Dermatologica Sinica
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2025
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IF 2.2
Peng Yan,He Ling,Yang Xiaoqiong,Zhao Aijie
Background: Androgenetic alopecia (AGA), a kind of nonscarring baldness, is an androgen-dependent autosomal dominant polymorphism. Compound glycyrrhizin (CG), a compound preparation-containing glycyrrhizin, aminoacetic, and methionine, has been used to treat alopecia areata. Objectives: Our study aimed to explore the role of CG in hair regrowth in testosterone-induced AGA mouse model and the proliferation of human hair dermal papilla cells (HDPCs) and the related molecular mechanisms. Methods: The AGA mouse model was established through the injection of testosterone. The in vivo therapeutic effects of CG on AGA were assessed by detecting skin color changes, hair growth time, average hair length, and histological score. Human HDPCs after CG treatment were subjected to CCK-8 assay to evaluate cell proliferation. The impacts of CG on growth factor expression and the Wnt/β-catenin signaling in mouse dorsal skins and HDPCs were determined by undertaking western blotting, reverse transcription-quantitative polymerase chain reaction, and immunofluorescence staining. The Wnt/β-catenin pathway inhibitor XAV939 was used to validate the beneficial role of Wnt/β-catenin pathway activation in CG-mediated hair growth. Results: Our results showed that CG facilitated new hair growth and increased hair follicle numbers on the depilated dorsal area of the model mice. Furthermore, CG administration upregulated p-GSK-3β, β-catenin, cyclin D1, Axin2, LEF1, fibronectin, and survivin levels in the dorsal skins of model mice. In vitro assays revealed that CG promoted cell proliferation, elevated the mRNA levels of growth factors-related genes (insulin-like growth factors-1 (IGF-1), fibroblast growth factor-2 (FGF-2), and hepatocyte growth factor (HGF)), and increased p-GSK-3β, β-catenin, cyclin D1, Axin2, LEF1, fibronectin, and survivin protein levels in HDPCs. Importantly, XAV939 treatment debilitated the promotion of CG on hair regrowth in model mice and human hair dermal papilla cell (HPDC) proliferation. Conclusion: The beneficial role of CG in hair regrowth in AGA mice and HDPC cell proliferation depends on the Wnt/β-catenin pathway activation and the subsequent growth factor production in hair follicles. These results imply the potential of CG in the treatment of AGA.
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BIOELECTROCHEMISTRY
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2025
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IF 4.5
Huawu Mao,Lidong Ding
Early and accessible diagnosis of Alzheimer's disease (AD) is crucial for effective intervention, yet current methods like amyloid-PET(positron emission tomography) imaging remain cost-prohibitive and lack scalability. Here, we present a miniaturized electrochemical platform for ultrasensitive detection of amyloid-β oligomers (AβOs), pathogenic biomarkers implicated in early AD progression. The system employs a disposable screen-printed electrode functionalized with gold nanoparticles (AuNPs) and thiolated aptamers, leveraging Au S bonds for oriented aptamer immobilization and AβO-specific recognition. This aptasensor achieves a linear detection range of 10 pg/mL to 100 μg/mL (R 2 = 0.995) with a limit of detection (LOD) of 2.3 pg/mL (S/N = 3) in human serum, coupled with robust recovery rates (94.9–108.89 %) and low inter-assay variability (RSD < 5.5 %). Integrated with a handheld analyzer (90 × 50 × 8 mm, 30 g) executing differential pulse voltammetry (DPV), the platform wirelessly transmits data to a smartphone app, delivering quantitative results within 20 s at <$1 per test. Preclinical validation using blood from mice injected with AβO protein showed that the platform's test results were highly correlated with the injected dose (R 2 = 0.995, p < 0.001) and outperformed ELISA in terms of efficiency and cost. By combining laboratory-grade performance, portability, and scalability, the system addresses unmet needs in community-based AD screening and has scalable potential for monitoring neurodegenerative proteomics.
