ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Pan-Pan Jia,Tai Sun,Muhammad Junaid,Li Yang,Yan-Bo Ma,Zhi-Song Cui,Da-Peng Wei,Hao-Fei Shi,De-Sheng Pei
Graphene family nanomaterials (GFNs) have attracted significant attention due to their unique characteristics and applications in the fields of biomedicine and nanotechnology. However, previous studies highlighted the in vitro and in vivo toxicity of GFNs with size and oxidation state differences are still elusive. Therefore, we prepared graphene (G) and graphene oxide (GO) of three different sizes (S-small, M-medium, and L-large), and characterized them using multiple surface-sensitive analytical techniques. In vitro assays using HEK 293T cells revealed that the small and large sizes of G and GO significantly reduced the cell viability and increased DNA damage, accompanying with activated reactive oxygen species (ROS) generation and induced various expressions of associated critical genetic markers. Moreover, the bacterial assays highlighted that G and GO caused strong acute toxicity on Tox2 bacteria. Effects of G were higher than GO and showed size dependent effect: L > M > S, while the medium size of GO induced mild genetic toxicity on RecA bacteria. In vivo assays revealed that exposure to G and GO caused the developmental toxicity, induced ROS generation, and activated related pathways (specifically GO) in zebrafish. Taken together, G showed stronger ability to decrease the survival rate and induce the acute toxicity, while GO showed obvious toxicity in terms of DNA damages, ROS generation, and abnormal gene expressions. Our findings highlighted that G and GO differentially induced toxicity based on their varying physical characteristics, especially sizes and oxidation state, and exposure concentrations and sensitivity of the employed in vitro and in vivo models. In short, this study provided deep insights on the negative effects of GFNs exposure.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Da-Qi Cao,Wen-Yu Yang,Zhen Wang,Xiao-Di Hao
Traditional wastewater treatment plants cannot completely remove trace pharmaceutical and personal care products. In particular, quinolone antibiotics are mainly adsorbed on microbial cell surfaces in excess sludge and are released into the water environment during agricultural supplementation, mainly due to the exfoliation of extracellular polymeric substances (EPS) caused by changes in environmental conditions. Here, the occurrence of seven typical quinolone antibiotics from three generations in excess sludge was investigated at trace concentrations using ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). EPS facilitated adsorption independent of pH and quinolone antibiotic type, and the solid-liquid distribution coefficient decreased markedly as pH increased (pH 5–9, range of municipal wastewater). Metal ions bound in EPS were predominantly responsible for the adsorption of antibiotics in sludge, rather than the macroscopic size of the sludge floc. pH affected patterns of quinolone antibiotics, the zeta potential of sludge floc, and the contents of metal ions contained in sludge. The adsorption capacity of antibiotics first increased and then decreased with increasing pH in the range of pH 3–11, reaching a maximum (20,506, 14,458, 10,689, 22,854, 20,302, 8494, and 29,547 L/kg for CIP, ENR, LOM, MOX, NOR, OFL, and SAR, respectively) near pH 5, at which the cationic bridging of Ca 2+ and Mg 2+ bound in EPS played a major role owing to their larger ion radius and higher contents in excess sludge. Due to high surface heterogeneity of sludge, the Freundlich model was more suitable than the Langmuir model for describing the adsorption behavior of quinolone antibiotics. These results provide further insight into the release of quinolone antibiotics adsorbed in excess sludge, with potential implications for the agricultural use of sludge.
