Journal of Environmental Chemical Engineering
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2024
·
IF 7.4
Bo Jing,Xinke Xu,Jingze Wang,Changyan Sun,Wenjun Li,Zhidong Chang
L-lactate is an essential biomarker in clinical diagnostics and food quality assessment. This study introduces a novel ratiometric fluorescence sensor, RhB@Zn-MOF, which was specifically designed for the sensitive and selective detection of L-lactate. Through the strategic incorporation of Rhodamine B (RhB) into Zn-MOF, RhB@Zn-MOF was synthesized, exhibiting dual-emission properties and could effectively distinguish L-lactate in complex biological and food matrices such as milk and sweat based on the competitive absorption mechanism. Notably, the sensor achieves a low detection limit of 0.091 μM and demonstrates excellent stability and reproducibility in varied conditions. Furthermore, the integration of the sensor with smartphone technology enables rapid, real-time analysis, showcasing potential applications in sports medicine, clinical environments, and the food industry.
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高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Yuqing Shi,Jie Liu,Jianwei Xin,Bo Han,Yuqi Zhang,Ji-Jiang Wang
Histidine (His) is an essential amino acid that is closely related to the physiological activities of the human body and human health, being used as a biomarker and therapeutic drug for some diseases. It is desirable to accurately and facilely detect His. Here, an l-histidine (l-His)-sensitive smart two-dimensional photonic hydrogel sensor (CD-2DPCH) was fabricated by modifying NH2-β-cyclodextrin (NH2-β-CD) onto the poly(acrylamide-Co-acrylic acid) hydrogel containing polystyrene two-dimensional photonic crystal array (PS-2DPC). The volume of the CD-2DPCH sensor shrinks upon response to l-His due to the host–guest interaction between NH2-β-CD attached in the hydrogel chains and l-His and the hydrogen bonding interaction between l-His and amides groups on the hydrogel chains. The particle spacing change of ∼95 nm for the PS-2DPC in the hydrogel is achieved, and the chiral recognition of l-His is realized. The particle spacing change of the prepared CD-2DPCH shows a good linear correlation when the l-His concentration changes from 0.1 to 1 and 10 to 400 μM, and the limit of detection is 58 nM. The constructed smart photonic hydrogel sensor was successfully applied to determine l-His in human serum and milk. The fabrication of the sensor is facile, and the detection does not need sophisticated instruments, providing a facile and effective way to identify l-His and a good strategy for sensing other amino acids by modifying different recognition groups.
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高影响因子
PEDOSPHERE
·
2024
·
IF 5.2
Yue CAI,Lirong PU,Lianzhou SHEN,Xinghao WANG,Zhaoyue SUN,Guodong FANG,Dongmei ZHOU,Yunjiang YU,Juan GAO
Silica nanoparticles (nSiO 2 ) have attracted considerable attention in agricultural practices. However, their effects on polycyclic aromatic hydrocarbon (PAH) accumulation in plants remain largely unknown. Thus, this issue was tracked here with a 30-d pot experiment with foliar spraying different sizes of nSiO 2 (100 mg L -1 , 10 mL) on Amaranthus tricolor L. (amaranth). Results showed that 30 nm and 100 nm nSiO 2 increased phenanthrene (PHE) contents in amaranth leaves by 242% ± 88.4% and 158% ± 29.6%, respectively, which was more significant than 2 µm particles (96.0% ± 29.2%). Metabolomics indicated that compared to nSiO 2 , 2 µm SiO 2 more significantly boosted oxidative defense in amaranth leaves, enhanced cell membrane fluidity, and upregulated amino acid metabolism, glycolysis, and gluconeogenesis. In addition, the upregulation of discriminating metabolites was the most significant in 2 µm SiO 2 -treatment, followed by 100 nm and 30 nm. This is mainly because nSiO 2 promoted amaranth growth and decreased average PHE content per unit biomass, namely “dilute effect”. Thus, slighter metabolic disturbances were observed in 30 nm and 100 nm SiO 2 -treated group. The present study revealed the possibility of PAHs accumulation enhancement in vegetables with nSiO 2 foliar spayed. The results of this study provide theoretical guidance regarding to particle size selection for the safe agricultural application of nSiO 2 in PAHs-contaminated areas.