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高影响因子
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
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2025
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IF 3.3
Hong-Yu Li,Wei-Lan Zeng,Yi-Wen Ye,Xin Chen,Ming-Ming Zhang,Yi-Si Chen,Cui-Ting Liu,Zhun-Qiang Zhong,Jing Li,Yan Wang
Glia maturation factor-β (Gmfb), an actin filament debrancher, was initially identified in brain and recently linked to liver diseases. To investigate the role of hepatocyte Gmfb (hep-Gmfb) in liver reparative regeneration, hepatocyte-specific gmfb knockout (HepGKO) and overexpression (HepGOE) zebrafish strains were constructed. Both transgenic and wild-type (WT) zebrafish underwent partial hepatectomy (PHX) or were fed high fat, high cholesterol diets to model metabolism-associated steatotic liver disease (MASLD). Under physiological conditions, the HepGKO, HepGOE, and WT fish displayed similar survival, gross appearance, and liver histology. Following PHX, WT liver gmfb levels positively correlated with cell proliferation and proinflammatory cytokine levels. HepGOE showed enhanced regeneration and reduced liver steatosis compared to WT, while HepGKO exhibited opposite effects. In MASLD, WT liver gmfb increased with disease progression. HepGKO experienced worsening liver enlargement, steatosis, ballooning, inflammation, and endoplasmic reticulum stress, while HepGOE showed improvements. HepGOE liver had the highest cell proliferation, but all three groups showed similar levels of cell apoptosis. Moreover, elevated proinflammatory cytokines were observed across MASLD groups, being the highest in HepGKO and lowest in HepGOE. However, stat3 activation was the lowest in HepGKO and highest in HepGOE, while jnk and mapk/erk activation was consistent across the MASLD groups. In il6-treated primary hepatocytes, gmfb abundance influenced stat3 activation, and hep-gmfb abundance significantly affected actin filaments distribution in hepatocytes both in vivo and vitro. Conclusions: Hep-Gmfb boosts regenerative processes by enhancing hepatocyte proliferation, alleviating fatty liver histological abnormalities, and modulating the Il6/Stat3 signaling, potentially through remodeling of actin-filament network within hepatocytes.
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FOOD CHEMISTRY
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2025
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IF 9.8
Haoling Yang,Bing Wang,Yanjun Wei,Chenchen Gu,Xianggen Wu
Novel mung bean protein (MBP)-glycyrrhizin acid (GA) complexes were fabricated via non-covalent interactions, optimized for particle size, zeta potential, solubility, and emulsifying properties. GA addition order (pre/post pH-ultrasonic treatment) influenced complex characteristics. MBP-GA effectively stabilized high internal-phase Pickering emulsions (HIPPEs), forming dense interfacial networks that enhanced physical stability. Compared to MBP alone, MBP-GA improved β-carotene encapsulation efficiency by 97.99 % and significantly enhanced its thermal/photo-stability in HIPPEs, with GA addition order affecting stability outcomes. In vitro digestion revealed MBP-GA increased β-carotene bioaccessibility by 39.88 %. In vivo, MBP-GA-stabilized HIPPEs boosted total antioxidant capacity in mouse eyeballs versus MBP systems. This study demonstrates MBP-GA as a multifunctional emulsifier for HIPPEs, enabling efficient β-carotene delivery with enhanced stability and bioactivity, highlighting its potential in functional food applications.
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ACS Applied Nano Materials
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2025
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IF 5.5
Jiangyan Lu,Xueyan Wu,Zhuoran Yi,Kun Xiang,Sakil Mahmud,Zong Lu,Dahao Lian,Weiting Wang,Yimeng Feng,Zhu Wang,Xuexia Huang,Zhu Xiong
To address the challenge of efficient antimony [Sb(III)] removal from wastewater, we developed a hierarchically structured β-FeOOH@PDA/PEI-PTFE composite membrane that combines synergistic adsorption and photocatalytic functions. A conformal PDA/PEI layer was deposited on PTFE membranes to promote Fe3+ chelation and support the in situ growth of vertically aligned β-FeOOH nanorods. Multiscale characterization (SEM, EDS, ATR-FTIR, XRD, and XPS) confirmed uniform β-FeOOH distribution, covalent Fe–O–C bonding, and the presence of functional groups (−NH2, Fe–OH), which are critical for Sb(III) uptake. The membrane demonstrated excellent adsorption performance, achieving 95% removal at pH 7, with a capacity of 420.01 mg/m2 and 5 mg/L Sb(III). Adsorption kinetics and isotherms followed pseudo-second-order and Freundlich models, indicating heterogeneous multilayer chemisorption. The presence of competitive ions (e.g., HPO42–, CO32–) had minimal impact, highlighting the membrane’s selectivity. Under UV irradiation, photocatalytic oxidation further increased Sb(III) removal to 98% within 60 min through •OH/h+-mediated oxidation to Sb(V), supported by Fe3+/Fe2+ redox cycling, as confirmed by XPS and ESR analyses. The PDA/PEI matrix enhanced structural stability via π–π conjugation and hydrogen bonding, allowing the membrane to retain 76.3% of its efficiency after five regeneration cycles, despite minor photocorrosion and pore blockage. This study reveals a dual-functional mechanism involving electrostatic attraction, complexation, and photocatalysis, offering a sustainable and scalable strategy for antimony remediation in complex water environments.
