JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Zonghan Wang,Fawen Yin,Deyang Li,Xinmiao Wang,Chao Guo,Donghong Liu,Beiwei Zhu,Dayong Zhou
Catechins are widely recognized for superb antioxidant capability, but their application as food antioxidants is hindered by susceptibility to auto-browning under high-moisture conditions. Here, we proposed a strategy of ordered encapsulation with cyclodextrin-based metal–organic frameworks (CD-MOFs) to alleviate the auto-browning phenomenon of catechins while preserving their antioxidant capability and demonstrated the feasibility of this strategy via selecting epigallocatechin-3-gallate (EGCG) as a model. Even in aqueous solutions, EGCG@CD-MOFs still possessed delayed browning, in contrast with pristine EGCG, characterized by suppressed efficiencies on the generation of oxidative dimers (theasinensin A) and semiquinone radicals. Mechanism insights revealed that ordered encapsulation brought dual regulations on the self-aggregation behavior of EGCG: EGCG@CD-MOFs exhibited a gradual structural collapse from the framework toward irregular aggregates as O–K bonds broke progressively, which restricted molecular mobility of EGCG, and EGCG molecular conformations became constrained by the structure of EGCG@CD-MOFs as well as rich intermolecular forces, even after structural collapse.
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高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2023
·
IF 3
Sun Ziyi,Zhang Gaoning,Li Xiaoru,Cui Qingqi,Gong Lili,Tian Zhenhua
This study investigated the chemical and volatile characteristics of sea buckthorn fruits from three different regions in China. The chemical composition of the volatile oil was determined by using a non-targeted gas chromatography and mass spectrometry (GC/MS) method and the differences in chemical composition among the three producing areas were compared by heatmap providing a visual basis for researchers. A total of 93 compounds were identified, including 52 compounds from the Northeast China, 51 from the Xinjiang region, and 37 from Inner Mongolia region. Then, the in vitro antioxidant activity of sea buckthorn fruit oil was measured using DPPH, ABTS, and SOD inhibition tests, and the results showed that sea buckthorn fruit oil in northeast China was the strongest antioxidant, followed by Inner Mongolia and Xinjiang. The results of the CCK-8 experiment indicated that within the tested concentration, there is no cell cytotoxicity of the essential oil in human umbilical vein endothelial cells (HUVECs) cells. The results could supply reference to distinguish sea buckthorn fruit from different production areas and, meanwhile, clarify the activity and safety of sea buckthorn oil.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xian He,Wanshui Yang,Qihong Zhao,Xinsheng Qin
In this study, we prepared Pickering emulsions stabilized by quinoa protein isolate (QPI) and (-)-epigallocatechin-3-gallate (EGCG) non-covalent hybrid particles using ultrasonic emulsification technique and demonstrated lipid oxidation and in vitro digestion process of Pickering emulsions. The interaction forces between QPI and EGCG were characterized using fluorescence spectroscopy, isothermal titration calorimetry, and Fourier transform infrared spectroscopy. Results indicated that the non-covalent QPI/EGCG hybrid particles were formed mainly via hydrophobic interactions, hydrogen bonds, and electrostatic interactions at pH 5. Then, the QPI/EGCG non-covalent hybrid particles were applied to modify the Pickering emulsion with ultrasonic homogenization. The rheological experimental results showed that the energy storage modulus (G′) was higher than the loss modulus (G″), indicating that the emulsion had solid-like properties. As a physical barrier, interfacial layer fabricated by antioxidant QPI/EGCG hybrid particles limited lipid oxidation at 60 °C for 15 days. At 37 °C, the QPI/EGCG hybrid particles stabilized Pickering emulsions with robust antioxidant interfacial structure limited the lipid digestion under simulated gastrointestinal tract (gastric, small intestine phases). Thus, EGCG and quinoa proteins were more resistant to free radical oxidation and gastrointestinal digestion with the assistance of ultrasound. It provides a basis for better development of food and drug delivery systems by fully utilizing the antioxidant properties of plant polyphenols.
