INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Min Long,Guomin Wu,Fenghua Tao,Shuai Ma,Xiangyang Dong,Hongbing Deng
Bone tissue engineering scaffolds are an important means of repairing bone defects, but current solutions do not adequately simulate complex extracellular microenvironment fibrous structures and adjustable mechanical properties. We use template-assisted fiber freeze-shaping technology to construct silk fibroin nanofiber aerogels (SNFAs) with nanofibrous textures and adjustable mechanical properties. The parallel arranged channels, the pores, electrospun nanofibers, and silk protein conformation together constitute the hierarchical structure of SNFAs. Especially, the introduced electrospun nanofibers formed a biomimetic nanofibrous texture similar to the extracellular matrix, providing favorable conditions for cell migration and tissue regeneration. In addition, Young's modulus of SNFAs can be adjusted freely between 7 and 88 kPa. The rationally designed 3D architecture makes SNFAs perfectly mimic the fiber structure of the extracellular matrix and can adjust its mechanical properties to match the bone tissue perfectly. Finally, fiber-containing SNFAs observably promoted cell adhesion, proliferation, and differentiation, accelerating the bone repair process. The bone density in the defect area reached 0.53 g/cm 3 and the bone volume/total volume (BV/TV) ratio reached 57 % at 12 weeks, respectively. It can be expected that this kind of tissue engineering scaffold with highly simulating extracellular matrix microenvironment and adjustable mechanical properties will possess broad prospects in the field of bone repair.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Bo Xue,Wen Cao,Hong Zhao,Bingqiang Zhang,Jia Liu,Huixin Zhang,Hongzhao Qi,Qihui Zhou
Developing biomaterials with high osteogenic properties is crucial for achieving rapid bone repair and regeneration. This study focuses on the application of nanocrystal hydroxyapatite (nHAp) as a drug carrier to load Fu Yuan Huo Xue Decoction (FYHXD), a traditional Chinese medicine derived from Angelica sinensis, aiming to achieve improved efficacy in treating bone diseases such as osteoporosis. Through a facile physical adsorption approach, the FTIR result emerges new characteristic absorption peaks in the range of 1200–950 cm −1 , proving the successful absorption of FYHXD onto the nHAp with a loading efficiency of 39.76 %. The modified nHAp exhibits a similar shape to the bone-derived hydroxyapatite nanocrystals, and their diameter increases slightly after modification. The drug release assay implies the rapid release of FYHXD in the first 10 h, followed by a continuously slow release within 70 h. The developed nHAp effectively enhances the adhesion, spreading, and proliferation of MC3T3-E1 cells in vitro, and significantly promotes their osteogenic differentiation, as indicated by increased alkaline phosphatase activity. Overall, the biocomposites hold great promise as active ingredients for integration into bone-associated biomaterials, offering the potential to stimulate spontaneous osteogenesis without requiring exogenous osteogenic factors.
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ANALYTICA CHIMICA ACTA
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2024
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IF 5.7
Jianhui Xie,Shuying Mao,Yanglan Zhao,Guimin Zhang,Jingchun Yao,Yongxia Guan,Jizhong Yan,Hui Zhang
Natural products-based screening of active ingredients and their interactions with target proteins is an important ways to discover new drugs. Assessing the binding capacity of target proteins, particularly when multiple components are involved, presents a significant challenge for sensors. As far as we know, there is currently no sensor that can accomplish high-throughput quantitative analysis of natural product-target protein binding capacity based on Raman spectroscopy. In this study, a novel sensor model has been developed for the quantitative analysis of binding capacity based on Surface-Enhanced Raman Spectroscopy (SERS) and Photocrosslinked Molecular Probe (PCMP) technology. This sensor, named SERS-PCMP, leverages the high throughput of molecular probe technology to investigate the active ingredients in natural products, along with the application of SERS labelling technology for target proteins. Thus it significantly improves the efficiency and accuracy of target protein identification. Based on the novel strategy, quantitative analysis of the binding capacity of 20 components from Shenqi Jiangtang Granules (SJG) to α-Glucosidase were completed. Ultimately, the binding capacity of these active ingredients was ranked based on the detected Raman Intensity. The compounds with higher binding capacity were Astragaloside IV (Intensity, 138.17), Ginsenoside Rh2 (Intensity, 87.46), Ginsenoside Rg3 (Intensity, 73.92) and Ginsenoside Rh1 (Intensity, 64.37), which all exceeded the binding capacity of the positive drug Acarbose (Intensity, 28.75). Furthermore, this strategy also performed a high detection sensitivity. The limit of detection for the enzyme using 0.1 mg of molecular probe magnetic nanoparticles (MP MNPs) was determined to be no less than 0.375 μg/mL. SERS-PCMP sensor integrating SERS labeling and photocrosslinked molecular probes which offers a fresh perspective for future drug discovery studies. Such as high-throughput drug screening and the exploration of small molecule-target protein interactions in vitro.