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高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2024
·Vol. 25
·期 2
·1153
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IF 4.9
Hongxue Gao,Zhiqiang Liu,Fengrui Song,Junpeng Xing,Zhong Zheng,Zong Hou,Shu Liu
Cell membrane chromatography (CMC) has been widely recognized as a highly efficient technique for in vitro screening of active compounds. Nevertheless, conventional CMC approaches suffer from a restricted repertoire of cell membrane proteins, making them susceptible to oversaturation. Moreover, the binding mechanism between silica gel and proteins primarily relies on intermolecular hydrogen bonding, which is inherently unstable and somewhat hampers the advancement of CMC. Consequently, this investigation aimed to establish a novel CMC column that could augment protein loading, enhance detection throughput, and bolster binding affinity through the introduction of covalent bonding with proteins. This study utilizes polydopamine (PDA)-coated silica gel, which is formed through the self-polymerization of dopamine (DA), as the carrier for the CMC column filler. The objective is to construct the HK-2/SiO2-PDA/CMC model to screen potential therapeutic drugs for gout. To compare the quantity and characteristics of Human Kidney-2 (HK-2) cell membrane proteins immobilized on SiO2-PDA and silica gel, the proteins were immobilized on both surfaces. The results indicate that SiO2-PDA has a notably greater affinity for membrane proteins compared to silica gel, resulting in a significant improvement in detection efficiency. Furthermore, a screening method utilizing HK-2/SiO2-PDA/CMC was utilized to identify seven potential anti-gout compounds derived fromPlantago asiaticaL. (PAL). The effectiveness of these compounds was further validated using an in vitro cell model of uric acid (UA) reabsorption. In conclusion, this study successfully developed and implemented a novel CMC filler, which has practical implications in the field.
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高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Zhiqiang Wang,Mingfan Cui,Hao Wang,Lei Ma,Yehong Han,Dandan Han,Hongyuan Yan
The defatted seeds of evening primrose (DE), a by-product of evening primrose oil extraction, are currently underutilized. This study aimed to valorize DE by examining its effects on melanogenesis and tyrosinase activity in zebrafish embryos and in vitro , and an innovative affinity-labeled molecular networking workflow was proposed for the rapid identification of tyrosinase inhibitors in DE. Our results indicated DE significantly reduced melanin content (53.3 % at 100 μg/mL) and tyrosinse activity (80.05 % for monophenolase and 70.40 % for diphenolase at 100 μg/mL). Furthermore, through the affinity-labeled molecular networking approach, 20 compounds were identified as potential tyrosinase inhibitors within DE, predominantly flavonoids and tannins characterized by catechin and galloyl substructures. Seven of these compounds were isolated and their inhibitory effects on tyrosinase were validated using functional assays. This study not only underscores the potential of DE as a rich source of natural tyrosinase inhibitors but also establishes the effectiveness of affinity-labeled molecular networking in pinpointing bioactive compounds in complex biological matrices.
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高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 7
·4298-4311
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IF 3.2
Jing Tan,Hongwei Cao,Xiaoxue Wang,Sen Li,Hongdong Song,Kai Huang,Yu Zhang,Jun Lu,Xiao Guan
This study explored the mechanism of l -lysine intervention in wheat gluten protein (WG) gel formation under a microwave (MW) field. The results showed that the MW treatment had higher ζ -potential values at the same heating rate. After adding l -lysine, the solution conductivity and dielectric loss were significantly increased. Moreover, the WG gel strength enhanced 4.40% under the MW treatment. The Fourier spectra showed that the α-helix content was decreased 13.78% with the addition of lysine. The ultraviolet absorption spectra and fluorescence spectra indicated that MW irradiation impacted the interactions between WG molecules more effectively than the water bath heating, promoting the denaturation and unfolding of the protein structure. In addition, scanning electron microscopy analysis showed that the incorporation of lysine promoted an ordered network structure formation of the protein, which enhanced the gel properties. This indicated that the zwitterion of l -lysine played a regulatory role in the aggregation of proteins in the MW field.
