Nature Nanotechnology
·
2023
·
IF 38.3
Luo Zhenyu,Lu Yichao,Shi Yingying,Jiang Mengshi,Shan Xinyu,Li Xiang,Zhang Junlei,Qin Bing,Liu Xu,Guo Xuemeng,Huang Jiaxin,Liu Yu,Wang Sijie,Li Qingpo,Luo Lihua,You Jian
Pharmaceuticals have been developed for the treatment of a wide range of bone diseases and disorders, but suffer from problematic delivery to the bone marrow. Neutrophils are naturally trafficked to the bone marrow and can cross the bone marrow–blood barrier. Here we report the use of neutrophils for the targeted delivery of free drugs and drug nanoparticles to the bone marrow. We demonstrate how drug-loaded poly(lactic-co-glycolic acid) nanoparticles are taken up by neutrophils and are then transported across the bone marrow–blood barrier to boost drug concentrations in the bone marrow. We demonstrate application of this principle to two models. In a bone metastasis cancer model, neutrophil delivery is shown to deliver cabazitaxel and significantly inhibit tumour growth. In an induced osteoporosis model, neutrophil delivery of teriparatide is shown to significantly increase bone mineral density and alleviate osteoporosis indicators.
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WOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 57
·期 3
·583-603
·
IF 3.4
Yu Fanjun,You Ziyuan,Ma Yushen,Liu Haoyang,Wang Yonggui,Xiao Zefang,Xie Yanjun
A novel, environmentally friendly, biomass-based modification technique utilizing carboxymethylation-activated alkali lignin (CAAL) and glutaraldehyde (GA) is proposed to enhance the physical and mechanical properties of poplar and fir wood. Water-soluble CAAL was prepared by modifying alkali lignin (AL) with sodium chloroacetate following low-temperature activation under a NaOH/urea system. AL is a biomass-based material from industrial by-products. As such, it does not rely on fossil resources nor emit volatile compounds that might limit its range of application. The successful preparation of CAAL was demonstrated by Fourier transform infrared spectroscopy (FTIR), 1 H nuclear magnetic resonance spectroscopy (NMR), and 13 C NMR, and the changes in structure were analyzed by 2D-heteronuclear singular quantum correlation (2D-HSQC), 31 P NMR, and gel permeation chromatography (GPC). The following characteristics of the modified wood samples were investigated in detail: weight percent gain (WPG), bulking, anti-swelling efficiency (ASE), water absorption rate (WAR), leachability of the modifier, microstructure, and mechanical properties. Water immersion results demonstrated that the CAAL/GA hybrid modifier enhanced ASE to 41.3% and 31.5% for fir and poplar, respectively, at a concentration of 15 wt.%. Furthermore, the WAR of fir was reduced by nearly three times to 30.7%, compared to that of a control sample treated solely with GA. GA-treated samples showed high brittleness, whereas both poplar and fir showed enhanced modulus of rupture and elasticity following treatment with the CAAL/GA hybrid modifier.
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Journal of Inorganic and Organometallic Polymers and Materials
·
2023
·Vol. 33
·期 7
·1817-1829
·
IF 4
Qin Yuan,Meng Fuliang,Xu Chunyu,Hu Zhenguo,Zhang Yimiao,Jia Yufei,Li Songjun,Yuan Xinhua
Low toxicity, environmentally friendly and sustainable bio-based phenol–formaldehyde (PF) resins are the primary factors and health goals that researchers need to consider when modifying PF resins. Two novel biomass-modified PF resins were synthesized using two flavonoid phenols of daidzein and naringenin with rigid backbone structures. The results show that compared with ordinary PF, the introduction of daidzein and naringenin during the synthesis of N-PF and D-PF can delay the curing reaction and results in higher curing peak temperatures. The appropriate substitution rate of daidzein and naringenin can improve the crosslinking degree, resulting in N-PF and D-PF with higher thermal stability, ablation resistance and mechanical properties. The highest carbon yield YC800 for N-PF is 59.81% (56.85%for PF-1), and the highest YC800 for D-PF is 64.39% (PF-2 with 58.15%). The maximum tensile strength and flexural strengths of N-PF are respective 33.86 MPa and 110.42 MPa (28.77 and 79.89 MPa for PF-1), and the maximum tensile strength and flexural strengths of D-PF are respective 35.61 MPa and 103.17 MPa (24.48 and 55.79 MPa for PF-2). The D-PF and N-PF resins modified and enhanced by daidzein and naringenin have lower friction coefficient and more excellent wear resistance than pure PF.