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Molecular Catalysis
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2025
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IF 4.9
Hui Xia,Yameng Zhou,Yuanyuan Zheng,Qing Xu,Feng Xue
There is increasing interest in the protein engineering of β-glucosidases for improving transformation of glycosides. In this study, a novel steric hindrance scanning strategy is applied to β-glucosidase BgMd, resulting in six single-point mutants with significantly increased activity. Further iteration results in the optimized mutant E329K/R72N/A94H/T135H showed a remarkable 26.1-fold improvement in activity over the wild type. The time needed for the hydrolysis of 20 g/L polydatin is cut in half, and the conversion rate is increased to 95.93 %. Mutant A94T/V336T hydrolyzes 2 g/L of ginsenoside Rba1 in 48 h to generate CK with a yield increase from 26.94 to 76.38 %. The mutant E329N hydrolyses 2 g/L of astragaloside in 48 h with a yield increase from 30.19 to 96.23 %. BgMd and mutants exhibit optimal activity at 50–60 °C and pH 7.0, with enhanced stability and improved tolerance in select mutants. Molecular docking and molecular dynamics showed that the substrate pocket is split in two, and the catalytic effect of the mutants is largely explained. Molecular dynamics simulations provided evidence of structural changes and reduced interaction energies in the mutants. These results validate the steric hindrance strategy as an effective method to apply β-glucosidase in the biotransformation of glycosides.
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PLANT CELL AND ENVIRONMENT
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2025
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IF 6.3
Lanxin Luo,Ting Gao,Yanni Deng,Mengyao Chai,Bo Li,Hao Ni,Kai Wang,Mengting Zhang,Yaojia Liu,Hao Jiang,Chuankui Song,Tingting Jing
Herbivore-induced plant volatiles play a crucial role in enhancing plant resistance and adaptability. However, the key volatiles triggering this response and their activation mechanisms remain largely unknown. Although tea aphids ( Toxoptera aurantii ) are common tea plantation pests, the underlying biochemical pathways by which aphid-induced volatiles in Camellia sinensis contribute to aphid resistance are not well understood. In this study, we measured jasmonic acid (JA) accumulation induced by aphid feeding using LC-MS and DESI-IMS analysis. We also identified a diverse array of aphid-induced volatiles through GC-MS. Notably, tea aphids stimulated β-Elemene accumulation, enhancing ‘Shuchazao’ resistance to tea aphids. We also evaluated the insecticidal activity of β-Elemene through contact toxicity and repellent bioassays. For the first time, we identified aphid-induced CsELE as a terpene synthase enzyme catalyzing the final step in β-Elemene biosynthesis from farnesyl pyrophosphate. Transient overexpression and gene silencing of CsELE affected β-Elemene accumulation, thereby influencing tea plant resistance to aphids. Furthermore, Dual-Luciferase and Y1H assays revealed that MYC2a, a core transcription factor in the JA signaling pathway, positively regulates CsELE expression. Overall, this study advances our understanding of the transcriptional regulation of tea volatiles in response to pest attacks, providing new insights into the ecological significance of terpene volatiles under biotic stress.