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高影响因子
iScience
·
2024
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IF 4.6
Xiaoyin Rong,Ruixiao Li,Tianye Gong,Haonan Li,Xiaolei Zhao,Guoqing Cao,Meng Li,Bugao Li,Yang Yang,Xiaohong Guo
Circular RNA is a special category of non-coding RNA that has emerged as epigenetic regulator of adipose tissue development. However, the mechanism governing intramuscular adipogenesis of circRNA remains largely uncharted. In this study, circMEF2C(2, 3), looped by MEF2C exons 2 and 3, was identified from the pig MEF2C gene. Expression of circMEF2C(2, 3) is upregulated in early stage of intramuscular adipogenesis and muscular tissue of lean pigs (DLY pig). Subsequently, overexpression or knockdown of circMEF2C(2, 3) reflected that it participates in promoting proliferation and inhibiting adipogenic differentiation in porcine intramuscular preadipocytes and murine C3H10T1/2 cells. Mechanically, circMEF2C(2, 3) competitively combined with miR-383 and miR-671-3p to the 3′-UTR of MEF2C , which maintains MEF2C expression in regulating proliferation and adipogenesis. In summary, circMEF2C(2, 3) is a key regulator in the proliferation and adipogenic differentiation of intramuscular adipogenesis, suggesting its potential as a multi-target strategy for adipose development and associated diseases.
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高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Sisi He,Yiyi Liu,Chaoxian Chi,Dongdong Zhou,Chuan-Fan Ding,Xucong Lin,Jiabin Wang,Fangling Wu
The differentiation of 3-AminoPyrrolidine (3AP) and its derivative isomers is significant due to their use as intermediates in drug synthesis. However, the separation of these isomers, which exhibit both chiral and positional isomerism, has consistently posed challenges. In this study, an efficient analytical method was developed to discrimination between the four isomers of R/S-I/N-Boc-3-AminoPyrrolidine (R/S-l/N-Boc-3AP) and four groups of derived chiral isomers, namely R/S-N-Methyl-3-AminoPyrrolidine (R/S-N-Me-3AP), R/S-N-Ethyl-3-AminoPyrrolidine (R/S-N-Et-3AP), R/S-1-Bzl-3-AminoPyrrolidine (R/S-1-Bzl-3AP), and R/S-1-Cbz-3-AminoPyrrolidine (R/S-1-Cbz-3AP). The discrimination was achieved by utilizing natamycin (Nat) as a differentiation agent and 2/3/4-aminobenzenesulphonic acid (2/3/4-ABSA) as ligand. The facile discrimination of the four positional isomers of R/S-1-Boc-3AP and R/S-N-Boc-3AP was achieved using ion mobility spectrometry (IMS) based on their formed diastereomeric complexes [Nat + (Boc-3AP) 2 + (2-ABSA) 2 + H] + . The isomers were also studied via tandem mass spectrometry (MS/MS) using the formed ternary complexes, different fragment ions were observed for l-Boc-3AP and N-Boc-3AP, but the R/S enantiomers cannot be discriminated either. In addition, the enantiomers R/S-l-Bzl-3AP and R/S-l-Cbz-3AP were differentiated based on mobility using [Nat + (R/S-l-Bzl-3AP) + (3-ABSA) 2 + H] + and [Nat + (R/S-l-Cbz-3AP) + 2-ABSA + H] + ; however for the small enantiomeric molecules of R/S-N-Me-3AP and R/S-N-Et-3AP, chiral discrimination was not achieved via the ternary complexes of Nat + R/S-N-Me-3AP-ABSA and Nat + R/S-N-Et-3AP-ABSA. Importantly, the separation was greatly improved by the coordinated alkali metal ions, where the separation resolution (R p-p ) increased with the radius of the metal ions. Finally, quantitative analysis and method validation were carried out for the four Boc-3AP isomers using both MS/MS and IMS-MS. Overall, the proposed method opens possibilities for the rapid discrimination and quantification of isomers exhibiting both chiral and positional isomerism in MS/MS and IMS-MS analyses.
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Journal of Materials Chemistry C
·
2021
·Vol. 9
·期 15
·5182-5189
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IF 7.393
Liang Bao,Wenqiang Liu,Yonghua Chen,Yanbo Zhang,Yang Zhang
The local surface plasmon resonance effect induced by metal nanoparticles on perovskites provides a powerful approach to enhance the photoluminescence of such all-inorganic systems. Here, we show that the decoration of CsPb(Br/Cl)3 nanocrystals (NCs) with Au nanoparticles (NPs) leads to a significant enhancement of the blue emission centered at 465 nm, resulting in a photoluminescence quantum yield (PLQY) of up to 73%. Our simulations based on finite difference time domain (FDTD) analysis indicate that the distance between the Au NPs and CsPb(Br/Cl)3 NCs plays a crucial role in determining the local electric field intensity. Our data suggest that an excessive decoration of CsPb(Br/Cl)3 NCs with Au NPs can also reduce the PLQY, as a result of decreased coupling strength.