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MOLECULAR NEUROBIOLOGY
·
2023
·Vol. 60
·期 7
·3824-3838
·
IF 5.1
Deng Ling,Zhang Jiyu,Chen Sha,Wu Yu,Fan Xiaomei,Zuo Tianrui,Hu Qingwen,Jiang Lu,Yang Shaonan,Dong Zhi
Blood-brain barrier (BBB) disruption can induce further hemorrhagic transformation in ischemic stroke (IS). miR-671-5p, a micro-RNA, is abundant in the cortex of mammalian brains. Herein, we investigated the roles and potential mechanisms for the effects of miR-671-5p on BBB permeability in IS. Results showed that miR-671-5p levels were significantly downregulated in the cerebral cortex of middle cerebral artery occlusion/reperfusion (MCAO/R) C57/BL6 mice in vivo . miR-671-5p agomir administration via right intracerebroventricular injection significantly reduced infarct volume, improved neurological deficits, the axon of neurons and nerve fiber, attenuated cell injury and apoptosis, as well as reduced BBB permeability in MCAO/R mice. Treatment with miR-671-5p agomir alleviated tight junction proteins degradation, including claudin, occludin, and ZO-1 in MCAO/R mice, and these effects were reversed following NF-κB overexpression. Bend.3 brain endothelial cells were subjected to oxygen and glucose deprivation/reoxygenation (OGD/R) treatment in vivo , and then miR-671-5p agomir was transfected into the cells. This resulted in reduction of cytotoxicity, improved cell viability, trans-endothelial electrical resistance, reduced fluorescein sodium permeability, and inhibited tight junction degradation in Bend.3 OGD/R cells. However, these effects were reversed following NF-κB overexpression. These results demonstrated that upregulation of miR-671-5p in IS models in vivo and i n vitro alleviated BBB permeability by targeting NF-κB/MMP-9. In summary, miR-671-5p is a potential therapeutic target for protecting BBB permeability in IS to minimize cerebral hemorrhage transformation.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 4
·1-8
·
IF 5.7
Wu Ning-Ning,Chen Lin-Ge,Xiao Min-Zhi,Yuan Rong-Yao,Wang Hai-Bo
A fluorescent method is described for trypsin determination through the strong electrostatic interactions between cationic polyelectrolytes and single-stranded DNA (ssDNA) templated Au nanoclusters (AuNCs). The ssDNA-AuNCs display improved fluorescence emission with excitation/emission maxima at 280/475 nm after being incorporated with poly(diallyldimethylammonium chloride) (PDDA). Fluorescent enhancement is mainly attributed to the electrostatic interactions occurring between PDDA and ssDNA templates. This can make the conformation of the ssDNA templates to change. Thus, it offers a better microenvironment for stabilizing and protecting ssDNA-AuNCs, and results in fluorescence emission enhancement. By using protamine as a model, the method is employed for the determination of trypsin. The assay enables trypsin to be determined with good sensitivity and a linear response ranging from 5 ng⋅mL −1 to 60 ng⋅mL −1 with a 1.5 ng⋅mL −1 limit of detection. It is also extended to determine the trypsin contents in human’s serum samples with recoveries between 98.7% and 103.5% with relative standard deviations (RSDs) between 3.5% and 4.8%. Graphical abstract A novel fluorescent strategy has been developed for of trypsin determination by using protamine mediated fluorescent enhancement of DNA templated Au nanoclusters.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 13
·5788-5807
·
IF 4.5
Wen Zhijie,Fu Hudie,Ye Xuexin,Yang Xuedan,Zhu Shengpeng,Hu Jie,Kang Li,Li Xiaojun,Yang Xinzhou,Hu Yan
