the ion exchange membrane (IEM) interface with ectopic monitoring. But the general monitoring methods were lack of the information in concentration polarization (CP) and fouling formation. In this paper, an in-situ method for monitoring the ion mass transfer by EIS was established which can distinguish the contribution of membrane fouling and CP. The results showed that the in-situ EIS could more comprehensively monitor the desalination process. Membrane fouling, especially the formation of fouling layer on membrane surface, promoted the CP at membrane/solution interface. This was mainly because the ion mass transfer was hindered by serious membrane fouling, so that the ion concentration near the membrane surface was higher than that of the solution, and this phenomenon was gradually aggravated with the increase of membrane fouling. The recovery of membrane performances were assessed by different cleaning methods, it was found that the chemical cleaning effect was superior to inverted electrode according to online EIS. When irreversible fouling was formed in the membrane and then the fouled membrane was chemically cleaned, the formation of the fouling layer was accelerated and the CP was aggravated during membrane reuse.
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BIOORGANIC CHEMISTRY
·
2019
·
IF 3.926
Eryan Guo,Yunwei Hu,Tao Du,Huilin Zhu,Lei Chen,Wei Qu,Jie Zhang,Ning Xie,Wenyuan Liu,Feng Feng,Jian Xu
Alzheimer disease (AD), a prevalent neurodegenerative disorder, is one of the leading causes of dementia. However, there is no effective drug for this disease to date. Picrasma quassioides (D.Don) Benn, a Chinese traditional medicine, was used mainly for the treatment of inflammation, fever, microbial infection and dysentery. In this paper, we reported that the EtOAc extract of Picrasma quassioides stems showed potential neuroprotective activities in l -glutamate-stimulated PC12 and Aβ 25-35 -stimulated SH-SY5Y cell models, as well as improved memory and cognitive abilities in AD mice induced by amyloid-β peptide. Moreover, it was revealed that the anti-AD mechanism was related to suppressing neuroinflammatory and reducing Aβ 1-42 deposition using ELISA assay kits. To clarify the active components of the EtOAc extract of Picrasma quassioides stems, a systematic phytochemistry study led to isolate and identify six β-carboline alkaloids ( 1 – 6 ), seven canthin-6-one alkaloids ( 7 – 13 ), and five quassinoids ( 14 – 18 ). Among them, four β-carbolines ( 1 – 3 , and 6 ) and six canthin-6-ones ( 7 – 11 , and 13 ) exhibited potential neuroprotective activities in vitro . Based on these date, the structure-activity relationships of alkaloids were discussed. Furthermore, molecular docking experiments showed that compounds 2 and 3 have high affinity for both of dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYPKIA) and butyrylcholinesterase (BuChE).
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高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 36
·4580-4585
·
IF 2.378
Xuan Wang,Caicheng Long,Zixin Jiang,Taiping Qing,Kaiwu Zhang,Peng Zhang,Bo Feng
Ascorbic acid (AA) plays an important role in many physiological and biochemical processes. Herein, a novel, rapid and label-free fluorescence strategy was proposed to detect ascorbic acid based on the in situ formation of copper nanoclusters (CuNCs). When reducing ascorbic acid was present, the Cu(II) ions in 3-(N-morpholino) propanesulfonic acid (MOPS) buffer were transformed into CuNCs and showed high fluorescence. The excitation and emission peaks of CuNCs were 340 nm and 580 nm, respectively. The fluorescence intensity was proportional to the concentration of ascorbic acid. Under optimized conditions, the linear range of ascorbic acid detection is 50 to 1000 μM, and the detection limit is 41.94 μM. In addition, this method is successfully applied to the determination of ascorbic acid in real samples (serum samples and vitamin C tablets) and has shown satisfactory results. Besides the simple operation and good detection capability, the signal response of this method to ascorbic acid is fast and can reach maximum saturation in a few minutes. There is no background interference due to the in situ formation of the signal unit (CuNCs). Therefore, the proposed strategy has a strong potential to detect ascorbic acid in medical diagnosis and food safety due to its excellent analytical sensitivity and high selectivity.