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2021
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IF 5.194
Min Wang,Longji Fang,Tongtong Liu,Xuejie Chen,Yan Zheng,Yilong Zhang,Shiming Chen,Zeng Li
The development of multi-target-directed ligands (MTDLs) may improve complex central nervous system diseases such as Alzheimer's disease (AD). Here, a series of 7-O-1, 2, 3-triazole hesperetin derivatives was evaluated for their inhibition of cholinesterase , anti-neuroinflammatory, and neuroprotective activity. Among the hesperetin derivatives, compound a8 (7-O-((1-(3-chlorobenzyl)-1H-1,2,3-triazol-4-yl)methyl)hesperetin) possessed excellent anti-butyrylcholinesterase activity (IC 50 = 3.08 ± 0.29 μM) and exhibited good anti-neuroinflammatory activity (IC 50 = 2.91 ± 0.47 μM) against NO production through remarkably blocking the NF-κB signaling pathway and inhibiting the phosphorylation of P65. In addition, a8 showed a remarkable neuroprotective effect and lacked neurotoxicity up to 50 μM concentration. Furthermore, possessing significant self-mediated Aβ 1-42 aggregation inhibitory activity, chelated biometals and reduced ROS production were found in compound a8 . In the bi-directional transport assay, a8 exhibited a blood–brain barrier penetrating ability. In this study, the Morris water maze task showed that compound a8 significantly improved the learning and memory impairment of the scopolamine-induced AD mice model. Results highlighted the potential of compound a8 to be a potential MTDL for the development of anti-AD agents.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Cuiwen Jiang,Feiyan Yan,Yuanxin Qin,Jing Liang,Liping Xie,Yanli Wang,Tao Li,Jing Wang,Lufei Zheng,Yu Ya
In this study, a stable and sensitive acetylcholinesterase biosensor was developed for the electrochemical determination of 3-nitropropionic acid (3-NPA). To this end, NaOH etching was used to activate the surface of the glassy carbon electrode, and the morphologies and electrochemistry properties of the resulting electrodes were characterized by various analytical methods. The results indicated dramatic enhancement in the electrochemical properties of electrodes by NaOH etching. As a result, the etched electrodes were applied as novel platforms for the immobilization of acetylcholinesterase. Accordingly, a sensor based on inhibition of 3-NPA toward the activity of acetylcholinesterase was constructed for the electrochemical determination of 3-NPA. Under the optimal conditions, the inhibition rate was found to be proportional to the logarithm of the concentration of 3-NPA from 0.1 to 30 μg/L, and the limit of detection was recorded as 0.05 μg/L. In comparison to conventional detection method, the fabricated biosensor also has added advantages in the form of easy and convenient operation, high sensitivity and better linear range.
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JOURNAL OF SEPARATION SCIENCE
·
2021
·Vol. 44
·期 19
·3646-3653
·
IF 3.645
Mingxia Liu,Lixia Chen,Xiangjun Li,Jinghua Meng,Yu Bai,Huwei Liu
A chiral analytical method was proposed based on capillary electrophoresis with laser-induced fluorescence detection coupled with microwave-assisted derivatization for the simultaneous baseline separation and sensitive detection of four stereoisomers of 3-hydroxyaspartate. The derivatization reaction of 3-hydroxyaspartate with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole was greatly accelerated by microwave irradiation. Under the optimized conditions, the derivatization yield was increased by 20% and the derivatization time was shortened by 20 min when compared with those from conventional water bath heating. In addition, the sensitivity was improved by online sample concentration methods. The detection limit of l - threo -3-hydroxyaspartate obtained by large-volume sample stacking with polarity switching was 5.3 nmol/L, which was around 1000-fold lower than that of the capillary electrophoresis/laser-induced fluorescence without stacking. The excellent analytical performance in terms of linearity and precision was also achieved. Furthermore, the developed method was successfully applied to the determination of 3-hydroxyaspartate in the spiked urine, and satisfactory recoveries were obtained ranging from 90.5 to 107.0%.