Currently, conventional chemotherapy for hepatocellular carcinoma is limited and has serious side effects due to premature release and limited lesion targeting recognition. In this study, to further address the challenges of drug delivery for liver cancer, a novel hybrid lipid–polymer nanocomposite, glycyrrhetinic acid-modified lipogel (GA-SS-LG), was designed to merge the beneficial features of both polymer-based and lipid-based delivery systems in a single nanocarrier, which was proposed as a liposome with a bifunctional ligand as well as a polymer gel core inside in the liposome. The inhibitory effect of the GA-SS-LG on HepG2 cells was studied in vitro by using curcumin (Cur) as a model drug. The experimental results show that the incorporation of the polymer gel core improved the stability of the liposomes and had the ability to enhance drug encapsulation and delay drug release. At the same time, the liposome outer membrane could be modified with functional ligands to deliver drugs accurately. Our system accurately delivers drugs to tumor cells and responds quickly to release the loaded drugs in large quantities. This study improves the antitumor activity of Cur by preparing a lipogel composite system and provides a new formulation that combines both the polymer-based and lipid-based delivery systems in a single nanocarrier for the clinical application of antitumor drugs. Graphical abstract
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JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2023
·Vol. 34
·期 9
·1-10
·
IF 2.8
Dong Shijun,Li Runqiu,Wu Junpeng,Zhong Wenxia,Zhao Ye,Ji Xinran,Zheng Hui,Zheng Peng,He Hong,Zheng Liang
With the development of 5G electronic products, solving electromagnetic pollution becomes an urgent issue for human health. In this work, the electromagnetic performance of W-type hexagonal barium ferrite (BaZn 2 Fe 16−x Ta x O 27 ) was improved by doping with high-valence Tantalum (Ta 5+ ) ions. The dependence of the concentrations of Ta 5+ ions on the microstructure, crystal structure and electromagnetic properties was studied in detail. Structural characterizations confirm that the doping Ta 5+ prefers the 4f2 site, increasing the lattice values and grain size, promoting more Fe 3+ translation into Fe 2+ , which effectively improves the electromagnetic properties including saturation magnetization, complex permittivity and permeability. However, some Ta 2 O 5 was also formed at the doping concentration of x = 0.2 which deteriorates the electromagnetic properties. The BaZn 2 Fe 16−x Ta x O 27 ferrite at x = 0.15 obtains the highest value of saturation magnetization of 92 emu/g, the highest intensity of complex permittivity and permeability, showing a potential application in absorbing material.
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Drug Delivery and Translational Research
·
2023
·Vol. 13
·期 10
·2475-2486
·
IF 5.4
Ou Yuying,Zhu Demiao
Searching for an effective pore-enlarging agent to form mesoporous silica nanoparticles (MSN) with a creative surface frame is of great importance. Herein, several polymers were attempted to be pore-enlarging agents to form seven types of worm mesoporous silica nanoparticles (W-MSN) and analgesic indometacin that exerted functions on inflammatory diseases (breast disease, arthrophlogosis, etc.) was studied to enhance its delivery efficiency. The porous morphology differences between MSN and W-MSN were that MSN had independent mesopores while the enlarged mesopores of W-MSN were interrelated and shaped as a worm. Among all these W-MSN, WG-MSN templated by hydroxypropyl cellulose acetate succinate HG with the highest drug-loading capacity (24.78%), shortest loading time (10 h), drug dissolution improvement of almost 4 times compared to that of the raw drug, and highest bioavailability (5.48 times higher than that of raw drug and 1.52 times higher than that of MSN) was an outstanding drug carrier and can shoulder the mission to deliver drugs with high efficiency. Graphical Abstract
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-6
·
IF 16.6
Zhou Xiaoyu,Wang Zhuofan,Fan Jingjin,Ouyang Zheng
Elucidation of complex structures of biomolecules plays a key role in the field of chemistry and life sciences. In the past decade, ion mobility, by coupling with mass spectrometry, has become a unique tool for distinguishing isomers and isoforms of biomolecules. In this study, we develop a concept for performing ion mobility analysis using an ion trap, which enables isomer separation under ultra-high fields to achieve super high resolutions over 10,000. The potential of this technology has been demonstrated for analysis of isomers for biomolecules including disaccharides, phospholipids, and peptides with post-translational modifications.