✓ 已验证
产品关联
DOI
高影响因子
Molecular Catalysis
·
2019
·
IF 2.938
Zhijie Wang,Anxiang Guan,Mayfair C. Kung,Anyang Peng,Harold H. Kung,Ximeng Lv,Gengfeng Zheng,Linping Qian
Aerobic oxidation of α-C H bond of organic compounds to valuable chemicals is widely investigated in both fundamental research and industry. Due to the good stability of molecular oxygen, severe reaction conditions are generally required. Herein, by in situ synthesis we used molecular oxygen to induce cobalt nanoclusters with the sensitive catalysis in mild selective oxidation. The cobalt containing clusters with an average diameter around 0.9 nm are in situ prepared in the presence of cis -cyclooctene epoxidation and cyclooctene dimer oxide is formed at the interface to stabilize Co clusters with electron donation as an oil-soluble surfactant. The soluble clusters exhibit high activity in selective oxidation of α-C H bond of ethylbenzene into acetophenone and turnover number (TON) reaches about 7 × 10 4 during 50 h’ reaction at 373 K, which is around 960 times more active than the one using CoCl 2 salt as the catalyst, resulting from efficient mass transportation, π bond interaction and oxygen gas activation. Extended work based on this understanding demonstrates that cobalt nanoclusters also effectively catalyze aerobic oxidation of cyclohexene.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 16
·9006-9013
·
IF 3.049
Shengli Mi,Jingjing Xia,Yuanyuan Xu,Zhichang Du,Wei Sun
In this study, we developed an electrochemical microchannel biosensor platform to analyse lactate metabolism in cells. This biosensor platform was fabricated by photolithography, thin-film deposition and microfluidic technology. A kind of functional biomaterial was prepared by mixing lactate oxidase, single-walled carbon nanotubes and chitosan, and platinum as working and blank electrodes of the biosensor was modified by a thin Prussian blue layer. The lactate biosensor was obtained by dropping functional biomaterials on the electrode. The results demonstrated that the sensitivity of the electrochemical biosensor was up to 567 nA mM−1 mm−2 and the limit of detection was 4.5 μM (vs. Ag/AgCl as the counter/reference electrode). The biosensor used to quantitatively detect metabolic lactate concentrations in HepG2 cells cultured with cancer drugs showed high sensitivity, selectivity and stability, and has potential applications in organ-on-a-chip and tissue engineering technologies, which typically involve low concentrations of metabolites.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2019
·
IF 3.261
Han Zhang,Mei Zhu,Dao-Yong Jiang,Xing-Ying Xue,Jiao Zhang,Guo-Ning Zhang,Yu-Cheng Wang,Hong Zhao
A NIR fluorescent probe AZ-NO 2 for hydrogen sulfide was designed and synthesized through simple method, which employs dicyanoisophorone based fluorescent dye as a fluorophore and 2,4-dinitrophenyl ether moiety as the specific reaction site. Owing to the H 2 S-induced efficient thiolysis of dinitrophenyl ether, the probe displayed excellent selectivity toward H 2 S, a low detection limit of 16.3 nM, a large Stokes shift (185 nm) and an ultrafast response time (75 s). In addition, the sensing mechanism was investigated via mass spectra. Finally, in view of its favourable biocompatibility, the probe AZ-NO 2 has been successfully applied to the fluorescent bioimaging of H 2 S in HepG2 cells and zebrafish.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
·
2019
·Vol. 43
·期 5
·2103-2117
·
IF 2.928
Nan Miao,Yuanbo Zhan,Yingying Xu,Haoze Yuan,Chunlin Qin,Feng Lin,Xiaohua Xie,Sen Mu,Mengtong Yuan,Haibin Mu,Shouli Guo,Ying Li,Bin Zhang
Family with sequence similarity 20‑member C (FAM20C), a recently characterized Golgi kinase, performs numerous biological functions by phosphorylating more than 100 secreted proteins. However, the role of FAM20C in the salivary glands remains undefined. The present study demonstrated that FAM20C is mainly located in the cytoplasm of duct epithelial cells in the salivary glands. Fam20cf/f; Mmtv‑Cre mice were created in which Fam20c was inactivated in the salivary gland cells and observed that the number of ducts and the ductal cross‑sectional area increased significantly, while the number of acinar cells was reduced. The granular convoluted tubules (GCTs) exhibited an accumulation of aberrant secretory granules, along with a reduced expression and altered distribution patterns of β nerve growth factor, α‑amylase and bone morphogenetic protein (BMP) 4. This abnormality suggested that the GCT cells were immature and exhibited defects in developmental and secretory functions. In accordance with the morphological alterations and the reduced number of acinar cells, FAM20C deficiency in the salivary glands significantly decreased the salivary flow rate. The Na+, Cl– and K+ concentrations in the saliva were all significantly increased due to dysfunction of the ducts. Furthermore, Fam20c deficiency significantly increased BMP2 and BMP7 expression, decreased BMP4 expression, and attenuated the canonical and noncanonical BMP signaling pathways in the salivary glands. Collectively, the results of the present study demonstrate that FAM20C is a key regulator of acinar and duct structure and duct maturation and provide a novel avenue for investigating novel therapeutic targets for oral diseases including xerostomia.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2019