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MOLECULAR NUTRITION & FOOD RESEARCH
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2025
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IF 4.2
Qianru Xiang,Xiao Chen,Enhui Tang,Zhi Chen,Zijun Xia,Wenzhen Liao
Current research indicates that insulin secretion deficiency in β-cells contributes to Type 2 diabetes mellitus (T2DM), which is associated with neuropeptide Y 1 receptor (Npy1r) overexpression from neuropeptide Y (NPY) system dysregulation. To date, limited literature has explored nobiletin (NOB) as a circadian modulator for restoring β-cell function through Npy1r regulation. This study investigates NOB's stimulatory effects on insulin secretion via Npy1r and clock-modulatory signaling to elucidate its underlying mechanism. The findings demonstrated that NOB ameliorated hyperglycemia and promoted insulin secretion in two distinct mouse models: T2DM induced by a high-fat diet with low-dose streptozotocin, and acute hyperglycemia triggered by NPY. Moreover, NOB reduced NPY family peptides in the serum and suppressed Npy1r overexpression in β-cells. Comprehensive investigations revealed that NOB mitigated Npy1r-mediated β-cell dysfunction through activating clock-modulatory signaling, evidenced by restored Bmal1::Luc bioluminescence rhythmicity in mouse insulinoma6 (MIN6) cells. Molecular docking confirmed a direct NOB-Npy1r interaction, while mechanistic analyses demonstrated modulation of the retinoic acid-related orphan receptors (RORs)/Bmal1-Yes-associated protein (Yap) pathway via Yap suppression and Bmal1 activation, based on the interventions with palmitic acid, BMS 193885 (Npy1r antagonist), and NPY. Therefore, this study provides evidence that NOB can protect β-cells from insulin secretion dysfunction by downregulating Npy1r expression and activating the RORs/Bmal1-Yap pathway.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
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IF 8.5
Chuying Huo,Xueting Zhang,Quanwei Zhang,Jing Li,Wenting Zhao,Simin Qi,Hauyee Fung,Wei Yang,Xi Shi,Huiyuan Cheng,Hubiao Chen,Joshua Kashun Ko,Karl Wahkeung Tsim,Hiuyee Kwan,Lifeng Li,Quanbin Han
While Dendrobii β-1,4-mannan (DOP) shows promise in treating colitis, previous research only correlated changes in gut microbiota composition with DOP's effects, without elucidating the specific mechanism by which gut microbiota mediates DOP's bioactivities. This study addresses this crucial gap by demonstrating the essential role of vancomycin-sensitive gut bacteria in mediating the anti-colitis effects of DOP. Using targeted antibiotic perturbation, we found that vancomycin, but not neomycin, abolished both DOP degradation and its therapeutic effects in colitis mice. This loss of efficacy was attributed to the depletion of mannan endo-1,4-β-mannosidase (EC 3.2.1.78), a verified enzyme for DOP catabolism. The expression of this enzyme was increased in DOP-treated microbiota, together with a stronger catalytic ability in DOP degradation. We further identified Bifidobacterium pseudolongum , a vancomycin-sensitive species, as an important contributor to DOP fermentation and its anti-colitis effects. This bacterium initiates DOP's degradation by cleaving it into bioactive mannan oligosaccharides. In summary, this research reveals that the breakdown of DOP by mannan endo-1,4-β-mannosidase in vancomycin-sensitive B. pseudolongum plays a significant role in its anti-colitis effects. This work advances the field by demonstrating that vancomycin-sensitive bacteria, such as B. pseudolongum , mediate DOP's efficacy and establishing polysaccharide catabolism as critical for its therapeutic activity.
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EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
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2025
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IF 1.8
Pan Gao,Xinlian Zhao,Xiaoming Jiang,Yuling Zheng,Xinghe Zhang,Wu Zhong,Xingguo Wang
Iron walnut oil is recognized for its high content of polyunsaturated fatty acids and bioactive compounds, which face significant oxidative stability challenges during storage and processing. This study investigates the antioxidant interactions among γ-tocopherol, ellagic acid, and β-sitosterol in iron walnut oil during the thermal oxidation, aiming to enhance its oxidative stability. The antioxidant efficacy of γ-tocopherol, ellagic acid, and β-sitosterol, both individually and in combination, was evaluated. γ-Tocopherol demonstrated the strongest antioxidant activity, and its combination with ellagic acid exhibited a synergistic effect (SE), markedly enhancing the oxidative stability of iron walnut oil by reducing hydrogen peroxide formation and preserving unsaturated fatty acids, whereas β-sitosterol exhibited limited efficacy, suggesting antagonistic interactions. The effects on fatty acid profile and polar components were analyzed via nuclear magnetic resonance (NMR) and total polar content analysis. The findings suggest that optimizing natural antioxidant combinations can effectively extend the shelf life of iron walnut oil and improve its application potential in the food industry, aligning with the demand for clean-label and sustainable products. Practical Application : The study provides valuable insights for food industry professionals working with polyunsaturated fatty acid (PUFA)-rich oils. By demonstrating that γ-tocopherol and ellagic acid significantly enhance the oxidative stability of iron walnut oil, the research offers practical guidance for formulating walnut oil-based products with extended shelf life. These findings can directly inform the development of natural antioxidant systems in food preservation, aligning with consumer demands for clean-label and sustainable products. Researchers can use this information to optimize antioxidant combinations in various food formulations, potentially reducing waste and improving the nutritional profile of food products. The study also highlights the importance of careful antioxidant selection to avoid antagonistic interactions, which is crucial for the industrial application of health-focused products.