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CHINESE JOURNAL OF POLYMER SCIENCE
·
2023
·Vol. 41
·期 11
·1796-1804
·
IF 4.3
Lin Yu-Cai,Chen Feng,Yang Shou-Bin,Gong Shu-Yue,Luo Yu-Xiang,Wei Fang-Fang,Ding Fu-Chuan,Bai Wei-Bin,Jian Rong-Kun
Traditional silicone materials have poor mechanical properties, poor interfacial adhesion and lack the ability to be reprocessed or recycled. Herein, we developed a novel strategy of incorporating dynamic noncovalent bonds (2-amino-4-hydroxy-6-methylpyrimidine (UPy)) into side chain of silicone backbones to construct supramolecular silicone poly(urea-urethane) (SSPu) coatings with excellent mechanical performance, strong interfacial adhesion and multiple time recycling capability. Impressively, the prepared SSPu is endowed with simultaneously enhanced stiffness (272.0±23.2 MPa) and toughness (8.0±2.0 MJm −3 ). Besides, SSPu shows strong interfacial adhesion (up to 9.0±1.3 MPa) to diverse substrate (stainless steel, aluminum, copper, epoxy and glass) with long term stability. Moreover, SSPu exhibits excellent multi-recyclability and reusability without significantly decrease of its performance. In addition to the abovementioned features, the enrichment of siloxane backbones in the surface layer endows SSPu with robust repellency to water/oil. Our strategy provides a powerful route to fabricate a new multifunctional silicone elastomer. It is highly anticipated that our strategy can effectively extend the service life of silicone coating which can be applied in a wide variety of areas including self-cleaning, antifouling.
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Communications Biology
·
2023
·Vol. 6
·期 1
·1-14
·
IF 5.9
Zhu Haixia,Wen Zongzhuang,Zhang Aizhen,Liu Dongyue,Wang Hongxiang,Cheng Yin,Yang Xing,Xiao Yu,Li Jianyuan,Sun Daqing,Wu Bin,Gao Jiangang
Spermatogenesis is an extremely complex process, and any obstruction can cause male infertility. RhoGDIα has been identified as a risk of male sterility. In this study, we generate RhoGDIα knockout mice, and find that the males have severely low fertility. The testes from RhoGDIα −/− mice are smaller than that in WT mice. The numbers of spermatogonia and spermatocytes are decreased in RhoGDIα −/− testis. Spermatogenesis is compromised, and spermatocyte meiosis is arrested at zygotene stage in RhoGDIα −/− mice. Acrosome dysplasia is also observed in sperms of the mutant mice. At the molecular level, RhoGDIα deficiency activate the LIMK/cofilin signaling pathway, inhibiting F-actin depolymerization, impairing testis and inducing low fertility in mouse. In addition, the treatment of RhoGDIα −/− mice with Rac1 inhibitor NSC23766 alleviate testis injury and improve sperm quality by inhibiting the LIMK/cofilin/F-actin pathway during spermatogenesis. Together, these findings reveal a previously unrecognized RhoGDIα/Rac1/F-actin-dependent mechanism involved in spermatogenesis and male fertility.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 3
·1-7
·
IF 5.7
Li Xiang-Ling,Jiang Han,Zhao Lei,Song Tian shun,Xie Jing jing
A versatile DNA nanomachine detection system has been developed via the combination of DNAzyme with catalytic hairpin assembly (CHA) technology for achieving accurate and sensitive detection of lead ions (Pb 2+ ). In the presence of target Pb 2+ , capture DNA nanomachine formed by AuNP and DNAzyme recognized and reacted with Pb 2+ , which yielded an “active” DNAzyme, that induced the cleavage of substrate strand, and then released the initiator DNA (TT) for CHA. With the help of the initiator DNA TT, self-powered CHA was activated to achieve the signal amplification reaction in the detection of DNA nanomachine. Meanwhile, the initiator DNA TT was released and hybridized with the other H1 strand to initiate another CHA, replacement, and turnovers, producing enhanced fluorescence signal of fluorophore FAM (excitation 490 nm/emission 520 nm) for sensitive determination of Pb 2+ . Under the optimized conditions, the DNA nanomachine detection system revealed high selectivity toward Pb 2+ in the concentration range 50–600 pM, with the limit of detection (LOD) of 31 pM. Recovery tests demonstrated that the DNA nanomachine detection system has excellent detection capability in real samples. Therefore, the proposed strategy can be extended and act as a basic platform for highly accurate and sensitive detection of various heavy metal ions. Graphical Abstract
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