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ACS Biomaterials Science & Engineering
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2024
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IF 5.4
Hao Jin,Xuanqi Zhu,Heng Liu,Lu Wang,Shuwei Liu,Hao Zhang
The clinical treatment of bone defects includes allogeneic bone transplantation and autologous bone transplantation. However, they all have their own limitations, and the scope of application is limited. In recent years, bone tissue engineering scaffolds based on a variety of materials have been well developed and achieved good bone regeneration ability. However, most scaffold materials always face problems such as high biotoxicity, leading to inflammation and poor bioactivity, which limits the bone regeneration effect and prolongs the bone regeneration time. In our work, we prepared hydroxyapatite, erythropoietin (EPO), and osteogenic growth peptide (OGP) codoped type-I collagen (Col I) polypeptide nanofiber membranes (NFMs) by electrostatic spinning. In cell experiments, the composite NFMs had low cytotoxicity and promoted osteogenic differentiation of rat bone marrow mesenchymal stem cells. Quantitative real-time polymerase chain reaction and alkaline phosphatase staining confirmed the high expression of osteogenic genes, and alizarin red S staining directly confirmed the appearance of calcium nodules. In animal experiments, the loaded hydroxyapatite formed multiple independent mineralization centers in the defect center. Under the promotion of Col I, EPO, and OGP, the bone continued to grow along the mineralization centers as well as inward the defect edge, and the bone defect completely regenerated in about two months. The hematological and histological analyses proved the safety of the experiments. This kind of design to promote bone regeneration by simulating bone composition, introducing mineralization center and signal molecules, can shorten repair time, improve repair effect, and has good practical prospects in the future.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Hongyan huang,Shengji Wu,Hui Wang,Jie Zhou
Thermal pretreatment reduces rather than improves Hg 0 removal of AC. • Acidic functional groups serve as the active centers for Hg 0 oxidation. • Hydrogen-bonded microenvironment enhances catalytic activity of acidic sites. • Synergy of acid sites and microenvironment enables high-efficiency Hg 0 removal. Activated carbon injection is a mainstream technology for elemental mercury (Hg 0 ) removal from flue gas. This study investigates an abnormal phenomenon that thermal pretreatment of AC reduces rather than improves its Hg 0 removal efficiency, and systematically elucidates the underlying mechanism. The results demonstrate that acidic sites (e.g., carboxyl groups) on the AC surface serve as the active centers for the catalytic oxidation of Hg 0 , while the hydrogen-bonded microenvironment formed by adsorbed H 2 O molecules significantly enhances the acidity strength and catalytic activity of the acidic sites. Removal of the hydrogen-bond microenvironment severely reduces Hg 0 removal performance, whereas supplementation of H 2 O or other hydrogen-bond donor molecules effectively recovers the Hg 0 removal efficiency. At medium-to-high temperatures, constructing a thermally stable microenvironment (e.g., using benzoic acid) significantly improves the Hg 0 removal efficiency of AC. Based on this synergistic mechanism, the acid-treated and benzoic acid-modified AC (AC-H-150-benzoic acid) could achieve an initial Hg 0 removal efficiency of 75.8% at 150 °C. This work reveals the synergistic effect between acidic sites and the hydrogen-bonded microenvironment on AC for Hg 0 removal, providing new insights for the rational design of high-performance Hg 0 removal adsorbents. Download: Download high-res image (182KB) Download: Download full-size image
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Sensing and Bio-Sensing Research
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2026
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IF 4.7
Ahmad Movahedpour,Omid Sheydaei,Arash Asfaram,Sajedeh Karami,Hassan Ghasemi
This study presents the design and electrochemical characterization of a highly sensitive and selective sensor for the determination of D−/L- kynurenine (D−/L- KYN), based on a carbon paste electrode (CPE) modified with multi-walled carbon nanotubes (MWCNTs). The electrocatalytic oxidation of D−/L-KYN at the MWCNT-modified CPE was systematically investigated using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The Structural and morphological characteristics of the MWCNTs were characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). The combined characterization results confirmed the successful functionalization, morphological integrity, and crystalline structure of the MWCNTs, supporting their suitability as an efficient electrode modifier. At physiological pH (7.0) in phosphate buffer solution (PBS), D−/L-KYN exhibited a well-defined oxidation peak at +0.85 V (vs. Ag/AgCl). The electrochemical behavior of the analyte followed a diffusion-controlled mechanism involving a two-electron/two-proton transfer process. Key analytical parameters including solution pH and scan rate were optimized to enhance sensor performance. The fabricated sensor exhibited a linear concentration range of 0.06–40.0 μM, with an impressive limit of detection 3 nM (S/N = 3). Furthermore, the sensor demonstrated excellent reproducibility and repeatability, with relative standard deviations (RSDs) of 3.8% and 3.3%, respectively. The developed platform was successfully applied to the quantification of D−/L-KYN in spiked human plasma samples, demonstrating its potential for clinical and biomedical applications.