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高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Yiyang Huang,Yichang Xu,Ziyan Huang,Jiannan Mao,Yujian Hui,Min Rui,Xinzhao Jiang,Jie Wu,Zhouye Ding,Yu Feng,Yong Gu,Liang Chen
The bone immune microenvironment can influence the occurrence and progression of bone defects. To date, research on promoting macrophage M2 polarization to improve bone injury repair has been insufficient. In this study, we designed an injectable poly(L-lactic acid) (PLLA) porous microsphere that forms calcium phosphate crystals on its surface by binding to melatonin, followed by bionanomimetic mineralization in vitro. The microsphere is injectable and degradable, and its release of melatonin (MT) and calcium phosphate (CaP) crystals promotes macrophage M2 polarization, reprogramming of macrophages, and enhanced osteogenesis. After LPS stimulation, the proportion of M2-polarized macrophages in the MS@CaP@MT group was 39.2 ± 2.7%, significantly higher than that in other groups (P < 0.05). Further, in the MS@CaP@MT group, rats exhibited bone mineral densities of 129.4 ± 12.8 mg cc−1 at 2 weeks and 171.6 ± 13.6 mg cc−1 at 4 weeks in the defect area, which were significantly higher than those in other groups (P < 0.05). Using an animal model of femoral condylar defects, we demonstrated that MT PLLA porous microspheres loaded with calcium phosphate crystals can improve the immune microenvironment and form a microsphere-centered osteogenesis model. This significantly accelerates bone defect repair and provides a potential strategy for bone defect treatment.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Ming Wei,Zhichao Ma,Zhuli Zhang,Ke Chi,Shulin Zhang,Wenting Tong,Wenbo Lu
Tert-butylhydroquinone (TBHQ) is a widely used synthetic phenolic antioxidant found in edible oils and other fried foods. Nevertheless, the excess use of TBHQ can reduce food quality and impact public health. In this paper, we reported the synthesis of a nanocomposite consisting of carbon and nitrogen co-doped nickel oxide (NiO-N/C-700), which was used to modify a pencil graphite electrode for the sensitive detection of TBHQ. The carbon source was biochar derived from invasive species Solidago canadensis L., combined with inexpensive pencil graphite, which reduced overall cost. The as-prepared TBHQ electrochemical sensor exhibited a linear range of 0.02–200 μM, a limit of detection of 5.53 nM. In particular, this sensor can be applied for the quantitative detection of TBHQ in edible oil, fried food and crisps, achieving a satisfactory recovery rate of 99.30 %–106.40 %, promoting TBHQ detection in actual food samples.
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ACS Nano
·
2025
·
IF 16
Zhipeng Guo,Tingting Zhang,Zhao Chen,Junpeng Niu,Md Rasel,Enze Luo,Yuetong Li,Nan Xu,Mahmood Ul Hassan,Hafiz Abdul Kareem,Quanzhen Wang,Xuebing Yan,Yuxia Guo
“Nanophytovirology” is a potential management approach to combat plant viral diseases. Herein, the impact of foliar application of CeO2 nanoparticles (CeO2-NPs) on the growth, plant cell ultrastructure, and physiology of alfalfa mosaic virus (AMV)-infected alfalfa seedlings was explored at different concentrations and application strategies. In a solar greenhouse, 20 mL of CeO2-NP suspensions of 50, 100, 200, and 500 mg/L was sprayed onto the plant surface every other day for 9 days. As a result, the total volume applied per pot was 100 mL. It showed that the beneficial effects of CeO2-NPs on infected alfalfa seedlings were dependent on the concentration and application period. Compared to the infected control, foliar application with 100 mg/L CeO2-NPs before AMV infection (CeO2-NPs-BVI) showed the greatest virus suppression efficacy; significantly reduced the disease indices by 58.87%; improved growth, yield, and nutritional quality; and significantly increased plant height, plant dry biomass, crude protein, and crude fat contents and relative feeding value by 15.17, 37.12, 18.77, 71.19, and 10.09%, respectively. Moreover, CeO2-NPs-BVI maintained the chloroplast quantity and structure of leaf cells and significantly enhanced the chlorophyll content and photosynthesis rate of alfalfa leaves by 36.14 and 40.13%, respectively, as compared to infected control. Mechanistically, cell ultrastructure, physiology, and transcriptomic analyses revealed that (1) CeO2-NPs effectively mitigated AMV-induced chloroplast structure damage, subsequently enhancing photosynthesis and carbon fixation in photosynthetic organisms, providing sufficient energy and antiviral activities for maintaining plant growth and development; (2) CeO2-NPs reduced the AMV’s ability to bind to host receptors and evade host immune recognition, significantly activating and boosting plant systemic immunity by down-regulating ABA and ETH levels and upregulating SA, IAA, and BR levels; (3) CeO2-NPs activated the plant antioxidative systems to eliminate excess reactive oxygen species. These findings provide significant insight into the potential of CeO2-NPs as highly efficient antiviral agent.