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Journal of Materials Chemistry C
·
2019
·Vol. 7
·期 40
·12674-12681
·
IF 6.641
Ming Gong,Jian Yang,Yongsheng Li,Qixin Zhuang,Jinlou Gu
The development of an effective strategy for the accurate detection of cholesterol in human serum is of great significance. Herein, a novel luminescent metal–organic framework (LMOF) probe was successfully constructed for the specific detection of cholesterol in blood. Owing to the large size of the mesopores in NU-1000, carboxymethyl β-cyclodextrin (β-CMCD) was grafted onto the LMOF with a high density and designed as an energy-transfer bridge and an inclusion site for cholesterol recognition. Upon adding a blood sample into the sensing system, the free cholesterol inside the serum competes with Rh6G to form a more stable inclusion complex with β-CMCD, and induces the fluorescence restoration of the ligand in LMOF proportionally, which was quenched in advance by Rh6G due to the fluorescent resonance energy transfer (FRET) effect. The specificity of the employed sensing probe for cholesterol recognition was scarcely affected by other possibly coexisting interfering substances. Furthermore, the LOD value was determined to be as low as 0.4 μM, which is far lower than the normal cholesterol level in human serum. This indicates the practical application of the current developed LMOF sensor for the accurate and rapid detection of cholesterol in disease diagnosis.
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JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
·
2019
·Vol. 24
·期 7
·1077-1085
·
IF 3.632
Wang Xiaoqing,Sun Qian,Zhao Liming,Gong Shuwen,Xu Li
Hydrogen polysulfides (H 2 S n , n > 1), as the oxidized forms of H 2 S, have attracted increasing attention these years due to their involvement in signaling transduction and cytoprotective processes. It is necessary to detect H 2 S n in living systems for the study of their functions. In this work, we report a BODIPY-based near-infrared emitting fluorescence probe NIR-PHS1 , with “turn-on” response, rapid response rate (within 10 min), outstanding selectivity and excellent sensitivity (detection limit = 12 nM) response towards H 2 S n . The probe was successfully applied to the visualizing of endogenous H 2 S n in living cells. Moreover, it can be used for near-infrared in vivo imaging of H 2 S n in living mice. Therefore, NIR-PHS1 could be a potential imaging tool to study the biological roles of H 2 S n in living systems.
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Chinese Herbal Medicines
·
2019
·
IF 0
Ya-yun Liu,Jiao-jiao You,Wei Xu,Ting Zhai,Chun-yuan Du,Yong Chen,Feng-mei Han
Objective The present work was to investigate the protective effects of the aqueous extract of Gynura procumbens (GPAE) against nonalcoholic steatohepatitis (NASH) in mice and NCTC-1469 cells. Methods C57BL/6J mice were fed with methionine and choline-deficient (MCD) diet and administered simultaneously with GPAE (500 and 1000 mg/kg/d, respectively) by gavage for six weeks. The biomarkers of NASH in serum and liver were determined. NCTC-1469 cells were pretreated with 0.25 mmol/L palmitic acid (PA) plus 0.5 mmol/L oleic acid (OA) for 24 h or treated with adenovirus expressing short-hairpin RNA against CFLAR (Ad-shCFLAR) for 24 h and then treated with GPAE (80 and 160 µg/mL, respectively) for 24 h, and the content of cellular biomarkers of NASH was detected. Results In mice treated with MCD , GPAE could decrease the levels of serum ALT , AST, the content of hepatic TG , TC and MDA , repress the activities and protein expression of CYP2E1 and CYP4A and the phosphorylation of JNK, increase the activities of HO-1, CAT and GSH-Px, up-regulate the mRNA expression of PPARα, FABP5, CPT1α, ACOX, SCD-1, mGPAT, MTTP and the protein expression of CFLAR and NRF2. In NCTC-1469 cells treated with PA and OA, GPAE could decrease the content of cellular TG and ROS , promote the uptake of 2-NBDG, up-regulate the protein expression of CFLAR and NRF2. In NCTC-1469 cells treated with Ad-sh CFLAR , GPAE up-regulated the mRNA and protein expression of CFLAR, down-regulated the phosphorylation of JNK, and increased the protein expression of NRF2 and pIRS1 . Conclusion These results indicated that the activation on CFLAR-JNK pathway might be the main anti-NASH mechanism of GPAE, which on the one hand promote the β-oxidation and efflux of fatty acids in liver, and finally reduce hepatic lipid accumulation , on the other hand increase the activities of anti-oxidant enzymes and inhibit the activities of ROS generation enzymes by activating NRF2, and therefore attenuates hepatic oxidative stress damage.