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Journal of Cleaner Production
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2026
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IF 10.7
Min Zhou,Ningbo Yu,Dongyang Li,Minghao Wang,Hongxia Gao,Zhiwu Liang
Intrinsic CO 2 kinetics of cyclic amines are quantified in water-lean ethers. • Zwitterion mechanism is validated as dominant in aprotic ether-water mixtures. • Ether solvents impede CO 2 absorption by disrupting the dynamic H-bond network. • MD shows an ether-induced solvent cage effect restricting CO 2 access to amines. • Rigid C-substituted amines with high nucleophilicity suit ether-based systems. Piperazine-derived cyclic amines represent promising solutions for efficient CO 2 capture. However, fundamental understanding of their structure-activity relationships remains limited. This work combined experimental and computational approaches to elucidate the influences of cyclic structures and functional groups of piperazine-derived cyclic amines (PZ, 2MPZ, AEP, 1MPZ and 2HPZ) on their CO 2 capture performance and the structure-activity relationship in both pure water and water-lean environments. Key findings reveal that substituents reduce nitrogen electron density and impose steric hindrance, impeding CO 2 access to active sites. Stopped-flow measurements of the intrinsic kinetics of cyclic amines in aqueous and ether-water solvents revealed a reactivity trend: PZ > 2MPZ > AEP > 1MPZ > 2HPZ. The zwitterion mechanism demonstrates relatively good accuracy (AARD: 1.30%-2.66%) than base-catalyzed hydration (AARD: 2.62%-9.46%). DFT validates the zwitterion pathway, while MD simulations indicate that pure water solution maintains dynamic hydrogen-bond networks without outer-layer clustering. Ether addition disrupts hydrogen bonding and diminishes electron density at reactive sites, reducing absorption rates. These findings elucidate the underlying structure-activity relationships and solvation effects, providing a fundamental molecular-level understanding of the kinetic behaviors in water-lean amine systems. Download: Download high-res image (578KB) Download: Download full-size image
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POLYMER
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2026
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IF 5.1
Silu Wang,Yinan Zeng,Ammara Aslam,Haofei Yang,Yu Wang,Runsha Zhai,Yangyang Hu,Jianhua Zhang
The fast sol-gel transition of ABA triblock copolymers in aqueous solution has demonstrated excellent promise for various applications. However, the occurrence of gelation behaviors often needs precise temperature control or concentration regulation. Considering the advantages of precisely spatiotemporal control and noninvasive manner, the photo-controlled gelation behaviors are desirable but still remain a significant challenge, especially for ABA triblock copolymers. Herein, we reported a facile strategy for spatiotemporal photo-modulation of reversible gelation of ABA triblock copolymers. The pH-responsive ABA triblock copolymers [poly( N,N - diethylaminoethyl methacrylate)- b -polyethylene glycol- b -poly( N,N -diethylaminoethyl methacrylate), PDEAEMA-PEG-PDEAEMA] were synthesized and used for preparing photobase generator (Malachite green carbinol base, MGOH)-containing micellar solution. Under ultraviolet irradiation, the MGOH in PDEAEMA-PEG-PDEAEMA micelles could rapidly generate OH − , resulting in deprotonation and hydrophobization of PDEAEMA chain, finally achieving micellar reconstruction and in situ gelation transition. This study demonstrated not only a photo-controlled reversible sol-gel transition of ABA triblock copolymers without the limitations associated with conventional photosensitive reagents, but also a low-cost and fast system for intelligent monitoring, early warning, and rapid shielding of ultraviolet rays. Considering the easily available agents, simple preparation process and high flexibility in tuning hydrogel properties, this strategy opens up a promising avenue to develop photo-controlled hydrogels for various applications without involving complex synthesis of photosensitive polymers.