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高影响因子
eLife
·
2018
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IF 7.616
Dao-Lai Zhang,Yu-Jing Sun,Ming-Liang Ma,Yi-jing Wang,Hui Lin,Rui-Rui Li,Zong-Lai Liang,Yuan Gao,Zhao Yang,Dong-Fang He,Amy Lin,Hui Mo,Yu-Jing Lu,Meng-Jing Li,Wei Kong,Ka Young Chung,Fan Yi,Jian-Yuan Li,Ying-Ying Qin,Jingxin Li,Alex R B Thomsen,Alem W Kahsai,Zi-Jiang Chen,Zhi-Gang Xu,Mingyao Liu,Dali Li,Xiao Yu,Jin-Peng Sun
Luminal fluid reabsorption plays a fundamental role in male fertility. We demonstrated that the ubiquitous GPCR signaling proteins Gq and β-arrestin-1 are essential for fluid reabsorption because they mediate coupling between an orphan receptor ADGRG2 (GPR64) and the ion channel CFTR. A reduction in protein level or deficiency of ADGRG2, Gq or β-arrestin-1 in a mouse model led to an imbalance in pH homeostasis in the efferent ductules due to decreased constitutive CFTR currents. Efferent ductule dysfunction was rescued by the specific activation of another GPCR, AGTR2. Further mechanistic analysis revealed that β-arrestin-1 acts as a scaffold for ADGRG2/CFTR complex formation in apical membranes, whereas specific residues of ADGRG2 confer coupling specificity for different G protein subtypes, this specificity is critical for male fertility. Therefore, manipulation of the signaling components of the ADGRG2-Gq/β-arrestin-1/CFTR complex by small molecules may be an effective therapeutic strategy for male infertility.
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PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2024
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IF 4.2
Chaojin Si,Hongjun Yang,Xiaoyan Wang,Qiaoxin Wang,Min Feng,Huayue Li,Yuqi Feng,Jiayuan Zhao,Ying Liao
Beta-cypermethrin (β-CYP) consists of four chiral isomers, acting as an environmental estrogen and causing reproductive toxicity, neurotoxicity, and dysfunctions in multiple organ systems. This study investigated the toxic effects of β-CYP, its isomers, metabolite 3-phenoxybenzoic acid (3-PBA), and 17β-estradiol (E2) on HTR-8/SVneo cells. We focused on the toxic mechanisms of β-CYP and its specific isomers. Our results showed that β-CYP and its isomers inhibit HTR-8/SVneo cell proliferation similarly to E2, with 100 μM 1S-trans-αR displaying significant toxicity after 48 h. Notably, 1S-trans-αR, 1R-trans-αS, and β-CYP were more potent in inducing apoptosis and cell cycle arrest than 1R-cis-αS and 1S-cis-αR at 48 h. AO/EB staining and flow cytometry indicated dose-dependent apoptosis in HTR-8/SVneo cells, particularly at 100 μM 1R-trans-αS. Scratch assays revealed that β-CYP and its isomers variably reduced cell migration. Receptor inhibition assays demonstrated that post-ICI 182780 treatment, which inhibits estrogen receptor α (ERα) or estrogen receptor β (ERβ), β-CYP, its isomers, and E2 reduced HTR-8/SVneo cell viability, whereas milrinone, a phosphodiesterase 3 A (PDE3A) inhibitor, increased viability. Molecular docking studies indicated a higher affinity of β-CYP, its isomers, and E2 for PDE3A than for ERα or ERβ. Consequently, β-CYP, its isomers, and E2 consistently led to decreased cell viability. Transcriptomics and RT-qPCR analyses showed differential expression in treated cells: up-regulation of Il24 and Ptgs2 , and down-regulation of Myo7a and Pdgfrb , suggesting the PI3K-AKT signaling pathway as a potential route for toxicity. This study aims to provide a comprehensive evaluation of the cytotoxicity of chiral pesticides and their mechanisms.
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高影响因子
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