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高影响因子
Environmental Science-Processes & Impacts
·
2024
·Vol. 26
·期 7
·1171-1183
·
IF 4.3
Wenxuan Wang,Haiou Wang,Xiaoyu Ren,Wenxiao Zhang,Qian Li
To explore key factors involved in the uptake, translocation and accumulation of organophosphate esters (OPEs), computer simulation analysis and hydroponic experiments were executed. Lipid transporters with stocky-like active (SAC) cavities usually showed stronger binding affinities with the OPEs, especially when the SAC cavities belong to the Fish Trap model according to molecular docking. In our hydroponic trial, the binding affinity and gene expression of the lipid transporters and log Kow of the OPEs could be charged to the uptake, translocation and accumulation of the OPEs; however, these three factors played various important roles in roots and shoots. In detail, the effect of gene expression and binding affinity were stronger than log Kow in roots uptake and accumulation, but the result was the opposite in the shoots translocation. Transporters OsTIL and OsLTPL1 among all investigated transporters could play key roles in transporter-mediated OPE uptake, translocation and accumulation in the roots and shoots. OsMLP could be involved in the bidirected vertical translocation of the OPEs. OsLTP2 and OsLTP4 mainly acted as transporters of the OPEs in roots.
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BIOSENSORS & BIOELECTRONICS
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2018
·
IF 8.173
Fang-Kai Du,Hui Zhang,Xue-Cai Tan,Jun Yan,Min Liu,Xiao Chen,Ye-Yu Wu,De-Fen Feng,Quan-You Chen,Jian-Mei Cen,Shao-Gang Liu,Yu-Qin Qiu,He-You Han
In this work, the feasibility of a novel sensitive electrochemiluminescence aptasensor for the detection of lysozyme using Ru(bpy) 3 2+ [email protected] L -lysine-Au ( [email protected] ) nanocomposites labeling as an indicator was demonstrated. The substrate electrode of the aptasensor was prepared by depositing gold nanoparticles (AuNPs) on 3D graphene-modified electrode. The lysozyme binding aptamer (LBA) was attached to the 3D graphene/AuNPs electrode through gold-thiol affinity, hybridized with a complementary single-strand DNA (CDNA) of the lysozyme aptamer labeled by [email protected] as an electrochemiluminescence intensity amplifier. Thanks to the synergistic amplification of the 3D graphene, the AuNPs and [email protected] NPs linked to Ru(bpy) 3 2+ -ECL further enhanced the ECL intensity of the aptasensor. In presence of lysozyme, the CDNA segment of the self-assembled duplex was displaced by the lysozyme, resulting in decreased electrochemiluminescence signal. Under the optimized conditions, the decrease in electrochemiluminescence intensity varied proportionally with the logarithmic concentration of the lysozyme from 2.25 × 10 –12 to 5.0 × 10 −8 mol L −1 , and the detection limit was estimated to 7.5 × 10 –13 mol L −1 . The aptasensor was further tested in real samples and found reliable for the detection of lysozyme, thus holding great potential application in food safety researches and bioassay analysis.
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高影响因子
Materials Chemistry Frontiers
·
2018
·Vol. 2
·期 4
·768-774
·
IF 0
Na Song,Yun Zhu,Fuqiu Ma,Ce Wang,Xiaofeng Lu
A facile and sensitive detection of L-cysteine is essential for biosensors, environmental monitoring and medical diagnosis. In this study, a simple approach has been demonstrated to prepare Prussian blue (PB)/polypyrrole (PPy) hybrid nanofibers as efficient peroxidase-like mimics for the determination of L-cysteine. α-Fe2O3 nanofibers act as both templates and a source of Fe3+ to polymerize pyrrole in an acidic environment, resulting in the formation of PB/PPy hybrid nanofibers. The as-prepared PB/PPy hybrid nanofibers show a superior catalytic activity as peroxidase mimics over α-Fe2O3 nanofibers. Due to the high catalytic efficiency of the resultant PB/PPy hybrid nanofibers, we have developed a simple colorimetric approach for the rapid and sensitive determination of L-cysteine with a detection limit of about 16 nM over a wide linear range from 1 to 20 μM. In addition, the detection system displays a high selectivity toward L-cysteine over other amino acids, glucose, and common ions. This work provides a new strategy for the preparation of peroxidase-like nanomaterials with improved catalytic activity, which shows great promise for application in highly sensitive detection.
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危险品化学品经营许可证（带存储）