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MINERALS ENGINEERING
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2026
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IF 6
Xuan Wang,Zongke Zhou,Yi Liu,Wei Sun,Haisheng Han
SDS outperforms amine collectors in glaserite flotation. • SDS chemisorbs on glaserite; OA only physisorbs. • Phase diagram guides closed-loop glaserite-to-K 2 SO 4 conversion. • Dual recycling loops maximize resource use and atom economy. • Sustainable route for salt lake potassium sulfate production. The efficient recovery of glaserite (Na 2 SO 4 ·3K 2 SO 4 ) from salt lake ores and its subsequent conversion to potassium sulfate (K 2 SO 4 ) is crucial for sustainable potash fertilizer production. This study investigates the selective flotation separation of glaserite from sodium sulfate (Na 2 SO 4 ) using sodium dodecyl sulfate (SDS) and octadecylamine (OA) as collectors, and further develops a closed-loop chemical conversion process guided by phase diagram analysis. Micro-flotation tests show that SDS achieves a superior glaserite recovery of 82.4% at a dosage of 120 g/t, significantly outperforming OA (67.7%), while neither collector effectively floats sodium sulfate. Multi-spectral (FTIR) and zeta potential analyses, combined with density functional theory (DFT) calculations, reveal that SDS undergoes strong chemisorption on glaserite surfaces via its sulfonate group with a high adsorption energy of −175.71 kcal/mol, increasing the water contact angle from 32.22° to 63.41°. In contrast, OA exhibits weaker physical adsorption (−33.39 kcal/mol), providing only moderate hydrophobization. Based on the 25 °C phase diagrams of the K + , Na + // Cl⁻, SO 4 2 ⁻–H 2 O quaternary system and the NaCl–KCl–H 2 O ternary system, a closed-loop process was designed comprising three steps: (1) reaction of KCl with glaserite to precipitate K 2 SO 4 ; (2) addition of Na 2 SO 4 to the mother liquor to precipitate glaserite; and (3) removal of residual SO 4 2 ⁻ with CaCl 2 followed by evaporative crystallization of NaCl, with the final mother liquor recycled. Theoretical calculations based on 100 kg KCl yield 154.00 kg K 2 SO 4 , 65 kg glaserite, and 15.3 kg NaCl, with experimental deviations within 3%. The obtained K 2 SO 4 and NaCl purities reach 98.90% and 99.5%. The one‑pass potassium ion extraction rate is 86.46%. This integrated flotation‑conversion strategy provides a sustainable, efficient, and environmentally friendly pathway for comprehensive utilization of salt lake resources.
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CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Ping Hu,Jun Sun,Jialin Li,Aolin Li,Haiming Duan,Zhaofeng Wu
P-doped biomass carbon/SnO 2 heterostructure delivers 32.9k% ethylene glycol response, 0.53 ppm LoD, and sustained performance under 85% RH and 6-month air exposure. • 1D Poisson-based n-n heterojunction model reveals 0.39 eV barrier modulation and carbon-dominated depletion control for signal amplification. • DFT and experiments confirm SnO 2 (110) governs selective adsorption, while P-doped carbon modulates barrier for signal amplification, guiding sensor design. The integration of carbon materials with metal oxide semiconductor (MOS) to engineer heterojunction composites is a proven strategy for enhancing gas-sensing performance. However, the interfacial chemistry and charge transfer mechanisms governing the carbon/MOS interface remain largely elusive. Herein, an n-n heterojunction composite of P-doped biochar/SnO 2 was constructed using cotton stalks and wool as dual-biomass precursors. This system achieves highly efficient detection of ethylene glycol (EG) at room temperature, exhibiting a high response of 32.9k%, a limit of detection of 0.53 ppm, and excellent long-term stability over half a year. Mechanistic studies reveal that the preferentially exposed (110) facets of SnO 2 exhibit a remarkably strong adsorption energy of −2.829 eV for EG, which dictates the gas selectivity of the composite system. More importantly, based on the one-dimensional Poisson's equation, an electrical amplification model coupling heavy-doping and light-doping regions was established. This model clarifies that the biochar side with lower carrier concentration dominates the variation in the depletion layer width. Arrhenius fitting confirms that the introduction of P elements expands the modulation range of the interfacial potential barrier height from 0.03 eV to 0.39 eV. These results define the specific roles of MOS, which govern adsorption selectivity, and biochar, which govern gas-sensing signal amplification, at the physical level. This dual-precursor strategy enables the simultaneous formation of a tubular carbon scaffold and in situ P doping in a single step. The established quantitative physical model demonstrates that the depletion layer is dominated by the lightly doped biochar side. This work provides a mechanistic framework for understanding interfacial engineering in carbon/MOS composites, enabling rational design of advanced heterojunctions through precise control of interface properties and charge transfer kinetics.