·Vol. 54
·期 11
·8216-8225
·
IF 3.442
Xu Wen,Cao Bo,Lin Hechun,Cheng Wenjuan,Luo Chunhua,Peng Hui,Duan Chungang
The catalytic activities of three topotactic phase strontium cobaltites (SCO, hexagonal, orthorhombic and tetragonal) for H 2 O 2 decomposition were studied. All they present excellent catalytic activity with good stability at cycled test, and the mesoporous tetragonal SCO exhibits the best activity. Detailed kinetic research indicates the zero-order process of H 2 O 2 decomposition. Increasing the temperature will accelerate the reactions greatly with the controlled temperature in the range of 20–45 °C. The calculated active energy ( E a ) for H-SCO, O-SCO and T-SCO is 93.2, 85.1 and 87.5 (kJ/mol), respectively. The efficient catalytic degradation of Rhodamine B illustrates that SCO is a kind of promising catalysis for the advanced oxidation process.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Qi Wenbo,Xu Lijian,Zhao Shuangdan,Zheng Jiahua,Tian Yanpeng,Qi Xuejun,Huang Xianghua,Zhang Jingkun
Herein we report a facile approach for chitosan-heparin hydrogels with controlled release manner and their applications for intrauterine adhesion. The sol precursor was converted to gel at physiological temperature in 15 min. FTIR, SEM and swelling test were performed to characterize their compositions, morphologies and stability. In vitro releasing profiles was investigated in PBS solutions. Intrauterine injured rat model was established and treated with different methods. The results of H&E staining, Masson trichrome staining, western blots assay, immunohistochemical staining and immunofluorescence staining revealed that endogenous c-kit positive stem cells (HSCs) were recruited to the injury site and promoted the wound recovery. After 7 days' treatment, uterus treated with SDF-1α releasing hydrogel showed no difference with control group on endometrial thickness, glands number and fibrosis level. This work provides a possible method for intrauterine adhesion healing.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 20
·期 17
·4111
·
IF 4.183
Kangfeng Cai,Huaizhou Gao,Xiaojian Wu,Shuo Zhang,Zhigang Han,Xiaohui Chen,Guoping Zhang,Fanrong Zeng
In this work, the effect of drought on K+uptake in root and its translocation from root to shoot was investigated using six barley genotypes contrasting in drought tolerance. Results showed that drought conditions caused significant changes in K+uptake and translocation in a time- and genotype-specific manner, which consequently resulted in a significant difference in tissue K+contents and drought tolerance levels between the contrasting barley genotypes. The role of K+transporters and channels and plasma membrane (PM) H+-ATPase in barley’s adaptive response to drought stress was further investigated at the transcript level. The expression of genes conferring K+uptake (HvHAK1,HvHAK5,HvKUP1,HvKUP2andHvAKT1) and xylem loading (HvSKOR) in roots were all affected by drought stress in a time- and genotype-specific manner, indicating that the regulation of these K+transporters and channels is critical for root K+uptake and root to shoot K+translocation in barley under drought stress. Furthermore, the barley genotypes showed a strong correlation between H+efflux and K+influx under drought stress, which was further confirmed by the significant up-regulation ofHvHA1andHvHA2. These results suggested an important role of plasma membrane H+-ATPase activity and/or expression in regulating the activity of K+transporters and channels under drought stress. Taken together, it may be concluded that the genotypic difference in drought stress tolerance in barley is conferred by the difference in the ability to regulate K+transporters and channels in root epidermis and stele.Keywords:drought tolerance;ion flux;transmembrane K+transport;plasma membrane H+-ATPase;barley
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Energy Storage Materials
·
2018
·
IF 0
Jieqiong Qin,Sen Wang,Feng Zhou,Pratteek Das,Shuanghao Zheng,Chenglin Sun,Xinhe Bao,Zhong-Shuai Wu