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ACS Catalysis
·
2019
·
IF 12.221
Jiaojiao Zhao,Yurong He,Fei Wang,Wentao Zheng,Chunfang Huo,Xi Liu,Haijun Jiao,Yong Yang,Yongwang Li,Xiaodong Wen
For the hydroformylation of alkenes, developing ligand-free heterogeneous catalysts is a research focus because of both fundamental research interests and potential commercial applications. However, the leaching of active metals (e.g., Co, Rh) during the hydroformylation reaction seriously hinders the development of heterogeneous catalysts. Suppressing metal leaching with the use of an effective protectant is a possible solution to this problem. In this work, Co leaching was suppressed by the addition of suitable protectants (e.g., citric acid, oxalic acid, and formic acid), thus providing a stable heterogeneous catalytic system. In situ Fourier transform infrared proved that toluene and 1-hexene can promote the solvation of Co carbonyls because of the solvent effect. In contrast, formic acid can be converted to formate species (HCOO) on the surface of Co and effectively suppress the formation of Co carbonyls. Theoretical calculations further proved that high CO coverage on the surface of Co leads to the formation of Co carbonyl species. Furthermore, carboxyl radicals resulting from the dissociation of the O–H bonds of organic acids exhibit strong adsorption on the surface of Co. The intrinsic role of the protectants in suppressing metal leaching is attributed to the decreased CO coverage on the surface of Co by competitive adsorption.
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JOURNAL OF APPLIED MICROBIOLOGY
·
2019
·Vol. 127
·期 6
·1716-1726
·
IF 2.683
Cheng R.,Wang L.,Li J.,Fu R.,Wang S.,Zhang J.
AimsTo purify and characterize an exopolysaccharide (EPS) from an Agrobacterium strain ZCC3656 with high EPS‐secreting performance and investigate its anti‐inflammatory activity using lipopolysaccharide (LPS)‐induced macrophage cells in an acute liver injury mouse model.Methods and ResultsTwelve rhizobial strains were compared for EPS fermentation production in modified M9 salts supplemented with mannitol or sucrose as the sole carbon source. Agrobacterium sp. ZCC3656 exhibited the highest EPS yield (21·1 g l−1) and was characterized for EPS production by carbon source utilization, time course fermentation and serial subcultivation assays. The EPS, designated Riclin, was purified by deproteinization using the Sevag method. The combined results of gel permeation chromatography, monosaccharide composition, methylation analysis and nuclear magnetic resonance analyses indicated that Riclin is a succinoglycan‐like polysaccharide comprised of glucose, galactose, succinate and pyruvate at a ratio of 7·8. : 1·0 : 0·9 : 1·1 and has an molecular weight of approximately 2·5 × 106 Da. Riclin inhibited TNF‐α, IL‐1β and IL‐6 expression in LPS‐stimulated RAW 264.7 macrophage cells in a dose‐dependent manner. In addition, Riclin pretreatment increased the survival rate of D‐Gal/LPS treated mice, inhibited serum ALT and AST activities and reduced the production of the inflammatory mediators TNF‐α, IL‐1β and IL‐6.ConclusionsAgrobacterium sp. ZCC3656 is a highly stable EPS‐producing strain. The EPS Riclin from ZCC3656 is a succinoglycan‐type polysaccharide that is noncytotoxic and exhibits remarkable anti‐inflammatory effects in vivo and in vitro.Significance and Impact of the StudySuccinoglycans are well known for good rheological properties and their physiological interactions with plants. However, their potential activity towards mammals has received little attention. Our study revealed that the succinoglycan Riclin exhibited excellent anti‐inflammatory activities and could be considered as a promising reagent in anti‐inflammatory treatment.
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高影响因子
FUEL
·
2019
·
IF 5.128
Ye Tian,Feng Wang,Long-Fei Xie,Yu-Ping Xu,Pei-Gao Duan
Lewis acid-catalyzed in situ transesterification/esterification of tigernut ( Cyperus esculentus ) was conducted in sub/supercritical ethanol. An L 16 (4 4 ) orthogonal design with four factors at four levels was employed to investigate the influence of the process variables on the product distribution and the composition of crude biodiesel (CBD). The four factors were temperature (230–290 °C), time (0–90 min), ethanol to feedstock ratio (E/F) (4/3–16/3 mL/g), and catalyst type (ZnCl 2 , FeCl 3 , SnCl 2 , and H 3 PO 4 ). The results showed that the catalyst type and E/F ratio were the two most influential variables affecting the yield and composition of the CBD. Of the Lewis acids tested, SnCl 2 showed the highest performance in terms of the CBD yield and its esters content. Increasing the E/F ratio essentially led to a higher CBD yield and higher ester content of the CBD. A higher temperature and longer reaction time resulted in an obvious increase in the gas yield but had no significant effect on the yield and quality of the CBD. The produced CBDs had extremely low N and S contents (0.2–0.6 wt% and less than 0.1 wt%, respectively) and were abundant in fatty acid esters (∼70%), mainly ethyl oleate and ethyl palmitate, making it more suitable for subsequent biofuel production.
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