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Arabian Journal of Chemistry
·
2021
·
IF 5.165
Aka Alla Martin,Essy Kouadio Fodjo,Zran Vanh Eric-Simon,Zhen Gu,Guangxin Yang,Trokourey Albert,Cong Kong,Hui-Feng Wang
A highly sensitive modified gold (Au) electrode using cysteine-coated silver nanoparticles (Cys-AgNPs) is designed. The electrochemical activity of this designed sensor has exhibited an excellent response for the detection of 3-chloropropane-1,2-diol (3-MCPD). Indeed, the investigation of electrochemical behavior of 3-MCPD at the Cys-AgNPs/Au electrode has shown that the electrocatalytic activity for the 3-MCPD oxidation in NaOH solution is enhanced by three-fold with the presence of Cys-AgNPs film. This Cys-AgNPs film is found to be stable on the Au electrode and the developed electrode can be reused several times while obtaining excellent results. Furthermore, using differential pulse voltammetry technique, the oxidation current peak shows a linear relationship with 3-MCPD concentration between 2.5 ng/mL and 200 ng/mL. The calculated limit of detection (LOD) and limit of quantification (LOQ) are found to be 2.4 ng/mL and 8.6 ng/mL, respectively. A recovery rate ranging from 96% to 105% in the presence of interfering compounds with similar structure to 3-MCPD or not are observed for the determination of 3-MCPD. Moreover, the developed sensor is successfully applied to the determination of 3-MCPD in smoked mackerel and palm oil samples with results which show 3-MCPD concentration ranging from 174 to 439 μg/kg and 895 to 1280 μg/kg in smoked mackerel and palm oil samples, respectively. In addition to the reusability and stability of the electrode, these results suggest that this Cys-AgNPs/Au electrode can be used as an effective sensor to monitor and determine trace level of 3-MCPD in foodstuffs.
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ENZYME AND MICROBIAL TECHNOLOGY
·
2021
·
IF 3.493
Dong-Xu Jia,Chen-Yi Sun,Yi-Ting Jin,Zhi-Qiang Liu,Yu-Guo Zheng,Mian Li,Hong-Yan Wang,De-Shui Chen
Allulose is an epimer of d -fructose with multiple nutritional functions, which can be biologically synthesized from d -fructose employing d -allulose 3-epimerase (DAE). In this study, NCBI database mining approach was applied to screen novel DAEs, and Novibacillus thermophilus DAE (NtDAE) exhibiting a high activity of 273.9 U/(g⋅wet cell weight) was selected for investigation. NtDAE showed optimum reaction temperature and pH at 70 °C and 7.0, respectively. In the presence of Co 2+ , its activity was improved by 100.1 %, and the half-life ( t 1/2 ) values reached up to 846.3, 539.2 and 47.8 min at 40, 50 and 60 °C, respectively. Higher k cat / K m values toward d -allulose indicated that the preferred physiological substrate of NtDAE was d -allulose. The conversion yield of 500 g/L d -fructose to d -allulose at 60 °C achieved 29.7 %, and further enhanced to 51.6 % with adding 1.1 M borate. This study provided a useful guidance of further engineering and application of NtDAE for realization of industrial production of d -allulose.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 19
·8608-8618
·
IF 3.591
Min Chen,Haimei Li,Xueming Su,Runqiang Wu,Huafu Feng,Xiaohang Shi,Jintao Liang,Jiejing Chen,Guiyin Li
Glypican-3 (GPC3) is a potential hepatocellular carcinoma (HCC) serum marker with good sensitivity and specificity. Herein, a label-free electrochemical aptasensor for the detection of GPC3 was developed using a reduced graphene oxide–hemin–chitosan (RGO–H–CS) nanocomposite-modified screen-printed electrode (SPE) as a biosensing platform and GPC3 aptamer as the recognition element. When GPC3 was present, the aptamer could specifically bind with the target GPC3 by specific recognition reaction and form GPC3–aptamer conjugations on the sensing surface, which could alter the electrochemical redox signal of hemin (Fe(III)/hemin(Fe(II))) in the RGO–H–CS nanocomposite recorded by differential pulse voltammetry (DPV). The electrochemical GPC3 aptasensor was characterized via scanning electron microscopy (SEM), Raman spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimal conditions, currents originating from the oxidation of hemin had a linear relationship with the concentration of GPC3 in the range of 0.01 µg mL−1 to 10.0 µg mL−1 with a detection limit of 7.9 ng mL−1 (S/N = 3). The developed aptasensor was applied to detect the GPC3 level in the human serum samples with the recovery rate of 101.04–104.9%. Moreover, the label-free aptasensor exhibited considerable anti-interfering ability, acceptable stability and good reproducibility. Therefore, this study provides a reliable and objective analysis method to achieve high screening for the early diagnosis of HCC.
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危险品化学品经营许可证（带存储）