Two-dimensional (2D) mesoporous nanosheets as electrodes present a critical materials platform for dramatically boosting the performance of planar micro-supercapacitors (MSCs) due to their unique features of interconnected porous network, enriched nanopore arrays, and high specific surface area. However, efficient strategies for constructing such complicated mesoporous architectures are very limited. Here, we developed a supramolecular bottom-up self-assembly strategy for direct synthesis of ultrathin mesoporous manganese dioxide (m-MnO 2 ) nanosheets for high-energy all-solid-state planar asymmetric MSCs (AMSCs). The m-MnO 2 nanosheets exhibited ultrathin thickness of 10 nm, uniformly interconnected network of mesopores with size of 5–15 nm, high surface area of 128 m 2 g −1 , and remarkably enhanced capacitance of 243 F g −1 at 1 mV s −1 in comparison with non-mesoporous MnO 2 nanosheets (123 F g −1 ). Further, all-solid-state AMSCs were assembled based on m-MnO 2 nanosheets as positive electrode , porous VN nanosheets as negative electrode , and electrochemically exfoliated graphene as conductive agent and metal-free current collector in “water-in-salt” gel electrolyte. Importantly, our all-solid-state AMSCs operated stably at 2.0 V and offered impressive energy density of 21.6 mWh cm −3 , outperforming most reported MnO 2 based MSCs, and two times higher than lithium thin-film batteries (≤10 mWh cm −3 ). Also, they presented long-term cycling stability with 90% capacity retention after 5000 cycles, outstanding flexibility without observable capacitance decay under different bending angles, and facile serial and parallel interconnection for creating high-voltage and high-capacitance integrated bipolar cells. Therefore, our proposed strategy will open many opportunites for patterning novel 2D mesoporous metal oxide nanosheets for high-performance microscale electrochemical energy storage devices.
✓ 已验证
DOI
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Dewei Wang,Lang Xu,Jiawang Nai,Xiaoxia Bai,Tao Sun
Controllable synthesis of nanocarbons with tunable morphologies from sustainable biomass through facile one-step route for ionic liquid based supercapacitors with high energy density and high power characteristics is of great interest but remains an ongoing challenge. Herein, we report the design and synthesis of nanocarbons with tunable morphologies (i.e., honeycomb-like porous carbons, carbon nanosheets and irregular-shaped carbon microparticles) from sustainable pine barks through reliable one-step chemical activation process. We found that the honeycomb-like porous carbons can be produced via direct KOH activation while the uniform carbon nanosheets were obtained by employing magnesium powder as the reactive self-sacrificing template. These three samples have different morphologies and textural properties render them totally disparate electrochemical performance in ionic liquid electrolyte. Benefiting from its distinct structural advantages, the as-obtained porous carbon nanosheets has excellent electrochemical capacitive performance including large specific capacitance (193 F/g at 1 A/g), superior rate capability (superior specific capacitance retention of 69.4% at 40 A/g), good cycling stability (good capacitance retention after 8000 cycles) and integrated high energy density& high power characteristics (excellent energy density of 55.8 Wh/kg even at an ultrahigh power density of 39.375 kW/kg). We believed that this work reported here not only provides a versatile strategy towards nanocarbons with tunable morphologies from sustainable biomass but also sheds some new light to efficiently regulate the morphologies of nanocarbons for electrochemical energy storage applications.
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2018
·Vol. 9
·期 1
·17
·
IF 3.504
Haiyang Shi,Weigen Chen,Fu Wan,Lingling Du,Shuhua Zhang,Weiran Zhou,Jiayi Zhang,Yingzhou Huang,Chengzhi Zhu
Accurate detection of dissolved aging features in transformer oil is the key to judging the aging degree of oil-paper insulation. In this work, in order to realize in situ detection of furfural dissolved in transformer oil, silver nanoparticles were self-assembled on the surface of gold film with P-aminophenylthiophenol (PATP) as a coupling agent. Rhodamine-6G (R6G) was used as the probe molecule to test the enhancement effect. By optimizing the molecular concentration, molecular deposition time, and silver sol deposition time of PATP, the nanoparticles were made more uniform and compact, and an enhanced substrate with rich hot spots was obtained. The optimum substrate was developed, and surface-enhanced Raman spectroscopy (SERS) detection of trace furfural dissolved in transformer oil was realized. The results showed that the substrate prepared under the conditions of 0.1 mol/L PATP, 5 h deposition in PATP and 12 h immersion in silver sol, had the best reinforcement effect (that is, uniform and compact particle arrangement and no particle clusters). By use of this substrate, the minimum detectable concentration of furfural in transformer oil was about 1.06 mg/L, which provides a new method for fast and nondestructive detection of transformer aging diagnosis.Keywords:surface-enhanced Raman spectroscopy;transformer aging;concentration detection
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）