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JOURNAL OF MEMBRANE SCIENCE
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2026
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IF 8.8
Xiaozhen Lu,Lin Wang,Bin Liu,Hong Peng,Xuewu Zhu
Nanofiltration (NF) for advanced drinking-water treatment has long been constrained by the need to remove contaminants while preserving beneficial mineral ions in health-oriented drinking water. Herein, an ethylene-glycol (EG)-regulated aqueous microenvironment was introduced to tailor the interfacial polymerization process. EG-mediated hydrogen bonding and modified interfacial thermodynamics jointly regulated piperazine diffusion, interfacial transport, and polymerization kinetics, producing polyamide NF membranes with enhanced negative charge surface density and a more uniform effective pore structure. Compared with the pristine membrane, the EG-20 membrane exhibited a thinner, more hydrophilic, and more negatively charged selective layer, together with a narrower pore-size distribution. The optimized EG-20 membrane achieved a permeance of 23.6 L m −2 h −1 bar −1 while maintaining Na 2 SO 4 rejection above 95.5%. In the treatment of natural surface water, dissolved organic matter removal reached approximately 92.1%, while partial passage of beneficial mineral ions was maintained, enabling effective organic contaminant removal with mineral preservation for health-oriented drinking water treatment. Moreover, high rejection of PFAS and antibiotics was maintained, mainly because steric exclusion and enhanced electrostatic repulsion compensated for the moderately enlarged effective pores. In addition, the membrane showed improved antifouling performance, as enhanced surface hydration and electrostatic repulsion reduced foulant adhesion and suppressed irreversible fouling. Overall, this microenvironment-regulation strategy provides a feasible route for developing NF membranes that integrate effective contaminant control, mineral preservation, and stable operation.
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Journal of Energy Storage
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2026
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IF 10.7
Zesen Yu,Xuan Yang,Pingchun Guo,Hedong Jiang,Jiake Li,Hua Zhu,Xueguo Zhao,Yanxiang Wang
Developing solid-state polymer electrolytes (SPEs) with fast Li + transport and stable electrode interfaces is essential for high-performance lithium metal batteries. Herein, a protonated amino-functionalized metal-organic framework (pro-UiO66) is incorporated into a PEO-LiTFSI matrix to construct composite SPEs. Protonation introduces abundant TFSI − adsorption sites(-NH 3 + ), while the three-dimensional porous framework disrupts PEO crystallinity and provides angstrom-scale pathways for Li + migration. Various characterization confirms that pro-UiO66 maintains crystallinity and uniform dispersion in the polymer matrix. The optimized electrolyte containing 10 wt% pro-UiO66 exhibits reduced crystallinity (29.57%), a high Li + transference number (0.55), and an ionic conductivity of 4.65 × 10 −4 S cm −1 at 60 °C, together with an electrochemical stability window of 5.3 V. Li||Li symmetric cells cycle stably for over 3200 h, while LFP||Li cells deliver 142 mAh g −1 with 92.2% capacity retention after 300 cycles. Mechanistic analysis indicates that the synergistic effects of the porous framework and -NH 3 + functional groups promote LiTFSI dissociation, regulate Li + deposition, and enable the formation of a stable solid electrolyte interphase, thereby suppressing dendrite growth. This work provides insight into protonation-engineered MOFs fillers for designing SPEs toward safe and high-performance solid-state lithium metal batteries.
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